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mouse cytokine detecting elisa kits  (Boster Bio)


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    Boster Bio mouse cytokine detecting elisa kits
    Effect of AJSAF on H9–Ag-specific IgG and its isotype antibody titers in the immunized mice. Mice were s.c. injected with 0.2 ml of IH9V (10 7 TCID 50 /0.1 ml) alone or containing Quil A or AJSAF, or 0.2 ml of CH9V (10 7 TCID 50 /0.1 ml) on days 1 and 15. Sera were collected 14 days after the boosting immunization, and serum H9–Ag-specific IgG, IgG1, IgG2a, and IgG2b antibody titers were measured by <t>ELISA.</t> The values are presented as means ± SD ( n = 6). Significant differences with IH9V alone group were designated as * P < 0.05, † P < 0.01, and ‡ P < 0.001; those with CH9V group as § P < 0.05, # P < 0.01, and ¶ P < 0.001
    Mouse Cytokine Detecting Elisa Kits, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+multiplex+kit/Multiplex+ELISA+Kit+For+Mouse+Cytokine+-+Screen/pmc07648552-112-17-24
    Average 93 stars, based on 11 article reviews
    mouse cytokine detecting elisa kits - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Quick and improved immune responses to inactivated H9N2 avian influenza vaccine by purified active fraction of Albizia julibrissin saponins"

    Article Title: Quick and improved immune responses to inactivated H9N2 avian influenza vaccine by purified active fraction of Albizia julibrissin saponins

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-020-02648-1

    Effect of AJSAF on H9–Ag-specific IgG and its isotype antibody titers in the immunized mice. Mice were s.c. injected with 0.2 ml of IH9V (10 7 TCID 50 /0.1 ml) alone or containing Quil A or AJSAF, or 0.2 ml of CH9V (10 7 TCID 50 /0.1 ml) on days 1 and 15. Sera were collected 14 days after the boosting immunization, and serum H9–Ag-specific IgG, IgG1, IgG2a, and IgG2b antibody titers were measured by ELISA. The values are presented as means ± SD ( n = 6). Significant differences with IH9V alone group were designated as * P < 0.05, † P < 0.01, and ‡ P < 0.001; those with CH9V group as § P < 0.05, # P < 0.01, and ¶ P < 0.001
    Figure Legend Snippet: Effect of AJSAF on H9–Ag-specific IgG and its isotype antibody titers in the immunized mice. Mice were s.c. injected with 0.2 ml of IH9V (10 7 TCID 50 /0.1 ml) alone or containing Quil A or AJSAF, or 0.2 ml of CH9V (10 7 TCID 50 /0.1 ml) on days 1 and 15. Sera were collected 14 days after the boosting immunization, and serum H9–Ag-specific IgG, IgG1, IgG2a, and IgG2b antibody titers were measured by ELISA. The values are presented as means ± SD ( n = 6). Significant differences with IH9V alone group were designated as * P < 0.05, † P < 0.01, and ‡ P < 0.001; those with CH9V group as § P < 0.05, # P < 0.01, and ¶ P < 0.001

    Techniques Used: Injection, Enzyme-linked Immunosorbent Assay

    Effect of AJSAF on haemagglutination inhibition (HI) and H9–Ag-specific IgY antibody levels in the immunized chicken. The chickens were prime- and boost-immunized with 0.4 ml of IH9V (10 7 TCID 50 /0.1 ml) alone or containing AJSAF, or 0.4 ml of CH9V (10 7 TCID 50 /0.1 ml) at 3-week interval. Sera were collected on designated days post-immunization, and the serum HI antibody titers ( a ) and H9–Ag-specific IgY antibody ( b ) levels were measured by HI assay and ELISA, respectively. The values are presented as means ± SD ( n = 30). Significant differences with IH9V alone group were designated as * P < 0.05, † P < 0.01, and ‡ P < 0.001
    Figure Legend Snippet: Effect of AJSAF on haemagglutination inhibition (HI) and H9–Ag-specific IgY antibody levels in the immunized chicken. The chickens were prime- and boost-immunized with 0.4 ml of IH9V (10 7 TCID 50 /0.1 ml) alone or containing AJSAF, or 0.4 ml of CH9V (10 7 TCID 50 /0.1 ml) at 3-week interval. Sera were collected on designated days post-immunization, and the serum HI antibody titers ( a ) and H9–Ag-specific IgY antibody ( b ) levels were measured by HI assay and ELISA, respectively. The values are presented as means ± SD ( n = 30). Significant differences with IH9V alone group were designated as * P < 0.05, † P < 0.01, and ‡ P < 0.001

