single-cell mouse multiplexing kit (Becton Dickinson)
90
Structured Review
Becton Dickinson
single-cell mouse multiplexing kit
Single Cell Mouse Multiplexing Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+multiplex+kit/mouse+immune+single+cell+multiplexing+kit/pmc10074823-107-15-19
Average 90 stars, based on 1 article reviews
Single Cell Mouse Multiplexing Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+multiplex+kit/mouse+immune+single+cell+multiplexing+kit/pmc10074823-107-15-19
Average 90 stars, based on 1 article reviews
single-cell mouse multiplexing kit - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Temporal tracking of microglial and monocyte single-cell transcriptomics in lethal flavivirus infection"
Article Title: Temporal tracking of microglial and monocyte single-cell transcriptomics in lethal flavivirus infection
Journal: Acta Neuropathologica Communications
doi: 10.1186/s40478-023-01547-4
Figure Legend Snippet: Single-cell RNA sequencing analysis on resident and infiltrating myeloid cells from the virus-infected CNS. a Schematic showing scRNA-seq pipeline. Microglia from mock-infected and microglia and MCs from WNV-infected brains at dpi 5 and 7 were flow cytometrically sorted, barcoded and pooled in equal numbers for cell capture on the BD Rhapsody. Libraries were sequenced on the Illumina NextSeq 1000 and processed in SevenBridges and Seurat. b UMAP plot showing 6 microglia and 3 MC clusters. c, d Percent ( c ) and number ( d ) of each microglia and MC cluster out of total microglia and MC populations in mock-infected and infected brains at dpi 5 and 7. e UMAP plot coloured by cell type and timepoint and overlaid with sample identifiers/barcode numbers. f Dot plot showing the expression of nominal microglia and MC genes in total microglia and MC populations in mock-infected and infected brains at dpi 5 and 7. g Heatmap showing the top differentially expressed genes in total microglia and MC populations in mock-infected and infected brains at dpi 5 and 7. Data is presented from one independent experiment with four mice per group
Techniques Used: RNA Sequencing Assay, Infection, Expressing
Figure Legend Snippet: Microglia and MCs differentially promote CD4 + T cell IFN-γ production and CD8 + T cell proliferation. a Summary table showing changes in the frequency of IFN-γ + and BrdU + T cells with microglia and MC depletion in WNV-infected mice. b Flow cytometric dot plots showing the expression of IFN-γ in fully-stained samples and a fluorescence-minus-one (FMO, i.e. , without anti-IFN-γ) in non-stimulated T cells from mice fed control ( Ctrl ) or PLX5622-formulated chow ( PLX ) until sacrifice at dpi 7. c , d Number and frequency IFN-γ-producing T cells, geometric mean of IFN-γ in T cells and correlation analysis between the number of microglia and frequency of IFN-γ-producing T cells from single-cell brain suspensions from Ctrl and PLX mice sacrificed at dpi 5 ( c ) or dpi 7 ( d ) . e Number and frequency IFN-γ-producing T cells, geometric mean of IFN-γ in T cells and correlation analysis between the number of Ly6C hi MCs and frequency of IFN-γ-producing T cells from single-cell brain suspensions from anti-Ly6C-treated mice sacrificed at dpi 7 ( e ). f–i Frequency of BrdU + proliferating T cells in WNV-infected mice treated with PLX5622 and culled at dpi 5 ( f ) or 7 ( g ) or in WNV-infected mice treated with monoclonal antibodies to block monocyte infiltration and culled at dpi 5 ( h ) or 7 ( i ) . Data is presented as mean ± SEM from one or two independent experiments with 3–8 mice per group
Techniques Used: Infection, Expressing, Staining, Fluorescence, Blocking Assay
