anti mouse tnfα (fluidigm)

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fluidigm anti mouse tnfα

Anti Mouse Tnfα, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mp6/Anti-Mouse+TNF%CE%B1+(MP6-XT22)-141Pr/pmc09791398-5-0-7
Average 93 stars, based on 5 article reviews

anti mouse tnfα - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses"

Article Title: Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses

Journal: STAR Protocols

doi: 10.1016/j.xpro.2022.101938

Figure Legend Snippet:

Techniques Used: Recombinant, Staining, Red Blood Cell Lysis, Software, Cytometry

other:

Article Title: Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses
Article Snippet: Anti-mouse TNFα, Clone MP6-XT22, 1:50 dilution , Fluidigm , Cat#3162002C, Label 162Dy.

Staining:

Article Title: Functional characterization of the dural sinuses as a neuroimmune interface.
Article Snippet: All individual samples were combined in the same tube and subsequently stained as multiplexed samples by incubating for 30 min at RT with the following antibodies: anti-CD45-Yb-89 (clone 30-F11, Fluidigm 3089005B), anti-CD11b-Nd-143 (cloneM1/70, Fluidigm 3143015B), anti-FITCNd-144 (clone FIT22, Fluidigm 3144006B), anti-CD4-Nd-145 (clone RM4-5, Fluidigm 3145002B), anti-F4/80-Nd-146 (clone BM8, Fluidigm 3146008B), anti-Ly6G-Nd-148 (clone 1A8, Biolegend, Maxpar Ready, conjugated in-house), anti-CD19-Sm-149 (clone 6D5, Fluidigm 3149002B), anti-CD24-Nd-150 (clone M1/69, Fluidigm 3150009B), anti-CD64-Eu-151 (clone X54-5/7.1, Fluidigm 3151012B), anti-CD3e-Sm-152 (clone 145-2C11, Fluidigm 3152004B), anti-Ter119-Sm-154 (clone Ter119, Fluidigm 3154005B), anti-PE-Gd-156 (clone PE001, Fluidigm 3156005B), anti-CD103-Dy-162 (clone FIB504, Fluidigm 3162026B), anti-CD62L-Dy-164 (cloneMEL-14, Fluidigm 3164003B), anti-CD8a-Er-168 (clone SK1, Fluidigm 3168002B), anti-TCRb-Tm-169 (clone H57597, Fluidigm 3169002B), anti-CD49b-Er-170 (clone HMa2, Fluidigm, 3170008B), anti-CD44-Yb-171 (clone IM7, Fluidigm 3171003B), anti-CD86Yb-172 (clone GL1, Fluidigm 3172016B), anti-I-A/I-E-Yb-174 (clone M5/114.15.2, Fluidigm 3174003B), anti-CD127-Lu-175 (clone A7R34, Fluidigm 3175006B), anti-B220-Yb-176 (clone RA3-682, Fluidigm 3176002B) and anti-CD11c-Bi-209 (clone N418, Fluidigm 3209005B). .. The fixed cells were washed and stained for intracellular antigens inMPNuclear Antigen Staining Buffer Set (Fluidigm) for additional 30 min at RT with: anti-TNF-Pr-141 (clone MP6-XT22, Fluidigm 3141013B), anti-Foxp3-Gd-158 (clone FJK-16 s, Fluidigm 3158003A) anti-RORgt-Tb-159 (clone B2D, Fluidigm 3159019B), anti-T-bet-Ho-165 (clone 4B10, Maxpar Ready, conjugated inhouse), anti-IL4-Er-166 (clone 11B11, Fluidigm 3166003C) and anti-GATA3-Er-167 (clone TWAJ, Fluidigm 3167007A). .. Samples were washed twice in Nuclear Antigen Staining Buffer and incubated in 125 nM Ir-191/193 DNA intercalator solution (Cell-ID Intercalator-Ir in Maxpar Fix/Perm buffer, Fluidigm) overnight at 4 C. Before acquisition on a HeliosMass Cytometer (available at the University of Virginia FlowCytometry Core Facility), samples were washed twice withMP Fix/Perm buffer, MPwater andMPCell acquisition solution.

