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goat polyclonal antibody  (R&D Systems)


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    Structured Review

    R&D Systems goat polyclonal antibody

    Goat Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+ip/Mouse+CXCL10%2FIP-10%2FCRG-2+Antibody/pmc07375817-9-9-4
    Average 93 stars, based on 63 article reviews
    goat polyclonal antibody - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "A new genetic strategy for targeting microglia in development and disease"

    Article Title: A new genetic strategy for targeting microglia in development and disease

    Journal: eLife

    doi: 10.7554/eLife.54590


    Figure Legend Snippet:

    Techniques Used:

    Related Articles

    Injection:

    Article Title: IL-25-ILC2-IL-13 axis improves traumatic brain injury by mediating CXCL-10-dependent regulation of blood brain barrier integrity.
    Article Snippet: Clone 35B (514418, BioLegend, USA) was administered via i.p. injection at a dose of 100 μg per mouse 1 day before TBI, followed by daily injections for two consecutive days [30]. .. An anti-mouse CXCL-10 neutralizing antibody (AF-466-NA, R&D SYSTEMS, USA) was administered via i.p. injection at a dose of 50 μg per mouse 1 day before TBI, followed by daily injections for two consecutive days [31]. .. An anti-mouse IL-13 neutralizing antibody (MAB413, R&D SYSTEMS, USA) was administered via i.p. injection at a dose of 100 μg per mouse 1 day before TBI, followed by daily injections for two consecutive days [32].

    Incubation:

    Article Title: Endothelial IRE1 signaling maintains blood-brain barrier integrity and limits neuroinflammation after traumatic brain injury.
    Article Snippet: The brains were then cryoprotected in 30% sucrose in phosphate buffer at 4 °C for 2 days and were coronally sectioned at 30 μm thickness using a cryostat (Leica Biosystems, Wetzlar, Germany). .. Sections were blocked with 3% bovine serum albumin in 0.3% Triton X-100 for 1 h at room temperature and were incubated overnight at 4 °C with the following primary antibodies: anti-GFP (598, MBL, Tokyo, Japan; RRID:AB_591819, 1:1000), anti-CD31 (550274, BD Pharmingen, Franklin Lakes, NJ; RRID:AB_393571, 1:100), anti-VE-cadherin (AF1002, R&D Systems, Abingdon, UK; RRID:AB_2077789, 1:100), antiCXCL10 (AF-466-NA, R&D Systems; RRID:AB_2292487, 1:200), anti-CD45 (550539, BD Pharmingen; RRID:AB_2174426, 1:200), anti-Laminin (L9393, Sigma, St. Louis, MO; RRID:AB_477163, 1:4000), anti-NeuN (24307, Cell Signaling Technology, Danvers, MA, USA; RRID:AB_2651140), and anti-Iba1 (ab107159, Abcam, Cambridge, UK; RRID:AB_10972670, 1:1000; and 019- 19741, Wako; RRID:AB_839504, 1:500). anti-ERG (ab92513, Abcam, Cambridge, UK; RRID:AB_2630401, 1:800), anti-SMI31 (NE1022, Millipore; RRID:AB_2043448, 1:800), anti-IRE1α (NB100-2323, Novus; RRID: AB_10145203, 1:500), anti-p62 (018-22141, Wako; RRID:AB_10658438, 1:500), Secondary antibodies conjugated to Alexa Fluor 488 or Cy3 were used for fluorescence detection. ..

    Article Title: IL-25–ILC2–IL-13 axis improves traumatic brain injury by mediating CXCL-10-dependent regulation of blood brain barrier integrity
    Article Snippet: Proteins were denatured at 95 °C for 10 min, separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE; P0015L, Beyotime, China), and transferred onto polyvinylidene difluoride membranes (IPVH00010, Millipore, USA). .. Membranes were blocked with 5% nonfat dried milk for 2 h at room temperature, followed by overnight incubation at 4 °C with the following primary antibodies: rabbit anti- zonula occludens-1 (anti-ZO-1, 1:1000, 13663, Cell Signaling Technology, USA), rabbit anti-occludin (1:1000, ab216327, Abcam, UK), rabbit anti-claudin-5 (1:2000, A10207 , ABclonal, China), rabbit anti-caspase-1 (1:1000, ab207802, Abcam, UK), goat anti-CXCL-10 (1 μg/mL, AF-466-NA, R&D Systems, USA), rabbit anti-β-Actin (1:10,000, 66009-1-Ig, Proteintech, China), and rabbit anti-GAPDH (1:10,000, 10494-1-AP, Proteintech, China). .. Membranes were washed three times with Tris-buffered saline containing 0.1% Tween-20 for 10 min each, then incubated for 1 h at room temperature with species-matched horseradish peroxidase-conjugated secondary antibodies: anti-rabbit immunoglobulin G (IgG, 1:10,000, Abs20040, Absin Bioscience, China) and anti-goat IgG (1:10,000, A21030, Abbkine Scientific, China).

