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Arraystar inc mouse lncrna microarray v4
Establishment of cardiac failure model and identification of altered lncRNAs. A–C . Echocardiography and quantitative analysis ( n = 6–7). D–E . Representative images of hematoxylin and eosin (H&E) staining (upper) and wheat germ agglutinin (WGA) staining ( n = 6–7). Scale bar: 100 μm. F . Quantitative analysis of the heart weight (HW)/body weight (BW) ratio ( n = 6–7). G . qPCR analysis of ANP and BNP mRNA expressions ( n = 6–7). H . Heat map of the top 20 altered lncRNAs in the heart. The lncRNAs with raw intensities over 20 in the <t>microarray</t> sequencing report are framed in red. I . qPCR analysis of lncRNAs in the samples applied for microarray sequencing ( n = 3). J . qPCR validation of <t>LncRNA</t> AK144717 and Gm4316 (ENSMUST00000212411) in all the mice of sham and TAC group ( n = 6–7). K. qPCR analysis of LncRNA AK144717 expression in the TAC models of different time ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; NS means not significant between groups
Mouse Lncrna Microarray V4, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Long noncoding RNA AK144717 exacerbates pathological cardiac hypertrophy through modulating the cellular distribution of HMGB1 and subsequent DNA damage response"

Article Title: Long noncoding RNA AK144717 exacerbates pathological cardiac hypertrophy through modulating the cellular distribution of HMGB1 and subsequent DNA damage response

Journal: Cellular and Molecular Life Sciences: CMLS

doi: 10.1007/s00018-024-05464-0

Establishment of cardiac failure model and identification of altered lncRNAs. A–C . Echocardiography and quantitative analysis ( n = 6–7). D–E . Representative images of hematoxylin and eosin (H&E) staining (upper) and wheat germ agglutinin (WGA) staining ( n = 6–7). Scale bar: 100 μm. F . Quantitative analysis of the heart weight (HW)/body weight (BW) ratio ( n = 6–7). G . qPCR analysis of ANP and BNP mRNA expressions ( n = 6–7). H . Heat map of the top 20 altered lncRNAs in the heart. The lncRNAs with raw intensities over 20 in the microarray sequencing report are framed in red. I . qPCR analysis of lncRNAs in the samples applied for microarray sequencing ( n = 3). J . qPCR validation of LncRNA AK144717 and Gm4316 (ENSMUST00000212411) in all the mice of sham and TAC group ( n = 6–7). K. qPCR analysis of LncRNA AK144717 expression in the TAC models of different time ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; NS means not significant between groups
Figure Legend Snippet: Establishment of cardiac failure model and identification of altered lncRNAs. A–C . Echocardiography and quantitative analysis ( n = 6–7). D–E . Representative images of hematoxylin and eosin (H&E) staining (upper) and wheat germ agglutinin (WGA) staining ( n = 6–7). Scale bar: 100 μm. F . Quantitative analysis of the heart weight (HW)/body weight (BW) ratio ( n = 6–7). G . qPCR analysis of ANP and BNP mRNA expressions ( n = 6–7). H . Heat map of the top 20 altered lncRNAs in the heart. The lncRNAs with raw intensities over 20 in the microarray sequencing report are framed in red. I . qPCR analysis of lncRNAs in the samples applied for microarray sequencing ( n = 3). J . qPCR validation of LncRNA AK144717 and Gm4316 (ENSMUST00000212411) in all the mice of sham and TAC group ( n = 6–7). K. qPCR analysis of LncRNA AK144717 expression in the TAC models of different time ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; NS means not significant between groups

Techniques Used: Staining, Microarray, Sequencing, Biomarker Discovery, Expressing

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Article Title: Genetic inactivation of the Translin/Trax RNase activity alters small RNAs including miRNAs, disrupts gene expression and impairs distinct forms of hippocampal synaptic plasticity and memory
Article Snippet: The Arraystar Mouse Small RNA Array V1.0 comprises a total of 14,192 distinct probes and includes the following coverage for different small RNA species: miRNAs – 1,949 (966 5-p and 983 3-p); pre-miRNAs – 1,122; tsRNAs – 1,767; mature tRNAs – 270; snoRNAs – 1,324.

