mruby2 n1 base vector (Addgene inc)
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mruby2 n1 base vector
Mruby2 N1 Base Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+n1+vector/mRuby2-N1+(Plasmid+%2354614)/pmc09192689-494-18-21
Average 93 stars, based on 18 article reviews
Mruby2 N1 Base Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+n1+vector/mRuby2-N1+(Plasmid+%2354614)/pmc09192689-494-18-21
Average 93 stars, based on 18 article reviews
mruby2 n1 base vector - by Bioz Stars,
2026-09
93/100 stars
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Construct:Article Title: Systems and methods for determining a treatment course of action Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and Article Title: IL-24 intrinsically regulates Th17 cell pathogenicity in mice Article Snippet: The sequences corresponding to full-length or nonsecretable (truncated) versions of IL-24, i.e., IL-24 and IL-24t, were cloned into mammalian expression vector pIRES-eGFP (6029-1; Clontech). .. The fusion constructs of nonsecretable IL-24 (mRuby2-N1-IL-24t; sequence: 5′-ATG CAA GAG TTC CGA TTT GGG TCT TGC CAA GTG ACA GGG GTG GTT CTC CCA GAA CTG TGG GAG GCC TTC TGG ACT GTG AAG AAC ACT GTG CAA ACT CAG GAT GAC ATC ACA AGC ATC CGG CTG TTG AAG CCG CAG GTT CTG CGG AAT GTC TCG GGT GCT GAG AGC TGT TAC CTT GCC CAC AGC CTG CTG AAG TTC TAC TTG AAC ACT GTT TTC AAG AAC TAC CAC AGC AAA ATA GCC AAA TTC AAG GTC TTG AGG TCA TTC TCC ACT CTG GCC AAC AAC TTC ATA GTC ATC ATG TCA CAA CTA CAG CCC AGT AAG GAC AAT TCC ATG CTT CCC ATT AGT GAG AGT GCA CAC CAG CGG TTT TTG CTG TTC CGC AGA GCA TTC AAA CAG TTG GAT ACA GAA GTC GCT TTG GTG AAA GCC TTT GGG GAA GTG GAC ATT CTC CTG ACC TGG ATG CAG AAA TTC TAC CAT CTC-3′) or IFN-γ (mRuby2-N1-IFN-γt; sequence: 5′-ATG CAC GGC ACA GTC ATT GAA AGC CTA GAA AGT CTG AAT AAC TAT TTT AAC TCA AGT GGC ATA GAT GTG GAA GAA AAG AGT CTC TTC TTG GAT ATC TGG AGG AAC TGG CAA AAG GAT GGT GAC ATG AAA ATC CTG CAG AGC CAG ATT ATC TCT TTC TAC CTC AGA CTC TTT GAA GTC TTG AAA GAC AAT CAG GCC ATC AGC AAC AAC ATA AGC GTC ATT GAA TCA CAC CTG ATT ACT ACC TTC TTC AGC AAC AGC AAG GCG AAA AAG GAT GCA TTC ATG AGT ATT GCC AAG TTT GAG GTC AAC AAC CCA CAG GTC CAG CGC CAA GCA TTC AAT GAG CTC ATC CGA GTG GTC CAC CAG CTG TTG CCG GAA TCC AGC CTC AGG AAG CGG AAA AGG AGT CGC TGC-3′) were generated by subcloning into Generated:Article Title: Systems and methods for determining a treatment course of action Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and Article Title: IL-24 intrinsically regulates Th17 cell pathogenicity in mice Article Snippet: The sequences corresponding to full-length or nonsecretable (truncated) versions of IL-24, i.e., IL-24 and IL-24t, were cloned into mammalian expression vector pIRES-eGFP (6029-1; Clontech). .. The fusion constructs of nonsecretable IL-24 (mRuby2-N1-IL-24t; sequence: 5′-ATG CAA GAG TTC CGA TTT GGG TCT TGC CAA GTG ACA GGG GTG GTT CTC CCA GAA CTG TGG GAG GCC TTC TGG