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procedure  (Bio-Rad)


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  • 99

    Structured Review

    Bio-Rad procedure
    Procedure, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 11377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mps+method/ChemiDoc+MP+System/pm40447301-55-8-12
    Average 99 stars, based on 11377 article reviews
    procedure - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Astroviruses use mucus secretion as a novel form of viral egress.
    Article Snippet: Membranes were blocked with 2.5% BSA for 1 hour at room temperature and then incubated with HAstV1 spike rabbit anti-sera (1:500, kind gift from Dr. Rebecca DuBois, University of California, Santa Cruz) or blocked with 5% milk and then incubated with FCGBP rabbit polyclonal antibody (1:500, Cloud-Clone Corp) overnight at 4°C. .. Membranes were washed three times for 5 minutes each in TBST, then incubated with anti-rabbit IgG HRP (1:1,000, Cell Signaling) for 1 hour at room temperature followed by Pierce ECL Western Blotting Substrate for HAstV1 capsid or Pierce SuperSignal West Pico PLUS Chemiluminescent Substrate for FCGBP and imaged on a Bio-Rad ChemiDoc system. ..

    Article Title: Periodontal pathogen Tannerella forsythia promotes liver fibrosis via ICAM-1⁺ neutrophil–dependent hepatic stellate cell activation
    Article Snippet: .. Following incubation with HRPconjugated secondary antibodies for 1 h at room temperature, protein bands were visualized using a SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher) and imaged with a ChemiDoc MP System (Bio-Rad, CA, USA). .. Total RNA was extracted from tissues or cells using TRIzol reagent (Invitrogen, USA) and reverse-transcribed into cDNA with a PrimeScript RT reagent kit (Takara, Japan).

    Cell-Signaling:

    Article Title: Astroviruses use mucus secretion as a novel form of viral egress.
    Article Snippet: Membranes were blocked with 2.5% BSA for 1 hour at room temperature and then incubated with HAstV1 spike rabbit anti-sera (1:500, kind gift from Dr. Rebecca DuBois, University of California, Santa Cruz) or blocked with 5% milk and then incubated with FCGBP rabbit polyclonal antibody (1:500, Cloud-Clone Corp) overnight at 4°C. .. Membranes were washed three times for 5 minutes each in TBST, then incubated with anti-rabbit IgG HRP (1:1,000, Cell Signaling) for 1 hour at room temperature followed by Pierce ECL Western Blotting Substrate for HAstV1 capsid or Pierce SuperSignal West Pico PLUS Chemiluminescent Substrate for FCGBP and imaged on a Bio-Rad ChemiDoc system. ..

    Western Blot:

    Article Title: Astroviruses use mucus secretion as a novel form of viral egress.
    Article Snippet: Membranes were blocked with 2.5% BSA for 1 hour at room temperature and then incubated with HAstV1 spike rabbit anti-sera (1:500, kind gift from Dr. Rebecca DuBois, University of California, Santa Cruz) or blocked with 5% milk and then incubated with FCGBP rabbit polyclonal antibody (1:500, Cloud-Clone Corp) overnight at 4°C. .. Membranes were washed three times for 5 minutes each in TBST, then incubated with anti-rabbit IgG HRP (1:1,000, Cell Signaling) for 1 hour at room temperature followed by Pierce ECL Western Blotting Substrate for HAstV1 capsid or Pierce SuperSignal West Pico PLUS Chemiluminescent Substrate for FCGBP and imaged on a Bio-Rad ChemiDoc system. ..

    Immunodetection:

    Article Title: A bifunctional actuator to correct loss-of-function deficits in cardiac voltage-gated sodium channels
    Article Snippet: After Ponceau staining, membranes were incubated with anti-Na V 1.5 antibody (Invitrogen, PA-110346) overnight at 4°C, followed by HRP-conjugated anti-rabbit secondary antibody. .. Immunodetection for all blots was performed using SuperSignal West Dura substrate (Thermo Fisher Scientific) and imaged with a Bio-Rad ChemiDoc system. .. All statistical analyses were performed using GraphPad Prism 10.1.2.



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