Article Title: Targeting Ischemic Myocardium: Nanoparticles Loaded with Long Noncoding RNA AK156373 siRNA Alleviate Myocardial Infarction.
Article Snippet: Despite advancements in the development of targeted approaches for the treatment of myocardial infarction (MI), there is a continuing need for improvements in treatment approaches due to the high mortality and prevalence of MI.. The identification of specific therapeutic targets and the development of efficient delivery systems are essential.. In this study, a nanoparticle delivery system targeting necrotic cardiomyocytes was engineered.

Article Title: Gut Microbiota–Mediated hsa_circ_0126925 Targets BCAA Metabolic Enzyme BCAT2 to Exacerbate Colorectal Cancer Progression
Article Snippet: Then, the labeled circRNA was hybridized onto an Arraystar Mouse circRNA Array (8 × 15 K, Arraystar) and incubated in an Agilent Hybridization Incubator (Agilent) at 65°C for 17 hours.

Article Title: Long Non-Coding RNA Osr2 Promotes Fusarium solani Keratitis Inflammation via the miR-30a-3p/ Xcr1 Axis.
Article Snippet: Subsequently, each RNA sample was amplified and transcribed into fluorescent complementary RNA (cRNA), which was then hybridized to the Arraystar Mouse LncRNA Microarray version 4.0.

Article Title: Exploring Mechanisms of Ephx2 in Treating Atherosclerosis Using Independent Cascade Model and Adverse Outcome Pathways.
Article Snippet: Background: Atherosclerosis (AS) is a leading cause of cardiovascular diseases, characterized by lipid accumulation in arterial walls.. The enzyme Ephx2 (soluble epoxide hydrolase, sEH) is implicated in AS development, but its precise mechanisms and therapeutic potential are not fully understood.. Objectives: This study aimed to analyze gene expression data from low-density lipoprotein receptor knockout (LDLR88) and LDLR88sEH88 mice to identify significant genes associated with

Labeling:

Article Title: Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis.
Article Snippet: Then, the enriched circRNAs were amplified and transcribed into fluorescent cRNA using a random priming method (Arraystar Super RNA Labeling Kit; Arraystar). .. The labeled cRNAs were hybridized onto the Arraystar Mouse circRNA Microarray V2. .. After the slides were washed, the arrays were scanned by the Agilent Scanner G2505C.

Article Title: Characterization of circRNA expression profiles and functional roles in a mouse model of liver injury induced by OSA.
Article Snippet: .. Arraystar Mouse circRNA Array V2 was hybridized with the labeled cRNAs, which were subsequently incubated at 65 °C for 17 h. Following the washing of the slides, we scanned the array using the Agilent Scanner G2505C. .. The resulting array images were analyzed using Agilent Feature Extraction Software.

Microarray:

Article Title: Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis.
Article Snippet: Then, the enriched circRNAs were amplified and transcribed into fluorescent cRNA using a random priming method (Arraystar Super RNA Labeling Kit; Arraystar). .. The labeled cRNAs were hybridized onto the Arraystar Mouse circRNA Microarray V2. .. After the slides were washed, the arrays were scanned by the Agilent Scanner G2505C.

Incubation:

Article Title: Characterization of circRNA expression profiles and functional roles in a mouse model of liver injury induced by OSA.
Article Snippet: .. Arraystar Mouse circRNA Array V2 was hybridized with the labeled cRNAs, which were subsequently incubated at 65 °C for 17 h. Following the washing of the slides, we scanned the array using the Agilent Scanner G2505C. .. The resulting array images were analyzed using Agilent Feature Extraction Software.



