Review



mouse ulbp  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    R&D Systems mouse ulbp
    Mouse Ulbp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mult-1/Recombinant+Mouse+ULBP-1%2FMULT-1+Protein/pm38342011-61-0-16
    Average 94 stars, based on 1 article reviews
    mouse ulbp - by Bioz Stars, 2026-11
    94/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: Group 1 innate lymphoid cell activation via recognition of NKG2D and liver resident macrophage MULT-1: Collaborated roles in triptolide induced hepatic immunotoxicity in mice.
    Article Snippet: 2-(4-Amidinophenyl)− 6-indolecarbamidine dihydrochloride (DAPI), Phorbol-12-myristate-13-acetate, Ionomycin and Brefeldin A were purchased from Beyotime Biotech (Shanghai, China). .. Mouse ULBP-1/MULT-1 and Rae-1 pan specific primary antibodies for flow cytometry or immunocytochemistry were purchased from R&D systems (Minneapolis, USA). .. Recombinant murine TNF-α was purchased from Peprotech, Thermo Fisher Scientific.

    Immunocytochemistry:

    Article Title: Group 1 innate lymphoid cell activation via recognition of NKG2D and liver resident macrophage MULT-1: Collaborated roles in triptolide induced hepatic immunotoxicity in mice.
    Article Snippet: 2-(4-Amidinophenyl)− 6-indolecarbamidine dihydrochloride (DAPI), Phorbol-12-myristate-13-acetate, Ionomycin and Brefeldin A were purchased from Beyotime Biotech (Shanghai, China). .. Mouse ULBP-1/MULT-1 and Rae-1 pan specific primary antibodies for flow cytometry or immunocytochemistry were purchased from R&D systems (Minneapolis, USA). .. Recombinant murine TNF-α was purchased from Peprotech, Thermo Fisher Scientific.

    In Vivo:

    Article Title: NK cells propagate T cell immunity following in situ tumor vaccination.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti CD45 PE-Cy7_Attune BioLegend RRID: AB_312978 Anti CD3 FITC_Attune BioLegend RRID: AB_312660 Anti CD4 BV510_Attune BioLegend RRID: AB_2561388 Anti CD8 PerCP-Cy5.5_Attune BioLegend RRID: AB_2075239 Anti NK1.1 BV605_Attune BioLegend RRID: AB_2562273 Anti CD25 BV711_Attune BioLegend RRID: AB_2564130 Anti FOXP3 BV421_Attune BioLegend RRID: AB_2565933 Anti CXCR3 APC_Attune BioLegend RRID: AB_2814077 Anti CD44 BV605_Attune BioLegend RRID: AB_2562451 Anti CD44 BV711_Attune BioLegend RRID: AB_2564214 Anti CD62L AF700_Attune BioLegend RRID: AB_493719 Anti H-2 FITC_Attune BioLegend RRID: AB_1236475 Anti TNFa BV711_Attune BioLegend RRID: AB_2629800 Anti TNFa PE_Attune BioLegend RRID: AB_315426 Anti IFNg APC_Attune BioLegend RRID: AB_315403 Anti PD-L1 PerCP-Cy5.5_Attune BioLegend RRID: AB_2629831 Anti PD-1 BV421_Attune BioLegend RRID: AB_10900085 Anti CD86 blocking Ab_Attune BioLegend RRID: AB_2888740 Anti CD86 PE_Attune BioLegend RRID: AB_2832567 Anti CTLA4 BV421_Attune BioLegend RRID: AB_2616790 Anti CTLA4 PE_Attune BioLegend RRID: AB_313254 Anti CD45 BUV395_Cytek BD RRID: AB_2651134 Anti CD4 BUV496_Cytek BD RRID: AB_2970666 Anti Ly6G BUV563_Cytek BD Cat# 612921 Anti CD11b BUV661_Cytek BD Cat# 612977 Anti CD8a_Cytek Invitrogen Cat# 368-0081-82 Anti B220_Cytek BioLegend RRID: AB_492877 Anti CD25_Cytek Invitrogen Cat# 69-0251-82; RRID: AB_2762802 Anti XCR1_Cytek BioLegend RRID: AB_566410 Anti NK1.1_Cytek BioLegend RRID: AB_2563286 Anti Siglec H_Cytek BD RRID: AB_2744231 Anti Siglec F_Cytek BD RRID: AB_2738833 Anti CD64 AF532_Cytek Novus Biologicals Cat# NB039151 Anti Ly6c PerCP_Cytek BioLegend RRID: AB_10900235 Anti MHC II BB700_Cytek BD RRID: AB_2743544 Anti CCR2 PE_Cytek BioLegend RRID: AB_2616981 Anti CD11c PE/Dazzle 594_Cytek BioLegend RRID: AB_2563654 Anti F4/80 PE/Cy5_Cytek BioLegend RRID: AB_893494 Anti CD172a PE/Cy7_Cytek BioLegend RRID: AB_2563545 Anti FOXP3 FITC_Cytek Invitrogen Cat# 11-5773-82; RRID: AB_465243 Anti CD3 APC_Cytek BioLegend RRID: AB_2561455 Anti CD90 AF700_Cytek R&D System Cat# FAB7335N-100UG Hu14.18K322A St. Jude Children’s Research Hospital N/A (Continued on next page) Cell Reports 42, 113556, December 26, 2023 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cetuximab (Erbitux, anti huEGFR antibody) Eli Lilly N/A Anti PD-L1 (in vivo) BioXcell RRID: AB10949073 Anti CTLA4 (in vivo) BioXcell RRID: AB10949609 CD8 depletion antibody (in vivo) BioXcell RRID: AB1125541 CD4 depletion antibody (in vivo) BioXcell RRID: AB1107636 NK depletion antibody (in vivo) BioXcell RRID: AB1107737 Biological samples Healthy donor PBMCs Stem Cell Cat# 200-0093 Patients PBMCs This paper UW16134 Chemicals, peptides, and recombinant proteins Recombinant mouse ULBP1 R&D System Cat# 2588-MU NF-kB transcription inhibitor (JSH 23) Abcam Cat# ab144824 IL2 National Cancer Institute Ro 23-6019 sNLS-spCas9-sNLS IDT Cat# 10017688 Critical commercial assays Multiplex immunoassay MilliporeSigma Cat# MCYTMAG-70K-PX32 IFNg ELISA assay BioLegend Cat# 430807 Single cell secretomes-human PhenomeX Cat# ISOCODE-1001-4 Single cell secretomes-mouse PhenomeX Cat# ISOCODE-1004-4 TruSeq RNA UD Indexes Illumina Cat# 20020591 TruSeq Stranded mRNA LP Illumina Cat# 20020595 CD4+ cell isolation Miltenyi Biotec Cat# 130-104-454 CD8+ cell isolation Miltenyi Biotec Cat# 130-104-075 NK cell isolation Miltenyi Biotec Cat# 130-115-818 Deposited data RNA seq This study GEO : GSE209945 Experimental models: Cell lines MOC2 (M2w) Brigham and Women’s Hospital and Dana-Farber Cancer Institute Dr. Ravindra Uppaluri MOC2-huEGFR (M2h) University of Wisconsin This study B16 Scripps Research Institute Dr. Ralph Reisfield B78 Scripps Research Institute Dr. Ralph Reisfield SCC7 La Jolla Institute Dr. Stephen Schoenberger Panc02 National Cancer institute This study Experimental models: Organisms/strains C57BL/6 Taconic B6-F IFNgtm1Ts Jackson Laboratory IMSR_JAX:002287 FOXP3tm3(DTR/GFP)Ayr Jackson Laboratory IMSR_JAX:016958 Klrk1tm1Dhr Jackson Laboratory IMSR_JAX:022733 Fcer1gtm1Rav Jackson Laboratory IMSR_JAX:002847 NOD-scid IL2Rgnull University of Wisconsin Dr. Paul Lambert Oligonucleotides Murine CD86 forward/reverse primers IDT N/A Murine HPRT forward/reverse primers IDT N/A Recombinant DNA CRISPR CD86 gRNA IDT Mm.Cas9.CD86.1.AA Software and algorithms FlowJo BD N/A (Continued on next page) 20 Cell Reports 42, 113556, December 26, 2023 ..

