Review



michael davidson  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc michael davidson
    Michael Davidson, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mruby2+n1+vector/mNeonGreen-mRuby2-FRET-10+(Plasmid+%2358179)/pmc12708940-70-14-16
    Average 93 stars, based on 5 article reviews
    michael davidson - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Construct:

    Article Title: Giardia intraflagellar transport protein 88 is involved in flagella formation
    Article Snippet: .. To make C-terminal mNG tagged construct, mNG gene was amplified from mNG mRuby2-FRET-10 plasmid (Addgene, Watertown, MA, USA, Cat. No. 58179) by PCR using 2 primers, N11-linker-FBamH1 and mNG-R-ER1 (Supplementary Table S2). ..

    Article Title: Giardia intraflagellar transport protein 88 is involved in flagella formation
    Article Snippet: .. To make C-terminal mNG tagged construct, mNG gene was amplified from mNG mRuby2-FRET-10 plasmid (Addgene, Watertown, MA, USA, Cat. No. 58179) by PCR using 2 primers, N11-linker-F-BamH1 and mNG-R-ER1 ( ). ..

    Article Title: Different mCherry isoforms show distinct photophysical properties in FRET tandem constructs
    Article Snippet: .. The mNeonGreen sequence was cut-off from a mNeonGreen-mRuby2-FRET-10 construct, which was a gift from Michael Davidson (Addgene plasmid # 58179; http://n2t.net/addgene:58179 ; RRID:Addgene_58179) , using Age I/ Not I and cloned into a pEGFP-N1 backbone (Clontech) without the EGFP coding sequence producing a mNeonGreen vector possessing common Multiple Cloning Sites (MCS). ..

    Article Title: A Practical Approach to Quantitatively Assessing Equilibrium-Constant Accuracy from a Single Binding Isotherm
    Article Snippet: .. Note S10: Plasmid construction and mutagenesis The plasmid for expression and purification of human HSP90α-mNeonGreen (HSP90α UniProtKB: P07900) was constructed in a previous work.11 The mNeonGreen (FPbase ID: ZRKRV) gene sequence from Branchiostoma lanceolatum was obtained from Addgene plasmid #58179 (Addgene HQ, NY, Watertown, USA).12 The DNA sequence of human CDC37 (UniProtKB: Q16543) was obtained from Addgene plasmid #163838. ..

    Amplification:

    Article Title: Giardia intraflagellar transport protein 88 is involved in flagella formation
    Article Snippet: .. To make C-terminal mNG tagged construct, mNG gene was amplified from mNG mRuby2-FRET-10 plasmid (Addgene, Watertown, MA, USA, Cat. No. 58179) by PCR using 2 primers, N11-linker-FBamH1 and mNG-R-ER1 (Supplementary Table S2). ..

    Article Title: Giardia intraflagellar transport protein 88 is involved in flagella formation
    Article Snippet: .. To make C-terminal mNG tagged construct, mNG gene was amplified from mNG mRuby2-FRET-10 plasmid (Addgene, Watertown, MA, USA, Cat. No. 58179) by PCR using 2 primers, N11-linker-F-BamH1 and mNG-R-ER1 ( ). ..

    Plasmid Preparation:

    Article Title: Giardia intraflagellar transport protein 88 is involved in flagella formation
    Article Snippet: .. To make C-terminal mNG tagged construct, mNG gene was amplified from mNG mRuby2-FRET-10 plasmid (Addgene, Watertown, MA, USA, Cat. No. 58179) by PCR using 2 primers, N11-linker-FBamH1 and mNG-R-ER1 (Supplementary Table S2). ..

    Article Title: Giardia intraflagellar transport protein 88 is involved in flagella formation
    Article Snippet: .. To make C-terminal mNG tagged construct, mNG gene was amplified from mNG mRuby2-FRET-10 plasmid (Addgene, Watertown, MA, USA, Cat. No. 58179) by PCR using 2 primers, N11-linker-F-BamH1 and mNG-R-ER1 ( ). ..

