cerulean3 n1 (Addgene inc)
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cerulean3 n1
Cerulean3 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+n1+vector/Cerulean-N1+(Plasmid+%2354742)/pmc05414954-34-42-45
Average 92 stars, based on 14 article reviews
Cerulean3 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+n1+vector/Cerulean-N1+(Plasmid+%2354742)/pmc05414954-34-42-45
Average 92 stars, based on 14 article reviews
cerulean3 n1 - by Bioz Stars,
2026-10
92/100 stars
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Expressing:Article Title: Lack of CD8 + T cell effector differentiation during priming mediates checkpoint blockade resistance in non–small cell lung cancer Article Snippet: .. Generation of expression vectors The pLV-EF1a-IRES-puro vector (Addgene no. 85132) was used in conjunction with the cerulean-SIY insert generated using the Plasmid Preparation:Article Title: Lack of CD8 + T cell effector differentiation during priming mediates checkpoint blockade resistance in non–small cell lung cancer Article Snippet: .. Generation of expression vectors The pLV-EF1a-IRES-puro vector (Addgene no. 85132) was used in conjunction with the cerulean-SIY insert generated using the Article Title: Cytoplasmic localization of Mdm2 in cells expressing mutated NPM is mediated by p53. Article Snippet: .. NM_002392.6 from NCBI database) were PCR-amplified from plasmid MDM2p-Mdm2YFP (Plasmid # 53962; Addgene [60]) using extended primers and subsequently they were subcloned to vectors CeruleanN1 and Article Title: The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes Article Snippet: .. Upon ligation, the final open reading frame was confirmed through sequencing analysis to be as follows with TMP21 (rat) signal sequence and TMP21 (rat) ORF in all caps, and the linkers and CER in all lowercase: ATG TCT GGT TTG TCT GGC CCA CCA GCC CGG CGC GGC CCT TTT CCG TTA GCG TTG CTG CTT TTG TTC CTG CTC GGC CCC AGA TTG GTC CTT GCC cta ccg gtc gcc acc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag ctg atc tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc ctg ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac aac tac aac agc cac aac gtc tat atc acc gcc gac aag cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac atc gag gac ggc ggc gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac tac ctg agc tac cag tcc aag ctg agc aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac aag tcc gga ctc aga tct cga gct caa gct tcg AAT TCT ATC TCC TTC CAT CTA CCC GTG AAC TCT CGG AAG TGT CTC CGC GAG GAG ATC CAC AAA GAC TTG CTG GTT ACG GGC GCG TAC GAG ATC ACC GAC CAG TCT GGG GGC GCT GGC GGC CTG CGC ACC CAC CTC AAG ATC ACA GAT TCT GCT GGC CAT ATT CTG TAT GCC AAA GAG GAT GCA ACT AAA GGG AAG TTT GCC TTT ACC ACA GAA GAC TAT GAC ATG TTT GAA GTA TGC TTT GAG AGC AAG GGA ACA GGG CGG ATA CCT GAC CAA CTC GTG ATT CTA GAC ATG AAG CAT GGA GTA GAG GCG AAA AAT TAT GAA GAG ATC GCA AAA GTT GAG AAA CTC AAA CCA CTG GAG GTG GAG CTA CGG CGG CTC GAG GAC CTT TCA GAG TCT ATT GTT AAC GAC TTT GCC TAC ATG AAG AAG CGG GAA GAG GAG ATG AGG GAC ACC AAC GAG TCC ACA AAC ACC CGC GTC CTG TAC TTC AGC ATC TTT TCC ATG TTC TGC CTC ATT GGA CTA GCC ACC TGG CAG GTC TTC TAC CTG CGT CGC TTC TTC AAG GCC AAG AAG TTG ATA GAG TAA ATZ-VEN, AAT-VEN and AAT-CER: Starting with AAT-GFP, described previously (Granell et al., 2008), which was generously gifted by Giulia Baldini, we PCR amplified the AAT cDNA with primers encoding the BamHI and EcoRI cut sites, digested and inserted it into the pVenus-N1 (Addgene plasmid # 54640, gifted by Mike Davidson) or Article Title: Type I interferon activates MHC class I-dressed CD11b + conventional dendritic cells to promote protective anti-tumor CD8 + T cell immunity. Article Snippet: In brief Tumor-infiltrating dendritic