Article Title: Meningeal lymphatics modulate microglial activation and immunotherapy in Alzheimer’s disease
Article Snippet: All individual samples were combined in the same tube and subsequently stained as multiplexed samples by incubating for 30 min at RT with the following antibodies: anti-CD45-Yb-89 (clone 30-F11, Fluidigm 3089005B), anti-CD11b-Nd-143 (clone M1/70, Fluidigm 3143015B), anti-FITC-Nd-144 (clone FIT22, Fluidigm 3144006B), anti-CD4-Nd-145 (clone RM4-5, Fluidigm 3145002B), anti-F4/80-Nd-146 (clone BM8, Fluidigm 3146008B), anti-Ly6G-Nd-148 (clone 1A8, Maxpar Ready, conjugated in-house), anti-CD19-Sm-149 (clone 6D5, Fluidigm 3149002B), anti-CD24-Nd-150 (clone M1/69, Fluidigm 3150009B), anti-CD64-Eu-151 (clone X54-5/7.1, Fluidigm 3151012B), anti-CD3ε-Sm-152 (clone 145-2C11, Fluidigm 3152004B), anti-Ter119-Sm-154 (clone Ter119, Fluidigm 3154005B), anti-PE-Gd-156 (clone PE001, Fluidigm 3156005B), anti-CD103-Dy-162 (clone FIB504, Fluidigm 3162026B), anti-CD14-Dy-163 (clone M14-23, Biolegend, Ultra-LEAF, conjugated in-house), anti-CD62L-Dy-164 (clone MEL-14, Fluidigm 3164003B), anti-CD8α-Er-168 (clone SK1, Fluidigm 3168002B), anti-TCRβ-Tm-169 (clone H57597, Fluidigm 3169002B), anti-NK1.1-Er-170 (clone PK136, Fluidigm, 3170002B), anti-CD44-Yb-171 (clone IM7, Fluidigm 3171003B), anti-CD86-Yb-172 (clone GL1, Fluidigm 3172016B), anti-I-A/I-E-Yb-174 (clone M5/114.15.2, Fluidigm 3174003B), anti-CD127-Lu-175 (clone A7R34, Fluidigm 3175006B), anti-B220-Yb-176 (clone RA3-682, Fluidigm 3176002B) and anti-CD11c-Bi-209 (clone N418, Fluidigm 3209005B). .. The fixed cells were washed and stained for intracellular antigens in MP Nuclear Antigen Staining Buffer Set (Fluidigm) for additional 30 min at RT with: anti-TNF-Pr-141 (clone MP6-XT22, Fluidigm 3141013B), anti-FOXP3-Gd-158 (clone FJK-16s, Fluidigm 3158003A) anti-RORγt-Tb-159 (clone B2D, Fluidigm 3159019B), anti-T-bet-Ho-165 (clone 4B10, Maxpar Ready, conjugated in-house) and anti-GATA3-Er-167 (clone TWAJ, Fluidigm 3167007A). .. Samples were washed twice in Nuclear Antigen Staining Buffer and incubated in 125 nM Ir-191/193 DNA intercalator solution (Cell-ID Intercalator-Ir in Maxpar Fix/Perm buffer, Fluidigm) overnight at 4°C.