ACT GTG AAG AAC ACT GTG CAA ACT CAG GAT GAC ATC ACA AGC ATC CGG CTG TTG AAG CCG CAG GTT CTG CGG AAT GTC TCG GGT GCT GAG AGC TGT TAC CTT GCC CAC AGC CTG CTG AAG TTC TAC TTG AAC ACT GTT TTC AAG AAC TAC CAC AGC AAA ATA GCC AAA TTC AAG GTC TTG AGG TCA TTC TCC ACT CTG GCC AAC AAC TTC ATA GTC ATC ATG TCA CAA CTA CAG CCC AGT AAG GAC AAT TCC ATG CTT CCC ATT AGT GAG AGT GCA CAC CAG CGG TTT TTG CTG TTC CGC AGA GCA TTC AAA CAG TTG GAT ACA GAA GTC GCT TTG GTG AAA GCC TTT GGG GAA GTG GAC ATT CTC CTG ACC TGG ATG CAG AAA TTC TAC CAT CTC-3′) or IFN-γ (mRuby2-N1-IFN-γt; sequence: 5′-ATG CAC GGC ACA GTC ATT GAA AGC CTA GAA AGT CTG AAT AAC TAT TTT AAC TCA AGT GGC ATA GAT GTG GAA GAA AAG AGT CTC TTC TTG GAT ATC TGG AGG AAC TGG CAA AAG GAT GGT GAC ATG AAA ATC CTG CAG AGC CAG ATT ATC TCT TTC TAC CTC AGA CTC TTT GAA GTC TTG AAA GAC AAT CAG GCC ATC AGC AAC AAC ATA AGC GTC ATT GAA TCA CAC CTG ATT ACT ACC TTC TTC AGC AAC AGC AAG GCG AAA AAG GAT GCA TTC ATG AGT ATT GCC AAG TTT GAG GTC AAC AAC CCA CAG GTC CAG CGC CAA GCA TTC AAT GAG CTC ATC CGA GTG GTC CAC CAG CTG TTG CCG GAA TCC AGC CTC AGG AAG CGG AAA AGG AGT CGC TGC-3′) were generated by subcloning into Polymerase Chain Reaction:Article Title: Systems and methods for determining a treatment course of action Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and Amplification:Article Title: Systems and methods for determining a treatment course of action Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and Article Title: Hijacking of transcriptional condensates by endogenous retroviruses. Article Snippet: To knock in the degradation-sensitive FKBPF36V tag at the N-terminus of TRIM28, a repair template containing homology arms spanning upstream and downstream of the target site was cloned into a pUC19 vector (NEB) (Supplementary Fig. 2a). .. The repair template included a mRuby2 fluorescent protein sequence, P2A linker and the FKBP tag sequence (Supplementary Fig. 2a)25. Sequencing:Article Title: IL-24 intrinsically regulates Th17 cell pathogenicity in mice Article Snippet: The sequences corresponding to full-length or nonsecretable (truncated) versions of IL-24, i.e., IL-24 and IL-24t, were cloned into mammalian expression vector pIRES-eGFP (6029-1; Clontech). .. The fusion constructs of nonsecretable IL-24 (mRuby2-N1-IL-24t; sequence: 5′-ATG CAA GAG TTC CGA TTT GGG TCT TGC CAA GTG ACA GGG GTG GTT CTC CCA GAA CTG TGG GAG GCC TTC TGG ACT GTG AAG AAC ACT GTG CAA ACT CAG GAT GAC ATC ACA AGC ATC CGG CTG TTG AAG CCG CAG GTT CTG CGG AAT GTC TCG GGT GCT GAG AGC TGT TAC CTT GCC CAC AGC CTG CTG AAG TTC TAC TTG AAC ACT GTT TTC AAG AAC TAC CAC AGC AAA ATA GCC AAA TTC AAG GTC TTG AGG TCA TTC TCC ACT CTG GCC AAC AAC TTC ATA GTC ATC ATG TCA CAA CTA CAG CCC AGT AAG GAC AAT TCC ATG CTT CCC ATT AGT GAG AGT GCA CAC CAG CGG TTT TTG CTG TTC CGC AGA GCA TTC AAA CAG TTG GAT ACA GAA GTC GCT TTG GTG AAA GCC TTT GGG GAA GTG GAC ATT CTC CTG ACC TGG ATG CAG AAA TTC TAC CAT CTC-3′) or