    Techniques Used: Inhibition, HI Assay, Enzyme-linked Immunosorbent Assay

    Effect of AJSAF on cytokine secretion from splenocytes in the immunized mice. Splenocytes were incubated with Con A (48 h) or H9–Ag (72 h), and the supernatants were collected for detecting IL-2, IFN-γ, and IL-10 levels using ELISA kits. The values are presented as means ± SD ( n = 6). Significant differences with IH9V alone group were designated as * P < 0.05, † P < 0.01, and ‡ P < 0.001; those with CH9V group as # P < 0.01 and ¶ P < 0.001
    Figure Legend Snippet: Effect of AJSAF on cytokine secretion from splenocytes in the immunized mice. Splenocytes were incubated with Con A (48 h) or H9–Ag (72 h), and the supernatants were collected for detecting IL-2, IFN-γ, and IL-10 levels using ELISA kits. The values are presented as means ± SD ( n = 6). Significant differences with IH9V alone group were designated as * P < 0.05, † P < 0.01, and ‡ P < 0.001; those with CH9V group as # P < 0.01 and ¶ P < 0.001

    Techniques Used: Incubation, Enzyme-linked Immunosorbent Assay

    Related Articles

    Multiplex Assay:

    Article Title: Therapeutic Effect and Immune Changes after Treatment of Hymenolepis nana -Infected BALB/c Mice with Compounds Isolated from Leucaena leucocephala
    Article Snippet: Concanavalin A (con A) (L7647) and dimethyl sulfoxide (DMSO) (D2650) were obtained from the Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). .. The Multiplex ELISA Kit For Mouse Cytokine (MEK1006, GMCSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, MCP-1, MIP-1α, RANTES, TNFα) was obtained from Boster Biological Technology (Pleasanton, CA, USA). .. Certified fetal bovine serum (FBS) (04-001-1A) was obtained from Biological Industries (Kibbutz Beit Haemek, Israel).

    Article Title: Electronic Cigarettes Induce Mitochondrial DNA Damage and Trigger TLR9 (Toll-Like Receptor 9)-Mediated Atherosclerosis.
    Article Snippet: .. Multiplex ELISA for mouse cytokines Aliquots of plasma from mice were analyzed using mouse cytokine 9-Plex ELISA kit from Boster Biological Technology (Pleasanton, CA) according to manufacturer’s instructions. .. Histological and immunohistochemical studies The formalin fixed heart and whole aorta were dehydrated in ethanol and embedded in paraffin, and 5-μm serial sections were stained with hematoxylin and eosin (Millipore Sigma, Burlington, MA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Therapeutic Effect and Immune Changes after Treatment of Hymenolepis nana -Infected BALB/c Mice with Compounds Isolated from Leucaena leucocephala
    Article Snippet: Concanavalin A (con A) (L7647) and dimethyl sulfoxide (DMSO) (D2650) were obtained from the Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). .. The Multiplex ELISA Kit For Mouse Cytokine (MEK1006, GMCSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, MCP-1, MIP-1α, RANTES, TNFα) was obtained from Boster Biological Technology (Pleasanton, CA, USA). .. Certified fetal bovine serum (FBS) (04-001-1A) was obtained from Biological Industries (Kibbutz Beit Haemek, Israel).

    Article Title: Quick and improved immune responses to inactivated H9N2 avian influenza vaccine by purified active fraction of Albizia julibrissin saponins
    Article Snippet: Concanavalin A (Con A), lipopolysaccharide (LPS), rabbit anti-mouse IgG peroxidase conjugate, RPMI medium, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) were purchased from Sigma Co., St. Louis, MO, USA; fetal calf serum was from Hyclone, Utah, USA; goat anti-mouse IgG1, IgG2a, and IgG2b peroxidase-conjugates were from Southern Biotech. .. Assoc., Birmingham, AL, USA; rabbit anti-chicken IgY horseradish peroxidase conjugate was from Promega Corporation, Madison, WI, USA; mouse cytokine detecting ELISA kits were from Wuhan Boster Bio-Tech. .. Co. Ltd., China; Trizol was from Invitrogen, Carlsbad, CA, USA; FastStart universal SYBR Green Master (ROX) was from Roche Diagnostics, Indianapolis, IN, USA.

