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    FUJIFILM hydroxypropyl methylcellulose fujifilm
    Hydroxypropyl Methylcellulose Fujifilm, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+gem+2%2E08+cdna+microarray/hydroxypropyl+methylcellulose+fujifilm/pm33626352-666-2-7
    Average 90 stars, based on 1 article reviews
    hydroxypropyl methylcellulose fujifilm - by Bioz Stars, 2026-10
    90/100 stars

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    Recombinant:

    Article Title: Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Monoclonal Anti-Human Cytokeratin (clones AE1/AE3) DAKO Cat# M3515 LOT: 10066159 Rabbit Polyclonal Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Cell Signaling Technology Cat# 4370 RRID:AB_2315112 Rabbit polyclonal Anti-alpha smooth muscle Actin [1A4] Abcam Cat# ab7817 Rabbit polyclonal Anti-S100 antibody Abcam Cat# ab166649 Rabbit polyclonal Anti-FGF10 antibody (for mouse) Santa Cruz Cat# sc-293208 Rabbit polyclonal Anti-FGF10 antibody (for human) Abcam Cat# ab80064 Rabbit polyclonal Anti-FGF10 antibody Sigma-Aldrich Cat#ABN44 Mouse monoclonal Anti-MUC5AC antibody Abcam Cat# ab3649 Mouse monoclonal Anti-MUC6 antibody Leica Biosystems Cat# 130307 Rabbit polyclonal Anti-MUC2 antibody Novus Biologicals Cat# NBP1-31231 Rabbit monoclonal Anti-MUC1 antibody Invitrogen Cat# MA5-35250 Rabbit polyclonal Anti-Phospho-FGFR Pan (Tyr653, Tyr654) antibody Invitrogen Cat# PA5-105938 Rabbit polyclonal Anti-Src (phospho Y418) antibody Abcam Cat# ab4816 Rabbit Polyclonal RFP Antibody Preadsorbed Rockland Immunochemicals Cat# 600-401-379 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed Abcam Cat# ab150117 Goat Anti-Rabbit IgG H&L (Alexa Fluor 594) preadsorbed Abcam Cat# ab150084 Rabbit IgG (Control Antibody) Vector Laboratories Cat# I-1000 Mouse IgG (Control Antibody) Vector Laboratories Cat# I-2000 Bacterial and virus strains Escherichia coli strain SW105 Frederick National Laboratory Cancer Research, NCI NA Biological samples Human biliary tumor samples This paper NA Chemicals, peptides, and recombinant proteins Tamoxifen Sigma Cat# T5648 Recommbinant mouse FGF10 R&D Systems Cat# 6224-FG Recommbinant human FGF10 R&D Systems Cat# 345-FG-025 thioacetamide Sigma Cat# 163678 Doxycycline hyclate Sigma Cat# D9891 Polyinosinic-Polycytidylic acid Sigma Cat# P1530 - Cellstain - DAPI solution DOJINDO Cat# D523 PD0325901 Selleck Chemicals Cat# S1036 L-arabinose Sigma Cat# A3256 Sucrose FUJIFILM Cat# 192-00017 dimethyl sulfoxide FUJIFILM Cat# 043-07211 (Continued on next page) Cell Reports 34, 108772, February 23, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydroxypropyl methylcellulose FUJIFILM Cat# 515-70211 Polyoxyethylene(20) Sorbitan Monolaurate FUJIFILM Cat# 165-11511 MEM with non-essential amino acids Thermo Fisher Scientific Cat# 11140-019, used in BEC media Insulin-transferrin-selenium Thermo Fisher Scientific Cat# 51300-044, used in BEC media Na Pyruvate Thermo Fisher Scientific Cat# 11360070, used in BEC media Chemically-defined lipid concentrate Thermo Fisher Scientific Cat# 11905-031, used in BEC media Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122, used in BEC media Gentamicin Thermo Fisher Scientific Cat# 15750-060, used in BEC media Ethanolamine Sigma Cat# E9508, used in BEC media MEM vitamin solution Thermo Fisher Scientific Cat# 11120-052, used in BEC media Soybean trypsin inhibitor FUJIFILM Cat# 208-09223, used in BEC media L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030149, used in BEC media Bovine pituitary extract Thermo Fisher Scientific Cat# 13028014, used in BEC media Dexamethasone Sigma Cat# D4902, used in BEC media 3 30,5-triiodo-L-thyronine Sigma Cat# 01-101, used in BEC media Epidermal growth factor Merck, Millipore Cat# 01-101, used in BEC media Amphotericin B Sigma Cat# F6886, used in BEC media 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 NVP-BGJ398 Chemscene Cat# CS-0586 ESGRO Recombinant Mouse LIF Protein Merck, Millipore Cat# ESG1107 Critical commercial assays Vectastain Universal Elite ABC kit (mouse IgG) Vector Laboratories Cat# PK-6102 Vectastain Universal Elite ABC kit (Rabbit IgG) Vector Laboratories Cat# PK-6101 SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat# 18080051 Rneasy Mini Kit QIAGEN Cat# 74104 RNAscope 2.5 HD Reagent Kit- BROWN ACD Cat# 322310 iPGell Genostaff Cat# PG20-1 Deposited data RNA microarray raw data This paper GEO: GSE 119227 Experimental Models: Cell Lines KH2 embryonic stem cells Beard et al., 2006 NA KBDC-11 cells (IPNB cells) Murakami et al., 2014 NA KKU-213 cells (papillary cholangiocarcinoma) JCRB cell bank Cat# JCRB1557 PA6 cells Riken Bioresource Center Cell Bank Cat# RCB1127 Experimental models: organisms/strains C57BL/6J The Jackson Laboratory Stock# 00064 CAnN.Cg-Foxn1nu/CrlCrlj (BALB/c-nu) Charles River Laboratories NA Krt19-iCre (BAC) Kanayama et al., 2019 Generated. ..

    RNAscope:

    Article Title: Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Monoclonal Anti-Human Cytokeratin (clones AE1/AE3) DAKO Cat# M3515 LOT: 10066159 Rabbit Polyclonal Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Cell Signaling Technology Cat# 4370 RRID:AB_2315112 Rabbit polyclonal Anti-alpha smooth muscle Actin [1A4] Abcam Cat# ab7817 Rabbit polyclonal Anti-S100 antibody Abcam Cat# ab166649 Rabbit polyclonal Anti-FGF10 antibody (for mouse) Santa Cruz Cat# sc-293208 Rabbit polyclonal Anti-FGF10 antibody (for human) Abcam Cat# ab80064 Rabbit polyclonal Anti-FGF10 antibody Sigma-Aldrich Cat#ABN44 Mouse monoclonal Anti-MUC5AC antibody Abcam Cat# ab3649 Mouse monoclonal Anti-MUC6 antibody Leica Biosystems Cat# 130307 Rabbit polyclonal Anti-MUC2 antibody Novus Biologicals Cat# NBP1-31231 Rabbit monoclonal Anti-MUC1 antibody Invitrogen Cat# MA5-35250 Rabbit polyclonal Anti-Phospho-FGFR Pan (Tyr653, Tyr654) antibody Invitrogen Cat# PA5-105938 Rabbit polyclonal Anti-Src (phospho Y418) antibody Abcam Cat# ab4816 Rabbit Polyclonal RFP Antibody Preadsorbed Rockland Immunochemicals Cat# 600-401-379 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed Abcam Cat# ab150117 Goat Anti-Rabbit IgG H&L (Alexa Fluor 594) preadsorbed Abcam Cat# ab150084 Rabbit IgG (Control Antibody) Vector