    Recombinant:

    Article Title: NK cells propagate T cell immunity following in situ tumor vaccination.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti CD45 PE-Cy7_Attune BioLegend RRID: AB_312978 Anti CD3 FITC_Attune BioLegend RRID: AB_312660 Anti CD4 BV510_Attune BioLegend RRID: AB_2561388 Anti CD8 PerCP-Cy5.5_Attune BioLegend RRID: AB_2075239 Anti NK1.1 BV605_Attune BioLegend RRID: AB_2562273 Anti CD25 BV711_Attune BioLegend RRID: AB_2564130 Anti FOXP3 BV421_Attune BioLegend RRID: AB_2565933 Anti CXCR3 APC_Attune BioLegend RRID: AB_2814077 Anti CD44 BV605_Attune BioLegend RRID: AB_2562451 Anti CD44 BV711_Attune BioLegend RRID: AB_2564214 Anti CD62L AF700_Attune BioLegend RRID: AB_493719 Anti H-2 FITC_Attune BioLegend RRID: AB_1236475 Anti TNFa BV711_Attune BioLegend RRID: AB_2629800 Anti TNFa PE_Attune BioLegend RRID: AB_315426 Anti IFNg APC_Attune BioLegend RRID: AB_315403 Anti PD-L1 PerCP-Cy5.5_Attune BioLegend RRID: AB_2629831 Anti PD-1 BV421_Attune BioLegend RRID: AB_10900085 Anti CD86 blocking Ab_Attune BioLegend RRID: AB_2888740 Anti CD86 PE_Attune BioLegend RRID: AB_2832567 Anti CTLA4 BV421_Attune BioLegend RRID: AB_2616790 Anti CTLA4 PE_Attune BioLegend RRID: AB_313254 Anti CD45 BUV395_Cytek BD RRID: AB_2651134 Anti CD4 BUV496_Cytek BD RRID: AB_2970666 Anti Ly6G BUV563_Cytek BD Cat# 612921 Anti CD11b BUV661_Cytek BD Cat# 612977 Anti CD8a_Cytek Invitrogen Cat# 368-0081-82 Anti B220_Cytek BioLegend RRID: AB_492877 Anti CD25_Cytek Invitrogen Cat# 69-0251-82; RRID: AB_2762802 Anti XCR1_Cytek BioLegend RRID: AB_566410 Anti NK1.1_Cytek BioLegend RRID: AB_2563286 Anti Siglec H_Cytek BD RRID: AB_2744231 Anti Siglec F_Cytek BD RRID: AB_2738833 Anti CD64 AF532_Cytek Novus Biologicals Cat# NB039151 Anti Ly6c PerCP_Cytek BioLegend RRID: AB_10900235 Anti MHC II BB700_Cytek BD RRID: AB_2743544 Anti CCR2 PE_Cytek BioLegend RRID: AB_2616981 Anti CD11c PE/Dazzle 594_Cytek BioLegend RRID: AB_2563654 Anti F4/80 PE/Cy5_Cytek BioLegend RRID: AB_893494 Anti CD172a PE/Cy7_Cytek BioLegend RRID: AB_2563545 Anti FOXP3 FITC_Cytek Invitrogen Cat# 11-5773-82; RRID: AB_465243 Anti CD3 APC_Cytek BioLegend RRID: AB_2561455 Anti CD90 AF700_Cytek R&D System Cat# FAB7335N-100UG Hu14.18K322A St. Jude Children’s Research Hospital N/A (Continued on next page) Cell Reports 42, 113556, December 26, 2023 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cetuximab (Erbitux, anti huEGFR antibody) Eli Lilly N/A Anti PD-L1 (in vivo) BioXcell RRID: AB10949073 Anti CTLA4 (in vivo) BioXcell RRID: AB10949609 CD8 depletion antibody (in vivo) BioXcell RRID: AB1125541 CD4 depletion antibody (in vivo) BioXcell RRID: AB1107636 NK depletion antibody (in vivo) BioXcell RRID: AB1107737 Biological samples Healthy donor PBMCs Stem Cell Cat# 200-0093 Patients PBMCs This paper UW16134 Chemicals, peptides, and recombinant proteins Recombinant mouse ULBP1 R&D System Cat# 2588-MU NF-kB transcription inhibitor (JSH 23) Abcam Cat# ab144824 IL2 National Cancer Institute Ro 23-6019 sNLS-spCas9-sNLS IDT Cat# 10017688 Critical commercial assays Multiplex immunoassay MilliporeSigma Cat# MCYTMAG-70K-PX32 IFNg ELISA assay BioLegend Cat# 430807 Single cell secretomes-human PhenomeX Cat# ISOCODE-1001-4 Single cell secretomes-mouse PhenomeX Cat# ISOCODE-1004-4 TruSeq RNA UD Indexes Illumina Cat# 20020591 TruSeq Stranded mRNA LP Illumina Cat# 20020595 CD4+ cell isolation Miltenyi Biotec Cat# 130-104-454 CD8+ cell isolation Miltenyi Biotec Cat# 130-104-075 NK cell isolation Miltenyi Biotec Cat# 130-115-818 Deposited data RNA seq This study GEO : GSE209945 Experimental models: Cell lines MOC2 (M2w) Brigham and Women’s Hospital and Dana-Farber Cancer Institute Dr. Ravindra Uppaluri MOC2-huEGFR (M2h) University of Wisconsin This study B16 Scripps Research Institute Dr. Ralph Reisfield B78 Scripps Research Institute Dr. Ralph Reisfield SCC7 La Jolla Institute Dr. Stephen Schoenberger Panc02 National Cancer institute This study Experimental models: Organisms/strains C57BL/6 Taconic B6-F IFNgtm1Ts Jackson Laboratory IMSR_JAX:002287 FOXP3tm3(DTR/GFP)Ayr Jackson Laboratory IMSR_JAX:016958 Klrk1tm1Dhr Jackson Laboratory IMSR_JAX:022733 Fcer1gtm1Rav Jackson Laboratory IMSR_JAX:002847 NOD-scid IL2Rgnull University of Wisconsin Dr. Paul Lambert Oligonucleotides Murine CD86 forward/reverse primers IDT N/A Murine HPRT forward/reverse primers IDT N/A Recombinant DNA CRISPR CD86 gRNA IDT Mm.Cas9.CD86.1.AA Software and algorithms FlowJo BD N/A (Continued on next page) 20 Cell Reports 42, 113556, December 26, 2023 ..