    Article Title: Hybrids of aurantiamide acetate and isopropylated genipin as potential anti-inflammatory agents: The design, synthesis, and biological evaluation.
    Article Snippet: A novel series of hybrids designed on the basis of aurantiamide acetate and isopropylated A cc ep te d A rt ic le

    Article Title: Penicilazaphilone C alleviates allergic airway inflammation and improves the immune microenvironment by hindering the NLRP3 inflammasome.
    Article Snippet: .. In order to assess the levels of inflammatory cytokines such as IL-4, IL-5, IL-13, IL-1β, and IL-18 in BALF, as well as the quantity of OVAspecific IgE in serum, we utilized an enzyme-linked immunosorbent assay (ELISA) with ELISA kits obtained from Boster Biotechnology (MEK1006, Wuhan, China). ..

    Article Title: Electronic Cigarettes Induce Mitochondrial DNA Damage and Trigger TLR9 (Toll-Like Receptor 9)-Mediated Atherosclerosis.
    Article Snippet: .. Multiplex ELISA for mouse cytokines Aliquots of plasma from mice were analyzed using mouse cytokine 9-Plex ELISA kit from Boster Biological Technology (Pleasanton, CA) according to manufacturer’s instructions. .. Histological and immunohistochemical studies The formalin fixed heart and whole aorta were dehydrated in ethanol and embedded in paraffin, and 5-μm serial sections were stained with hematoxylin and eosin (Millipore Sigma, Burlington, MA).

    Clinical Proteomics:

    Article Title: Electronic Cigarettes Induce Mitochondrial DNA Damage and Trigger TLR9 (Toll-Like Receptor 9)-Mediated Atherosclerosis.
    Article Snippet: .. Multiplex ELISA for mouse cytokines Aliquots of plasma from mice were analyzed using mouse cytokine 9-Plex ELISA kit from Boster Biological Technology (Pleasanton, CA) according to manufacturer’s instructions. .. Histological and immunohistochemical studies The formalin fixed heart and whole aorta were dehydrated in ethanol and embedded in paraffin, and 5-μm serial sections were stained with hematoxylin and eosin (Millipore Sigma, Burlington, MA).



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    Transcriptomic profiling and cytokine production in CIRBP-stimulated primary mouse lung fibroblasts. ( A ) Volcano plot of differentially expressed genes in fibroblasts treated with rmCIRBP vs. control. ( B ) Heatmap of representative upregulated and downregulated genes (fold change > 2.5). ( C , D ) Quantitative PCR analysis of Acta2 and Fn1 mRNA expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( E , F ) Western blot analysis of α-SMA and fibronectin protein expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( G – L ) <t>ELISA</t> quantification of cytokines in the culture supernatants: TGF-β, PDGF, IFN-γ, IL-1β, TNF-α, and IL-6. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test. rmCIRBP recombinant mouse CIRBP.
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    Transcriptomic profiling and cytokine production in CIRBP-stimulated primary mouse lung fibroblasts. ( A ) Volcano plot of differentially expressed genes in fibroblasts treated with rmCIRBP vs. control. ( B ) Heatmap of representative upregulated and downregulated genes (fold change > 2.5). ( C , D ) Quantitative PCR analysis of Acta2 and Fn1 mRNA expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( E , F ) Western blot analysis of α-SMA and fibronectin protein expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( G – L ) <t>ELISA</t> quantification of cytokines in the culture supernatants: TGF-β, PDGF, IFN-γ, IL-1β, TNF-α, and IL-6. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test. rmCIRBP recombinant mouse CIRBP.
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    Image Search Results


    Transcriptomic profiling and cytokine production in CIRBP-stimulated primary mouse lung fibroblasts. ( A ) Volcano plot of differentially expressed genes in fibroblasts treated with rmCIRBP vs. control. ( B ) Heatmap of representative upregulated and downregulated genes (fold change > 2.5). ( C , D ) Quantitative PCR analysis of Acta2 and Fn1 mRNA expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( E , F ) Western blot analysis of α-SMA and fibronectin protein expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( G – L ) ELISA quantification of cytokines in the culture supernatants: TGF-β, PDGF, IFN-γ, IL-1β, TNF-α, and IL-6. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test. rmCIRBP recombinant mouse CIRBP.