Laboratories Cat# I-1000 Mouse IgG (Control Antibody) Vector Laboratories Cat# I-2000 Bacterial and virus strains Escherichia coli strain SW105 Frederick National Laboratory Cancer Research, NCI NA Biological samples Human biliary tumor samples This paper NA Chemicals, peptides, and recombinant proteins Tamoxifen Sigma Cat# T5648 Recommbinant mouse FGF10 R&D Systems Cat# 6224-FG Recommbinant human FGF10 R&D Systems Cat# 345-FG-025 thioacetamide Sigma Cat# 163678 Doxycycline hyclate Sigma Cat# D9891 Polyinosinic-Polycytidylic acid Sigma Cat# P1530 - Cellstain - DAPI solution DOJINDO Cat# D523 PD0325901 Selleck Chemicals Cat# S1036 L-arabinose Sigma Cat# A3256 Sucrose FUJIFILM Cat# 192-00017 dimethyl sulfoxide FUJIFILM Cat# 043-07211 (Continued on next page) Cell Reports 34, 108772, February 23, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydroxypropyl methylcellulose FUJIFILM Cat# 515-70211 Polyoxyethylene(20) Sorbitan Monolaurate FUJIFILM Cat# 165-11511 MEM with non-essential amino acids Thermo Fisher Scientific Cat# 11140-019, used in BEC media Insulin-transferrin-selenium Thermo Fisher Scientific Cat# 51300-044, used in BEC media Na Pyruvate Thermo Fisher Scientific Cat# 11360070, used in BEC media Chemically-defined lipid concentrate Thermo Fisher Scientific Cat# 11905-031, used in BEC media Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122, used in BEC media Gentamicin Thermo Fisher Scientific Cat# 15750-060, used in BEC media Ethanolamine Sigma Cat# E9508, used in BEC media MEM vitamin solution Thermo Fisher Scientific Cat# 11120-052, used in BEC media Soybean trypsin inhibitor FUJIFILM Cat# 208-09223, used in BEC media L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030149, used in BEC media Bovine pituitary extract Thermo Fisher Scientific Cat# 13028014, used in BEC media Dexamethasone Sigma Cat# D4902, used in BEC media 3 30,5-triiodo-L-thyronine Sigma Cat# 01-101, used in BEC media Epidermal growth factor Merck, Millipore Cat# 01-101, used in BEC media Amphotericin B Sigma Cat# F6886, used in BEC media 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 NVP-BGJ398 Chemscene Cat# CS-0586 ESGRO Recombinant Mouse LIF Protein Merck, Millipore Cat# ESG1107 Critical commercial assays Vectastain Universal Elite ABC kit (mouse IgG) Vector Laboratories Cat# PK-6102 Vectastain Universal Elite ABC kit (Rabbit IgG) Vector Laboratories Cat# PK-6101 SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat# 18080051 Rneasy Mini Kit QIAGEN Cat# 74104 RNAscope 2.5 HD Reagent Kit- BROWN ACD Cat# 322310 iPGell Genostaff Cat# PG20-1 Deposited data RNA microarray raw data This paper GEO: GSE 119227 Experimental Models: Cell Lines KH2 embryonic stem cells Beard et al., 2006 NA KBDC-11 cells (IPNB cells) Murakami et al., 2014 NA KKU-213 cells (papillary cholangiocarcinoma) JCRB cell bank Cat# JCRB1557 PA6 cells Riken Bioresource Center Cell Bank Cat# RCB1127 Experimental models: organisms/strains C57BL/6J The Jackson Laboratory Stock# 00064 CAnN.Cg-Foxn1nu/CrlCrlj (BALB/c-nu) Charles River Laboratories NA Krt19-iCre (BAC) Kanayama et al., 2019 Generated. ..