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Article Title: High-Dimensional Methods of Single-Cell Microglial Profiling to Enhance Understanding of Neuropathological Disease. 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Article Title: High-Dimensional Methods of Single-Cell Microglial Profiling to Enhance Understanding of Neuropathological Disease. 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Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an AntiMouse CD45, Clone 30-F11 antibody, of the BD Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody, of the BD Expressing:Article Title: IL-33-induced keratoconjunctivitis is mediated by group 2 innate lymphoid cells in mice. Article Snippet: For the library construction, the BD Mouse Immune Single-Cell Multiplexing Kit (#633793, BD Biosciences) and the BD Rhapsody Immune Response Targeted Panel for Mouse (#633753, BD Biosciences), which consisted of primer sets for 397 genes, were used. Article Title: High-Dimensional Methods of Single-Cell Microglial Profiling to Enhance Understanding of Neuropathological Disease. Article Snippet: 1Viral Immunopathology Laboratory, Infection, Immunity and Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia 2Charles Perkins Centre, The University of Sydney, Sydney, Australia 3Cytek Biosciences, Inc., Fremont, California 4Sydney Cytometry, The University of Sydney and Centenary Institute, Sydney, Australia 5The University of Sydney Institute for Infectious Diseases, The University of Sydney, Sydney, Australia 6The University of Sydney Nano Institute, The University of Sydney, Sydney, Australia 7Corresponding author: spiteri.alanna@gmail.com Article Title: The mode of action of IL-23 in experimental inflammatory arthritic pain and disease Article Snippet: Sorted cells were labelled with sample tags using a Article Title: CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cells Article Snippet: The single cells were labeled with sample tags using the BD Article Title: Unraveling the immunological roles of serum amyloid A3 in aortic immune cell subsets during atherosclerosis progression Article Snippet: Single-cell samples were labeled using the BDTM Mouse Immune Single-Cell Multiplexing Kit (Cat. 633793) for each cartridge run on the BD Rhapsody Express Single Cell Analysis System for single-cell capture, followed by cDNA synthesis with the BD Rhapsody cDNA Kit (Cat. 633773). Article Title: Single-cell RNA sequencing reveals the dynamics and heterogeneity of lymph node immune cells during acute and chronic viral infections Article Snippet: The sorted three subsets from the same sample were sequentially labeled using BD Mouse Immune Single-Cell Multiplexing Kit conjugated to an Anti-Mouse CD45 antibody (Clone 30-F11) and BD AbSeq Ab-Oligos reagents according to the manufacturer’s protocol (BD Pharmingen). Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model. Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an AntiMouse CD45, Clone 30-F11 antibody, of the BD Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody, of the BD Flow Cytometry:Article Title: IL-33-induced keratoconjunctivitis is mediated by group 2 innate lymphoid cells in mice. Article Snippet: For the library construction, the BD Mouse Immune Single-Cell Multiplexing Kit (#633793, BD Biosciences) and the BD Rhapsody Immune Response Targeted Panel for Mouse (#633753, BD Biosciences), which consisted of primer sets for 397 genes, were used. Article Title: High-Dimensional Methods of Single-Cell Microglial Profiling to Enhance Understanding of Neuropathological Disease. Article Snippet: 1Viral Immunopathology Laboratory, Infection, Immunity and Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia 2Charles Perkins Centre, The University of Sydney, Sydney, Australia 3Cytek Biosciences, Inc., Fremont, California 4Sydney Cytometry, The University of Sydney and Centenary Institute, Sydney, Australia 5The University of Sydney Institute for Infectious Diseases, The University of Sydney, Sydney, Australia 6The University of Sydney Nano Institute, The University of Sydney, Sydney, Australia 7Corresponding author: spiteri.alanna@gmail.com Article Title: The mode of action of IL-23 in experimental inflammatory arthritic pain and disease Article Snippet: Sorted cells were labelled with sample tags using a Article Title: CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cells Article Snippet: The single cells were labeled with sample tags using the BD Article Title: Unraveling the immunological roles of serum amyloid A3 in aortic immune cell subsets during atherosclerosis progression Article Snippet: Single-cell samples were labeled using the BDTM Mouse Immune Single-Cell Multiplexing Kit (Cat. 633793) for each cartridge run on the BD Rhapsody Express Single Cell Analysis System for single-cell capture, followed by cDNA synthesis with the BD Rhapsody cDNA Kit (Cat. 633773). Article Title: Single-cell RNA sequencing reveals the dynamics and heterogeneity of lymph node immune cells during acute and chronic viral infections Article Snippet: The sorted three subsets from the same sample were sequentially labeled using BD Mouse Immune Single-Cell Multiplexing Kit conjugated to an Anti-Mouse CD45 antibody (Clone 30-F11) and BD AbSeq Ab-Oligos reagents according to the manufacturer’s protocol (BD Pharmingen). Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model. Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an AntiMouse CD45, Clone 30-F11 antibody, of the BD Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody, of the BD Infection:Article Title: IL-33-induced keratoconjunctivitis is mediated by group 2 innate lymphoid cells in mice. Article Snippet: For the library construction, the BD Mouse Immune Single-Cell Multiplexing Kit (#633793, BD Biosciences) and the BD Rhapsody Immune Response Targeted Panel for Mouse (#633753, BD Biosciences), which consisted of primer sets for 397 genes, were used. Article Title: High-Dimensional Methods of Single-Cell Microglial Profiling to Enhance Understanding of Neuropathological Disease. Article Snippet: 1Viral Immunopathology Laboratory, Infection, Immunity and Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia 2Charles Perkins Centre, The University of Sydney, Sydney, Australia 3Cytek Biosciences, Inc., Fremont, California 4Sydney Cytometry, The University of Sydney and Centenary Institute, Sydney, Australia 5The University of Sydney Institute for Infectious Diseases, The University of Sydney, Sydney, Australia 6The University of Sydney Nano Institute, The University of Sydney, Sydney, Australia 7Corresponding author: spiteri.alanna@gmail.com Article Title: The mode of action of IL-23 in experimental inflammatory arthritic pain and disease Article Snippet: Sorted cells were labelled with sample tags using a Article Title: CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cells Article Snippet: The single cells were labeled with sample tags using the BD Article Title: Unraveling the immunological roles of serum amyloid A3 in aortic immune cell subsets during atherosclerosis progression Article Snippet: Single-cell samples were labeled using the BDTM Mouse Immune Single-Cell Multiplexing Kit (Cat. 633793) for each cartridge run on the BD Rhapsody Express Single Cell Analysis System for single-cell capture, followed by cDNA synthesis with the BD Rhapsody cDNA Kit (Cat. 633773). Article Title: Single-cell RNA sequencing reveals the dynamics and heterogeneity of lymph node immune cells during acute and chronic viral infections Article Snippet: The sorted three subsets from the same sample were sequentially labeled using BD Mouse Immune Single-Cell Multiplexing Kit conjugated to an Anti-Mouse CD45 antibody (Clone 30-F11) and BD AbSeq Ab-Oligos reagents according to the manufacturer’s protocol (BD Pharmingen). Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model. Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an AntiMouse CD45, Clone 30-F11 antibody, of the BD Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody, of the BD Virus:Article Title: IL-33-induced keratoconjunctivitis is mediated by group 2 innate lymphoid cells in mice. Article Snippet: For the library construction, the BD Mouse Immune Single-Cell Multiplexing Kit (#633793, BD Biosciences) and the BD Rhapsody Immune Response Targeted Panel for Mouse (#633753, BD Biosciences), which consisted of primer sets for 397 genes, were used. Article Title: High-Dimensional Methods of Single-Cell Microglial Profiling to Enhance Understanding of Neuropathological Disease. Article Snippet: 1Viral Immunopathology Laboratory, Infection, Immunity and Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia 2Charles Perkins Centre, The University of Sydney, Sydney, Australia 3Cytek Biosciences, Inc., Fremont, California 4Sydney Cytometry, The University of Sydney and Centenary Institute, Sydney, Australia 5The University of Sydney Institute for Infectious Diseases, The University of Sydney, Sydney, Australia 6The University of Sydney Nano Institute, The University of Sydney, Sydney, Australia 7Corresponding author: spiteri.alanna@gmail.com