    Article Title: Different mCherry isoforms show distinct photophysical properties in FRET tandem constructs
    Article Snippet: .. The mNeonGreen sequence was cut-off from a mNeonGreen-mRuby2-FRET-10 construct, which was a gift from Michael Davidson (Addgene plasmid # 58179; http://n2t.net/addgene:58179 ; RRID:Addgene_58179) , using Age I/ Not I and cloned into a pEGFP-N1 backbone (Clontech) without the EGFP coding sequence producing a mNeonGreen vector possessing common Multiple Cloning Sites (MCS). ..

    Article Title: A Practical Approach to Quantitatively Assessing Equilibrium-Constant Accuracy from a Single Binding Isotherm
    Article Snippet: .. Note S10: Plasmid construction and mutagenesis The plasmid for expression and purification of human HSP90α-mNeonGreen (HSP90α UniProtKB: P07900) was constructed in a previous work.11 The mNeonGreen (FPbase ID: ZRKRV) gene sequence from Branchiostoma lanceolatum was obtained from Addgene plasmid #58179 (Addgene HQ, NY, Watertown, USA).12 The DNA sequence of human CDC37 (UniProtKB: Q16543) was obtained from Addgene plasmid #163838. ..

    Polymerase Chain Reaction:

    Article Title: Giardia intraflagellar transport protein 88 is involved in flagella formation
    Article Snippet: .. To make C-terminal mNG tagged construct, mNG gene was amplified from mNG mRuby2-FRET-10 plasmid (Addgene, Watertown, MA, USA, Cat. No. 58179) by PCR using 2 primers, N11-linker-FBamH1 and mNG-R-ER1 (Supplementary Table S2). ..

    Article Title: Giardia intraflagellar transport protein 88 is involved in flagella formation
    Article Snippet: .. To make C-terminal mNG tagged construct, mNG gene was amplified from mNG mRuby2-FRET-10 plasmid (Addgene, Watertown, MA, USA, Cat. No. 58179) by PCR using 2 primers, N11-linker-F-BamH1 and mNG-R-ER1 ( ). ..

    Sequencing:

    Article Title: Different mCherry isoforms show distinct photophysical properties in FRET tandem constructs
    Article Snippet: .. The mNeonGreen sequence was cut-off from a mNeonGreen-mRuby2-FRET-10 construct, which was a gift from Michael Davidson (Addgene plasmid # 58179; http://n2t.net/addgene:58179 ; RRID:Addgene_58179) , using Age I/ Not I and cloned into a pEGFP-N1 backbone (Clontech) without the EGFP coding sequence producing a mNeonGreen vector possessing common Multiple Cloning Sites (MCS). ..

    Article Title: A Practical Approach to Quantitatively Assessing Equilibrium-Constant Accuracy from a Single Binding Isotherm
    Article Snippet: .. Note S10: Plasmid construction and mutagenesis The plasmid for expression and purification of human HSP90α-mNeonGreen (HSP90α UniProtKB: P07900) was constructed in a previous work.11 The mNeonGreen (FPbase ID: ZRKRV) gene sequence from Branchiostoma lanceolatum was obtained from Addgene plasmid #58179 (Addgene HQ, NY, Watertown, USA).12 The DNA sequence of human CDC37 (UniProtKB: Q16543) was obtained from Addgene plasmid #163838. ..

    Clone Assay:

    Article Title: Different mCherry isoforms show distinct photophysical properties in FRET tandem constructs
    Article Snippet: .. The mNeonGreen sequence was cut-off from a mNeonGreen-mRuby2-FRET-10 construct, which was a gift from Michael Davidson (Addgene plasmid # 58179; http://n2t.net/addgene:58179 ; RRID:Addgene_58179) , using Age I/ Not I and cloned into a pEGFP-N1 backbone (Clontech) without the EGFP coding sequence producing a mNeonGreen vector possessing common Multiple Cloning Sites (MCS). ..

