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Valiant Co Ltd mouse complement c3
Mouse Complement C3, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+serum+complement/Mouse+serum/pm34211460-52-36-39
Average 96 stars, based on 93 article reviews
mouse complement c3 - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Activity Assay:

Article Title: Intranasal vaccines adjuvanted with Nexavant demonstrate robust protective efficacy by inducing both mucosal and systemic immunity in a murine model
Article Snippet: .. Serum bactericidal activity against Neisseria meningitidis serogroup C was assessed using heat-inactivated mouse serum and baby rabbit complement (BRC; MP Biomedicals), following a standardized protocol. ..

Western Blot:

Article Title: Manipulating the EphB4-ephrinB2 axis to reduce metastasis in HNSCC
Article Snippet: To assign the right protein size, Precision Plus Protein Dual Color Standards Protein Marker was used as a marker (6 μl, #1610374, BIO-RAD). .. For immunoblotting of Fc fusion proteins in vivo, mouse serum and purified human IgG Fc (#0855911, MP Biomedicals, Irvine, California, USA) were diluted in LDS sample buffer (#B0007, Life Technologies, Carlsbad, California, USA) with 2.5% β-mercaptoethanol, heated at 95 °C for 2 min, and run on Bolt Bis-Tris Plus gels (#NW04125Box, ThermoFisher Scientific). .. After semi-dry transfer, the Immobilon membranes were blocked with 5% BSA in 0.1% Tween 20 in Tris buffered saline (TBS) for 1 h and then incubated in the cold overnight with an anti-human IgG antibody at a 1:1000 dilution (#109-005-098, Jackson ImmunoResearch Labs, West Grove, Pennsylvania, USA).

Article Title: Manipulating the EphB4-ephrinB2 axis to reduce metastasis in HNSCC.
Article Snippet: To assign the right protein size, Precision Plus Protein Dual Color Standards Protein Marker was used as a marker (6 μl, #1610374, BIO-RAD). .. For immunoblotting of Fc fusion proteins in vivo, mouse serum and purified human IgG Fc (#0855911, MP Biomedicals, Irvine, California, USA) were diluted in LDS sample buffer (#B0007, Life Technologies, Carlsbad, California, USA) with 2.5% β-mercaptoethanol, heated at 95 °C for 2 min, and run on Bolt Bis-Tris Plus gels (#NW04125Box, ThermoFisher Scientific). .. After semi-dry transfer, the Immobilon membranes were blocked with 5% BSA in 0.1% Tween 20 in Tris buffered saline (TBS) for 1 h and then incubated in the cold overnight with an anti-human IgG antibody at a 1:1000 dilution (#109-005-098, Jackson ImmunoResearch Labs, West Grove, Pennsylvania, USA).

In Vivo:

Article Title: Manipulating the EphB4-ephrinB2 axis to reduce metastasis in HNSCC
Article Snippet: To assign the right protein size, Precision Plus Protein Dual Color Standards Protein Marker was used as a marker (6 μl, #1610374, BIO-RAD). .. For immunoblotting of Fc fusion proteins in vivo, mouse serum and purified human IgG Fc (#0855911, MP Biomedicals, Irvine, California, USA) were diluted in LDS sample buffer (#B0007, Life Technologies, Carlsbad, California, USA) with 2.5% β-mercaptoethanol, heated at 95 °C for 2 min, and run on Bolt Bis-Tris Plus gels (#NW04125Box, ThermoFisher Scientific). .. After semi-dry transfer, the Immobilon membranes were blocked with 5% BSA in 0.1% Tween 20 in Tris buffered saline (TBS) for 1 h and then incubated in the cold overnight with an anti-human IgG antibody at a 1:1000 dilution (#109-005-098, Jackson ImmunoResearch Labs, West Grove, Pennsylvania, USA).

Article Title: Manipulating the EphB4-ephrinB2 axis to reduce metastasis in HNSCC.
Article Snippet: To assign the right protein size, Precision Plus Protein Dual Color Standards Protein Marker was used as a marker (6 μl, #1610374, BIO-RAD). .. For immunoblotting of Fc fusion proteins in vivo, mouse serum and purified human IgG Fc (#0855911, MP Biomedicals, Irvine, California, USA) were diluted in LDS sample buffer (#B0007, Life Technologies, Carlsbad, California, USA) with 2.5% β-mercaptoethanol, heated at 95 °C for 2 min, and run on Bolt Bis-Tris Plus gels (#NW04125Box, ThermoFisher Scientific). .. After semi-dry transfer, the Immobilon membranes were blocked with 5% BSA in 0.1% Tween 20 in Tris buffered saline (TBS) for 1 h and then incubated in the cold overnight with an anti-human IgG antibody at a 1:1000 dilution (#109-005-098, Jackson ImmunoResearch Labs, West Grove, Pennsylvania, USA).

