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mtp format  (Greiner Bio)


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    Greiner Bio mtp format
    Mtp Format, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtp+format/Microplate+96+Well+Pp+F-Bottom/pm40143794-75-6-8
    Average 96 stars, based on 328 article reviews
    mtp format - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Rescue of Cognitive Deficits in a Mouse Model of Alzheimer's Disease with a Novel Brominated P2 × 7 Receptor Antagonist.
    Article Snippet: P2 × 7 receptor (P2 × 7R) represents a promising therapeutic target for Alzheimer’s disease (AD), given its marked upregulation in neuroinflammation and involvement in amyloid-β (Aβ) and tau pathology.. Although several P2 × 7R antagonists with high central nervous system (CNS) penetration and cross-species activity have been developed, none have yet reached clinical use, underscoring the need for optimized agents suitable for chronic neurological conditions.. In this study, we designed a series of brominated P2 × 7R antagonists based on a prominent antagonist Lu AF27139, among which the lead compound YH1 exhibited favorable lipophilicity, brain penetration, plasma stability, and receptor binding.

    Incubation:

    Article Title: Local citrus sudden death-associated virus infection in Nicotiana benthamiana induces chloroplast structural changes and HR-like responses that do not restrict systemic virus movement.
    Article Snippet: .. Samples were vortexed, transferred to a black polypropylene 96- well plate (Greiner Bio- One, 655209) and incubated at RT in the dark for 20 min. .. Relative fluorescence intensity was measured using a FLUOstar OPTIMA microplate reader (BMG LABTECH) with excitation at 485 nm, emission at 520 nm and gain set to 1,800.

    Article Title: Sulfonyl benzamide derivatives as Bcl-2 inhibitors
    Article Snippet: .. Bcl-2 WT and Bcl-2 G101V Inhibition: Assay Conditions: Assay plate: Greiner Bio-One 655209, Black, flat bottom 96 well Plate Assay buffer: 100 mM potassium phosphate, pH 7.5; 100 μg/ml bovine gamma globulin; 0.02% sodium azide, plus 0.01% Triton X (added right before assay) Assay volume: 100 μL Final [DMSO]=2.5% (2.5 μL compound solution in DMSO plus 97.5 μL of protein/tracer mixture or tracer only solution in assay buffer) Incubation time: 1 hours Final Concentrations: [Bcl2]=20 nM, [Flu-BIM]=5 nM Procedures: Assay solution preparations: (a) Prepare the complex solution (Protein plus Flu-BIM) in following concentrations: 20.5 nM of Bcl2 protein and 5.1 nM Flu-BIM in the assay buffer; (b) Prepare the positive control which is the free Flu-BIM (5.1 nM) in the assay buffer. ..

    Inhibition:

    Article Title: Sulfonyl benzamide derivatives as Bcl-2 inhibitors
    Article Snippet: .. Bcl-2 WT and Bcl-2 G101V Inhibition: Assay Conditions: Assay plate: Greiner Bio-One 655209, Black, flat bottom 96 well Plate Assay buffer: 100 mM potassium phosphate, pH 7.5; 100 μg/ml bovine gamma globulin; 0.02% sodium azide, plus 0.01% Triton X (added right before assay) Assay volume: 100 μL Final [DMSO]=2.5% (2.5 μL compound solution in DMSO plus 97.5 μL of protein/tracer mixture or tracer only solution in assay buffer) Incubation time: 1 hours Final Concentrations: [Bcl2]=20 nM, [Flu-BIM]=5 nM Procedures: Assay solution preparations: (a) Prepare the complex solution (Protein plus Flu-BIM) in following concentrations: 20.5 nM of Bcl2 protein and 5.1 nM Flu-BIM in the assay buffer; (b) Prepare the positive control which is the free Flu-BIM (5.1 nM) in the assay buffer. ..

    Positive Control:

    Article Title: Sulfonyl benzamide derivatives as Bcl-2 inhibitors
    Article Snippet: .. Bcl-2 WT and Bcl-2 G101V Inhibition: Assay Conditions: Assay plate: Greiner Bio-One 655209, Black, flat bottom 96 well Plate Assay buffer: 100 mM potassium phosphate, pH 7.5; 100 μg/ml bovine gamma globulin; 0.02% sodium azide, plus 0.01% Triton X (added right before assay) Assay volume: 100 μL Final [DMSO]=2.5% (2.5 μL compound solution in DMSO plus 97.5 μL of protein/tracer mixture or tracer only solution in assay buffer) Incubation time: 1 hours Final Concentrations: [Bcl2]=20 nM, [Flu-BIM]=5 nM Procedures: Assay solution preparations: (a) Prepare the complex solution (Protein plus Flu-BIM) in following concentrations: 20.5 nM of Bcl2 protein and 5.1 nM Flu-BIM in the assay buffer; (b) Prepare the positive control which is the free Flu-BIM (5.1 nM) in the assay buffer. ..

    Suspension:

    Article Title: Large-scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation.
    Article Snippet: .. For each time point (0, 1, 3, 12, 24, 48, 72 h), 5 μL of fibril suspension was added to 195 μL ThT solution in a black-walled 96-well plate (Catalog number: 655209; Greiner Bio-One, Kremsmünster, Austria). .. After 5 min of incubation (25 °C), fluorescence was measured using a FLUOstar Omega Microplate Reader (BMG Labtech, Offenburg, Germany) at 450 nm excitation/480 nm emission.



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    Image Search Results


    T m data from a 96‐well MTP made up of 92 clones of the saturation library DERA Lb Q54X, two wild‐type controls (wt) and two negative controls (neg) lacking the gene coding for DERA Lb and thus not giving a corresponding melting curve. The red bars correspond to the T m values ±1 standard deviation from triplicate determinations. From the entire set 32 clones were sequenced, and these bars are labelled with the amino acid found at position 54. The high reproducibility of triplicate measurements, the good agreement of identical variants and the high yield of positively determined T m data demonstrate the high sample quality and the reliability of the method.

    Journal: The Febs Journal

    Article Title: nanoDSF as screening tool for enzyme libraries and biotechnology development

    doi: 10.1111/febs.14696

    Figure Lengend Snippet: T m data from a 96‐well MTP made up of 92 clones of the saturation library DERA Lb Q54X, two wild‐type controls (wt) and two negative controls (neg) lacking the gene coding for DERA Lb and thus not giving a corresponding melting curve. The red bars correspond to the T m values ±1 standard deviation from triplicate determinations. From the entire set 32 clones were sequenced, and these bars are labelled with the amino acid found at position 54. The high reproducibility of triplicate measurements, the good agreement of identical variants and the high yield of positively determined T m data demonstrate the high sample quality and the reliability of the method.

    Article Snippet: The phylogenetically diverse 2‐deoxyribose‐5‐phosphate (DERA) library was provided as lyophilised cell‐free extract in a commercial MTP format (Prozomix Limited, Haltwhistle, UK).

    Techniques: Clone Assay, Standard Deviation