cells (DCs) are central to the anti-tumor immune response.. Duong et al. reveal an activation state of CD11b conventional DCs (DC2) characterized by expression of interferon (IFN)-stimulated genes (ISG DCs) and capable of acquiring and presenting intact tumor-derived peptideMHC class I complexes.. ISG DCs can activate CD8 T cells and promote protective anti-tumor immunity in the absence of DC1. Article Title: Lack of CD8 + T cell effector differentiation during priming mediates checkpoint blockade resistance in non–small cell lung cancer Article Snippet: .. The pLV-EF1a-IRES-puro vector (Addgene no. 85132) was used in conjunction with the cerulean-SIY insert generated using the Generated:Article Title: Lack of CD8 + T cell effector differentiation during priming mediates checkpoint blockade resistance in non–small cell lung cancer Article Snippet: .. Generation of expression vectors The pLV-EF1a-IRES-puro vector (Addgene no. 85132) was used in conjunction with the cerulean-SIY insert generated using the Article Title: Type I interferon activates MHC class I-dressed CD11b + conventional dendritic cells to promote protective anti-tumor CD8 + T cell immunity. Article Snippet: In brief Tumor-infiltrating dendritic cells (DCs) are central to the anti-tumor immune response.. Duong et al. reveal an activation state of CD11b conventional DCs (DC2) characterized by expression of interferon (IFN)-stimulated genes (ISG DCs) and capable of acquiring and presenting intact tumor-derived peptideMHC class I complexes.. ISG DCs can activate CD8 T cells and promote protective anti-tumor immunity in the absence of DC1. Article Title: Lack of CD8 + T cell effector differentiation during priming mediates checkpoint blockade resistance in non–small cell lung cancer Article Snippet: .. The pLV-EF1a-IRES-puro vector (Addgene no. 85132) was used in conjunction with the cerulean-SIY insert generated using the other:Article Title: Compositions and methods for using cross-dressing to enhance anti-tumor immune responses Article Snippet: The insert was then cloned into the linearized pLV-EF1α-IRES-puro vector (final construct referred to as ‘pLV-EF1α-cerulean-SIIN—SIY-IRES-puro’) using the In-Fusion cloning kit (Takara Bio), amplified, and sequenced for accuracy. Polymerase Chain Reaction:Article Title: Cytoplasmic localization of Mdm2 in cells expressing mutated NPM is mediated by p53. Article Snippet: .. NM_002392.6 from NCBI database) were PCR-amplified from plasmid MDM2p-Mdm2YFP (Plasmid # 53962; Addgene [60]) using extended primers and subsequently they were subcloned to vectors CeruleanN1 and Article Title: The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes Article Snippet: .. Upon ligation, the final open reading frame was confirmed through sequencing analysis to be as follows with TMP21 (rat) signal sequence and TMP21 (rat) ORF in all caps, and the linkers and CER in all lowercase: ATG TCT GGT TTG TCT GGC CCA CCA GCC CGG CGC GGC CCT TTT CCG TTA GCG TTG CTG CTT TTG TTC CTG CTC GGC CCC AGA TTG GTC CTT GCC cta ccg gtc gcc acc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag ctg atc tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc ctg ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac aac tac aac agc cac aac gtc tat atc acc gcc gac aag cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac atc gag gac ggc ggc gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac tac ctg agc tac cag tcc aag ctg agc aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac aag tcc gga ctc aga tct cga gct caa gct tcg AAT TCT ATC TCC TTC CAT CTA CCC GTG AAC TCT CGG AAG TGT CTC CGC GAG GAG ATC CAC AAA GAC TTG CTG GTT ACG GGC GCG TAC GAG ATC ACC GAC CAG TCT GGG GGC GCT GGC GGC CTG CGC ACC CAC CTC AAG ATC ACA GAT TCT GCT GGC CAT ATT CTG