Review



c57bl6 mouse complement mc source  (Innovative Research Inc)


Bioz Verified Symbol Innovative Research Inc is a verified supplier
Bioz Manufacturer Symbol Innovative Research Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Innovative Research Inc c57bl6 mouse complement mc source
    C57bl6 Mouse Complement Mc Source, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+serum+complement/Innovative+Grade+US+Origin+Mouse+C57BL6+Complement+Serum/pm34696934-113-23-28
    Average 93 stars, based on 5 article reviews
    c57bl6 mouse complement mc source - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Bullous pemphigoid autoantibodies directly induce blister formation without complement activation.
    Article Snippet: .. The sections were washed three times with PBS and incubated with mouse serum complement (Innovative Research, Novi, MI) in a 1:50 dilution for 1 h at 37 ̊C. ..

    Article Title: An ELISA for detection of complement-bound circulating immune complexes in mice
    Article Snippet: .. In brief, mouse serum complement (Innovative Research, Novi, MI, cat. no IMS-C57BL6-COMPL) was incubated at 37 C for 90min with 1:100 HAGG (Complement Activator, Quidel, San Diego, CA, cat. #A114), followed by inactivation by addition of ice-cold 500mM ethylenediamine-tetraacetic acid (EDTA; to final concentration of 20mM) and storage (undiluted) at 80 C until analysis. ..

    Article Title: Intranasal vaccination with protein bodies elicit strong protection against Streptococcus pneumoniae colonization.
    Article Snippet: Bacteria were washed three times and resuspended in Hanks’ Balanced Salt Solution (HBSS, Gibco, Amarillo, Texas) without phenol red containing Ca2+/ Mg2+ + 0.1% gelatin (HBSS3+), at a concentration of 2 108 CFU/ mL. .. 10 mL bacteria was mixed with 10 mL 1:50 diluted serum collected 2 weeks after the third immunization and 1% final concentration of C57BL6 mouse Complement (mC) source (Innovative Research, Novi, Michigan) in HBSS3 + in a sterile 96 wells round bottom plate and incubated (30 min, 37 C, 220 rpm). ..

    Concentration Assay:

    Article Title: An ELISA for detection of complement-bound circulating immune complexes in mice
    Article Snippet: .. In brief, mouse serum complement (Innovative Research, Novi, MI, cat. no IMS-C57BL6-COMPL) was incubated at 37 C for 90min with 1:100 HAGG (Complement Activator, Quidel, San Diego, CA, cat. #A114), followed by inactivation by addition of ice-cold 500mM ethylenediamine-tetraacetic acid (EDTA; to final concentration of 20mM) and storage (undiluted) at 80 C until analysis. ..

    Article Title: Intranasal vaccination with protein bodies elicit strong protection against Streptococcus pneumoniae colonization.
    Article Snippet: Bacteria were washed three times and resuspended in Hanks’ Balanced Salt Solution (HBSS, Gibco, Amarillo, Texas) without phenol red containing Ca2+/ Mg2+ + 0.1% gelatin (HBSS3+), at a concentration of 2 108 CFU/ mL. .. 10 mL bacteria was mixed with 10 mL 1:50 diluted serum collected 2 weeks after the third immunization and 1% final concentration of C57BL6 mouse Complement (mC) source (Innovative Research, Novi, Michigan) in HBSS3 + in a sterile 96 wells round bottom plate and incubated (30 min, 37 C, 220 rpm). ..

    Cell Culture:

    Article Title: Pivotal advance: characterization of mouse liver phagocytic B cells in innate immunity.
    Article Snippet: Although B cells in vertebrates have been thought to lack phagocytic activity, there has been a recent report of such ability by the B cells of early vertebrates such as fish and frogs.. Here, we show for the first time that mouse liver IgM B cells actively phagocytose microsphere beads and Escherichia coli and that they effectively kill bacterial cells.. Such phagocytic activity is not observed in other liver MNCs, except for F4/80 Kupffer cells.

    Activity Assay:

    Article Title: Pivotal advance: characterization of mouse liver phagocytic B cells in innate immunity.
    Article Snippet: Although B cells in vertebrates have been thought to lack phagocytic activity, there has been a recent report of such ability by the B cells of early vertebrates such as fish and frogs.. Here, we show for the first time that mouse liver IgM B cells actively phagocytose microsphere beads and Escherichia coli and that they effectively kill bacterial cells.. Such phagocytic activity is not observed in other liver MNCs, except for F4/80 Kupffer cells.