    Microarray:

    Article Title: Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Monoclonal Anti-Human Cytokeratin (clones AE1/AE3) DAKO Cat# M3515 LOT: 10066159 Rabbit Polyclonal Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Cell Signaling Technology Cat# 4370 RRID:AB_2315112 Rabbit polyclonal Anti-alpha smooth muscle Actin [1A4] Abcam Cat# ab7817 Rabbit polyclonal Anti-S100 antibody Abcam Cat# ab166649 Rabbit polyclonal Anti-FGF10 antibody (for mouse) Santa Cruz Cat# sc-293208 Rabbit polyclonal Anti-FGF10 antibody (for human) Abcam Cat# ab80064 Rabbit polyclonal Anti-FGF10 antibody Sigma-Aldrich Cat#ABN44 Mouse monoclonal Anti-MUC5AC antibody Abcam Cat# ab3649 Mouse monoclonal Anti-MUC6 antibody Leica Biosystems Cat# 130307 Rabbit polyclonal Anti-MUC2 antibody Novus Biologicals Cat# NBP1-31231 Rabbit monoclonal Anti-MUC1 antibody Invitrogen Cat# MA5-35250 Rabbit polyclonal Anti-Phospho-FGFR Pan (Tyr653, Tyr654) antibody Invitrogen Cat# PA5-105938 Rabbit polyclonal Anti-Src (phospho Y418) antibody Abcam Cat# ab4816 Rabbit Polyclonal RFP Antibody Preadsorbed Rockland Immunochemicals Cat# 600-401-379 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed Abcam Cat# ab150117 Goat Anti-Rabbit IgG H&L (Alexa Fluor 594) preadsorbed Abcam Cat# ab150084 Rabbit IgG (Control Antibody) Vector Laboratories Cat# I-1000 Mouse IgG (Control Antibody) Vector Laboratories Cat# I-2000 Bacterial and virus strains Escherichia coli strain SW105 Frederick National Laboratory Cancer Research, NCI NA Biological samples Human biliary tumor samples This paper NA Chemicals, peptides, and recombinant proteins Tamoxifen Sigma Cat# T5648 Recommbinant mouse FGF10 R&D Systems Cat# 6224-FG Recommbinant human FGF10 R&D Systems Cat# 345-FG-025 thioacetamide Sigma Cat# 163678 Doxycycline hyclate Sigma Cat# D9891 Polyinosinic-Polycytidylic acid Sigma Cat# P1530 - Cellstain - DAPI solution DOJINDO Cat# D523 PD0325901 Selleck Chemicals Cat# S1036 L-arabinose Sigma Cat# A3256 Sucrose FUJIFILM Cat# 192-00017 dimethyl sulfoxide FUJIFILM Cat# 043-07211 (Continued on next page) Cell Reports 34, 108772, February 23, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydroxypropyl methylcellulose FUJIFILM Cat# 515-70211 Polyoxyethylene(20) Sorbitan Monolaurate FUJIFILM Cat# 165-11511 MEM with non-essential amino acids Thermo Fisher Scientific Cat# 11140-019, used in BEC media Insulin-transferrin-selenium Thermo Fisher Scientific Cat# 51300-044, used in BEC media Na Pyruvate Thermo Fisher Scientific Cat# 11360070, used in BEC media Chemically-defined lipid concentrate Thermo Fisher Scientific Cat# 11905-031, used in BEC media Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122, used in BEC media Gentamicin Thermo Fisher Scientific Cat# 15750-060, used in BEC media Ethanolamine Sigma Cat# E9508, used in BEC media MEM vitamin solution Thermo Fisher Scientific Cat# 11120-052, used in BEC media Soybean trypsin inhibitor FUJIFILM Cat# 208-09223, used in BEC media L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030149, used in BEC media Bovine pituitary extract Thermo Fisher Scientific Cat# 13028014, used in BEC media Dexamethasone Sigma Cat# D4902, used in BEC media 3 30,5-triiodo-L-thyronine Sigma Cat# 01-101, used in BEC media Epidermal growth factor Merck, Millipore Cat# 01-101, used in BEC media Amphotericin B Sigma Cat# F6886, used in BEC media 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 NVP-BGJ398 Chemscene Cat# CS-0586 ESGRO Recombinant Mouse LIF Protein Merck, Millipore Cat# ESG1107 Critical commercial assays Vectastain Universal Elite ABC kit (mouse IgG) Vector Laboratories Cat# PK-6102 Vectastain Universal Elite ABC kit (Rabbit IgG) Vector Laboratories Cat# PK-6101 SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat# 18080051 Rneasy Mini Kit QIAGEN Cat# 74104 RNAscope 2.5 HD Reagent Kit- BROWN ACD Cat# 322310 iPGell Genostaff Cat# PG20-1 Deposited data RNA microarray raw data This paper GEO: GSE 119227 Experimental Models: Cell Lines KH2 embryonic stem cells Beard et al., 2006 NA KBDC-11 cells (IPNB cells) Murakami et al., 2014 NA KKU-213 cells (papillary cholangiocarcinoma) JCRB cell bank Cat# JCRB1557 PA6 cells Riken Bioresource Center Cell Bank Cat# RCB1127 Experimental models: organisms/strains C57BL/6J The Jackson Laboratory Stock# 00064 CAnN.Cg-Foxn1nu/CrlCrlj (BALB/c-nu) Charles River Laboratories NA Krt19-iCre (BAC) Kanayama et al., 2019 Generated. ..