    Multiplex Assay:

    Article Title: NK cells propagate T cell immunity following in situ tumor vaccination.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti CD45 PE-Cy7_Attune BioLegend RRID: AB_312978 Anti CD3 FITC_Attune BioLegend RRID: AB_312660 Anti CD4 BV510_Attune BioLegend RRID: AB_2561388 Anti CD8 PerCP-Cy5.5_Attune BioLegend RRID: AB_2075239 Anti NK1.1 BV605_Attune BioLegend RRID: AB_2562273 Anti CD25 BV711_Attune BioLegend RRID: AB_2564130 Anti FOXP3 BV421_Attune BioLegend RRID: AB_2565933 Anti CXCR3 APC_Attune BioLegend RRID: AB_2814077 Anti CD44 BV605_Attune BioLegend RRID: AB_2562451 Anti CD44 BV711_Attune BioLegend RRID: AB_2564214 Anti CD62L AF700_Attune BioLegend RRID: AB_493719 Anti H-2 FITC_Attune BioLegend RRID: AB_1236475 Anti TNFa BV711_Attune BioLegend RRID: AB_2629800 Anti TNFa PE_Attune BioLegend RRID: AB_315426 Anti IFNg APC_Attune BioLegend RRID: AB_315403 Anti PD-L1 PerCP-Cy5.5_Attune BioLegend RRID: AB_2629831 Anti PD-1 BV421_Attune BioLegend RRID: AB_10900085 Anti CD86 blocking Ab_Attune BioLegend RRID: AB_2888740 Anti CD86 PE_Attune BioLegend RRID: AB_2832567 Anti CTLA4 BV421_Attune BioLegend RRID: AB_2616790 Anti CTLA4 PE_Attune BioLegend RRID: AB_313254 Anti CD45 BUV395_Cytek BD RRID: AB_2651134 Anti CD4 BUV496_Cytek BD RRID: AB_2970666 Anti Ly6G BUV563_Cytek BD Cat# 612921 Anti CD11b BUV661_Cytek BD Cat# 612977 Anti CD8a_Cytek Invitrogen Cat# 368-0081-82 Anti B220_Cytek BioLegend RRID: AB_492877 Anti CD25_Cytek Invitrogen Cat# 69-0251-82; RRID: AB_2762802 Anti XCR1_Cytek BioLegend RRID: AB_566410 Anti NK1.1_Cytek BioLegend RRID: AB_2563286 Anti Siglec H_Cytek BD RRID: AB_2744231 Anti Siglec F_Cytek BD RRID: AB_2738833 Anti CD64 AF532_Cytek Novus Biologicals Cat# NB039151 Anti Ly6c PerCP_Cytek BioLegend RRID: AB_10900235 Anti MHC II BB700_Cytek BD RRID: AB_2743544 Anti CCR2 PE_Cytek BioLegend RRID: AB_2616981 Anti CD11c PE/Dazzle 594_Cytek BioLegend RRID: AB_2563654 Anti F4/80 PE/Cy5_Cytek BioLegend RRID: AB_893494 Anti CD172a PE/Cy7_Cytek BioLegend RRID: AB_2563545 Anti FOXP3 FITC_Cytek Invitrogen Cat# 11-5773-82; RRID: AB_465243 Anti CD3 APC_Cytek BioLegend RRID: AB_2561455 Anti CD90 AF700_Cytek R&D System Cat# FAB7335N-100UG Hu14.18K322A St. Jude Children’s Research Hospital N/A (Continued on next page) Cell Reports 42, 113556, December 26, 2023 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cetuximab (Erbitux, anti huEGFR antibody) Eli Lilly N/A Anti PD-L1 (in vivo) BioXcell RRID: AB10949073 Anti CTLA4 (in vivo) BioXcell RRID: AB10949609 CD8 depletion antibody (in vivo) BioXcell RRID: AB1125541 CD4 depletion antibody (in vivo) BioXcell RRID: AB1107636 NK depletion antibody (in vivo) BioXcell RRID: AB1107737 Biological samples Healthy donor PBMCs Stem Cell Cat# 200-0093 Patients PBMCs This paper UW16134 Chemicals, peptides, and recombinant proteins Recombinant mouse ULBP1 R&D System Cat# 2588-MU NF-kB transcription inhibitor (JSH 23) Abcam Cat# ab144824 IL2 National Cancer Institute Ro 23-6019 sNLS-spCas9-sNLS IDT Cat# 10017688 Critical commercial assays Multiplex immunoassay MilliporeSigma Cat# MCYTMAG-70K-PX32 IFNg ELISA assay BioLegend Cat# 430807 Single cell secretomes-human PhenomeX Cat# ISOCODE-1001-4 Single cell secretomes-mouse PhenomeX Cat# ISOCODE-1004-4 TruSeq RNA UD Indexes Illumina Cat# 20020591 TruSeq Stranded mRNA LP Illumina Cat# 20020595 CD4+ cell isolation Miltenyi Biotec Cat# 130-104-454 CD8+ cell isolation Miltenyi Biotec Cat# 130-104-075 NK cell isolation Miltenyi Biotec Cat# 130-115-818 Deposited data RNA seq This study GEO : GSE209945 Experimental models: Cell lines MOC2 (M2w) Brigham and Women’s Hospital and Dana-Farber Cancer Institute Dr. Ravindra Uppaluri MOC2-huEGFR (M2h) University of Wisconsin This study B16 Scripps Research Institute Dr. Ralph Reisfield B78 Scripps Research Institute Dr. Ralph Reisfield SCC7 La Jolla Institute Dr. Stephen Schoenberger Panc02 National Cancer institute This study Experimental models: Organisms/strains C57BL/6 Taconic B6-F IFNgtm1Ts Jackson Laboratory IMSR_JAX:002287 FOXP3tm3(DTR/GFP)Ayr Jackson Laboratory IMSR_JAX:016958 Klrk1tm1Dhr Jackson Laboratory IMSR_JAX:022733 Fcer1gtm1Rav Jackson Laboratory IMSR_JAX:002847 NOD-scid IL2Rgnull University of Wisconsin Dr. Paul Lambert Oligonucleotides Murine CD86 forward/reverse primers IDT N/A Murine HPRT forward/reverse primers IDT N/A Recombinant DNA CRISPR CD86 gRNA IDT Mm.Cas9.CD86.1.AA Software and algorithms FlowJo BD N/A (Continued on next page) 20 Cell Reports 42, 113556, December 26, 2023 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: NK cells propagate T cell immunity following in situ tumor vaccination.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti CD45 PE-Cy7_Attune BioLegend RRID: AB_312978 Anti CD3 FITC_Attune BioLegend RRID: AB_312660 Anti CD4 BV510_Attune BioLegend RRID: AB_2561388 Anti CD8 PerCP-Cy5.5_Attune BioLegend RRID: AB_2075239 Anti NK1.1 BV605_Attune BioLegend RRID: AB_2562273 Anti CD25 BV711_Attune BioLegend RRID: AB_2564130 Anti FOXP3 BV421_Attune BioLegend RRID: AB_2565933 Anti CXCR3 APC_Attune BioLegend RRID: AB_2814077 Anti CD44 BV605_Attune BioLegend RRID: AB_2562451 Anti CD44 BV711_Attune BioLegend RRID: AB_2564214 Anti CD62L AF700_Attune BioLegend RRID: AB_493719 Anti H-2 FITC_Attune BioLegend RRID: AB_1236475 Anti TNFa BV711_Attune BioLegend RRID: AB_2629800 Anti TNFa PE_Attune BioLegend RRID: AB_315426 Anti IFNg APC_Attune BioLegend RRID: AB_315403 Anti PD-L1 PerCP-Cy5.5_Attune BioLegend RRID: AB_2629831 Anti PD-1 BV421_Attune BioLegend RRID: AB_10900085 Anti CD86 blocking Ab_Attune BioLegend RRID: AB_2888740 Anti CD86 PE_Attune BioLegend RRID: AB_2832567 Anti CTLA4 BV421_Attune BioLegend RRID: AB_2616790 Anti CTLA4 PE_Attune BioLegend RRID: AB_313254 Anti CD45 BUV395_Cytek BD RRID: AB_2651134 Anti CD4 BUV496_Cytek BD RRID: AB_2970666 Anti Ly6G BUV563_Cytek BD Cat# 612921 Anti CD11b BUV661_Cytek BD Cat# 612977 Anti CD8a_Cytek Invitrogen Cat# 368-0081-82 Anti B220_Cytek BioLegend RRID: AB_492877 Anti CD25_Cytek Invitrogen Cat# 69-0251-82; RRID: AB_2762802 Anti XCR1_Cytek BioLegend RRID: AB_566410 Anti NK1.1_Cytek BioLegend RRID: AB_2563286 Anti Siglec H_Cytek BD RRID: AB_2744231 Anti Siglec F_Cytek BD RRID: AB_2738833 Anti CD64 AF532_Cytek Novus Biologicals Cat# NB039151 Anti Ly6c PerCP_Cytek BioLegend RRID: AB_10900235 Anti MHC II BB700_Cytek BD RRID: AB_2743544 Anti CCR2 PE_Cytek BioLegend RRID: AB_2616981 Anti CD11c PE/Dazzle 594_Cytek BioLegend RRID: AB_2563654 Anti F4/80 PE/Cy5_Cytek BioLegend RRID: AB_893494 Anti CD172a PE/Cy7_Cytek BioLegend RRID: AB_2563545 Anti FOXP3 FITC_Cytek Invitrogen Cat# 11-5773-82; RRID: AB_465243 Anti CD3 APC_Cytek BioLegend RRID: AB_2561455 Anti CD90 AF700_Cytek R&D System Cat# FAB7335N-100UG Hu14.18K322A St. Jude Children’s Research Hospital N/A (Continued on next page) Cell Reports 42, 113556, December 26, 2023 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cetuximab (Erbitux, anti huEGFR antibody) Eli Lilly N/A Anti PD-L1 (in vivo) BioXcell RRID: AB10949073 Anti CTLA4 (in vivo) BioXcell RRID: AB10949609 CD8 depletion antibody (in vivo) BioXcell RRID: AB1125541 CD4 depletion antibody (in vivo) BioXcell RRID: AB1107636 NK depletion antibody (in vivo) BioXcell RRID: AB1107737 Biological samples Healthy donor PBMCs Stem Cell Cat# 200-0093 Patients PBMCs This paper UW16134 Chemicals, peptides, and recombinant proteins Recombinant mouse ULBP1 R&D System Cat# 2588-MU NF-kB transcription inhibitor (JSH 23) Abcam Cat# ab144824 IL2 National Cancer Institute Ro 23-6019 sNLS-spCas9-sNLS IDT Cat# 10017688 Critical commercial assays Multiplex immunoassay MilliporeSigma Cat# MCYTMAG-70K-PX32 IFNg ELISA assay BioLegend Cat# 430807 Single cell secretomes-human PhenomeX Cat# ISOCODE-1001-4 Single cell secretomes-mouse PhenomeX Cat# ISOCODE-1004-4 TruSeq RNA UD Indexes Illumina Cat# 20020591 TruSeq Stranded mRNA LP Illumina Cat# 20020595 CD4+ cell isolation Miltenyi Biotec Cat# 130-104-454 CD8+ cell isolation Miltenyi Biotec Cat# 130-104-075 NK cell isolation Miltenyi Biotec Cat# 130-115-818 Deposited data RNA seq This study GEO : GSE209945 Experimental models: Cell lines MOC2 (M2w) Brigham and Women’s Hospital and Dana-Farber Cancer Institute Dr. Ravindra Uppaluri MOC2-huEGFR (M2h) University of Wisconsin This study B16 Scripps Research Institute Dr. Ralph Reisfield B78 Scripps Research Institute Dr. Ralph Reisfield SCC7 La Jolla Institute Dr. Stephen Schoenberger Panc02 National Cancer institute This study Experimental models: Organisms/strains C57BL/6 Taconic B6-F IFNgtm1Ts Jackson Laboratory IMSR_JAX:002287 FOXP3tm3(DTR/GFP)Ayr Jackson Laboratory IMSR_JAX:016958 Klrk1tm1Dhr Jackson Laboratory IMSR_JAX:022733 Fcer1gtm1Rav Jackson Laboratory IMSR_JAX:002847 NOD-scid IL2Rgnull University of Wisconsin Dr. Paul Lambert Oligonucleotides Murine CD86 forward/reverse primers IDT N/A Murine HPRT forward/reverse primers IDT N/A Recombinant DNA CRISPR CD86 gRNA IDT Mm.Cas9.CD86.1.AA Software and algorithms FlowJo BD N/A (Continued on next page) 20 Cell Reports 42, 113556, December 26, 2023 ..