    Article Title: IL-25-ILC2-IL-13 axis improves traumatic brain injury by mediating CXCL-10-dependent regulation of blood brain barrier integrity.
    Article Snippet: Proteins were denatured at 95 °C for 10 min, separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE; P0015L, Beyotime, China), and transferred onto polyvinylidene difluoride membranes (IPVH00010, Millipore, USA). .. Membranes were blocked with 5% nonfat dried milk for 2 h at room temperature, followed by overnight incubation at 4 °C with the following primary antibodies: rabbit anti- zonula occludens-1 (anti-ZO-1, 1:1000, 13663, Cell Signaling Technology, USA), rabbit anti-occludin (1:1000, ab216327, Abcam, UK), rabbit anti-claudin-5 (1:2000, A10207, ABclonal, China), rabbit anti-caspase-1 (1:1000, ab207802, Abcam, UK), goat anti-CXCL-10 (1 μg/mL, AF-466-NA, R&D Systems, USA), rabbit anti-β-Actin (1:10,000, 66009-1-Ig, Proteintech, China), and rabbit anti-GAPDH (1:10,000, 10494-1-AP, Proteintech, China). .. Membranes were washed three times with Tris-buffered saline containing 0.1% Tween-20 for 10 min each, then incubated for 1 h at room temperature with species-matched horseradish peroxidase-conjugated secondary antibodies: anti-rabbit immunoglobulin G (IgG, 1:10,000, Abs20040, Absin Bioscience, China) and anti-goat IgG (1:10,000, A21030, Abbkine Scientific, China).

    Article Title: Endothelial IRE1 signaling maintains blood–brain barrier integrity and limits neuroinflammation after traumatic brain injury
    Article Snippet: The brains were then cryoprotected in 30% sucrose in phosphate buffer at 4 °C for 2 days and were coronally sectioned at 30 μm thickness using a cryostat (Leica Biosystems, Wetzlar, Germany). .. Sections were blocked with 3% bovine serum albumin in 0.3% Triton X-100 for 1 h at room temperature and were incubated overnight at 4 °C with the following primary antibodies: anti-GFP (598, MBL, Tokyo, Japan; RRID:AB_591819, 1:1000), anti-CD31 (550274, BD Pharmingen, Franklin Lakes, NJ; RRID:AB_393571, 1:100), anti-VE-cadherin (AF1002, R&D Systems, Abingdon, UK; RRID:AB_2077789, 1:100), anti-CXCL10 (AF-466-NA, R&D Systems; RRID:AB_2292487, 1:200), anti-CD45 (550539, BD Pharmingen; RRID:AB_2174426, 1:200), anti-Laminin (L9393, Sigma, St. Louis, MO; RRID:AB_477163, 1:4000), anti-NeuN (24307, Cell Signaling Technology, Danvers, MA, USA; RRID:AB_2651140), and anti-Iba1 (ab107159, Abcam, Cambridge, UK; RRID:AB_10972670, 1:1000; and 019-19741, Wako; RRID:AB_839504, 1:500). anti-ERG (ab92513, Abcam, Cambridge, UK; RRID:AB_2630401, 1:800), anti-SMI31 (NE1022, Millipore; RRID:AB_2043448, 1:800), anti-IRE1α (NB100-2323, Novus; RRID: AB_10145203, 1:500), anti-p62 (018-22141, Wako; RRID:AB_10658438, 1:500), Secondary antibodies conjugated to Alexa Fluor 488 or Cy3 were used for fluorescence detection. ..