    Cloning:

    Article Title: Different mCherry isoforms show distinct photophysical properties in FRET tandem constructs
    Article Snippet: .. The mNeonGreen sequence was cut-off from a mNeonGreen-mRuby2-FRET-10 construct, which was a gift from Michael Davidson (Addgene plasmid # 58179; http://n2t.net/addgene:58179 ; RRID:Addgene_58179) , using Age I/ Not I and cloned into a pEGFP-N1 backbone (Clontech) without the EGFP coding sequence producing a mNeonGreen vector possessing common Multiple Cloning Sites (MCS). ..

    Mutagenesis:

    Article Title: A Practical Approach to Quantitatively Assessing Equilibrium-Constant Accuracy from a Single Binding Isotherm
    Article Snippet: .. Note S10: Plasmid construction and mutagenesis The plasmid for expression and purification of human HSP90α-mNeonGreen (HSP90α UniProtKB: P07900) was constructed in a previous work.11 The mNeonGreen (FPbase ID: ZRKRV) gene sequence from Branchiostoma lanceolatum was obtained from Addgene plasmid #58179 (Addgene HQ, NY, Watertown, USA).12 The DNA sequence of human CDC37 (UniProtKB: Q16543) was obtained from Addgene plasmid #163838. ..

    Expressing:

    Article Title: A Practical Approach to Quantitatively Assessing Equilibrium-Constant Accuracy from a Single Binding Isotherm
    Article Snippet: .. Note S10: Plasmid construction and mutagenesis The plasmid for expression and purification of human HSP90α-mNeonGreen (HSP90α UniProtKB: P07900) was constructed in a previous work.11 The mNeonGreen (FPbase ID: ZRKRV) gene sequence from Branchiostoma lanceolatum was obtained from Addgene plasmid #58179 (Addgene HQ, NY, Watertown, USA).12 The DNA sequence of human CDC37 (UniProtKB: Q16543) was obtained from Addgene plasmid #163838. ..

    Purification:

    Article Title: A Practical Approach to Quantitatively Assessing Equilibrium-Constant Accuracy from a Single Binding Isotherm
    Article Snippet: .. Note S10: Plasmid construction and mutagenesis The plasmid for expression and purification of human HSP90α-mNeonGreen (HSP90α UniProtKB: P07900) was constructed in a previous work.11 The mNeonGreen (FPbase ID: ZRKRV) gene sequence from Branchiostoma lanceolatum was obtained from Addgene plasmid #58179 (Addgene HQ, NY, Watertown, USA).12 The DNA sequence of human CDC37 (UniProtKB: Q16543) was obtained from Addgene plasmid #163838. ..



    Similar Products

    mruby2 n1 base vector  (Addgene inc)
    93
    Addgene inc mruby2 n1 base vector
    Mruby2 N1 Base Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mruby2+n1+vector/mRuby2-N1+(Plasmid+%2354614)/pmc09192689-494-18-21
    Average 93 stars, based on 1 article reviews
    mruby2 n1 base vector - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    mruby2 n1 vector  (Addgene inc)
    93
    Addgene inc mruby2 n1 vector
    Mruby2 N1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mruby2+n1+vector/mRuby2-N1+(Plasmid+%2354614)/pm33965400-40-47-49
    Average 93 stars, based on 1 article reviews
    mruby2 n1 vector - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    mclover2 c1 vector  (Addgene inc)
    93
    Addgene inc mclover2 c1 vector
    Mclover2 C1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mruby2+n1+vector/mRuby2-N1+(Plasmid+%2354614)/pm33965400-40-35-37
    Average 93 stars, based on 1 article reviews
    mclover2 c1 vector - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    mruby2 tubulin 6 vector  (Addgene inc)
    93
    Addgene inc mruby2 tubulin 6 vector
    CLASP2 and G2L1 colocalize in 3T3-L1 adipocytes. A–B, Live-cell imaging of adipocytes cultured in complete media coexpressing <t>mRuby2-Tubulin</t> (magenta) and GFP-CLASP2-HA (A, green) or mCherry-G2L1-myc (B, green). Live cells were imaged using TIRFM on a 2-s acquisition interval. Time series images to the right of the whole cell image are used to highlight +TIP dynamics within the indicated ROI. C, Immunofluorescence images of adipocytes cultured in complete media cooverexpressing GFP-CLASP2-HA (green) and mCherry-G2L1-myc (magenta). Cells were fixed and immunolabeled for tubulin (inverted white). Bottom row is magnified ROI. ROI, region of interest. Scale bar = 20 μm.
    Mruby2 Tubulin 6 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mruby2+n1+vector/piRFP670-N1+(Plasmid+%2345457)/pmc06601206-97-10-15
    Average 93 stars, based on 1 article reviews
    mruby2 tubulin 6 vector - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    CLASP2 and G2L1 colocalize in 3T3-L1 adipocytes. A–B, Live-cell imaging of adipocytes cultured in complete media coexpressing mRuby2-Tubulin (magenta) and GFP-CLASP2-HA (A, green) or mCherry-G2L1-myc (B, green). Live cells were imaged using TIRFM on a 2-s acquisition interval. Time series images to the right of the whole cell image are used to highlight +TIP dynamics within the indicated ROI. C, Immunofluorescence images of adipocytes cultured in complete media cooverexpressing GFP-CLASP2-HA (green) and mCherry-G2L1-myc (magenta). Cells were fixed and immunolabeled for tubulin (inverted white). Bottom row is magnified ROI. ROI, region of interest. Scale bar = 20 μm.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Insulin Induces Microtubule Stabilization and Regulates the Microtubule Plus-end Tracking Protein Network in Adipocytes *

