gene expression microarray analyses of global mrna and risc-immunoprecipitated mrna in primary human astrocytes and u-87 astrocytoma cells (LC Sciences)
90
Structured Review
LC Sciences
gene expression microarray analyses of global mrna and risc-immunoprecipitated mrna in primary human astrocytes and u-87 astrocytoma cells
Gene Expression Microarray Analyses Of Global Mrna And Risc Immunoprecipitated Mrna In Primary Human Astrocytes And U 87 Astrocytoma Cells, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrna+expression+microarray+data/pmc02956662-133-9-21?v=LC+Sciences
Average 90 stars, based on 1 article reviews
Gene Expression Microarray Analyses Of Global Mrna And Risc Immunoprecipitated Mrna In Primary Human Astrocytes And U 87 Astrocytoma Cells, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrna+expression+microarray+data/pmc02956662-133-9-21?v=LC+Sciences
Average 90 stars, based on 1 article reviews
gene expression microarray analyses of global mrna and risc-immunoprecipitated mrna in primary human astrocytes and u-87 astrocytoma cells - by Bioz Stars,
2026-07
90/100 stars
Images
1) Product Images from "The MicroRNA and MessengerRNA Profile of the RNA-Induced Silencing Complex in Human Primary Astrocyte and Astrocytoma Cells"
Article Title: The MicroRNA and MessengerRNA Profile of the RNA-Induced Silencing Complex in Human Primary Astrocyte and Astrocytoma Cells
Journal: PLoS ONE
doi: 10.1371/journal.pone.0013445
Figure Legend Snippet: A hierarchical heatmap comparing global mRNA levels to RISC-IP mRNA levels in U-87 astrocytoma and primary astrocytes. MRNAs included in the heatmap had a fold change >1.4 and were significantly expressed (p<0.01).
Techniques Used:
Figure Legend Snippet: RISC-immunoprecipitated mRNA compared to global cellular mRNA in U-87 astrocytoma cells and primary astrocytes with a fold change > ±1.8 (p <0.01).
Techniques Used: RNA Binding Assay, Sequencing
Figure Legend Snippet: ( A ) MRNA microarray validation with qRT-PCR analysis in grouped RISC-IP U-87 astrocytoma and primary astrocytes samples. Grouped RISC-IP data were compared to the grouped global mRNA from U-87 astrocytoma and primary astrocytes samples. Eight mRNAs were selected from the grouped mRNA microarray dataset and examined by qRT-PCR. Fold change from the mRNA microarray are given by log2 values (left y-axis, light grey bars). Fold change from the qRT-PCR was determined using the 2 -ΔΔCt method and all mRNA expression values were normalized to the beta-actin endogenous control (right y-axis, dark grey bars). Error bars represent the standard deviation of the mean (SD). Importantly, the fold change (y-axis) cannot be directly compared between assays due to differences in calculation methods, but the general trend of up-regulation and down-regulation can be compared. ( B ) MRNAs in RISC compared to the global cellular milieu in U-87 astrocytoma cells. MRNA expression in U-87 astrocytoma cells were normalized to primary astrocytes mRNA expression. All mRNAs had a fold change >2.5 and were significantly expressed (p<0.01). Green and red arrows indicate decreased and increased levels respectively.
Techniques Used: Microarray, Quantitative RT-PCR, Expressing, Standard Deviation
Figure Legend Snippet: RISC-immunoprecipitated mRNA in human U-87 astrocytoma cells compared to RISC-immunoprecipitated mRNA in primary human astrocytes with a fold change >±2.6 (p <0.01).
Techniques Used:
Figure Legend Snippet: Bar charts display the relative number (-log(p-value)) of mRNAs with a fold change >2.5 and were considered significant (p<0.01). RISC-IP mRNA were indicated with dark blue bars and the global mRNA were indicated with light blue bars. The threshold (yellow lines) were set at p<0.01 and were calculated using Fischer's exact p-value test using IPA software.
Techniques Used: Software
Figure Legend Snippet: Bar charts display the relative number (-log(p-value)) of mRNAs with a fold change >2.5 and were considered significant (p<0.01). RISC-IP mRNA were indicated with dark blue bars and the global mRNA were indicated with light blue bars. The threshold (yellow lines) were set at p<0.01 and were calculated using Fischer's exact p-value test using IPA software.
Techniques Used: Software
Figure Legend Snippet: Specific messenger RNA fold change linked to the increased levels of miR-34a in U-87 astrocytoma RISC.
Techniques Used: Permeability, Migration, Expressing, Binding Assay, Isolation
Figure Legend Snippet: Specific messenger RNA fold change linked to increased levels of miR-195 in U-87 astrocytoma RISC.
Techniques Used: Transduction