Article Title: The mode of action of IL-23 in experimental inflammatory arthritic pain and disease Article Snippet: Sorted cells were labelled with sample tags using a Article Title: CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cells Article Snippet: The single cells were labeled with sample tags using the BD Article Title: Unraveling the immunological roles of serum amyloid A3 in aortic immune cell subsets during atherosclerosis progression Article Snippet: Single-cell samples were labeled using the BDTM Mouse Immune Single-Cell Multiplexing Kit (Cat. 633793) for each cartridge run on the BD Rhapsody Express Single Cell Analysis System for single-cell capture, followed by cDNA synthesis with the BD Rhapsody cDNA Kit (Cat. 633773). Article Title: Single-cell RNA sequencing reveals the dynamics and heterogeneity of lymph node immune cells during acute and chronic viral infections Article Snippet: The sorted three subsets from the same sample were sequentially labeled using BD Mouse Immune Single-Cell Multiplexing Kit conjugated to an Anti-Mouse CD45 antibody (Clone 30-F11) and BD AbSeq Ab-Oligos reagents according to the manufacturer’s protocol (BD Pharmingen). Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model. Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an AntiMouse CD45, Clone 30-F11 antibody, of the BD Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody, of the BD Suspension:Article Title: IL-33-induced keratoconjunctivitis is mediated by group 2 innate lymphoid cells in mice. Article Snippet: For the library construction, the BD Mouse Immune Single-Cell Multiplexing Kit (#633793, BD Biosciences) and the BD Rhapsody Immune Response Targeted Panel for Mouse (#633753, BD Biosciences), which consisted of primer sets for 397 genes, were used. Article Title: High-Dimensional Methods of Single-Cell Microglial Profiling to Enhance Understanding of Neuropathological Disease. Article Snippet: 1Viral Immunopathology Laboratory, Infection, Immunity and Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia 2Charles Perkins Centre, The University of Sydney, Sydney, Australia 3Cytek Biosciences, Inc., Fremont, California 4Sydney Cytometry, The University of Sydney and Centenary Institute, Sydney, Australia 5The University of Sydney Institute for Infectious Diseases, The University of Sydney, Sydney, Australia 6The University of Sydney Nano Institute, The University of Sydney, Sydney, Australia 7Corresponding author: spiteri.alanna@gmail.com Article Title: The mode of action of IL-23 in experimental inflammatory arthritic pain and disease Article Snippet: Sorted cells were labelled with sample tags using a Article Title: CXXC-finger protein 1 associates with FOXP3 to stabilize homeostasis and suppressive functions of regulatory T cells Article Snippet: The single cells were labeled with sample tags using the BD Article Title: Unraveling the immunological roles of serum amyloid A3 in aortic immune cell subsets during atherosclerosis progression Article Snippet: Single-cell samples were labeled using the BDTM Mouse Immune Single-Cell Multiplexing Kit (Cat. 633793) for each cartridge run on the BD Rhapsody Express Single Cell Analysis System for single-cell capture, followed by cDNA synthesis with the BD Rhapsody cDNA Kit (Cat. 633773). Article Title: Single-cell RNA sequencing reveals the dynamics and heterogeneity of lymph node immune cells during acute and chronic viral infections Article Snippet: The sorted three subsets from the same sample were sequentially labeled using BD Mouse Immune Single-Cell Multiplexing Kit conjugated to an Anti-Mouse CD45 antibody (Clone 30-F11) and BD AbSeq Ab-Oligos reagents according to the manufacturer’s protocol (BD Pharmingen). Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model. Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an AntiMouse CD45, Clone 30-F11 antibody, of the BD Article Title: Single-Cell RNA Sequencing Reveals Immuno-Oncology Characteristics of Tumor-Infiltrating T Lymphocytes in Photodynamic Therapy-Treated Colorectal Cancer Mouse Model Article Snippet: The prepared single-cell suspension was labeled in a Sample Tag tube, which had a sample oligo barcode conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody, of the BD |