    BAC Assay:

    Article Title: Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Monoclonal Anti-Human Cytokeratin (clones AE1/AE3) DAKO Cat# M3515 LOT: 10066159 Rabbit Polyclonal Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Cell Signaling Technology Cat# 4370 RRID:AB_2315112 Rabbit polyclonal Anti-alpha smooth muscle Actin [1A4] Abcam Cat# ab7817 Rabbit polyclonal Anti-S100 antibody Abcam Cat# ab166649 Rabbit polyclonal Anti-FGF10 antibody (for mouse) Santa Cruz Cat# sc-293208 Rabbit polyclonal Anti-FGF10 antibody (for human) Abcam Cat# ab80064 Rabbit polyclonal Anti-FGF10 antibody Sigma-Aldrich Cat#ABN44 Mouse monoclonal Anti-MUC5AC antibody Abcam Cat# ab3649 Mouse monoclonal Anti-MUC6 antibody Leica Biosystems Cat# 130307 Rabbit polyclonal Anti-MUC2 antibody Novus Biologicals Cat# NBP1-31231 Rabbit monoclonal Anti-MUC1 antibody Invitrogen Cat# MA5-35250 Rabbit polyclonal Anti-Phospho-FGFR Pan (Tyr653, Tyr654) antibody Invitrogen Cat# PA5-105938 Rabbit polyclonal Anti-Src (phospho Y418) antibody Abcam Cat# ab4816 Rabbit Polyclonal RFP Antibody Preadsorbed Rockland Immunochemicals Cat# 600-401-379 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed Abcam Cat# ab150117 Goat Anti-Rabbit IgG H&L (Alexa Fluor 594) preadsorbed Abcam Cat# ab150084 Rabbit IgG (Control Antibody) Vector Laboratories Cat# I-1000 Mouse IgG (Control Antibody) Vector Laboratories Cat# I-2000 Bacterial and virus strains Escherichia coli strain SW105 Frederick National Laboratory Cancer Research, NCI NA Biological samples Human biliary tumor samples This paper NA Chemicals, peptides, and recombinant proteins Tamoxifen Sigma Cat# T5648 Recommbinant mouse FGF10 R&D Systems Cat# 6224-FG Recommbinant human FGF10 R&D Systems Cat# 345-FG-025 thioacetamide Sigma Cat# 163678 Doxycycline hyclate Sigma Cat# D9891 Polyinosinic-Polycytidylic acid Sigma Cat# P1530 - Cellstain - DAPI solution DOJINDO Cat# D523 PD0325901 Selleck Chemicals Cat# S1036 L-arabinose Sigma Cat# A3256 Sucrose FUJIFILM Cat# 192-00017 dimethyl sulfoxide FUJIFILM Cat# 043-07211 (Continued on next page) Cell Reports 34, 108772, February 23, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydroxypropyl methylcellulose FUJIFILM Cat# 515-70211 Polyoxyethylene(20) Sorbitan Monolaurate FUJIFILM Cat# 165-11511 MEM with non-essential amino acids Thermo Fisher Scientific Cat# 11140-019, used in BEC media Insulin-transferrin-selenium Thermo Fisher Scientific Cat# 51300-044, used in BEC media Na Pyruvate Thermo Fisher Scientific Cat# 11360070, used in BEC media Chemically-defined lipid concentrate Thermo Fisher Scientific Cat# 11905-031, used in BEC media Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122, used in BEC media Gentamicin Thermo Fisher Scientific Cat# 15750-060, used in BEC media Ethanolamine Sigma Cat# E9508, used in BEC media MEM vitamin solution Thermo Fisher Scientific Cat# 11120-052, used in BEC media Soybean trypsin inhibitor FUJIFILM Cat# 208-09223, used in BEC