    doi: 10.1074/mcp.RA119.001450

    Figure Lengend Snippet: CLASP2 and G2L1 colocalize in 3T3-L1 adipocytes. A–B, Live-cell imaging of adipocytes cultured in complete media coexpressing mRuby2-Tubulin (magenta) and GFP-CLASP2-HA (A, green) or mCherry-G2L1-myc (B, green). Live cells were imaged using TIRFM on a 2-s acquisition interval. Time series images to the right of the whole cell image are used to highlight +TIP dynamics within the indicated ROI. C, Immunofluorescence images of adipocytes cultured in complete media cooverexpressing GFP-CLASP2-HA (green) and mCherry-G2L1-myc (magenta). Cells were fixed and immunolabeled for tubulin (inverted white). Bottom row is magnified ROI. ROI, region of interest. Scale bar = 20 μm.

    Article Snippet: To generate pLenti-iRFP670-Tubulin, tubulin was first subcloned out of the mRuby2-Tubulin-6 vector and into piRFP670-N1 (Addgene #45457, a gift from Vladislav Verkhusha). iRFP670-Tubulin was then subcloned out of the piRFP670-Tubulin vector and into the pLenti backbone to generate pLenti-iRFP670-Tubulin.

    Techniques: Live Cell Imaging, Cell Culture, Immunofluorescence, Immunolabeling

    The effect of insulin on CLASP2 +TIP dynamics. Live-cell imaging of adipocytes serum-starved for one hour and subsequently stimulated with 100 nm insulin. Live cells were imaged using TIRFM on a two-second acquisition interval. A, Single frame of live-cell imaging of adipocytes coexpressing GFP-CLASP2 (green) and mRuby2-Tubulin (magenta) at basal state (top row) or 10 mins (bottom row) following insulin stimulation. CLASP2 is displayed in inverted white to highlight +TIP density. B, Quantification of CLASP2-containing +TIP density per unit area (μm2) in adipocytes at basal state or 10 mins following insulin stimulation. Percent increase in CLASP2-containing +TIP density is indicated to the right. Statistical comparison made by paired parametric t test, n = 5 cells. C, Temporally color-coded projection of GFP-CLASP2 localization during a 30 s live-cell imaging interval. The length and extent of color overlap of time-projected CLASP2 localization indicates reduced displacement and hence lower velocity of CLASP2-containing +TIPs during the imaging interval in adipocytes after 10 mins of insulin stimulation. D, Quantification of CLASP2-containing +TIP velocity in adipocytes at basal state or 10 mins following insulin stimulation indicates reduced velocity of CLASP2-containing +TIPS after insulin treatment. Statistical comparison made by unpaired parametric t test, n = 123–125 CLASP2-containing +TIPS from five cells. Scale bars = 5 μm. E, Image of entire cells in the basal state (left panel) or 8 mins post insulin treatment (right panel) extracted from supplemental Video 2. Time series images under the whole cell image are used to highlight insulin-stimulated +TIP dynamics within the indicated ROI. F, Time series extracted from supplemental Video 2 of the indicated ROIs at either the basal state or at 4 mins post insulin treatment to present an example of changing CLASP2 microtubule plus-end dynamics with insulin stimulation. G, Live cell CLASP2-containing +TIP dynamics of the ROI extracted from supplemental Video 2 were captured in the basal state followed by stimulation with insulin. Each insulin-stimulated CLASP2-containing +TIP trail length time point was compared against the basal CLASP2-containing +TIP trail length to test for significant differences. t test; *p ≤ 0.05, **p ≤ 0.01. Red circles represent outlier data points. ROI, region of interest. BAS, basal. INS, insulin. Scale bar = 10 μm.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Insulin Induces Microtubule Stabilization and Regulates the Microtubule Plus-end Tracking Protein Network in Adipocytes *