    Single Cell:

    Article Title: NK cells propagate T cell immunity following in situ tumor vaccination.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti CD45 PE-Cy7_Attune BioLegend RRID: AB_312978 Anti CD3 FITC_Attune BioLegend RRID: AB_312660 Anti CD4 BV510_Attune BioLegend RRID: AB_2561388 Anti CD8 PerCP-Cy5.5_Attune BioLegend RRID: AB_2075239 Anti NK1.1 BV605_Attune BioLegend RRID: AB_2562273 Anti CD25 BV711_Attune BioLegend RRID: AB_2564130 Anti FOXP3 BV421_Attune BioLegend RRID: AB_2565933 Anti CXCR3 APC_Attune BioLegend RRID: AB_2814077 Anti CD44 BV605_Attune BioLegend RRID: AB_2562451 Anti CD44 BV711_Attune BioLegend RRID: AB_2564214 Anti CD62L AF700_Attune BioLegend RRID: AB_493719 Anti H-2 FITC_Attune BioLegend RRID: AB_1236475 Anti TNFa BV711_Attune BioLegend RRID: AB_2629800 Anti TNFa PE_Attune BioLegend RRID: AB_315426 Anti IFNg APC_Attune BioLegend RRID: AB_315403 Anti PD-L1 PerCP-Cy5.5_Attune BioLegend RRID: AB_2629831 Anti PD-1 BV421_Attune BioLegend RRID: AB_10900085 Anti CD86 blocking Ab_Attune BioLegend RRID: AB_2888740 Anti CD86 PE_Attune BioLegend RRID: AB_2832567 Anti CTLA4 BV421_Attune BioLegend RRID: AB_2616790 Anti CTLA4 PE_Attune BioLegend RRID: AB_313254 Anti CD45 BUV395_Cytek BD RRID: AB_2651134 Anti CD4 BUV496_Cytek BD RRID: AB_2970666 Anti Ly6G BUV563_Cytek BD Cat# 612921 Anti CD11b BUV661_Cytek BD Cat# 612977 Anti CD8a_Cytek Invitrogen Cat# 368-0081-82 Anti B220_Cytek BioLegend RRID: AB_492877 Anti CD25_Cytek Invitrogen Cat# 69-0251-82; RRID: AB_2762802 Anti XCR1_Cytek BioLegend RRID: AB_566410 Anti NK1.1_Cytek BioLegend RRID: AB_2563286 Anti Siglec H_Cytek BD RRID: AB_2744231 Anti Siglec F_Cytek BD RRID: AB_2738833 Anti CD64 AF532_Cytek Novus Biologicals Cat# NB039151 Anti Ly6c PerCP_Cytek BioLegend RRID: AB_10900235 Anti MHC II BB700_Cytek BD RRID: AB_2743544 Anti CCR2 PE_Cytek BioLegend RRID: AB_2616981 Anti CD11c PE/Dazzle 594_Cytek BioLegend RRID: AB_2563654 Anti F4/80 PE/Cy5_Cytek BioLegend RRID: AB_893494 Anti CD172a PE/Cy7_Cytek BioLegend RRID: AB_2563545 Anti FOXP3 FITC_Cytek Invitrogen Cat# 11-5773-82; RRID: AB_465243 Anti CD3 APC_Cytek BioLegend RRID: AB_2561455 Anti CD90 AF700_Cytek R&D System Cat# FAB7335N-100UG Hu14.18K322A St. Jude Children’s Research Hospital N/A (Continued on next page) Cell Reports 42, 113556, December 26, 2023 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cetuximab (Erbitux, anti huEGFR antibody) Eli Lilly N/A Anti PD-L1 (in vivo) BioXcell RRID: AB10949073 Anti CTLA4 (in vivo) BioXcell RRID: AB10949609 CD8 depletion antibody (in vivo) BioXcell RRID: AB1125541 CD4 depletion antibody (in vivo) BioXcell RRID: AB1107636 NK depletion antibody (in vivo) BioXcell RRID: AB1107737 Biological samples Healthy donor PBMCs Stem Cell Cat# 200-0093 Patients PBMCs This paper UW16134 Chemicals, peptides, and recombinant proteins Recombinant mouse ULBP1 R&D System Cat# 2588-MU NF-kB transcription inhibitor (JSH 23) Abcam Cat# ab144824 IL2 National Cancer Institute Ro 23-6019 sNLS-spCas9-sNLS IDT Cat# 10017688 Critical commercial assays Multiplex immunoassay MilliporeSigma Cat# MCYTMAG-70K-PX32 IFNg ELISA assay BioLegend Cat# 430807 Single cell secretomes-human PhenomeX Cat# ISOCODE-1001-4 Single cell secretomes-mouse PhenomeX Cat# ISOCODE-1004-4 TruSeq RNA UD Indexes Illumina Cat# 20020591 TruSeq Stranded mRNA LP Illumina Cat# 20020595 CD4+ cell isolation Miltenyi Biotec Cat# 130-104-454 CD8+ cell isolation Miltenyi Biotec Cat# 130-104-075 NK cell isolation Miltenyi Biotec Cat# 130-115-818 Deposited data RNA seq This study GEO : GSE209945 Experimental models: Cell lines MOC2 (M2w) Brigham and Women’s Hospital and Dana-Farber Cancer Institute Dr. Ravindra Uppaluri MOC2-huEGFR (M2h) University of Wisconsin This study B16 Scripps Research Institute Dr. Ralph Reisfield B78 Scripps Research Institute Dr. Ralph Reisfield SCC7 La Jolla Institute Dr. Stephen Schoenberger Panc02 National Cancer institute This study Experimental models: Organisms/strains C57BL/6 Taconic B6-F IFNgtm1Ts Jackson Laboratory IMSR_JAX:002287 FOXP3tm3(DTR/GFP)Ayr Jackson Laboratory IMSR_JAX:016958 Klrk1tm1Dhr Jackson Laboratory IMSR_JAX:022733 Fcer1gtm1Rav Jackson Laboratory IMSR_JAX:002847 NOD-scid IL2Rgnull University of Wisconsin Dr. Paul Lambert Oligonucleotides Murine CD86 forward/reverse primers IDT N/A Murine HPRT forward/reverse primers IDT N/A Recombinant DNA CRISPR CD86 gRNA IDT Mm.Cas9.CD86.1.AA Software and algorithms FlowJo BD N/A (Continued on next page) 20 Cell Reports 42, 113556, December 26, 2023 ..