Purification:

Article Title: Manipulating the EphB4-ephrinB2 axis to reduce metastasis in HNSCC
Article Snippet: To assign the right protein size, Precision Plus Protein Dual Color Standards Protein Marker was used as a marker (6 μl, #1610374, BIO-RAD). .. For immunoblotting of Fc fusion proteins in vivo, mouse serum and purified human IgG Fc (#0855911, MP Biomedicals, Irvine, California, USA) were diluted in LDS sample buffer (#B0007, Life Technologies, Carlsbad, California, USA) with 2.5% β-mercaptoethanol, heated at 95 °C for 2 min, and run on Bolt Bis-Tris Plus gels (#NW04125Box, ThermoFisher Scientific). .. After semi-dry transfer, the Immobilon membranes were blocked with 5% BSA in 0.1% Tween 20 in Tris buffered saline (TBS) for 1 h and then incubated in the cold overnight with an anti-human IgG antibody at a 1:1000 dilution (#109-005-098, Jackson ImmunoResearch Labs, West Grove, Pennsylvania, USA).

Article Title: Manipulating the EphB4-ephrinB2 axis to reduce metastasis in HNSCC.
Article Snippet: To assign the right protein size, Precision Plus Protein Dual Color Standards Protein Marker was used as a marker (6 μl, #1610374, BIO-RAD). .. For immunoblotting of Fc fusion proteins in vivo, mouse serum and purified human IgG Fc (#0855911, MP Biomedicals, Irvine, California, USA) were diluted in LDS sample buffer (#B0007, Life Technologies, Carlsbad, California, USA) with 2.5% β-mercaptoethanol, heated at 95 °C for 2 min, and run on Bolt Bis-Tris Plus gels (#NW04125Box, ThermoFisher Scientific). .. After semi-dry transfer, the Immobilon membranes were blocked with 5% BSA in 0.1% Tween 20 in Tris buffered saline (TBS) for 1 h and then incubated in the cold overnight with an anti-human IgG antibody at a 1:1000 dilution (#109-005-098, Jackson ImmunoResearch Labs, West Grove, Pennsylvania, USA).

Staining:

Article Title: Proteins having pneumococcal capsule degrading activity and methods of use
Article Snippet: .. Aliquots (in duplicate wells on a 96-well round-bottom plate) were stained with Hoechst 33342 and treated with inactivated or functional Pn3Pase at 5 or 50 μg/ml for 1 hour at 37° C. Normal mouse serum (1:10 dilution) was added to the samples for 30 minutes at 37° C. Cells were washed and stained with FITC-conjugated goat antibody to mouse complement (MP BioMedical, Santa Ana, CA) at 4° C. for 30 minutes. ..

Functional Assay:

Article Title: Proteins having pneumococcal capsule degrading activity and methods of use
Article Snippet: .. Aliquots (in duplicate wells on a 96-well round-bottom plate) were stained with Hoechst 33342 and treated with inactivated or functional Pn3Pase at 5 or 50 μg/ml for 1 hour at 37° C. Normal mouse serum (1:10 dilution) was added to the samples for 30 minutes at 37° C. Cells were washed and stained with FITC-conjugated goat antibody to mouse complement (MP BioMedical, Santa Ana, CA) at 4° C. for 30 minutes. ..

Sterility:

Article Title: Engineered bacteria for near-infrared light-inducible expression of cancer therapeutics.
Article Snippet: Bacteria-based therapies hold great promise for cancer treatment due to their selective tumor colonization and proliferation.. However, clinical application is hindered by the need for safe, precise control systems to regulate local therapeutic payload expression and release.. Here we developed a near-infrared (NIR) light-mediated PadC-based photoswitch (NETMAP) system based on a chimeric phytochrome-activated diguanylyl cyclase (PadC) and a cyclic diguanylate monophosphate-dependent transcriptional activator (MrkH).



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A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
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A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
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A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
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A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
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A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
Mouse Complement C3, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat <t>inactivated</t> Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.
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Image Search Results


A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat inactivated Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.

Journal: bioRxiv

Article Title: Rationally designed minimized TbpB confers broad protection against meningococcal infection

doi: 10.1101/2025.10.30.685603

Figure Lengend Snippet: A-C: Protein ELISA measuring reactivity of TbpB, C-lobe, and LCL-immune sera against full-length TbpB, TbpB C-lobe, and TbpB N-lobe, respectively. D: IgG titre elicited by the different immunogens towards heat inactivated Nme M982. A-D: N=10-15 per group; squares represent pre-challenge sera from C57BL/6 animals from the sepsis study presented in ; circles represent terminal serum from hCEACAM1 FvB mice from the colonization study presented in , each symbol represents serum from one animal tested in duplicate. E: Serum bactericidal activity (SBA) titre against iron-starved Nme M982. N=19-25 per group, including terminal hCEACAM1 FvB serum from , plus additional terminal serum from immunized hCEACAM1 FvB from pilot studies. SBA titre (>50% killing) reached for 19/25 (76%) TbpB, 8/19 (42%) C-lobe, 14/22 (64%) LCL and 3/21 (14%) Alum samples. Dotted lines represent lowest dilution tested. Line at median for each group. One-way ANOVA with Tukey’s post-hoc test comparing each group to every other group performed using GraphPad Prism 10.2.0. For D, E: Statistics performed on Log2 transformed data. ns, not significant; **, p<0.01; ***, p<0.001; ****, p<0.0001. F: Meningococcal growth inhibition assay. Iron-starved M982 strain was grown in the presence of hTf and pooled heat-inactivated serum from TbpB, C-lobe and LCL immunized animals from . Change in absorbance (OD600) relative to the initial time point is graphed; error bars depict standard deviation of two technical replicates.