    Fluorescence:

    Article Title: Endothelial IRE1 signaling maintains blood-brain barrier integrity and limits neuroinflammation after traumatic brain injury.
    Article Snippet: The brains were then cryoprotected in 30% sucrose in phosphate buffer at 4 °C for 2 days and were coronally sectioned at 30 μm thickness using a cryostat (Leica Biosystems, Wetzlar, Germany). .. Sections were blocked with 3% bovine serum albumin in 0.3% Triton X-100 for 1 h at room temperature and were incubated overnight at 4 °C with the following primary antibodies: anti-GFP (598, MBL, Tokyo, Japan; RRID:AB_591819, 1:1000), anti-CD31 (550274, BD Pharmingen, Franklin Lakes, NJ; RRID:AB_393571, 1:100), anti-VE-cadherin (AF1002, R&D Systems, Abingdon, UK; RRID:AB_2077789, 1:100), antiCXCL10 (AF-466-NA, R&D Systems; RRID:AB_2292487, 1:200), anti-CD45 (550539, BD Pharmingen; RRID:AB_2174426, 1:200), anti-Laminin (L9393, Sigma, St. Louis, MO; RRID:AB_477163, 1:4000), anti-NeuN (24307, Cell Signaling Technology, Danvers, MA, USA; RRID:AB_2651140), and anti-Iba1 (ab107159, Abcam, Cambridge, UK; RRID:AB_10972670, 1:1000; and 019- 19741, Wako; RRID:AB_839504, 1:500). anti-ERG (ab92513, Abcam, Cambridge, UK; RRID:AB_2630401, 1:800), anti-SMI31 (NE1022, Millipore; RRID:AB_2043448, 1:800), anti-IRE1α (NB100-2323, Novus; RRID: AB_10145203, 1:500), anti-p62 (018-22141, Wako; RRID:AB_10658438, 1:500), Secondary antibodies conjugated to Alexa Fluor 488 or Cy3 were used for fluorescence detection. ..

    Article Title: Endothelial IRE1 signaling maintains blood–brain barrier integrity and limits neuroinflammation after traumatic brain injury
    Article Snippet: The brains were then cryoprotected in 30% sucrose in phosphate buffer at 4 °C for 2 days and were coronally sectioned at 30 μm thickness using a cryostat (Leica Biosystems, Wetzlar, Germany). .. Sections were blocked with 3% bovine serum albumin in 0.3% Triton X-100 for 1 h at room temperature and were incubated overnight at 4 °C with the following primary antibodies: anti-GFP (598, MBL, Tokyo, Japan; RRID:AB_591819, 1:1000), anti-CD31 (550274, BD Pharmingen, Franklin Lakes, NJ; RRID:AB_393571, 1:100), anti-VE-cadherin (AF1002, R&D Systems, Abingdon, UK; RRID:AB_2077789, 1:100), anti-CXCL10 (AF-466-NA, R&D Systems; RRID:AB_2292487, 1:200), anti-CD45 (550539, BD Pharmingen; RRID:AB_2174426, 1:200), anti-Laminin (L9393, Sigma, St. Louis, MO; RRID:AB_477163, 1:4000), anti-NeuN (24307, Cell Signaling Technology, Danvers, MA, USA; RRID:AB_2651140), and anti-Iba1 (ab107159, Abcam, Cambridge, UK; RRID:AB_10972670, 1:1000; and 019-19741, Wako; RRID:AB_839504, 1:500). anti-ERG (ab92513, Abcam, Cambridge, UK; RRID:AB_2630401, 1:800), anti-SMI31 (NE1022, Millipore; RRID:AB_2043448, 1:800), anti-IRE1α (NB100-2323, Novus; RRID: AB_10145203, 1:500), anti-p62 (018-22141, Wako; RRID:AB_10658438, 1:500), Secondary antibodies conjugated to Alexa Fluor 488 or Cy3 were used for fluorescence detection. ..

    Staining:

    Article Title: Endotoxin Tolerance Impinges on T Cell Activation and Chemoattraction in Autoimmune Diabetes
    Article Snippet: Blocking was done with 5% BSA (Sigma–Aldrich, #A9418‐50G) in PBS. .. The sections were stained with primary antibody anti m‐CXCL10/IP‐10 goat IgG (R&D Systems Inc., #AF‐466‐NA) for 50 min and with secondary antibody Alexa Fluor 555 donkey anti‐goat IgG (H + L; Invitrogen, #A‐21432) for 30 min. .. Sections were mounted with ProLong Diamond Antifade Mountant (Invitrogen, # P36965 ) and coverglass.

    Recombinant:

    Article Title: Macrophage TRIM21 lactylation exacerbates infection-induced orchitis through enhancing STAT1-mediated CXCL9 and CXCL10 production
    Article Snippet: .. The recombinant neutralizing antibodies anti-CXCL9 (AF-492-NA, R&D Systems, Minneapolis, MN, USA) and anti-CXCL10 (AF-466-NA, R&D Systems, Minneapolis, MN, USA) were diluted in sterile PBS and stored at −20°C. ..