TAT GCC AAA GAG GAT GCA ACT AAA GGG AAG TTT GCC TTT ACC ACA GAA GAC TAT GAC ATG TTT GAA GTA TGC TTT GAG AGC AAG GGA ACA GGG CGG ATA CCT GAC CAA CTC GTG ATT CTA GAC ATG AAG CAT GGA GTA GAG GCG AAA AAT TAT GAA GAG ATC GCA AAA GTT GAG AAA CTC AAA CCA CTG GAG GTG GAG CTA CGG CGG CTC GAG GAC CTT TCA GAG TCT ATT GTT AAC GAC TTT GCC TAC ATG AAG AAG CGG GAA GAG GAG ATG AGG GAC ACC AAC GAG TCC ACA AAC ACC CGC GTC CTG TAC TTC AGC ATC TTT TCC ATG TTC TGC CTC ATT GGA CTA GCC ACC TGG CAG GTC TTC TAC CTG CGT CGC TTC TTC AAG GCC AAG AAG TTG ATA GAG TAA ATZ-VEN, AAT-VEN and AAT-CER: Starting with AAT-GFP, described previously (Granell et al., 2008), which was generously gifted by Giulia Baldini, we PCR amplified the AAT cDNA with primers encoding the BamHI and EcoRI cut sites, digested and inserted it into the pVenus-N1 (Addgene plasmid # 54640, gifted by Mike Davidson) or Ligation:Article Title: The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes Article Snippet: .. Upon ligation, the final open reading frame was confirmed through sequencing analysis to be as follows with TMP21 (rat) signal sequence and TMP21 (rat) ORF in all caps, and the linkers and CER in all lowercase: ATG TCT GGT TTG TCT GGC CCA CCA GCC CGG CGC GGC CCT TTT CCG TTA GCG TTG CTG CTT TTG TTC CTG CTC GGC CCC AGA TTG GTC CTT GCC cta ccg gtc gcc acc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag ctg atc tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc ctg ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac aac tac aac agc cac aac gtc tat atc acc gcc gac aag cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac atc gag gac ggc ggc gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac tac ctg agc tac cag tcc aag ctg agc aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac aag tcc gga ctc aga tct cga gct caa gct tcg AAT TCT ATC TCC TTC CAT CTA CCC GTG AAC TCT CGG AAG TGT CTC CGC GAG GAG ATC CAC AAA GAC TTG CTG GTT ACG GGC GCG TAC GAG ATC ACC GAC CAG TCT GGG GGC GCT GGC GGC CTG CGC ACC CAC CTC AAG ATC ACA GAT TCT GCT GGC CAT ATT CTG TAT GCC AAA GAG GAT GCA ACT AAA GGG AAG TTT GCC TTT ACC ACA GAA GAC TAT GAC ATG TTT GAA GTA TGC TTT GAG AGC AAG GGA ACA GGG CGG ATA CCT GAC CAA CTC GTG ATT CTA GAC ATG AAG CAT GGA GTA GAG GCG AAA AAT TAT GAA GAG ATC GCA AAA GTT GAG AAA CTC AAA CCA CTG GAG GTG GAG CTA CGG CGG CTC GAG GAC CTT TCA GAG TCT ATT GTT AAC GAC TTT GCC TAC ATG AAG AAG CGG GAA GAG GAG ATG AGG GAC ACC AAC GAG TCC ACA AAC ACC CGC GTC CTG TAC TTC AGC ATC TTT TCC ATG TTC TGC CTC ATT GGA CTA GCC ACC TGG CAG GTC TTC TAC CTG CGT CGC TTC TTC AAG GCC AAG AAG TTG ATA GAG TAA ATZ-VEN, AAT-VEN and AAT-CER: Starting with AAT-GFP, described previously (Granell et al., 2008), which was generously gifted by Giulia Baldini, we PCR amplified the AAT cDNA with primers encoding the BamHI and EcoRI cut sites, digested and inserted it into the pVenus-N1 (Addgene plasmid # 54640, gifted by Mike Davidson) or Sequencing:Article Title: The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes Article Snippet: .. Upon ligation, the final open reading frame was confirmed through sequencing analysis to be as follows with TMP21 (rat) signal sequence and TMP21 (rat) ORF in all caps, and the linkers and CER in all lowercase: ATG TCT GGT TTG TCT GGC CCA CCA GCC CGG CGC GGC CCT TTT CCG TTA GCG TTG CTG CTT TTG TTC CTG CTC GGC CCC AGA TTG GTC CTT GCC cta ccg gtc gcc acc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag ctg atc tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc ctg ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac aac tac aac agc cac aac gtc tat atc acc gcc gac aag cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac atc gag gac ggc ggc gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac tac ctg agc tac cag tcc aag ctg agc aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac aag tcc gga ctc aga tct cga gct caa gct tcg AAT TCT ATC TCC TTC CAT CTA CCC GTG AAC TCT CGG AAG TGT CTC CGC GAG GAG ATC CAC AAA GAC TTG CTG GTT ACG GGC GCG TAC GAG ATC ACC GAC CAG TCT GGG GGC GCT GGC GGC CTG CGC ACC CAC CTC AAG ATC ACA GAT TCT GCT GGC CAT ATT CTG TAT GCC AAA GAG GAT GCA ACT AAA GGG AAG TTT GCC TTT ACC ACA GAA GAC TAT GAC ATG TTT GAA GTA TGC TTT GAG AGC AAG GGA ACA GGG CGG ATA CCT GAC CAA CTC GTG ATT CTA GAC ATG AAG CAT GGA GTA GAG GCG AAA AAT TAT GAA GAG ATC GCA AAA GTT GAG AAA CTC AAA CCA CTG GAG GTG GAG CTA CGG CGG CTC GAG GAC CTT TCA GAG TCT ATT GTT AAC GAC TTT GCC TAC ATG AAG AAG CGG GAA GAG GAG ATG AGG GAC ACC AAC GAG TCC ACA AAC ACC CGC GTC CTG TAC TTC AGC ATC TTT TCC ATG TTC TGC CTC ATT GGA CTA GCC ACC TGG CAG GTC TTC TAC CTG CGT CGC TTC TTC AAG GCC AAG AAG TTG ATA GAG TAA ATZ-VEN, AAT-VEN and AAT-CER: Starting with AAT-GFP, described previously (Granell et al., 2008), which was generously gifted by Giulia Baldini, we PCR amplified the AAT cDNA with primers encoding the BamHI and EcoRI cut sites, digested and inserted it into the pVenus-N1 (Addgene plasmid # 54640, gifted by Mike Davidson) or Article Title: Type I interferon activates MHC class I-dressed CD11b + conventional dendritic cells to promote protective anti-tumor CD8 + T cell immunity. Article Snippet: In brief Tumor-infiltrating dendritic cells (DCs) are central to the anti-tumor immune response.. Duong et al. reveal an activation state of CD11b conventional DCs (DC2) characterized by expression of interferon (IFN)-stimulated genes (ISG DCs) and capable of acquiring and presenting intact tumor-derived peptideMHC class I complexes.. ISG DCs can activate CD8 T cells and promote protective anti-tumor immunity in the absence of DC1. Countercurrent Chromatography:Article Title: The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes Article Snippet: .. Upon ligation, the final open reading frame was confirmed through sequencing analysis to be as follows with TMP21 (rat) signal sequence and TMP21 (rat) ORF in all caps, and the linkers and CER in all lowercase: ATG TCT GGT TTG TCT GGC CCA CCA GCC CGG CGC GGC CCT TTT CCG TTA GCG TTG CTG CTT TTG TTC CTG CTC GGC CCC AGA TTG GTC CTT GCC cta ccg gtc gcc acc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag ctg atc tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc ctg ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac aac tac aac agc cac aac gtc tat atc acc gcc gac aag cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac atc gag gac ggc ggc gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac tac ctg agc tac cag tcc aag ctg agc aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac aag tcc gga ctc aga tct cga gct caa gct tcg AAT TCT ATC TCC TTC CAT CTA CCC GTG AAC TCT CGG AAG TGT CTC CGC GAG GAG ATC CAC AAA GAC TTG CTG GTT ACG GGC GCG TAC GAG ATC ACC GAC CAG TCT GGG GGC GCT GGC GGC CTG CGC ACC CAC CTC AAG ATC ACA GAT TCT GCT GGC CAT ATT CTG TAT GCC AAA GAG GAT GCA ACT AAA GGG AAG TTT GCC TTT ACC ACA GAA GAC TAT GAC ATG TTT GAA GTA TGC TTT GAG AGC AAG GGA ACA GGG CGG ATA CCT GAC CAA CTC GTG ATT CTA GAC ATG AAG CAT GGA GTA GAG GCG AAA AAT TAT GAA GAG ATC GCA AAA GTT GAG AAA CTC AAA CCA CTG GAG GTG GAG CTA CGG CGG CTC GAG GAC CTT TCA GAG TCT ATT GTT AAC GAC TTT GCC TAC ATG AAG AAG CGG GAA GAG GAG ATG AGG GAC ACC AAC GAG TCC ACA AAC ACC CGC GTC CTG TAC TTC AGC ATC TTT TCC ATG TTC TGC CTC ATT GGA CTA GCC ACC TGG CAG GTC TTC TAC CTG CGT CGC TTC TTC AAG GCC AAG AAG TTG ATA GAG TAA ATZ-VEN, AAT-VEN and AAT-CER: Starting with AAT-GFP, described previously (Granell et al., 2008), which was generously gifted by Giulia Baldini, we PCR amplified the AAT cDNA with primers encoding the BamHI and EcoRI cut sites, digested and inserted