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Novus Biologicals antibody against znf263

<t>ZNF263</t> binding is interrupted in asthmatic patients due to increased methylation at site cg11797365 leading to a reduced COL4A3 expression. a) Detection hits of chromatin-immunoprecipitation sequencing (ChIP-seq) data of ZNF263 in COL4A3 region. Large peak corresponds to region of first exon and start of first intron. cg11797365 is indicated by the red arrow and corresponds with previous report on ZNF263 binding (ENCODE track). The methylation site cg11797365 is close to the consensus binding site (green highlight in ENCODE track) of ZNF263. Custom track from ChIP-seq data from normal human bronchial epithelial cells (NHBE) is shown as indicated. b) Quantitative ChIP-seq analysis of ZNF263 binding to genomic region COL4A3 (1. intron) of cg11797365 in human bronchial epithelial cells (BEAS-2B) and NHBEs. Normalised binding events (per 1000 cells), n=4 per cell line (BEAS-2B and NHBE; Lonza). c) Quantitative (q)PCR to quantify ZNF263 expression levels in HeLA cells transfected with small interfering (si)RNA against ZNF263 in comparison to cells transfected with a nontarget control. d) Expression of COL4A3 in HeLA cells transfected with siRNA against ZNF263 compared to cells transfected with a nontarget control. Δct values are normalised to GAPDH . *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0.0001, Mann–Whitney U-test.

Antibody Against Znf263, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+znf263+coding+sequence/pmc08181658-106-22-25?v=Novus+Biologicals
Average 91 stars, based on 1 article reviews

antibody against znf263 - by Bioz Stars, 2026-08

91/100 stars

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1) Product Images from "COL4A3 expression in asthmatic epithelium depends on intronic methylation and ZNF263 binding"

Article Title: COL4A3 expression in asthmatic epithelium depends on intronic methylation and ZNF263 binding

Journal: ERJ Open Research

doi: 10.1183/23120541.00802-2020

ZNF263 binding is interrupted in asthmatic patients due to increased methylation at site cg11797365 leading to a reduced COL4A3 expression. a) Detection hits of chromatin-immunoprecipitation sequencing (ChIP-seq) data of ZNF263 in COL4A3 region. Large peak corresponds to region of first exon and start of first intron. cg11797365 is indicated by the red arrow and corresponds with previous report on ZNF263 binding (ENCODE track). The methylation site cg11797365 is close to the consensus binding site (green highlight in ENCODE track) of ZNF263. Custom track from ChIP-seq data from normal human bronchial epithelial cells (NHBE) is shown as indicated. b) Quantitative ChIP-seq analysis of ZNF263 binding to genomic region COL4A3 (1. intron) of cg11797365 in human bronchial epithelial cells (BEAS-2B) and NHBEs. Normalised binding events (per 1000 cells), n=4 per cell line (BEAS-2B and NHBE; Lonza). c) Quantitative (q)PCR to quantify ZNF263 expression levels in HeLA cells transfected with small interfering (si)RNA against ZNF263 in comparison to cells transfected with a nontarget control. d) Expression of COL4A3 in HeLA cells transfected with siRNA against ZNF263 compared to cells transfected with a nontarget control. Δct values are normalised to GAPDH . *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0.0001, Mann–Whitney U-test.

Figure Legend Snippet: ZNF263 binding is interrupted in asthmatic patients due to increased methylation at site cg11797365 leading to a reduced COL4A3 expression. a) Detection hits of chromatin-immunoprecipitation sequencing (ChIP-seq) data of ZNF263 in COL4A3 region. Large peak corresponds to region of first exon and start of first intron. cg11797365 is indicated by the red arrow and corresponds with previous report on ZNF263 binding (ENCODE track). The methylation site cg11797365 is close to the consensus binding site (green highlight in ENCODE track) of ZNF263. Custom track from ChIP-seq data from normal human bronchial epithelial cells (NHBE) is shown as indicated. b) Quantitative ChIP-seq analysis of ZNF263 binding to genomic region COL4A3 (1. intron) of cg11797365 in human bronchial epithelial cells (BEAS-2B) and NHBEs. Normalised binding events (per 1000 cells), n=4 per cell line (BEAS-2B and NHBE; Lonza). c) Quantitative (q)PCR to quantify ZNF263 expression levels in HeLA cells transfected with small interfering (si)RNA against ZNF263 in comparison to cells transfected with a nontarget control. d) Expression of COL4A3 in HeLA cells transfected with siRNA against ZNF263 compared to cells transfected with a nontarget control. Δct values are normalised to GAPDH . *: p<0.05, **: p<0.01, ***: p<0.001, ****: p<0.0001, Mann–Whitney U-test.