    Journal: Scientific Reports

    Article Title: Cold inducible RNA binding protein promotes fibroblast activation and its inhibition represents a potential therapeutic target in pulmonary fibrosis

    doi: 10.1038/s41598-026-39649-3

    Figure Lengend Snippet: Transcriptomic profiling and cytokine production in CIRBP-stimulated primary mouse lung fibroblasts. ( A ) Volcano plot of differentially expressed genes in fibroblasts treated with rmCIRBP vs. control. ( B ) Heatmap of representative upregulated and downregulated genes (fold change > 2.5). ( C , D ) Quantitative PCR analysis of Acta2 and Fn1 mRNA expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( E , F ) Western blot analysis of α-SMA and fibronectin protein expression in fibroblasts treated with or without rmCIRBP. TGF-β–stimulated fibroblasts were included as a positive control. ( G – L ) ELISA quantification of cytokines in the culture supernatants: TGF-β, PDGF, IFN-γ, IL-1β, TNF-α, and IL-6. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test. rmCIRBP recombinant mouse CIRBP.

    Article Snippet: After 24 h of treatment, cytokine concentrations in the culture supernatants were quantified using the arigoPLEX® Mouse Proinflammatory Cytokine Multiplex ELISA Kit and the arigoPLEX® Mouse Fibrotic Marker Multiplex ELISA Kit (arigo Biolaboratories Corp., Taiwan) according to the manufacturer’s instructions.

    Techniques: Control, Real-time Polymerase Chain Reaction, Expressing, Positive Control, Western Blot, Enzyme-linked Immunosorbent Assay, Comparison, Recombinant

    CIRBP promotes collagen production and proliferation via TLR2/4-dependent IL-6 signaling. ( A ) IL-6 concentrations in the culture supernatants of primary lung fibroblasts treated with rmCIRBP, with or without C23, TLR4-I, or TLR2-I, measured by ELISA. ( B ) Soluble collagen levels in the supernatants following treatment with rmCIRBP ± anti-IL-6 antibody. ( C ) Proliferative activity of fibroblasts assessed by the WST assay after treatment with rmCIRBP and/or anti-IL-6 antibody. ( D ) Soluble collagen production in fibroblasts treated with rmCIRBP and either TLR4-I or TLR2-I. ( E ) Fibroblast proliferation measured using the WST assay following cotreatment with rmCIRBP and TLR4-I or TLR2-I. ( F ) Schematic representation of the proposed mechanism: CIRBP, which is highly expressed in fibrotic lungs, stimulates IL-6 production via TLR2/4, promoting collagen secretion and fibroblast proliferation in an autocrine manner and contributing to the progression of tissue fibrosis. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test.

    Journal: Scientific Reports

    Article Title: Cold inducible RNA binding protein promotes fibroblast activation and its inhibition represents a potential therapeutic target in pulmonary fibrosis

    doi: 10.1038/s41598-026-39649-3

    Figure Lengend Snippet: CIRBP promotes collagen production and proliferation via TLR2/4-dependent IL-6 signaling. ( A ) IL-6 concentrations in the culture supernatants of primary lung fibroblasts treated with rmCIRBP, with or without C23, TLR4-I, or TLR2-I, measured by ELISA. ( B ) Soluble collagen levels in the supernatants following treatment with rmCIRBP ± anti-IL-6 antibody. ( C ) Proliferative activity of fibroblasts assessed by the WST assay after treatment with rmCIRBP and/or anti-IL-6 antibody. ( D ) Soluble collagen production in fibroblasts treated with rmCIRBP and either TLR4-I or TLR2-I. ( E ) Fibroblast proliferation measured using the WST assay following cotreatment with rmCIRBP and TLR4-I or TLR2-I. ( F ) Schematic representation of the proposed mechanism: CIRBP, which is highly expressed in fibrotic lungs, stimulates IL-6 production via TLR2/4, promoting collagen secretion and fibroblast proliferation in an autocrine manner and contributing to the progression of tissue fibrosis. Data are presented as mean ± SEM. *P < 0.05 using one-way ANOVA and Tukey’s multiple comparison test.

    Article Snippet: After 24 h of treatment, cytokine concentrations in the culture supernatants were quantified using the arigoPLEX® Mouse Proinflammatory Cytokine Multiplex ELISA Kit and the arigoPLEX® Mouse Fibrotic Marker Multiplex ELISA Kit (arigo Biolaboratories Corp., Taiwan) according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay, WST Assay, Comparison