    Bacteria:

    Article Title: Intranasal vaccination with protein bodies elicit strong protection against Streptococcus pneumoniae colonization.
    Article Snippet: Bacteria were washed three times and resuspended in Hanks’ Balanced Salt Solution (HBSS, Gibco, Amarillo, Texas) without phenol red containing Ca2+/ Mg2+ + 0.1% gelatin (HBSS3+), at a concentration of 2 108 CFU/ mL. .. 10 mL bacteria was mixed with 10 mL 1:50 diluted serum collected 2 weeks after the third immunization and 1% final concentration of C57BL6 mouse Complement (mC) source (Innovative Research, Novi, Michigan) in HBSS3 + in a sterile 96 wells round bottom plate and incubated (30 min, 37 C, 220 rpm). ..

    Sterility:

    Article Title: Intranasal vaccination with protein bodies elicit strong protection against Streptococcus pneumoniae colonization.
    Article Snippet: Bacteria were washed three times and resuspended in Hanks’ Balanced Salt Solution (HBSS, Gibco, Amarillo, Texas) without phenol red containing Ca2+/ Mg2+ + 0.1% gelatin (HBSS3+), at a concentration of 2 108 CFU/ mL. .. 10 mL bacteria was mixed with 10 mL 1:50 diluted serum collected 2 weeks after the third immunization and 1% final concentration of C57BL6 mouse Complement (mC) source (Innovative Research, Novi, Michigan) in HBSS3 + in a sterile 96 wells round bottom plate and incubated (30 min, 37 C, 220 rpm). ..

    Purification:

    Article Title: Identification of Two Variants of Acinetobacter baumannii Strain ATCC 17978 with Distinct Genotypes and Phenotypes
    Article Snippet: .. Purified neutrophils were seeded at a density of 1.6 105 to 3.2 105 cells/well in wells of a 96-well plate pretreated with 100 ml of 20% normal human serum, after which they were allowed to attach to well bottoms by incubating plates at room temperature for 30 min. A. baumanii 17978 VU, 17978 UN, and 17978 UN DkatX strains were opsonized by resuspension in 50% C57BL/6 mouse complement serum (Innovative Research, Novi, MI) in RPMI medium supplemented with 10 mM HEPES buffer (RPMI/H). ..



    Similar Products

    93
    Innovative Research Inc c57bl 6 mouse serum
    C57bl 6 Mouse Serum, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+serum+complement/Innovative+Grade+US+Origin+Mouse+C57BL6+Complement+Serum/pm40741974-252-16-23
    Average 93 stars, based on 1 article reviews
    c57bl 6 mouse serum - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    95
    Cedarlane heat inactivated terminal mouse serum
    A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
    Heat Inactivated Terminal Mouse Serum, supplied by Cedarlane, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+serum+complement/Baby+Rabbit+Complement%2C+Sterile%2C+Frozen/bio_rxiv__2025__10__30__685603-203-26-19
    Average 95 stars, based on 1 article reviews
    heat inactivated terminal mouse serum - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    90
    Thermo Fisher antiserum/complement mixture (1:20 dilution of the serum and 20% of mouse complement
    A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
    Antiserum/Complement Mixture (1:20 Dilution Of The Serum And 20% Of Mouse Complement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+serum+complement/pm39370617-339-12-26
    Average 90 stars, based on 1 article reviews
    antiserum/complement mixture (1:20 dilution of the serum and 20% of mouse complement - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    93
    Innovative Research Inc c57bl6 mouse complement mc source
    A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
    C57bl6 Mouse Complement Mc Source, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+serum+complement/Innovative+Grade+US+Origin+Mouse+C57BL6+Complement+Serum/pm34696934-113-23-28
    Average 93 stars, based on 1 article reviews
    c57bl6 mouse complement mc source - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    90
    Innovative Research Inc c57bl/6 mouse complement serum
    A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
    C57bl/6 Mouse Complement Serum, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+serum+complement/10__1128_slash_iai__00454___21-330-74-69
    Average 90 stars, based on 1 article reviews
    c57bl/6 mouse complement serum - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    93
    Innovative Research Inc c57bl 6 mouse complement serum
    A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
    C57bl 6 Mouse Complement Serum, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+serum+complement/Innovative+Grade+US+Origin+Mouse+C57BL6+Complement+Serum/10__1128_slash_iai__00454___21-330-65-69
    Average 93 stars, based on 1 article reviews
    c57bl 6 mouse complement serum - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    96
    Valiant Co Ltd mouse complement c3
    A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
    Mouse Complement C3, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+serum+complement/Mouse+serum/pm34211460-52-36-39
    Average 96 stars, based on 1 article reviews
    mouse complement c3 - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    91
    Valiant Co Ltd anti serum against mouse c3
    A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
    Anti Serum Against Mouse C3, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+serum+complement/IgG+Goat+Anti-Mouse+Complement+C3/pm32849588-90-19-23
    Average 91 stars, based on 1 article reviews
    anti serum against mouse c3 - by Bioz Stars, 2026-10
    91/100 stars
      Buy from Supplier

    Image Search Results


    A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat inactivated Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.