Techniques Used: Binding Assay, Methylation, Expressing, ChIP-sequencing, Transfection, Comparison, Control, MANN-WHITNEY

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(A) Venn diagram showing RAD21, CTCF, and PATZ1 binding in HEK293 cells. (B) Heat maps of RAD21, CTCF, PATZ1, MAZ, and other zinc finger proteins, ZNF263, ZNF341 and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, and Cluster 3 based on the indicated overlaps with RAD21 signal within a 4 kb window. (C) Western blot analysis of RAD21, FLAG, and CTCF upon FLAG-PATZ1 immunoprecipitation from mESCs (n=2, see for biological replicate). (D) Visualization of Hi-C contact matrices for a zoomed-in region around the TBC1D1 locus in HepG2 cells. Shown below are loops with PATZ1 at both anchors in HepG2 cells, ChIP-seq read densities for RAD21, CTCF, PATZ1, and MAZ, and gene annotations. ChIP-seq data in HepG2 cells is from two combined biological replicates. (E) Percentage of Hi-C loops in HepG2 cells overlapping with RAD21, CTCF, and PATZ1 ChIP-seq peaks. (F) Heat maps of RAD21, PATZ1, ZNF263, ZNF341, and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, Cluster 3, and Cluster 4 based on the combinatorial overlaps of zinc finger proteins with RAD21 within a 4 kb window in HEK293 cells. The model on the right side indicates combinatorial binding of the indicated factors in each cluster (see ). ChIP-seq data in HEK293 cells is from one replicate for RAD21 and one representative of two biological replicates for others (see for datasets).

Journal: Molecular cell

Article Title: Members of an array of zinc finger proteins specify distinct Hox chromatin boundaries

doi: 10.1016/j.molcel.2024.08.007

Figure Lengend Snippet: (A) Venn diagram showing RAD21, CTCF, and PATZ1 binding in HEK293 cells. (B) Heat maps of RAD21, CTCF, PATZ1, MAZ, and other zinc finger proteins, ZNF263, ZNF341 and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, and Cluster 3 based on the indicated overlaps with RAD21 signal within a 4 kb window. (C) Western blot analysis of RAD21, FLAG, and CTCF upon FLAG-PATZ1 immunoprecipitation from mESCs (n=2, see for biological replicate). (D) Visualization of Hi-C contact matrices for a zoomed-in region around the TBC1D1 locus in HepG2 cells. Shown below are loops with PATZ1 at both anchors in HepG2 cells, ChIP-seq read densities for RAD21, CTCF, PATZ1, and MAZ, and gene annotations. ChIP-seq data in HepG2 cells is from two combined biological replicates. (E) Percentage of Hi-C loops in HepG2 cells overlapping with RAD21, CTCF, and PATZ1 ChIP-seq peaks. (F) Heat maps of RAD21, PATZ1, ZNF263, ZNF341, and ZNF467 in HEK293 cells. ChIP-seq read density was grouped as Cluster 1, Cluster 2, Cluster 3, and Cluster 4 based on the combinatorial overlaps of zinc finger proteins with RAD21 within a 4 kb window in HEK293 cells. The model on the right side indicates combinatorial binding of the indicated factors in each cluster (see ). ChIP-seq data in HEK293 cells is from one replicate for RAD21 and one representative of two biological replicates for others (see for datasets).