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Pipeline of the experimental approach followed. Female ApoE -/- mice were treated twice weekly for 16 weeks (arrowheads) with an intraperitoneal administration of 50 μg of an αCD40 siRNA (treatment group, T) or 50 μg of a scrambled sequence siRNA (control group, C). Aortic tissue was extracted at weeks 8 (basal) and 10 and 24 for both experimental groups (C10, C24, T10 and T24). Total RNA was extracted and used for a <t>microarray</t> experiment in which expression data were normalized to the basal levels at week 8. Only downregulated transcripts of the C and T groups were used in this analysis of length dynamics (see text). In parenthesis is the number of transcripts from each group. Transcripts simultaneously expressed in two experimental conditions (C10/C24 and T10/T24) were identified and used for bioinformatic analysis of the mechanisms regulating transcript length variation.
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Establishment of cardiac failure model and identification of altered lncRNAs. A–C . Echocardiography and quantitative analysis ( n = 6–7). D–E . Representative images of hematoxylin and eosin (H&E) staining (upper) and wheat germ agglutinin (WGA) staining ( n = 6–7). Scale bar: 100 μm. F . Quantitative analysis of the heart weight (HW)/body weight (BW) ratio ( n = 6–7). G . qPCR analysis of ANP and BNP mRNA expressions ( n = 6–7). H . Heat map of the top 20 altered lncRNAs in the heart. The lncRNAs with raw intensities over 20 in the <t>microarray</t> sequencing report are framed in red. I . qPCR analysis of lncRNAs in the samples applied for microarray sequencing ( n = 3). J . qPCR validation of <t>LncRNA</t> AK144717 and Gm4316 (ENSMUST00000212411) in all the mice of sham and TAC group ( n = 6–7). K. qPCR analysis of LncRNA AK144717 expression in the TAC models of different time ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; NS means not significant between groups
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Establishment of cardiac failure model and identification of altered lncRNAs. A–C . Echocardiography and quantitative analysis ( n = 6–7). D–E . Representative images of hematoxylin and eosin (H&E) staining (upper) and wheat germ agglutinin (WGA) staining ( n = 6–7). Scale bar: 100 μm. F . Quantitative analysis of the heart weight (HW)/body weight (BW) ratio ( n = 6–7). G . qPCR analysis of ANP and BNP mRNA expressions ( n = 6–7). H . Heat map of the top 20 altered lncRNAs in the heart. The lncRNAs with raw intensities over 20 in the <t>microarray</t> sequencing report are framed in red. I . qPCR analysis of lncRNAs in the samples applied for microarray sequencing ( n = 3). J . qPCR validation of <t>LncRNA</t> AK144717 and Gm4316 (ENSMUST00000212411) in all the mice of sham and TAC group ( n = 6–7). K. qPCR analysis of LncRNA AK144717 expression in the TAC models of different time ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; NS means not significant between groups
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Image Search Results


Main features of studies with omics application in rodent ischemia and reperfusion injury model

Journal: International Journal of Biological Sciences

Article Title: Molecular Mechanisms of Ischemia/Reperfusion Injury and Graft Dysfunction in Liver Transplantation: Insights from Multi-Omics Studies in Rodent Animal Models

doi: 10.7150/ijbs.109449

Figure Lengend Snippet: Main features of studies with omics application in rodent ischemia and reperfusion injury model

Article Snippet: Hua, China, 2022 , C57BL6 mice/ male/ 8-10 wks/ 6 vs. 6 , 70% WI (left+middle lobe) , IPC/none , Liver/at the end of each treatment , MicroArray/Arraystar Mouse LncRNA Microarray V3.0 , mRNA/ lncRNA/ transcriptomics , 1.IPC+I/R vs. I/R , One-way ANOVA using Dunnett t-test , 167 DE lncRNAs and 108 mRNAs were identified in IPC+I/R groups. KEGG analysis indicated DEGs were enriched in protein processing in the endoplasmic reticulum, antigen processing and presentation, and fructose and mannose metabolism. , Tissue qRT-PCR , GSE192977.