Article Title: Meningeal lymphatics affect microglia responses and anti-Aβ immunotherapy.
Article Snippet: All individual samples were combined in the same tube and subsequently stained as multiplexed samples by incubating for 30 min at RT with the following antibodies: anti-CD45-Yb-89 (clone 30-F11, Fluidigm 3089005B), anti-CD11b-Nd-143 (clone M1/70, Fluidigm 3143015B), anti-FITC-Nd-144 (clone FIT22, Fluidigm 3144006B), anti-CD4-Nd-145 (clone RM4-5, Fluidigm 3145002B), anti-F4/80-Nd-146 (clone BM8, Fluidigm 3146008B), anti-Ly6G-Nd-148 (clone 1A8, Maxpar Ready, conjugated in-house), anti-CD19-Sm-149 (clone 6D5, Fluidigm 3149002B), anti-CD24-Nd-150 (clone M1/69, Fluidigm 3150009B), anti-CD64-Eu-151 (clone X54-5/7.1, Fluidigm 3151012B), anti-CD3ε-Sm-152 (clone 145-2C11, Fluidigm 3152004B), anti-Ter119-Sm-154 (clone Ter119, Fluidigm 3154005B), anti-PE-Gd-156 (clone PE001, Fluidigm 3156005B), anti-CD103-Dy-162 (clone FIB504, Fluidigm 3162026B), anti-CD14-Dy-163 (clone M14-23, Biolegend, Ultra-LEAF, conjugated in-house), anti-CD62L-Dy-164 (clone MEL-14, Fluidigm 3164003B), anti-CD8α-Er-168 (clone SK1, Fluidigm 3168002B), anti-TCRβ-Tm-169 (clone H57597, Fluidigm 3169002B), anti-NK1.1-Er-170 (clone PK136, Fluidigm, 3170002B), anti-CD44-Yb-171 (clone IM7, Fluidigm 3171003B), anti-CD86-Yb-172 (clone GL1, Fluidigm 3172016B), anti-I-A/I-E-Yb-174 (clone M5/114.15.2, Fluidigm 3174003B), anti-CD127-Lu-175 (clone A7R34, Fluidigm 3175006B), anti-B220-Yb-176 (clone RA3-682, Fluidigm 3176002B) and anti-CD11c-Bi-209 (clone N418, Fluidigm 3209005B). .. The fixed cells were washed and stained for intracellular antigens in MP Nuclear Antigen Staining Buffer Set (Fluidigm) for additional 30 min at RT with: anti-TNF-Pr-141 (clone MP6-XT22, Fluidigm 3141013B), anti-FOXP3-Gd-158 (clone FJK-16 s, Fluidigm 3158003A) anti-RORγt-Tb-159 (clone B2D, Fluidigm 3159019B), anti-T-bet-Ho-165 (clone 4B10, Maxpar Ready, conjugated in-house) and anti-GATA3-Er-167 (clone TWAJ, Fluidigm 3167007A). .. Samples were washed twice in Nuclear Antigen Staining Buffer and incubated in 125 nM Ir-191/193 DNA intercalator solution (Cell-ID Intercalator-Ir in Maxpar Fix/Perm buffer, Fluidigm) overnight at 4 °C.

Marker:

Article Title: UPP1 promotes lung adenocarcinoma progression through the induction of an immunosuppressive microenvironment
Article Snippet: .. Marker Clone Metal Cat No. CD11b [MAC1] M1/70 110Cd (Fluidigm, 92J001110), 1:100 TCRb H57-597 143Nd (Fluidigm, 3143010B), 1:100 CD25 3C7 150Nd (Fluidigm, 3150002B), 1:100 CD3e 145-2C11 152Sm (Fluidigm, 3152004B), 1:100 PD-L1 MIH5 153Eu (Fluidigm, 3153031B), 1:100 PD-1 RMP1-30 159Tb (Fluidigm, 3159006B), 1:100 CD163 EPR19518 161Dy (Abcam, ab213612), 1:100 TIM3 RMT3-23 162Dy (Fluidigm, 3162029B), 1:100 CD8a 53-6.7 168Er (Fluidigm, 3168003B), 1:200 NK1.1 PK136 170Er (Fluidigm, 3170002B), 1:100 CD19 6D5 171Yb (Fluidigm, 92J025171), 1:200 CD4 RM4-5 172Yb (Fluidigm, 3172003B), 1:200 LAG3 C9B7W 174Yb (Fluidigm, 3174019B), 1:100 CTLA-4 UC10-4B9 175Lu (Abcam, ab251599), 1:100 Ly-6G 1A8 195Pt (Fluidigm, 92J011195), 1:200 CD45 30-F11 89Y (Fluidigm, 3089005B), 1:200 TNFa MP6-XT22 141Pr (Fluidigm, 3141013B), 1:100 IL-4 11B11 155Gd (Biolegend, 504129), 1:100 IL-10 JES5-16E3 158Gd (Fluidigm, 3158002B), 1:100 IFNg XMG1.2 165Ho (Fluidigm, 3165003B), 1:100 FOXP3 FJK-16s 149Sm (Invitrogen, 14-5773-82), 1:100 Supplementary Table 2: PCR primers. ..