    Cell Isolation:

    Article Title: NK cells propagate T cell immunity following in situ tumor vaccination.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti CD45 PE-Cy7_Attune BioLegend RRID: AB_312978 Anti CD3 FITC_Attune BioLegend RRID: AB_312660 Anti CD4 BV510_Attune BioLegend RRID: AB_2561388 Anti CD8 PerCP-Cy5.5_Attune BioLegend RRID: AB_2075239 Anti NK1.1 BV605_Attune BioLegend RRID: AB_2562273 Anti CD25 BV711_Attune BioLegend RRID: AB_2564130 Anti FOXP3 BV421_Attune BioLegend RRID: AB_2565933 Anti CXCR3 APC_Attune BioLegend RRID: AB_2814077 Anti CD44 BV605_Attune BioLegend RRID: AB_2562451 Anti CD44 BV711_Attune BioLegend RRID: AB_2564214 Anti CD62L AF700_Attune BioLegend RRID: AB_493719 Anti H-2 FITC_Attune BioLegend RRID: AB_1236475 Anti TNFa BV711_Attune BioLegend RRID: AB_2629800 Anti TNFa PE_Attune BioLegend RRID: AB_315426 Anti IFNg APC_Attune BioLegend RRID: AB_315403 Anti PD-L1 PerCP-Cy5.5_Attune BioLegend RRID: AB_2629831 Anti PD-1 BV421_Attune BioLegend RRID: AB_10900085 Anti CD86 blocking Ab_Attune BioLegend RRID: AB_2888740 Anti CD86 PE_Attune BioLegend RRID: AB_2832567 Anti CTLA4 BV421_Attune BioLegend RRID: AB_2616790 Anti CTLA4 PE_Attune BioLegend RRID: AB_313254 Anti CD45 BUV395_Cytek BD RRID: AB_2651134 Anti CD4 BUV496_Cytek BD RRID: AB_2970666 Anti Ly6G BUV563_Cytek BD Cat# 612921 Anti CD11b BUV661_Cytek BD Cat# 612977 Anti CD8a_Cytek Invitrogen Cat# 368-0081-82 Anti B220_Cytek BioLegend RRID: AB_492877 Anti CD25_Cytek Invitrogen Cat# 69-0251-82; RRID: AB_2762802 Anti XCR1_Cytek BioLegend RRID: AB_566410 Anti NK1.1_Cytek BioLegend RRID: AB_2563286 Anti Siglec H_Cytek BD RRID: AB_2744231 Anti Siglec F_Cytek BD RRID: AB_2738833 Anti CD64 AF532_Cytek Novus Biologicals Cat# NB039151 Anti Ly6c PerCP_Cytek BioLegend RRID: AB_10900235 Anti MHC II BB700_Cytek BD RRID: AB_2743544 Anti CCR2 PE_Cytek BioLegend RRID: AB_2616981 Anti CD11c PE/Dazzle 594_Cytek BioLegend RRID: AB_2563654 Anti F4/80 PE/Cy5_Cytek BioLegend RRID: AB_893494 Anti CD172a PE/Cy7_Cytek BioLegend RRID: AB_2563545 Anti FOXP3 FITC_Cytek Invitrogen Cat# 11-5773-82; RRID: AB_465243 Anti CD3 APC_Cytek BioLegend RRID: AB_2561455 Anti CD90 AF700_Cytek R&D System Cat# FAB7335N-100UG Hu14.18K322A St. Jude Children’s Research Hospital N/A (Continued on next page) Cell Reports 42, 113556, December 26, 2023 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cetuximab (Erbitux, anti huEGFR antibody) Eli Lilly N/A Anti PD-L1 (in vivo) BioXcell RRID: AB10949073 Anti CTLA4 (in vivo) BioXcell RRID: AB10949609 CD8 depletion antibody (in vivo) BioXcell RRID: AB1125541 CD4 depletion antibody (in vivo) BioXcell RRID: AB1107636 NK depletion antibody (in vivo) BioXcell RRID: AB1107737 Biological samples Healthy donor PBMCs Stem Cell Cat# 200-0093 Patients PBMCs This paper UW16134 Chemicals, peptides, and recombinant proteins Recombinant mouse ULBP1 R&D System Cat# 2588-MU NF-kB transcription inhibitor (JSH 23) Abcam Cat# ab144824 IL2 National Cancer Institute Ro 23-6019 sNLS-spCas9-sNLS IDT Cat# 10017688 Critical commercial assays Multiplex immunoassay MilliporeSigma Cat# MCYTMAG-70K-PX32 IFNg ELISA assay BioLegend Cat# 430807 Single cell secretomes-human PhenomeX Cat# ISOCODE-1001-4 Single cell secretomes-mouse PhenomeX Cat# ISOCODE-1004-4 TruSeq RNA UD Indexes Illumina Cat# 20020591 TruSeq Stranded mRNA LP Illumina Cat# 20020595 CD4+ cell isolation Miltenyi Biotec Cat# 130-104-454 CD8+ cell isolation Miltenyi Biotec Cat# 130-104-075 NK cell isolation Miltenyi Biotec Cat# 130-115-818 Deposited data RNA seq This study GEO : GSE209945 Experimental models: Cell lines MOC2 (M2w) Brigham and Women’s Hospital and Dana-Farber Cancer Institute Dr. Ravindra Uppaluri MOC2-huEGFR (M2h) University of Wisconsin This study B16 Scripps Research Institute Dr. Ralph Reisfield B78 Scripps Research Institute Dr. Ralph Reisfield SCC7 La Jolla Institute Dr. Stephen Schoenberger Panc02 National Cancer institute This study Experimental models: Organisms/strains C57BL/6 Taconic B6-F IFNgtm1Ts Jackson Laboratory IMSR_JAX:002287 FOXP3tm3(DTR/GFP)Ayr Jackson Laboratory IMSR_JAX:016958 Klrk1tm1Dhr Jackson Laboratory IMSR_JAX:022733 Fcer1gtm1Rav Jackson Laboratory IMSR_JAX:002847 NOD-scid IL2Rgnull University of Wisconsin Dr. Paul Lambert Oligonucleotides Murine CD86 forward/reverse primers IDT N/A Murine HPRT forward/reverse primers IDT N/A Recombinant DNA CRISPR CD86 gRNA IDT Mm.Cas9.CD86.1.AA Software and algorithms FlowJo BD N/A (Continued on next page) 20 Cell Reports 42, 113556, December 26, 2023 ..