it into the pVenus-N1 (Addgene plasmid # 54640, gifted by Mike Davidson) or Amplification:Article Title: The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes Article Snippet: .. Upon ligation, the final open reading frame was confirmed through sequencing analysis to be as follows with TMP21 (rat) signal sequence and TMP21 (rat) ORF in all caps, and the linkers and CER in all lowercase: ATG TCT GGT TTG TCT GGC CCA CCA GCC CGG CGC GGC CCT TTT CCG TTA GCG TTG CTG CTT TTG TTC CTG CTC GGC CCC AGA TTG GTC CTT GCC cta ccg gtc gcc acc atg gtg agc aag ggc gag gag ctg ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac ggc cac aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc tac ggc aag ctg acc ctg aag ctg atc tgc acc acc ggc aag ctg ccc gtg ccc tgg ccc acc ctc gtg acc acc ctg ggc tac ggc ctg cag tgc ttc gcc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac ggc aac tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg gtg aac cgc atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac atc ctg ggg cac aag ctg gag tac aac tac aac agc cac aac gtc tat atc acc gcc gac aag cag aag aac ggc atc aag gcc aac ttc aag atc cgc cac aac atc gag gac ggc ggc gtg cag ctc gcc gac cac tac cag cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac tac ctg agc tac cag tcc aag ctg agc aaa gac ccc aac gag aag cgc gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc ggc atg gac gag ctg tac aag tcc gga ctc aga tct cga gct caa gct tcg AAT TCT ATC TCC TTC CAT CTA CCC GTG AAC TCT CGG AAG TGT CTC CGC GAG GAG ATC CAC AAA GAC TTG CTG GTT ACG GGC GCG TAC GAG ATC ACC GAC CAG TCT GGG GGC GCT GGC GGC CTG CGC ACC CAC CTC AAG ATC ACA GAT TCT GCT GGC CAT ATT CTG TAT GCC AAA GAG GAT GCA ACT AAA GGG AAG TTT GCC TTT ACC ACA GAA GAC TAT GAC ATG TTT GAA GTA TGC TTT GAG AGC AAG GGA ACA GGG CGG ATA CCT GAC CAA CTC GTG ATT CTA GAC ATG AAG CAT GGA GTA GAG GCG AAA AAT TAT GAA GAG ATC GCA AAA GTT GAG AAA CTC AAA CCA CTG GAG GTG GAG CTA CGG CGG CTC GAG GAC CTT TCA GAG TCT ATT GTT AAC GAC TTT GCC TAC ATG AAG AAG CGG GAA GAG GAG ATG AGG GAC ACC AAC GAG TCC ACA AAC ACC CGC GTC CTG TAC TTC AGC ATC TTT TCC ATG TTC TGC CTC ATT GGA CTA GCC ACC TGG CAG GTC TTC TAC CTG CGT CGC TTC TTC AAG GCC AAG AAG TTG ATA GAG TAA ATZ-VEN, AAT-VEN and AAT-CER: Starting with AAT-GFP, described previously (Granell et al., 2008), which was generously gifted by Giulia Baldini, we PCR amplified the AAT cDNA with primers encoding the BamHI and EcoRI cut sites, digested and inserted it into the pVenus-N1 (Addgene plasmid # 54640, gifted by Mike Davidson) or Recombinant:Article Title: Type I interferon activates MHC class I-dressed CD11b + conventional dendritic cells to promote protective anti-tumor CD8 + T cell immunity. Article Snippet: In brief Tumor-infiltrating dendritic cells (DCs) are central to the anti-tumor immune response.. Duong et al. reveal an activation state of CD11b conventional DCs (DC2) characterized by expression of interferon (IFN)-stimulated genes (ISG DCs) and capable of acquiring and presenting intact tumor-derived peptideMHC class I complexes.. ISG DCs can activate CD8 T cells and promote protective anti-tumor immunity in the absence of DC1. Software:Article Title: Type I interferon activates MHC class I-dressed CD11b + conventional dendritic cells to promote protective anti-tumor CD8 + T cell immunity. Article Snippet: In brief Tumor-infiltrating dendritic cells (DCs) are central to the anti-tumor immune response.. Duong et al. reveal an activation state of CD11b conventional DCs (DC2) characterized by expression of interferon (IFN)-stimulated genes (ISG DCs) and capable of acquiring and presenting intact tumor-derived peptideMHC class I complexes.. ISG DCs can activate CD8 T cells and promote protective anti-tumor immunity in the absence of DC1. |