media L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030149, used in BEC media Bovine pituitary extract Thermo Fisher Scientific Cat# 13028014, used in BEC media Dexamethasone Sigma Cat# D4902, used in BEC media 3 30,5-triiodo-L-thyronine Sigma Cat# 01-101, used in BEC media Epidermal growth factor Merck, Millipore Cat# 01-101, used in BEC media Amphotericin B Sigma Cat# F6886, used in BEC media 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 NVP-BGJ398 Chemscene Cat# CS-0586 ESGRO Recombinant Mouse LIF Protein Merck, Millipore Cat# ESG1107 Critical commercial assays Vectastain Universal Elite ABC kit (mouse IgG) Vector Laboratories Cat# PK-6102 Vectastain Universal Elite ABC kit (Rabbit IgG) Vector Laboratories Cat# PK-6101 SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat# 18080051 Rneasy Mini Kit QIAGEN Cat# 74104 RNAscope 2.5 HD Reagent Kit- BROWN ACD Cat# 322310 iPGell Genostaff Cat# PG20-1 Deposited data RNA microarray raw data This paper GEO: GSE 119227 Experimental Models: Cell Lines KH2 embryonic stem cells Beard et al., 2006 NA KBDC-11 cells (IPNB cells) Murakami et al., 2014 NA KKU-213 cells (papillary cholangiocarcinoma) JCRB cell bank Cat# JCRB1557 PA6 cells Riken Bioresource Center Cell Bank Cat# RCB1127 Experimental models: organisms/strains C57BL/6J The Jackson Laboratory Stock# 00064 CAnN.Cg-Foxn1nu/CrlCrlj (BALB/c-nu) Charles River Laboratories NA Krt19-iCre (BAC) Kanayama et al., 2019 Generated. ..

    Generated:

    Article Title: Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Monoclonal Anti-Human Cytokeratin (clones AE1/AE3) DAKO Cat# M3515 LOT: 10066159 Rabbit Polyclonal Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Cell Signaling Technology Cat# 4370 RRID:AB_2315112 Rabbit polyclonal Anti-alpha smooth muscle Actin [1A4] Abcam Cat# ab7817 Rabbit polyclonal Anti-S100 antibody Abcam Cat# ab166649 Rabbit polyclonal Anti-FGF10 antibody (for mouse) Santa Cruz Cat# sc-293208 Rabbit polyclonal Anti-FGF10 antibody (for human) Abcam Cat# ab80064 Rabbit polyclonal Anti-FGF10 antibody Sigma-Aldrich Cat#ABN44 Mouse monoclonal Anti-MUC5AC antibody Abcam Cat# ab3649 Mouse monoclonal Anti-MUC6 antibody Leica Biosystems Cat# 130307 Rabbit polyclonal Anti-MUC2 antibody Novus Biologicals Cat# NBP1-31231 Rabbit monoclonal Anti-MUC1 antibody Invitrogen Cat# MA5-35250 Rabbit polyclonal Anti-Phospho-FGFR Pan (Tyr653, Tyr654) antibody Invitrogen Cat# PA5-105938 Rabbit polyclonal Anti-Src (phospho Y418) antibody Abcam Cat# ab4816 Rabbit Polyclonal RFP Antibody Preadsorbed Rockland Immunochemicals Cat# 600-401-379 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed Abcam Cat# ab150117 Goat Anti-Rabbit IgG H&L (Alexa Fluor 594) preadsorbed Abcam Cat# ab150084 Rabbit IgG (Control Antibody) Vector Laboratories Cat# I-1000 Mouse IgG (Control Antibody) Vector Laboratories Cat# I-2000 Bacterial and virus strains Escherichia coli strain SW105 Frederick National Laboratory Cancer Research, NCI NA