    RNA Sequencing:

    Article Title: NK cells propagate T cell immunity following in situ tumor vaccination.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti CD45 PE-Cy7_Attune BioLegend RRID: AB_312978 Anti CD3 FITC_Attune BioLegend RRID: AB_312660 Anti CD4 BV510_Attune BioLegend RRID: AB_2561388 Anti CD8 PerCP-Cy5.5_Attune BioLegend RRID: AB_2075239 Anti NK1.1 BV605_Attune BioLegend RRID: AB_2562273 Anti CD25 BV711_Attune BioLegend RRID: AB_2564130 Anti FOXP3 BV421_Attune BioLegend RRID: AB_2565933 Anti CXCR3 APC_Attune BioLegend RRID: AB_2814077 Anti CD44 BV605_Attune BioLegend RRID: AB_2562451 Anti CD44 BV711_Attune BioLegend RRID: AB_2564214 Anti CD62L AF700_Attune BioLegend RRID: AB_493719 Anti H-2 FITC_Attune BioLegend RRID: AB_1236475 Anti TNFa BV711_Attune BioLegend RRID: AB_2629800 Anti TNFa PE_Attune BioLegend RRID: AB_315426 Anti IFNg APC_Attune BioLegend RRID: AB_315403 Anti PD-L1 PerCP-Cy5.5_Attune BioLegend RRID: AB_2629831 Anti PD-1 BV421_Attune BioLegend RRID: AB_10900085 Anti CD86 blocking Ab_Attune BioLegend RRID: AB_2888740 Anti CD86 PE_Attune BioLegend RRID: AB_2832567 Anti CTLA4 BV421_Attune BioLegend RRID: AB_2616790 Anti CTLA4 PE_Attune BioLegend RRID: AB_313254 Anti CD45 BUV395_Cytek BD RRID: AB_2651134 Anti CD4 BUV496_Cytek BD RRID: AB_2970666 Anti Ly6G BUV563_Cytek BD Cat# 612921 Anti CD11b BUV661_Cytek BD Cat# 612977 Anti CD8a_Cytek Invitrogen Cat# 368-0081-82 Anti B220_Cytek BioLegend RRID: AB_492877 Anti CD25_Cytek Invitrogen Cat# 69-0251-82; RRID: AB_2762802 Anti XCR1_Cytek BioLegend RRID: AB_566410 Anti NK1.1_Cytek BioLegend RRID: AB_2563286 Anti Siglec H_Cytek BD RRID: AB_2744231 Anti Siglec F_Cytek BD RRID: AB_2738833 Anti CD64 AF532_Cytek Novus Biologicals Cat# NB039151 Anti Ly6c PerCP_Cytek BioLegend RRID: AB_10900235 Anti MHC II BB700_Cytek BD RRID: AB_2743544 Anti CCR2 PE_Cytek BioLegend RRID: AB_2616981 Anti CD11c PE/Dazzle 594_Cytek BioLegend RRID: AB_2563654 Anti F4/80 PE/Cy5_Cytek BioLegend RRID: AB_893494 Anti CD172a PE/Cy7_Cytek BioLegend RRID: AB_2563545 Anti FOXP3 FITC_Cytek Invitrogen Cat# 11-5773-82; RRID: AB_465243 Anti CD3 APC_Cytek BioLegend RRID: AB_2561455 Anti CD90 AF700_Cytek R&D System Cat# FAB7335N-100UG Hu14.18K322A St. Jude Children’s Research Hospital N/A (Continued on next page) Cell Reports 42, 113556, December 26, 2023 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cetuximab (Erbitux, anti huEGFR antibody) Eli Lilly N/A Anti PD-L1 (in vivo) BioXcell RRID: AB10949073 Anti CTLA4 (in vivo) BioXcell RRID: AB10949609 CD8 depletion antibody (in vivo) BioXcell RRID: AB1125541 CD4 depletion antibody (in vivo) BioXcell RRID: AB1107636 NK depletion antibody (in vivo) BioXcell RRID: AB1107737 Biological samples Healthy donor PBMCs Stem Cell Cat# 200-0093 Patients PBMCs This paper UW16134 Chemicals, peptides, and recombinant proteins Recombinant mouse ULBP1 R&D System Cat# 2588-MU NF-kB transcription inhibitor (JSH 23) Abcam Cat# ab144824 IL2 National Cancer Institute Ro 23-6019 sNLS-spCas9-sNLS IDT Cat# 10017688 Critical commercial assays Multiplex immunoassay MilliporeSigma Cat# MCYTMAG-70K-PX32 IFNg ELISA assay BioLegend Cat# 430807 Single cell secretomes-human PhenomeX Cat# ISOCODE-1001-4 Single cell secretomes-mouse PhenomeX Cat# ISOCODE-1004-4 TruSeq RNA UD Indexes Illumina Cat# 20020591 TruSeq Stranded mRNA LP Illumina Cat# 20020595 CD4+ cell isolation Miltenyi Biotec Cat# 130-104-454 CD8+ cell isolation Miltenyi Biotec Cat# 130-104-075 NK cell isolation Miltenyi Biotec Cat# 130-115-818 Deposited data RNA seq This study GEO : GSE209945 Experimental models: Cell lines MOC2 (M2w) Brigham and Women’s Hospital and Dana-Farber Cancer Institute Dr. Ravindra Uppaluri MOC2-huEGFR (M2h) University of Wisconsin This study B16 Scripps Research Institute Dr. Ralph Reisfield B78 Scripps Research Institute Dr. Ralph Reisfield SCC7 La Jolla Institute Dr. Stephen Schoenberger Panc02 National Cancer institute This study Experimental models: Organisms/strains C57BL/6 Taconic B6-F IFNgtm1Ts Jackson Laboratory IMSR_JAX:002287 FOXP3tm3(DTR/GFP)Ayr Jackson Laboratory IMSR_JAX:016958 Klrk1tm1Dhr Jackson Laboratory IMSR_JAX:022733 Fcer1gtm1Rav Jackson Laboratory IMSR_JAX:002847 NOD-scid IL2Rgnull University of Wisconsin Dr. Paul Lambert Oligonucleotides Murine CD86 forward/reverse primers IDT N/A Murine HPRT forward/reverse primers IDT N/A Recombinant DNA CRISPR CD86 gRNA IDT Mm.Cas9.CD86.1.AA Software and algorithms FlowJo BD N/A (Continued on next page) 20 Cell Reports 42, 113556, December 26, 2023 ..

