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10X Genomics mouse reference transcriptome
Mouse Reference Transcriptome, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+reference+transcriptome/mouse+transcriptome/pmc11866521-462-10-17
Average 86 stars, based on 1 article reviews
mouse reference transcriptome - by Bioz Stars, 2026-10
86/100 stars

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Article Title: Perturb-Multimodal: a platform for pooled genetic screens with sequencing and imaging in intact mammalian tissue
Article Snippet: Chromium Fixed RNA Kit, Mouse Transcriptome, 4rxns x 4 BC , 10x Genomics , Cat#: 1000496.

Article Title: An ex vivo uterine system captures implantation, embryogenesis, and trophoblast invasion via maternal-embryonic signaling
Article Snippet: Then, paired-end sequencing was performed on a next-generation sequencer NovaSeq X Plus (Illumina, USA).

Sequencing:

Article Title: Iba1 deficiency impairs microglial synaptic remodeling and neuronal survival after axonal injury.
Article Snippet: .. Subsequent steps—including sample pooling, generation of single-nucleus emulsions, and sequencing library preparation—were performed using the Chromium Fixed RNA Kit for Mouse Transcriptome (10x Genomics, #1000496). .. The resulting sequencing libraries were quality-controlled using a Qubit dsDNA Assay Kit (Thermo Fisher Scientific) and a TapeStation D1000 System (Agilent Technologies), and sequencing was performed on an Illumina NovaSeq 6000 platform.

Cell Counting:

Article Title: An ex vivo uterine system captures implantation, embryogenesis, and trophoblast invasion via maternal-embryonic signaling.
Article Snippet: For embryonic scRNA-seq, E4.5 in vivo embryos and 24h ex vivo embryos were microscopically isolated, immediately soaked in Accumax (17087-54, Nacalai Tesque, Japan) at room temperature, and dissociated with glass capillaries. .. After cell counting, the single-cell suspension was treated for RNA sequencing library preparation with Chromium Fixed RNA Kit, Mouse Transcriptome (PN1000496, 10x genomics, USA), Chromium Mouse Transcriptome Probe Set v1.0.1 (10x genomics, USA), andChromiumX (10x genomics, USA). .. The singlecell suspension from a total of 33 in vivo embryos at E4.5 from 4 mice and 32 ex vivo embryos at 24 h from 4 donor mice was immediately processed for RNA sequencing library preparation using Chromium Next GEM Single Cell 3’ Library and Gel BeadKit v3.1 (PN-1000268, 10x genomics, USA), Chromium Next GEM Chip G Single Cell Kit (PN1000120, 10x genomics, USA), and Chromium iX (10x genomics, USA).

Single Cell:

Article Title: An ex vivo uterine system captures implantation, embryogenesis, and trophoblast invasion via maternal-embryonic signaling.
Article Snippet: For embryonic scRNA-seq, E4.5 in vivo embryos and 24h ex vivo embryos were microscopically isolated, immediately soaked in Accumax (17087-54, Nacalai Tesque, Japan) at room temperature, and dissociated with glass capillaries. .. After cell counting, the single-cell suspension was treated for RNA sequencing library preparation with Chromium Fixed RNA Kit, Mouse Transcriptome (PN1000496, 10x genomics, USA), Chromium Mouse Transcriptome Probe Set v1.0.1 (10x genomics, USA), andChromiumX (10x genomics, USA). .. The singlecell suspension from a total of 33 in vivo embryos at E4.5 from 4 mice and 32 ex vivo embryos at 24 h from 4 donor mice was immediately processed for RNA sequencing library preparation using Chromium Next GEM Single Cell 3’ Library and Gel BeadKit v3.1 (PN-1000268, 10x genomics, USA), Chromium Next GEM Chip G Single Cell Kit (PN1000120, 10x genomics, USA), and Chromium iX (10x genomics, USA).

Article Title: Mapping neuro-vascular unit communications reveals distinct angiogenic programs across developing mouse brain regions.
Article Snippet: .. Multiplexed libraries were prepared using the Chromium Next GEM Single Cell Fixed RNA Kit, Mouse Transcriptome, 4rxns x 4 BC (# 1000496, 10x Genomics; Pleasanton, CA, USA) AR TI CL E IN P RE SS according to the manufacturer’s instructions. .. After a PBS step washing, the myelin and the cell debris were separated from the pelleted cells by using the debris removal solution (Miltenyi Biotec, 130-109-398) and the cells were washed with PBS before proceeding to the red blood cell lysis (Miltenyi Biotec, 130-094-183).

Suspension:

Article Title: An ex vivo uterine system captures implantation, embryogenesis, and trophoblast invasion via maternal-embryonic signaling.
Article Snippet: For embryonic scRNA-seq, E4.5 in vivo embryos and 24h ex vivo embryos were microscopically isolated, immediately soaked in Accumax (17087-54, Nacalai Tesque, Japan) at room temperature, and dissociated with glass capillaries. .. After cell counting, the single-cell suspension was treated for RNA sequencing library preparation with Chromium Fixed RNA Kit, Mouse Transcriptome (PN1000496, 10x genomics, USA), Chromium Mouse Transcriptome Probe Set v1.0.1 (10x genomics, USA), andChromiumX (10x genomics, USA). .. The singlecell suspension from a total of 33 in vivo embryos at E4.5 from 4 mice and 32 ex vivo embryos at 24 h from 4 donor mice was immediately processed for RNA sequencing library preparation using Chromium Next GEM Single Cell 3’ Library and Gel BeadKit v3.1 (PN-1000268, 10x genomics, USA), Chromium Next GEM Chip G Single Cell Kit (PN1000120, 10x genomics, USA), and Chromium iX (10x genomics, USA).

RNA Sequencing:

Article Title: An ex vivo uterine system captures implantation, embryogenesis, and trophoblast invasion via maternal-embryonic signaling.
Article Snippet: For embryonic scRNA-seq, E4.5 in vivo embryos and 24h ex vivo embryos were microscopically isolated, immediately soaked in Accumax (17087-54, Nacalai Tesque, Japan) at room temperature, and dissociated with glass capillaries. .. After cell counting, the single-cell suspension was treated for RNA sequencing library preparation with Chromium Fixed RNA Kit, Mouse Transcriptome (PN1000496, 10x genomics, USA), Chromium Mouse Transcriptome Probe Set v1.0.1 (10x genomics, USA), andChromiumX (10x genomics, USA). .. The singlecell suspension from a total of 33 in vivo embryos at E4.5 from 4 mice and 32 ex vivo embryos at 24 h from 4 donor mice was immediately processed for RNA sequencing library preparation using Chromium Next GEM Single Cell 3’ Library and Gel BeadKit v3.1 (PN-1000268, 10x genomics, USA), Chromium Next GEM Chip G Single Cell Kit (PN1000120, 10x genomics, USA), and Chromium iX (10x genomics, USA).



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