    Journal: bioRxiv

    Article Title: Rationally designed minimized TbpB confers broad protection against meningococcal infection

    doi: 10.1101/2025.10.30.685603

    Figure Lengend Snippet: A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat inactivated Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.

    Article Snippet: Assay was set up in 40 μL total volume using ∼500 bacteria suspended in PBS++, 10% baby rabbit complement (Cedarlane, CL3441-S100) and 2-fold serial dilutions of heat inactivated terminal mouse serum.

    Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay, Transformation Assay, Growth Inhibition Assay, Standard Deviation

    A: Phylogenetic tree depicting Neisserial TbpB diversity. The location of M982 TbpB is highlighted, along with 16 additional TbpBs spanning various clusters used for cross-reactivity analysis. B: Heat map depicting IgG cross-reactivity against a broad panel of TbpB protein variants by ELISA. Optical density (OD) readings denoted by the colour gradient. Background noise from no-protein control subtracted. Each row is a different TbpB protein capture, with the phylogenetic cluster indicated to the left. Each column is a different mouse sample, with the immunizing antigens indicated above. Numbered labels starting with A are pre-challenge serum from the immunized C67BL/6 sepsis cohort; B are terminal serum from the immunized hCEACAM1 FvB colonization cohort. C: Grouped bar graph summarizing cross-reactive antibody data from B. Bars represent mean, error bars represent standard error, each circle represents serum from a single mouse (N=8 for immune sera, N=3 for Alum). hTf-HRP signal quantifying properly folded TbpB capture is depicted in the inset, mean ± standard deviation. D: Serum reactivity against representative Nme and Ngo strains grown under iron limitation using inactivated whole bacterial ELISA. TbpB cluster indicated below the x-axis. Background noise from no serum control subtracted. Bars represent mean, error bars represent standard error, each circle represents serum from a single mouse, N=5-8/group. Inset depicts hTf-binding as an indicator of TbpAB receptor expression on bacteria used for coating ELISA plates, mean ± standard deviation. For C and D, two-way ANOVA with Dunnett’s post-hoc test comparing each group to Alum control performed using GraphPad Prism 10.2.0. Only p-values <0.05 shown. *, p<0.05; **, p<0.01; ***, p<0.001, ****, p<0.0001.

    Journal: bioRxiv

    Article Title: Rationally designed minimized TbpB confers broad protection against meningococcal infection

    doi: 10.1101/2025.10.30.685603

    Figure Lengend Snippet: A: Phylogenetic tree depicting Neisserial TbpB diversity. The location of M982 TbpB is highlighted, along with 16 additional TbpBs spanning various clusters used for cross-reactivity analysis. B: Heat map depicting IgG cross-reactivity against a broad panel of TbpB protein variants by ELISA. Optical density (OD) readings denoted by the colour gradient. Background noise from no-protein control subtracted. Each row is a different TbpB protein capture, with the phylogenetic cluster indicated to the left. Each column is a different mouse sample, with the immunizing antigens indicated above. Numbered labels starting with A are pre-challenge serum from the immunized C67BL/6 sepsis cohort; B are terminal serum from the immunized hCEACAM1 FvB colonization cohort. C: Grouped bar graph summarizing cross-reactive antibody data from B. Bars represent mean, error bars represent standard error, each circle represents serum from a single mouse (N=8 for immune sera, N=3 for Alum). hTf-HRP signal quantifying properly folded TbpB capture is depicted in the inset, mean ± standard deviation. D: Serum reactivity against representative Nme and Ngo strains grown under iron limitation using inactivated whole bacterial ELISA. TbpB cluster indicated below the x-axis. Background noise from no serum control subtracted. Bars represent mean, error bars represent standard error, each circle represents serum from a single mouse, N=5-8/group. Inset depicts hTf-binding as an indicator of TbpAB receptor expression on bacteria used for coating ELISA plates, mean ± standard deviation. For C and D, two-way ANOVA with Dunnett’s post-hoc test comparing each group to Alum control performed using GraphPad Prism 10.2.0. Only p-values <0.05 shown. *, p<0.05; **, p<0.01; ***, p<0.001, ****, p<0.0001.

    Article Snippet: Assay was set up in 40 μL total volume using ∼500 bacteria suspended in PBS++, 10% baby rabbit complement (Cedarlane, CL3441-S100) and 2-fold serial dilutions of heat inactivated terminal mouse serum.

    Techniques: Enzyme-linked Immunosorbent Assay, Control, Standard Deviation, Binding Assay, Expressing, Bacteria