mouse ige quantification kit  (Fortis Life Sciences)

Journal: Microbial Biotechnology

Article Title: Polysaccharides L900/2 and L900/3 isolated from Lactobacillus rhamnosus LOCK 0900 modulate allergic sensitization to ovalbumin in a mouse model

doi: 10.1111/1751-7915.12606

Figure Lengend Snippet: Levels of OVA ‐specific IgE (A), IgG2a (B), IgG1 (C) and IgA (D) antibodies in serum, as measured by ELISA . Pooled values of two repeat experiments, each with four to six mice per group, are expressed as means ± SEM ; * P < 0.05, *** P < 0.001, **** P < 0.0001.

Article Snippet: The serum levels of total IgA and IgE were measured using a mouse IgA and IgE ELISA quantification kit (Bethyl, Montgomery, TX, USA) according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Microbial Biotechnology

Article Title: Polysaccharides L900/2 and L900/3 isolated from Lactobacillus rhamnosus LOCK 0900 modulate allergic sensitization to ovalbumin in a mouse model

doi: 10.1111/1751-7915.12606

Figure Lengend Snippet: Levels of total IgE (A) and IgA (B) in serum, as measured by ELISA . Pooled values of two repeat experiments, each with four to six mice per group.

Article Snippet: The serum levels of total IgA and IgE were measured using a mouse IgA and IgE ELISA quantification kit (Bethyl, Montgomery, TX, USA) according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Cellular & molecular immunology

Article Title: Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization.

doi: 10.1038/cmi.2015.09

Figure Lengend Snippet: Figure 4 Sensitization of GF and Lmix-colonized mice with the major birch pollen allergen Bet v 1. (a) The experimental design was as follows: 8-week- old GF mice (n512) were divided into two groups. The first group (Lmix) received an equal-part mixture (23108 CFU/ml) of L. rhamnosus LOCK0900, L. rhamnosus LOCK0908 and L. casei LOCK0919 by intragastric tubing. The second group was kept GF. Mice were sensitized three times intra- peritoneally with recombinant Bet v 1 (1 mg in alum) on days 77, 87 and 97. One week after the last immunization (day 104), tissue samples were collected for further analyses. Bacterial colonization of the Lmix-colonized mice was evaluated on the first 2 days and then at weekly intervals throughout the experiment. At the species level, bacteria were distinguished based on colony morphology by the cultivation of appropriate serial dilutions of the fecal samples. (b) L. casei LOCK0919 (full circles, solid line), L. rhamnosus LOCK0900 and LOCK0908 (open squares, dotted line). L. rhamnosus strain- specific discrimination was performed by qPCR using DNA isolated from the fecal samples at the indicated time points (c) L. rhamnosus LOCK0900 (gray bars) and L. rhamnosus LOCK0908 (black bars). The data are shown as a percentage of each strain out of all detected L. rhamnosus bacteria on the indicated day after colonization. Bet v 1-specific antibodies were measured in the sera of GF (white bars) and Lmix-colonized mice (black bars). IgE was measured by Bet v 1-mediated b-hexosaminidase release from rat basophil leukemia cells (d). Levels of IgG1 (e) and IgG2a (f) were evaluated by ELISA and expressed as OD units. The data are shown as the mean6s.e.m. One representative out of two experiments is shown; n56/group. *P,0.05 and **P,0.01. GF, germ-free; Ig, immunoglobulin; i.p., intraperitoneally; Lmix, Lactobacillus mixture; OD, optical density.

Article Snippet: Total IgA and IgE responses Total IgA and IgE were measured in the sera and gut lavages (IgA only) with a mouse IgA and IgE ELISA quantification kit (Bethyl, Montgomery, TX, USA) according to manufacturer’s instructions.

Techniques: Recombinant, Bacteria, Isolation, Enzyme-linked Immunosorbent Assay

Journal: Cellular & molecular immunology

Article Title: Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization.

doi: 10.1038/cmi.2015.09

Figure Lengend Snippet: Figure 5 Local and systemic humoral responses in sensitized GF and Lmix-colonized mice. Levels of total IgE (a) and total IgA (b) in the sera and total IgA (c) in the gut lavage were measured by ELISA. Germ-free mice (GF, white bars) and Lmix-colonized mice (Lmix, black bars). The data are shown as the mean6s.e.m. One representative out of two experiments is shown; n56/group. *P,0.05 and **P,0.01. IgA-positive plasmocytes in the lamina propria of the terminal ileum were visualized with an FITC-labeled anti-IgA antibody (d). GF, germ-free; Ig, immunoglobulin; Lmix, Lactobacillus mixture.

Article Snippet: Total IgA and IgE responses Total IgA and IgE were measured in the sera and gut lavages (IgA only) with a mouse IgA and IgE ELISA quantification kit (Bethyl, Montgomery, TX, USA) according to manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Labeling