IFN-γ (mRuby2-N1-IFN-γt; sequence: 5′-ATG CAC GGC ACA GTC ATT GAA AGC CTA GAA AGT CTG AAT AAC TAT TTT AAC TCA AGT GGC ATA GAT GTG GAA GAA AAG AGT CTC TTC TTG GAT ATC TGG AGG AAC TGG CAA AAG GAT GGT GAC ATG AAA ATC CTG CAG AGC CAG ATT ATC TCT TTC TAC CTC AGA CTC TTT GAA GTC TTG AAA GAC AAT CAG GCC ATC AGC AAC AAC ATA AGC GTC ATT GAA TCA CAC CTG ATT ACT ACC TTC TTC AGC AAC AGC AAG GCG AAA AAG GAT GCA TTC ATG AGT ATT GCC AAG TTT GAG GTC AAC AAC CCA CAG GTC CAG CGC CAA GCA TTC AAT GAG CTC ATC CGA GTG GTC CAC CAG CTG TTG CCG GAA TCC AGC CTC AGG AAG CGG AAA AGG AGT CGC TGC-3′) were generated by subcloning into Article Title: Hijacking of transcriptional condensates by endogenous retroviruses. Article Snippet: To knock in the degradation-sensitive FKBPF36V tag at the N-terminus of TRIM28, a repair template containing homology arms spanning upstream and downstream of the target site was cloned into a pUC19 vector (NEB) (Supplementary Fig. 2a). .. The repair template included a mRuby2 fluorescent protein sequence, P2A linker and the FKBP tag sequence (Supplementary Fig. 2a)25. Countercurrent Chromatography:Article Title: IL-24 intrinsically regulates Th17 cell pathogenicity in mice Article Snippet: The sequences corresponding to full-length or nonsecretable (truncated) versions of IL-24, i.e., IL-24 and IL-24t, were cloned into mammalian expression vector pIRES-eGFP (6029-1; Clontech). .. The fusion constructs of nonsecretable IL-24 (mRuby2-N1-IL-24t; sequence: 5′-ATG CAA GAG TTC CGA TTT GGG TCT TGC CAA GTG ACA GGG GTG GTT CTC CCA GAA CTG TGG GAG GCC TTC TGG ACT GTG AAG AAC ACT GTG CAA ACT CAG GAT GAC ATC ACA AGC ATC CGG CTG TTG AAG CCG CAG GTT CTG CGG AAT GTC TCG GGT GCT GAG AGC TGT TAC CTT GCC CAC AGC CTG CTG AAG TTC TAC TTG AAC ACT GTT TTC AAG AAC TAC CAC AGC AAA ATA GCC AAA TTC AAG GTC TTG AGG TCA TTC TCC ACT CTG GCC AAC AAC TTC ATA GTC ATC ATG TCA CAA CTA CAG CCC AGT AAG GAC AAT TCC ATG CTT CCC ATT AGT GAG AGT GCA CAC CAG CGG TTT TTG CTG TTC CGC AGA GCA TTC AAA CAG TTG GAT ACA GAA GTC GCT TTG GTG AAA GCC TTT GGG GAA GTG GAC ATT CTC CTG ACC TGG ATG CAG AAA TTC TAC CAT CTC-3′) or IFN-γ (mRuby2-N1-IFN-γt; sequence: 5′-ATG CAC GGC ACA GTC ATT GAA AGC CTA GAA AGT CTG AAT AAC TAT TTT AAC TCA AGT GGC ATA GAT GTG GAA GAA AAG AGT CTC TTC TTG GAT ATC TGG AGG AAC TGG CAA AAG GAT GGT GAC ATG AAA ATC CTG CAG AGC CAG ATT ATC TCT TTC TAC CTC AGA CTC TTT GAA GTC TTG AAA GAC AAT CAG GCC ATC AGC AAC AAC ATA AGC GTC ATT GAA TCA CAC CTG ATT ACT ACC TTC TTC AGC AAC AGC AAG GCG AAA AAG GAT GCA TTC ATG AGT ATT GCC AAG TTT GAG GTC AAC AAC CCA CAG GTC CAG CGC CAA GCA TTC AAT GAG CTC ATC CGA GTG GTC CAC CAG CTG TTG CCG GAA TCC AGC CTC AGG AAG CGG AAA AGG AGT CGC TGC-3′) were generated by subcloning into Subcloning:Article Title: IL-24 intrinsically regulates Th17 cell pathogenicity in mice Article Snippet: The sequences corresponding to full-length or nonsecretable (truncated) versions of IL-24, i.e., IL-24 and