Polymerase Chain Reaction:

Mass Cytometry:

Article Title: Meningeal lymphatics affect microglia responses and anti-Aβ immunotherapy.
Article Snippet: - enrichment for brain cortical myeloid cells by cell sorting: anti-CD16/32 (101302, BioLegend); anti-CD11b-PerCP-Cy5.5 (550993, BD Biosciences), anti-CD45-A700 (560510, BD Biosciences) and anti-Ly6G-BV421 (562737, BD Biosciences), all at 1:200 in FACS buffer. .. The following antibodies were used for mass cytometry: - anti-Ly6C-Nd-142 (clone HK1.4, Maxpar Ready, Biolegend, conjugated in-house), anti-CD169-Sm-147 (clone H1.2F3, Maxpar Ready, conjugated in-house), anti-XCR1-Eu-153 (clone ZET, BioLegend, conjugated in-house), anti-Siglec-H-Gd-160 (clone 551, Biolegend, conjugated in-house), anti-FcER1-Dy-161 (clone MAR-1, Maxpar Ready, conjugated in-house), anti-PD-1-Er-166 (clone CHBC0116081, R&D systems, conjugated in-house), anti-H-2Kb/Db-Yb-173 (clone 28-8-6, BioLegend, conjugated in-house), antiCCR2-FITC (clone 475301, R&D Systems), anti-Thy1.2-PE (clone 30-H12, eBioscience), anti-CD45-Yb-89 (clone 30-F11, Fluidigm 3089005B), anti-CD11b-Nd-143 (clone M1/70, Fluidigm 3143015B), anti-FITC-Nd-144 (clone FIT22, Fluidigm 3144006B), antiCD4-Nd-145 (clone RM4-5, Fluidigm 3145002B), anti-F4/80-Nd-146 (clone BM8, Fluidigm 3146008B), anti-Ly6G-Nd-148 (clone 1A8, Maxpar Ready, conjugated in-house), anti-CD19-Sm-149 (clone 6D5, Fluidigm 3149002B), anti-CD24-Nd-150 (clone M1/69, Fluidigm 3150009B), anti-CD64-Eu-151 (clone X54-5/7.1, Fluidigm 3151012B), anti-CD3e-Sm-152 (clone 145-2C11, Fluidigm 3152004B), anti-Ter119-Sm-154 (clone Ter119, Fluidigm 3154005B), anti-PE-Gd-156 (clone PE001, Fluidigm 3156005B), antiCD103-Dy-162 (clone FIB504, Fluidigm 3162026B), anti-CD14-Dy-163 (clone M14-23, Biolegend, Ultra-LEAF, conjugated inhouse), anti-CD62L-Dy-164 (clone MEL-14, Fluidigm 3164003B), anti-CD8a-Er-168 (clone SK1, Fluidigm 3168002B), anti-TCRbTm-169 (clone H57597, Fluidigm 3169002B), anti-NK1.1-Er-170 (clone PK136, Fluidigm, 3170002B), anti-CD44-Yb-171 (clone IM7, Fluidigm 3171003B), anti-CD86-Yb-172 (clone GL1, Fluidigm 3172016B), anti-I-A/I-E-Yb-174 (clone M5/114.15.2, Fluidigm 3174003B), anti-CD127-Lu-175 (clone A7R34, Fluidigm 3175006B), anti-B220-Yb-176 (clone RA3-682, Fluidigm 3176002B), antiCD11c-Bi-209 (clone N418, Fluidigm 3209005B), anti-TNF-Pr-141 (clone MP6-XT22, Fluidigm 3141013B), anti-FOXP3-Gd-158 (clone FJK-16s, Fluidigm 3158003A) anti-RORgt-Tb-159 (clone B2D, Fluidigm 3159019B), anti-T-bet-Ho-165 (clone 4B10, Maxpar Ready, conjugated in-house) and anti-GATA3-Er-167 (clone TWAJ, Fluidigm 3167007A). ..

Review