Biological samples Human biliary tumor samples This paper NA Chemicals, peptides, and recombinant proteins Tamoxifen Sigma Cat# T5648 Recommbinant mouse FGF10 R&D Systems Cat# 6224-FG Recommbinant human FGF10 R&D Systems Cat# 345-FG-025 thioacetamide Sigma Cat# 163678 Doxycycline hyclate Sigma Cat# D9891 Polyinosinic-Polycytidylic acid Sigma Cat# P1530 - Cellstain - DAPI solution DOJINDO Cat# D523 PD0325901 Selleck Chemicals Cat# S1036 L-arabinose Sigma Cat# A3256 Sucrose FUJIFILM Cat# 192-00017 dimethyl sulfoxide FUJIFILM Cat# 043-07211 (Continued on next page) Cell Reports 34, 108772, February 23, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydroxypropyl methylcellulose FUJIFILM Cat# 515-70211 Polyoxyethylene(20) Sorbitan Monolaurate FUJIFILM Cat# 165-11511 MEM with non-essential amino acids Thermo Fisher Scientific Cat# 11140-019, used in BEC media Insulin-transferrin-selenium Thermo Fisher Scientific Cat# 51300-044, used in BEC media Na Pyruvate Thermo Fisher Scientific Cat# 11360070, used in BEC media Chemically-defined lipid concentrate Thermo Fisher Scientific Cat# 11905-031, used in BEC media Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122, used in BEC media Gentamicin Thermo Fisher Scientific Cat# 15750-060, used in BEC media Ethanolamine Sigma Cat# E9508, used in BEC media MEM vitamin solution Thermo Fisher Scientific Cat# 11120-052, used in BEC media Soybean trypsin inhibitor FUJIFILM Cat# 208-09223, used in BEC media L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030149, used in BEC media Bovine pituitary extract Thermo Fisher Scientific Cat# 13028014, used in BEC media Dexamethasone Sigma Cat# D4902, used in BEC media 3 30,5-triiodo-L-thyronine Sigma Cat# 01-101, used in BEC media Epidermal growth factor Merck, Millipore Cat# 01-101, used in BEC media Amphotericin B Sigma Cat# F6886, used in BEC media 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 NVP-BGJ398 Chemscene Cat# CS-0586 ESGRO Recombinant Mouse LIF Protein Merck, Millipore Cat# ESG1107 Critical commercial assays Vectastain Universal Elite ABC kit (mouse IgG) Vector Laboratories Cat# PK-6102 Vectastain Universal Elite ABC kit (Rabbit IgG) Vector Laboratories Cat# PK-6101 SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat# 18080051 Rneasy Mini Kit QIAGEN Cat# 74104 RNAscope 2.5 HD Reagent Kit- BROWN ACD Cat# 322310 iPGell Genostaff Cat# PG20-1 Deposited data RNA microarray raw data This paper GEO: GSE 119227 Experimental Models: Cell Lines KH2 embryonic stem cells Beard et al., 2006 NA KBDC-11 cells (IPNB cells) Murakami et al., 2014 NA KKU-213 cells (papillary cholangiocarcinoma) JCRB cell bank Cat# JCRB1557 PA6 cells Riken Bioresource Center Cell Bank Cat# RCB1127 Experimental models: organisms/strains C57BL/6J The Jackson Laboratory Stock# 00064 CAnN.Cg-Foxn1nu/CrlCrlj (BALB/c-nu) Charles River Laboratories NA Krt19-iCre (BAC) Kanayama et al., 2019 Generated. ..



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