    Sterility:

    Article Title: Macrophage TRIM21 lactylation exacerbates infection-induced orchitis through enhancing STAT1-mediated CXCL9 and CXCL10 production
    Article Snippet: .. The recombinant neutralizing antibodies anti-CXCL9 (AF-492-NA, R&D Systems, Minneapolis, MN, USA) and anti-CXCL10 (AF-466-NA, R&D Systems, Minneapolis, MN, USA) were diluted in sterile PBS and stored at −20°C. ..



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    CXCL10 is upregulated in both macrophages and fibroblasts responding to aged ZnO NPs exposure. (A) Heatmaps exhibited multiplex cytokine profiling of serum and BALF from IT (PBS/fresh NPs/aged NPs intratracheal instillation) and IT + IV (IT exposure followed by intravenous injection of 4T1 cells) models ( n = 3). (B–E) Concentrations of significantly dyregulated cytokines in serum and BALF from mice exposed to ZnO NPs (fresh or aged), with or without subsequent 4T1 cell injection ( n = 3). (F) Venn plot showed the overlapping cytokines in serum and BALF between IT and IT + IV models. (G) ELISA detection of CXCL10 concentrations in serum and BALF of IT and IT + IV groups ( n = 3). (H) qPCR analysis of CXCL10 mRNA in lung tissue, primary fibroblasts, and alveolar macrophages post‐IT, normalized to GAPDH ( n = 3). (I, J) UMAP visualization (I) and dot plot (J) displaying CXCL10 expression across annotated cell types. (K, L) UMAP visualization (K) and dot plot (L) displaying CXCL10 expression across myeloid cell types. (M) Representative images of invasion and migration assays of 4T1 cells stimulated with 50 or 100 ng/mL murine recombinant CXCL10 (rCXCL10) for 72 h ( n = 3). (N) Quantification of invasion and migration assays ( n = 3). All data were represented as the mean ± SD. P values were calculated by one‐way ANOVA followed by Tukey's test. * p < 0.05, ** p < 0.01 vs control group; # p < 0.05, ## p < 0.01 vs fresh NPs group.

    Journal: Advanced Science

    Article Title: Aged ZnO Nanoparticles Promote Breast Cancer Lung Metastasis by Remodeling the Pulmonary Microenvironment Through the CXCL10/Wnt/β‐Catenin Axis

    doi: 10.1002/advs.78249

    Figure Lengend Snippet: CXCL10 is upregulated in both macrophages and fibroblasts responding to aged ZnO NPs exposure. (A) Heatmaps exhibited multiplex cytokine profiling of serum and BALF from IT (PBS/fresh NPs/aged NPs intratracheal instillation) and IT + IV (IT exposure followed by intravenous injection of 4T1 cells) models ( n = 3). (B–E) Concentrations of significantly dyregulated cytokines in serum and BALF from mice exposed to ZnO NPs (fresh or aged), with or without subsequent 4T1 cell injection ( n = 3). (F) Venn plot showed the overlapping cytokines in serum and BALF between IT and IT + IV models. (G) ELISA detection of CXCL10 concentrations in serum and BALF of IT and IT + IV groups ( n = 3). (H) qPCR analysis of CXCL10 mRNA in lung tissue, primary fibroblasts, and alveolar macrophages post‐IT, normalized to GAPDH ( n = 3). (I, J) UMAP visualization (I) and dot plot (J) displaying CXCL10 expression across annotated cell types. (K, L) UMAP visualization (K) and dot plot (L) displaying CXCL10 expression across myeloid cell types. (M) Representative images of invasion and migration assays of 4T1 cells stimulated with 50 or 100 ng/mL murine recombinant CXCL10 (rCXCL10) for 72 h ( n = 3). (N) Quantification of invasion and migration assays ( n = 3). All data were represented as the mean ± SD. P values were calculated by one‐way ANOVA followed by Tukey's test. * p < 0.05, ** p < 0.01 vs control group; # p < 0.05, ## p < 0.01 vs fresh NPs group.

    Article Snippet: CXCL10 (IP‐10) levels were measured using a mouse ELISA kit (Elabscience, E‐EL‐M0021) following the manufacturer's instructions.

    Techniques: Multiplex Assay, Profiling, Injection, Enzyme-linked Immunosorbent Assay, Analysis, Tissue, Expressing, Migration, Recombinant, Control