    CRISPR:

    Article Title: NK cells propagate T cell immunity following in situ tumor vaccination.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti CD45 PE-Cy7_Attune BioLegend RRID: AB_312978 Anti CD3 FITC_Attune BioLegend RRID: AB_312660 Anti CD4 BV510_Attune BioLegend RRID: AB_2561388 Anti CD8 PerCP-Cy5.5_Attune BioLegend RRID: AB_2075239 Anti NK1.1 BV605_Attune BioLegend RRID: AB_2562273 Anti CD25 BV711_Attune BioLegend RRID: AB_2564130 Anti FOXP3 BV421_Attune BioLegend RRID: AB_2565933 Anti CXCR3 APC_Attune BioLegend RRID: AB_2814077 Anti CD44 BV605_Attune BioLegend RRID: AB_2562451 Anti CD44 BV711_Attune BioLegend RRID: AB_2564214 Anti CD62L AF700_Attune BioLegend RRID: AB_493719 Anti H-2 FITC_Attune BioLegend RRID: AB_1236475 Anti TNFa BV711_Attune BioLegend RRID: AB_2629800 Anti TNFa PE_Attune BioLegend RRID: AB_315426 Anti IFNg APC_Attune BioLegend RRID: AB_315403 Anti PD-L1 PerCP-Cy5.5_Attune BioLegend RRID: AB_2629831 Anti PD-1 BV421_Attune BioLegend RRID: AB_10900085 Anti CD86 blocking Ab_Attune BioLegend RRID: AB_2888740 Anti CD86 PE_Attune BioLegend RRID: AB_2832567 Anti CTLA4 BV421_Attune BioLegend RRID: AB_2616790 Anti CTLA4 PE_Attune BioLegend RRID: AB_313254 Anti CD45 BUV395_Cytek BD RRID: AB_2651134 Anti CD4 BUV496_Cytek BD RRID: AB_2970666 Anti Ly6G BUV563_Cytek BD Cat# 612921 Anti CD11b BUV661_Cytek BD Cat# 612977 Anti CD8a_Cytek Invitrogen Cat# 368-0081-82 Anti B220_Cytek BioLegend RRID: AB_492877 Anti CD25_Cytek Invitrogen Cat# 69-0251-82; RRID: AB_2762802 Anti XCR1_Cytek BioLegend RRID: AB_566410 Anti NK1.1_Cytek BioLegend RRID: AB_2563286 Anti Siglec H_Cytek BD RRID: AB_2744231 Anti Siglec F_Cytek BD RRID: AB_2738833 Anti CD64 AF532_Cytek Novus Biologicals Cat# NB039151 Anti Ly6c PerCP_Cytek BioLegend RRID: AB_10900235 Anti MHC II BB700_Cytek BD RRID: AB_2743544 Anti CCR2 PE_Cytek BioLegend RRID: AB_2616981 Anti CD11c PE/Dazzle 594_Cytek BioLegend RRID: AB_2563654 Anti F4/80 PE/Cy5_Cytek BioLegend RRID: AB_893494 Anti CD172a PE/Cy7_Cytek BioLegend RRID: AB_2563545 Anti FOXP3 FITC_Cytek Invitrogen Cat# 11-5773-82; RRID: AB_465243 Anti CD3 APC_Cytek BioLegend RRID: AB_2561455 Anti CD90 AF700_Cytek R&D System Cat# FAB7335N-100UG Hu14.18K322A St. Jude Children’s Research Hospital N/A (Continued on next page) Cell Reports 42, 113556, December 26, 2023 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cetuximab (Erbitux, anti huEGFR antibody) Eli Lilly N/A Anti PD-L1 (in vivo) BioXcell RRID: AB10949073 Anti CTLA4 (in vivo) BioXcell RRID: AB10949609 CD8 depletion antibody (in vivo) BioXcell RRID: AB1125541 CD4 depletion antibody (in vivo) BioXcell RRID: AB1107636 NK depletion antibody (in vivo) BioXcell RRID: AB1107737 Biological samples Healthy donor PBMCs Stem Cell Cat# 200-0093 Patients PBMCs This paper UW16134 Chemicals, peptides, and recombinant proteins Recombinant mouse ULBP1 R&D System Cat# 2588-MU NF-kB transcription inhibitor (JSH 23) Abcam Cat# ab144824 IL2 National Cancer Institute Ro 23-6019 sNLS-spCas9-sNLS IDT Cat# 10017688 Critical commercial assays Multiplex immunoassay MilliporeSigma Cat# MCYTMAG-70K-PX32 IFNg ELISA assay BioLegend Cat# 430807 Single cell secretomes-human PhenomeX Cat# ISOCODE-1001-4 Single cell secretomes-mouse PhenomeX Cat# ISOCODE-1004-4 TruSeq RNA UD Indexes Illumina Cat# 20020591 TruSeq Stranded mRNA LP Illumina Cat# 20020595 CD4+ cell isolation Miltenyi Biotec Cat# 130-104-454 CD8+ cell isolation Miltenyi Biotec Cat# 130-104-075 NK cell isolation Miltenyi Biotec Cat# 130-115-818 Deposited data RNA seq This study GEO : GSE209945 Experimental models: Cell lines MOC2 (M2w) Brigham and Women’s Hospital and Dana-Farber Cancer Institute Dr. Ravindra Uppaluri MOC2-huEGFR (M2h) University of Wisconsin This study B16 Scripps Research Institute Dr. Ralph Reisfield B78 Scripps Research Institute Dr. Ralph Reisfield SCC7 La Jolla Institute Dr. Stephen Schoenberger Panc02 National Cancer institute This study Experimental models: Organisms/strains C57BL/6 Taconic B6-F IFNgtm1Ts Jackson Laboratory IMSR_JAX:002287 FOXP3tm3(DTR/GFP)Ayr Jackson Laboratory IMSR_JAX:016958 Klrk1tm1Dhr Jackson Laboratory IMSR_JAX:022733 Fcer1gtm1Rav Jackson Laboratory IMSR_JAX:002847 NOD-scid IL2Rgnull University of Wisconsin Dr. Paul Lambert Oligonucleotides Murine CD86 forward/reverse primers IDT N/A Murine HPRT forward/reverse primers IDT N/A Recombinant DNA CRISPR CD86 gRNA IDT Mm.Cas9.CD86.1.AA Software and algorithms FlowJo BD N/A (Continued on next page) 20 Cell Reports 42, 113556, December 26, 2023 ..

    Software:

    Article Title: NK cells propagate T cell immunity following in situ tumor vaccination.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti CD45 PE-Cy7_Attune BioLegend RRID: AB_312978 Anti CD3 FITC_Attune BioLegend RRID: AB_312660 Anti CD4 BV510_Attune BioLegend RRID: AB_2561388 Anti CD8 PerCP-Cy5.5_Attune BioLegend RRID: AB_2075239 Anti NK1.1 BV605_Attune BioLegend RRID: AB_2562273 Anti CD25 BV711_Attune BioLegend RRID: AB_2564130 Anti FOXP3 BV421_Attune BioLegend RRID: AB_2565933 Anti CXCR3 APC_Attune BioLegend RRID: AB_2814077 Anti CD44 BV605_Attune BioLegend RRID: AB_2562451 Anti CD44 BV711_Attune BioLegend RRID: AB_2564214 Anti CD62L AF700_Attune BioLegend RRID: AB_493719 Anti H-2 FITC_Attune BioLegend RRID: AB_1236475 Anti TNFa BV711_Attune BioLegend RRID: AB_2629800 Anti TNFa PE_Attune BioLegend RRID: AB_315426 Anti IFNg APC_Attune BioLegend RRID: AB_315403 Anti PD-L1 PerCP-Cy5.5_Attune BioLegend RRID: AB_2629831 Anti PD-1 BV421_Attune BioLegend RRID: AB_10900085 Anti CD86 blocking Ab_Attune BioLegend RRID: AB_2888740 Anti CD86 PE_Attune BioLegend RRID: AB_2832567 Anti CTLA4 BV421_Attune BioLegend RRID: AB_2616790 Anti CTLA4 PE_Attune BioLegend RRID: AB_313254 Anti CD45 BUV395_Cytek BD RRID: AB_2651134 Anti CD4 BUV496_Cytek BD RRID: AB_2970666 Anti Ly6G BUV563_Cytek BD Cat# 612921 Anti CD11b BUV661_Cytek BD Cat# 612977 Anti CD8a_Cytek Invitrogen Cat# 368-0081-82 Anti B220_Cytek BioLegend RRID: AB_492877 Anti CD25_Cytek Invitrogen Cat# 69-0251-82; RRID: AB_2762802 Anti XCR1_Cytek BioLegend RRID: AB_566410 Anti NK1.1_Cytek BioLegend RRID: AB_2563286 Anti Siglec H_Cytek BD RRID: AB_2744231 Anti Siglec F_Cytek BD RRID: AB_2738833 Anti CD64 AF532_Cytek Novus Biologicals Cat# NB039151 Anti Ly6c PerCP_Cytek BioLegend RRID: AB_10900235 Anti MHC II BB700_Cytek BD RRID: AB_2743544 Anti CCR2 PE_Cytek BioLegend RRID: AB_2616981 Anti CD11c PE/Dazzle 594_Cytek BioLegend RRID: AB_2563654 Anti F4/80 PE/Cy5_Cytek BioLegend RRID: AB_893494 Anti CD172a PE/Cy7_Cytek BioLegend RRID: AB_2563545 Anti FOXP3 FITC_Cytek Invitrogen Cat# 11-5773-82; RRID: AB_465243 Anti CD3 APC_Cytek BioLegend RRID: AB_2561455 Anti CD90 AF700_Cytek R&D System Cat# FAB7335N-100UG Hu14.18K322A St. Jude Children’s Research Hospital N/A (Continued on next page) Cell Reports 42, 113556, December 26, 2023 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cetuximab (Erbitux, anti huEGFR antibody) Eli Lilly N/A Anti PD-L1 (in vivo) BioXcell RRID: AB10949073 Anti CTLA4 (in vivo) BioXcell RRID: AB10949609 CD8 depletion antibody (in vivo) BioXcell RRID: AB1125541 CD4 depletion antibody (in vivo) BioXcell RRID: AB1107636 NK depletion antibody (in vivo) BioXcell RRID: AB1107737 Biological samples Healthy donor PBMCs Stem Cell Cat# 200-0093 Patients PBMCs This paper UW16134 Chemicals, peptides, and recombinant proteins Recombinant mouse ULBP1 R&D System Cat# 2588-MU NF-kB transcription inhibitor (JSH 23) Abcam Cat# ab144824 IL2 National Cancer Institute Ro 23-6019 sNLS-spCas9-sNLS IDT Cat# 10017688 Critical commercial assays Multiplex immunoassay MilliporeSigma Cat# MCYTMAG-70K-PX32 IFNg ELISA assay BioLegend Cat# 430807 Single cell secretomes-human PhenomeX Cat# ISOCODE-1001-4 Single cell secretomes-mouse PhenomeX Cat# ISOCODE-1004-4 TruSeq RNA UD Indexes Illumina Cat# 20020591 TruSeq Stranded mRNA LP Illumina Cat# 20020595 CD4+ cell isolation Miltenyi Biotec Cat# 130-104-454 CD8+ cell isolation Miltenyi Biotec Cat# 130-104-075 NK cell isolation Miltenyi Biotec Cat# 130-115-818 Deposited data RNA seq This study GEO : GSE209945 Experimental models: Cell lines MOC2 (M2w) Brigham and Women’s Hospital and Dana-Farber Cancer Institute Dr. Ravindra Uppaluri MOC2-huEGFR (M2h) University of Wisconsin This study B16 Scripps Research Institute Dr. Ralph Reisfield B78 Scripps Research Institute Dr. Ralph Reisfield SCC7 La Jolla Institute Dr. Stephen Schoenberger Panc02 National Cancer institute This study Experimental models: Organisms/strains C57BL/6 Taconic B6-F IFNgtm1Ts Jackson Laboratory IMSR_JAX:002287 FOXP3tm3(DTR/GFP)Ayr Jackson Laboratory IMSR_JAX:016958 Klrk1tm1Dhr Jackson Laboratory IMSR_JAX:022733 Fcer1gtm1Rav Jackson Laboratory IMSR_JAX:002847 NOD-scid IL2Rgnull University of Wisconsin Dr. Paul Lambert Oligonucleotides Murine CD86 forward/reverse primers IDT N/A Murine HPRT forward/reverse primers IDT N/A Recombinant DNA CRISPR CD86 gRNA IDT Mm.Cas9.CD86.1.AA Software and algorithms FlowJo BD N/A (Continued on next page) 20 Cell Reports 42, 113556, December 26, 2023 ..



    Similar Products

    ulbp  (Proteintech)
    93
    Proteintech ulbp
    Ulbp, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mult-1/ULBP1+Antibody/pmc12823515-28-0-9
    Average 93 stars, based on 1 article reviews
    ulbp - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    anti mmult 1  (R&D Systems)
    93
    R&D Systems anti mmult 1
    Anti Mmult 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mult-1/Mouse+ULBP-1%2FMULT-1+Antibody/pmc12784537-57-15-16
    Average 93 stars, based on 1 article reviews
    anti mmult 1 - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    anti mult 1 pe  (R&D Systems)
    93
    R&D Systems anti mult 1 pe
    Anti Mult 1 Pe, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mult-1/Mouse+ULBP-1%2FMULT-1+Antibody/us12414968-1619-28-30
    Average 93 stars, based on 1 article reviews
    anti mult 1 pe - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    mult-1 antibody  (Millipore)
    90
    Millipore mult-1 antibody
    Mult 1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mult-1/mult+1+antibody/pmc12256444-67-7-13
    Average 90 stars, based on 1 article reviews
    mult-1 antibody - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    mult 1  (Proteintech)
    93
    Proteintech mult 1
    Mult 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mult-1/ULBP1+Antibody/pm39849761-166-77-81
    Average 93 stars, based on 1 article reviews
    mult 1 - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    mult1  (R&D Systems)
    93
    R&D Systems mult1
    Mult1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mult-1/Mouse+ULBP-1%2FMULT-1+PE-conjugated+Antibody/pm39199623-59-18-19
    Average 93 stars, based on 1 article reviews
    mult1 - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    anti ulbp1 mult1  (R&D Systems)
    93
    R&D Systems anti ulbp1 mult1
    Anti Ulbp1 Mult1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mult-1/Mouse+ULBP-1%2FMULT-1+Antibody/10__1158_slash_2159___8290__cd___23___1451-409-78-80
    Average 93 stars, based on 1 article reviews
    anti ulbp1 mult1 - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    pe  (R&D Systems)
    93
    R&D Systems pe
    Pe, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mult-1/Mouse+ULBP-1%2FMULT-1+PE-conjugated+Antibody/pmc11069534-209-63-67
    Average 93 stars, based on 1 article reviews
    pe - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    anti mrae 1 pan antibody  (R&D Systems)
    93
    R&D Systems anti mrae 1 pan antibody
    Anti Mrae 1 Pan Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mult-1/Mouse+ULBP-1%2FMULT-1+PE-conjugated+Antibody/pm38704364-183-50-61
    Average 93 stars, based on 1 article reviews
    anti mrae 1 pan antibody - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier

    Image Search Results