Article Snippet: Assay was set up in 40 μL total volume using ∼500 bacteria suspended in PBS++, 10% baby rabbit complement (Cedarlane, CL3441-S100) and 2-fold serial dilutions of heat inactivated terminal mouse serum.

Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay, Transformation Assay, Growth Inhibition Assay, Standard Deviation

A: Phylogenetic tree depicting Neisserial TbpB diversity. The location of M982 TbpB is highlighted, along with 16 additional TbpBs spanning various clusters used for cross-reactivity analysis. B: Heat map depicting IgG cross-reactivity against a broad panel of TbpB protein variants by ELISA. Optical density (OD) readings denoted by the colour gradient. Background noise from no-protein control subtracted. Each row is a different TbpB protein capture, with the phylogenetic cluster indicated to the left. Each column is a different mouse sample, with the immunizing antigens indicated above. Numbered labels starting with A are pre-challenge serum from the immunized C67BL/6 sepsis cohort; B are terminal serum from the immunized hCEACAM1 FvB colonization cohort. C: Grouped bar graph summarizing cross-reactive antibody data from B. Bars represent mean, error bars represent standard error, each circle represents serum from a single mouse (N=8 for immune sera, N=3 for Alum). hTf-HRP signal quantifying properly folded TbpB capture is depicted in the inset, mean ± standard deviation. D: Serum reactivity against representative Nme and Ngo strains grown under iron limitation using inactivated whole bacterial ELISA. TbpB cluster indicated below the x-axis. Background noise from no serum control subtracted. Bars represent mean, error bars represent standard error, each circle represents serum from a single mouse, N=5-8/group. Inset depicts hTf-binding as an indicator of TbpAB receptor expression on bacteria used for coating ELISA plates, mean ± standard deviation. For C and D, two-way ANOVA with Dunnett’s post-hoc test comparing each group to Alum control performed using GraphPad Prism 10.2.0. Only p-values <0.05 shown. *, p<0.05; **, p<0.01; ***, p<0.001, ****, p<0.0001.

Journal: bioRxiv

Article Title: Rationally designed minimized TbpB confers broad protection against meningococcal infection

doi: 10.1101/2025.10.30.685603

Figure Lengend Snippet: A: Phylogenetic tree depicting Neisserial TbpB diversity. The location of M982 TbpB is highlighted, along with 16 additional TbpBs spanning various clusters used for cross-reactivity analysis. B: Heat map depicting IgG cross-reactivity against a broad panel of TbpB protein variants by ELISA. Optical density (OD) readings denoted by the colour gradient. Background noise from no-protein control subtracted. Each row is a different TbpB protein capture, with the phylogenetic cluster indicated to the left. Each column is a different mouse sample, with the immunizing antigens indicated above. Numbered labels starting with A are pre-challenge serum from the immunized C67BL/6 sepsis cohort; B are terminal serum from the immunized hCEACAM1 FvB colonization cohort. C: Grouped bar graph summarizing cross-reactive antibody data from B. Bars represent mean, error bars represent standard error, each circle represents serum from a single mouse (N=8 for immune sera, N=3 for Alum). hTf-HRP signal quantifying properly folded TbpB capture is depicted in the inset, mean ± standard deviation. D: Serum reactivity against representative Nme and Ngo strains grown under iron limitation using inactivated whole bacterial ELISA. TbpB cluster indicated below the x-axis. Background noise from no serum control subtracted. Bars represent mean, error bars represent standard error, each circle represents serum from a single mouse, N=5-8/group. Inset depicts hTf-binding as an indicator of TbpAB receptor expression on bacteria used for coating ELISA plates, mean ± standard deviation. For C and D, two-way ANOVA with Dunnett’s post-hoc test comparing each group to Alum control performed using GraphPad Prism 10.2.0. Only p-values <0.05 shown. *, p<0.05; **, p<0.01; ***, p<0.001, ****, p<0.0001.

Article Snippet: Assay was set up in 40 μL total volume using ∼500 bacteria suspended in PBS++, 10% baby rabbit complement (Cedarlane, CL3441-S100) and 2-fold serial dilutions of heat inactivated terminal mouse serum.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Standard Deviation, Binding Assay, Expressing, Bacteria