IL-24t, were cloned into mammalian expression vector pIRES-eGFP (6029-1; Clontech). .. The fusion constructs of nonsecretable IL-24 (mRuby2-N1-IL-24t; sequence: 5′-ATG CAA GAG TTC CGA TTT GGG TCT TGC CAA GTG ACA GGG GTG GTT CTC CCA GAA CTG TGG GAG GCC TTC TGG ACT GTG AAG AAC ACT GTG CAA ACT CAG GAT GAC ATC ACA AGC ATC CGG CTG TTG AAG CCG CAG GTT CTG CGG AAT GTC TCG GGT GCT GAG AGC TGT TAC CTT GCC CAC AGC CTG CTG AAG TTC TAC TTG AAC ACT GTT TTC AAG AAC TAC CAC AGC AAA ATA GCC AAA TTC AAG GTC TTG AGG TCA TTC TCC ACT CTG GCC AAC AAC TTC ATA GTC ATC ATG TCA CAA CTA CAG CCC AGT AAG GAC AAT TCC ATG CTT CCC ATT AGT GAG AGT GCA CAC CAG CGG TTT TTG CTG TTC CGC AGA GCA TTC AAA CAG TTG GAT ACA GAA GTC GCT TTG GTG AAA GCC TTT GGG GAA GTG GAC ATT CTC CTG ACC TGG ATG CAG AAA TTC TAC CAT CTC-3′) or IFN-γ (mRuby2-N1-IFN-γt; sequence: 5′-ATG CAC GGC ACA GTC ATT GAA AGC CTA GAA AGT CTG AAT AAC TAT TTT AAC TCA AGT GGC ATA GAT GTG GAA GAA AAG AGT CTC TTC TTG GAT ATC TGG AGG AAC TGG CAA AAG GAT GGT GAC ATG AAA ATC CTG CAG AGC CAG ATT ATC TCT TTC TAC CTC AGA CTC TTT GAA GTC TTG AAA GAC AAT CAG GCC ATC AGC AAC AAC ATA AGC GTC ATT GAA TCA CAC CTG ATT ACT ACC TTC TTC AGC AAC AGC AAG GCG AAA AAG GAT GCA TTC ATG AGT ATT GCC AAG TTT GAG GTC AAC AAC CCA CAG GTC CAG CGC CAA GCA TTC AAT GAG CTC ATC CGA GTG GTC CAC CAG CTG TTG CCG GAA TCC AGC CTC AGG AAG CGG AAA AGG AGT CGC TGC-3′) were generated by subcloning into Transfection:Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma. Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer’s protocol. .. The Y79 cells were transfected with Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer's protocol. .. The Y79 cells were transfected with Plasmid Preparation:Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma. Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer’s protocol. .. The Y79 cells were transfected with Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer's protocol. .. The Y79 cells were transfected with Article Title: Hijacking of transcriptional condensates by endogenous retroviruses. Article Snippet: To knock in the degradation-sensitive FKBPF36V tag at the N-terminus of TRIM28, a repair template containing homology arms spanning upstream and downstream of the target site was cloned into a pUC19 vector (NEB) (Supplementary Fig. 2a). .. The repair template included a mRuby2 fluorescent protein sequence, P2A linker and the FKBP tag sequence (Supplementary Fig. 2a)25. Over Expression:Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma. Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer’s protocol. .. The Y79 cells were transfected with Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer's protocol. .. The Y79 cells were transfected with other:Article Title: Hijacking of transcriptional condensates by endogenous retroviruses. Article Snippet: A guide RNA (Supplementary Table 1b) targeting the N-terminus of TRIM28 was cloned into the |