Techniques: Control, Sampling, Comparison, Biomarker Discovery, Microarray, Ubiquitin Proteomics, Functional Assay, Expressing, Protein-Protein interactions, Gene Expression, Migration, Activation Assay, TUNEL Assay, Infection, Construct, Sequencing

Pipeline of the experimental approach followed. Female ApoE -/- mice were treated twice weekly for 16 weeks (arrowheads) with an intraperitoneal administration of 50 μg of an αCD40 siRNA (treatment group, T) or 50 μg of a scrambled sequence siRNA (control group, C). Aortic tissue was extracted at weeks 8 (basal) and 10 and 24 for both experimental groups (C10, C24, T10 and T24). Total RNA was extracted and used for a microarray experiment in which expression data were normalized to the basal levels at week 8. Only downregulated transcripts of the C and T groups were used in this analysis of length dynamics (see text). In parenthesis is the number of transcripts from each group. Transcripts simultaneously expressed in two experimental conditions (C10/C24 and T10/T24) were identified and used for bioinformatic analysis of the mechanisms regulating transcript length variation.

Journal: Biomedicines

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice

doi: 10.3390/biomedicines12122703

Figure Lengend Snippet: Pipeline of the experimental approach followed. Female ApoE -/- mice were treated twice weekly for 16 weeks (arrowheads) with an intraperitoneal administration of 50 μg of an αCD40 siRNA (treatment group, T) or 50 μg of a scrambled sequence siRNA (control group, C). Aortic tissue was extracted at weeks 8 (basal) and 10 and 24 for both experimental groups (C10, C24, T10 and T24). Total RNA was extracted and used for a microarray experiment in which expression data were normalized to the basal levels at week 8. Only downregulated transcripts of the C and T groups were used in this analysis of length dynamics (see text). In parenthesis is the number of transcripts from each group. Transcripts simultaneously expressed in two experimental conditions (C10/C24 and T10/T24) were identified and used for bioinformatic analysis of the mechanisms regulating transcript length variation.

Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Techniques: Sequencing, Control, Microarray, Expressing

Establishment of cardiac failure model and identification of altered lncRNAs. A–C . Echocardiography and quantitative analysis ( n = 6–7). D–E . Representative images of hematoxylin and eosin (H&E) staining (upper) and wheat germ agglutinin (WGA) staining ( n = 6–7). Scale bar: 100 μm. F . Quantitative analysis of the heart weight (HW)/body weight (BW) ratio ( n = 6–7). G . qPCR analysis of ANP and BNP mRNA expressions ( n = 6–7). H . Heat map of the top 20 altered lncRNAs in the heart. The lncRNAs with raw intensities over 20 in the microarray sequencing report are framed in red. I . qPCR analysis of lncRNAs in the samples applied for microarray sequencing ( n = 3). J . qPCR validation of LncRNA AK144717 and Gm4316 (ENSMUST00000212411) in all the mice of sham and TAC group ( n = 6–7). K. qPCR analysis of LncRNA AK144717 expression in the TAC models of different time ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; NS means not significant between groups

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Long noncoding RNA AK144717 exacerbates pathological cardiac hypertrophy through modulating the cellular distribution of HMGB1 and subsequent DNA damage response

doi: 10.1007/s00018-024-05464-0

Figure Lengend Snippet: Establishment of cardiac failure model and identification of altered lncRNAs. A–C . Echocardiography and quantitative analysis ( n = 6–7). D–E . Representative images of hematoxylin and eosin (H&E) staining (upper) and wheat germ agglutinin (WGA) staining ( n = 6–7). Scale bar: 100 μm. F . Quantitative analysis of the heart weight (HW)/body weight (BW) ratio ( n = 6–7). G . qPCR analysis of ANP and BNP mRNA expressions ( n = 6–7). H . Heat map of the top 20 altered lncRNAs in the heart. The lncRNAs with raw intensities over 20 in the microarray sequencing report are framed in red. I . qPCR analysis of lncRNAs in the samples applied for microarray sequencing ( n = 3). J . qPCR validation of LncRNA AK144717 and Gm4316 (ENSMUST00000212411) in all the mice of sham and TAC group ( n = 6–7). K. qPCR analysis of LncRNA AK144717 expression in the TAC models of different time ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; NS means not significant between groups

Article Snippet: The ventricular samples from 3 mice operated by TAC for 6 weeks and 3 mice with sham surgery were sent to Shanghai KangChen Bio-tech Company to detect the differentially expressed lncRNAs by using Arraystar mouse lncRNA microarray V4.

Techniques: Staining, Microarray, Sequencing, Biomarker Discovery, Expressing