    doi: 10.1074/mcp.RA119.001450

    Figure Lengend Snippet: The effect of insulin on CLASP2 +TIP dynamics. Live-cell imaging of adipocytes serum-starved for one hour and subsequently stimulated with 100 nm insulin. Live cells were imaged using TIRFM on a two-second acquisition interval. A, Single frame of live-cell imaging of adipocytes coexpressing GFP-CLASP2 (green) and mRuby2-Tubulin (magenta) at basal state (top row) or 10 mins (bottom row) following insulin stimulation. CLASP2 is displayed in inverted white to highlight +TIP density. B, Quantification of CLASP2-containing +TIP density per unit area (μm2) in adipocytes at basal state or 10 mins following insulin stimulation. Percent increase in CLASP2-containing +TIP density is indicated to the right. Statistical comparison made by paired parametric t test, n = 5 cells. C, Temporally color-coded projection of GFP-CLASP2 localization during a 30 s live-cell imaging interval. The length and extent of color overlap of time-projected CLASP2 localization indicates reduced displacement and hence lower velocity of CLASP2-containing +TIPs during the imaging interval in adipocytes after 10 mins of insulin stimulation. D, Quantification of CLASP2-containing +TIP velocity in adipocytes at basal state or 10 mins following insulin stimulation indicates reduced velocity of CLASP2-containing +TIPS after insulin treatment. Statistical comparison made by unpaired parametric t test, n = 123–125 CLASP2-containing +TIPS from five cells. Scale bars = 5 μm. E, Image of entire cells in the basal state (left panel) or 8 mins post insulin treatment (right panel) extracted from supplemental Video 2. Time series images under the whole cell image are used to highlight insulin-stimulated +TIP dynamics within the indicated ROI. F, Time series extracted from supplemental Video 2 of the indicated ROIs at either the basal state or at 4 mins post insulin treatment to present an example of changing CLASP2 microtubule plus-end dynamics with insulin stimulation. G, Live cell CLASP2-containing +TIP dynamics of the ROI extracted from supplemental Video 2 were captured in the basal state followed by stimulation with insulin. Each insulin-stimulated CLASP2-containing +TIP trail length time point was compared against the basal CLASP2-containing +TIP trail length to test for significant differences. t test; *p ≤ 0.05, **p ≤ 0.01. Red circles represent outlier data points. ROI, region of interest. BAS, basal. INS, insulin. Scale bar = 10 μm.

    Article Snippet: To generate pLenti-iRFP670-Tubulin, tubulin was first subcloned out of the mRuby2-Tubulin-6 vector and into piRFP670-N1 (Addgene #45457, a gift from Vladislav Verkhusha). iRFP670-Tubulin was then subcloned out of the piRFP670-Tubulin vector and into the pLenti backbone to generate pLenti-iRFP670-Tubulin.

    Techniques: Live Cell Imaging, Imaging