Article Snippet: The mouse Znf263 coding sequence (NM_148924.3) with an HA-tag sequence fused to its 5’ end was cloned into a PiggyBac vector (pPB-CAG-FLAG-HA, a modified version of pPB-CAG-3xFLAG-empty-pgk-hph from Addgene, #48754) to create pPB-CAG-FLAG-HA-ZNF263 plasmid via Gibson assembly (NEB, #E2611).

Techniques: Binding Assay, ChIP-sequencing, Western Blot, Immunoprecipitation, Hi-C

(A) Normalized ChIP-seq densities for RAD21, CTCF, and PATZ1, and ZNF263 in WT, Patz1 KO, and Znf263 KO mESCs at the indicated regions in the HoxA cluster. ChIP-seq data represents one representative replicate of two biological replicates for RAD21, CTCF, and one replicate for FH-PATZ1 and FH-ZNF263. (B-D) RT-qPCR analysis for the indicated Hox genes in (B) the HoxA , (C) the HoxC , and (D) the HoxD clusters in WT and Patz1 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and ActB levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three biological replicates (two-sided Student’s t -test without multiple testing correction; *** P ≤ 0.001, ** P ≤ 0.01, * P < 0.05). (E) Differentially expressed genes by RNA-seq upon Patz1 KO in MNs from two biological replicates (see all in ). (F) GO analysis showing the top biological processes enriched in the differentially expressed genes in Patz1 KO versus WT MNs. PANTHER overrepresentation test tools were used for GO analysis and top 15 categories having a fold enrichment > 2.5 were plotted (see all in ). (G-I) RT-qPCR analysis for the indicated Hox genes in (G) the HoxA , (H) the HoxC , and (I) the HoxD clusters in WT and Znf263 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and Gapdh levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three technical replicates. Two independent Znf263 KO clones are shown (see ).

Journal: Molecular cell

Article Title: Members of an array of zinc finger proteins specify distinct Hox chromatin boundaries

doi: 10.1016/j.molcel.2024.08.007

Figure Lengend Snippet: (A) Normalized ChIP-seq densities for RAD21, CTCF, and PATZ1, and ZNF263 in WT, Patz1 KO, and Znf263 KO mESCs at the indicated regions in the HoxA cluster. ChIP-seq data represents one representative replicate of two biological replicates for RAD21, CTCF, and one replicate for FH-PATZ1 and FH-ZNF263. (B-D) RT-qPCR analysis for the indicated Hox genes in (B) the HoxA , (C) the HoxC , and (D) the HoxD clusters in WT and Patz1 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and ActB levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three biological replicates (two-sided Student’s t -test without multiple testing correction; *** P ≤ 0.001, ** P ≤ 0.01, * P < 0.05). (E) Differentially expressed genes by RNA-seq upon Patz1 KO in MNs from two biological replicates (see all in ). (F) GO analysis showing the top biological processes enriched in the differentially expressed genes in Patz1 KO versus WT MNs. PANTHER overrepresentation test tools were used for GO analysis and top 15 categories having a fold enrichment > 2.5 were plotted (see all in ). (G-I) RT-qPCR analysis for the indicated Hox genes in (G) the HoxA , (H) the HoxC , and (I) the HoxD clusters in WT and Znf263 KO cervical MNs. RT-qPCR signal was normalized to Atp5f1 and Gapdh levels. The fold-change in expression was calculated relative to WT MNs. All RT-qPCR results are represented as mean values and error bars indicating log 2 (SE) across three technical replicates. Two independent Znf263 KO clones are shown (see ).

Techniques: ChIP-sequencing, Quantitative RT-PCR, Expressing, RNA Sequencing, Clone Assay

Journal: Molecular cell

Article Title: Members of an array of zinc finger proteins specify distinct Hox chromatin boundaries

doi: 10.1016/j.molcel.2024.08.007

Figure Lengend Snippet: Key Resources Table

Techniques: Virus, Recombinant, Magnetic Beads, SYBR Green Assay, Western Blot, Cloning, Plasmid Preparation, Software, Injection

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