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murine 430a high density oligo nucleotide array  (Thermo Fisher)


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    Structured Review

    Thermo Fisher murine 430a high density oligo nucleotide array
    Murine 430a High Density Oligo Nucleotide Array, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+expression+430a+array+platform/Oligomycin/pmc02674782-353-11-10
    Average 99 stars, based on 1 article reviews
    murine 430a high density oligo nucleotide array - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Natural antisense transcript Nat9a suppresses Scn9a (Na V 1.7) expression in parvalbumin-positive proprioceptive and inhibitory neurons.
    Article Snippet: .. Total RNA was isolated 72 hours post-transfection, and reverse transcription was performed using oligo d(T) and Superscript III first-strand synthesis system (Invitrogen) according to the manufacturer’s conditions. ..

    Article Title: Sfrp5 protects acute myocardial infarction by inhibiting macrophage foam cell formation and apoptosis via modulation of macrophage polarization and p38-MAPK/JNK/NF-κB pathways.
    Article Snippet: RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyzer and RNA 6000 Nano LabChip Kit (Agilent Technologies, USA); samples with RNA integrity number (RIN) > 7.0 were used for subsequent library preparation. .. Polyadenylated mRNA was isolated from 5 μg of total RNA using two rounds of purification with Dynabeads Oligo(dT) (Thermo Fisher Scientific, USA). .. Purified mRNA was fragmented using divalent cations at elevated temperature (Magnesium RNA Jo ur na l P re -p ro of Fragmentation Module, New England Biolabs, USA) by incubating at 94°C for 5-7 min. First-strand cDNA was synthesized using SuperScript II Reverse Transcriptase (Invitrogen, USA), followed by second-strand synthesis incorporating dUTP using E. coli DNA polymerase I, RNase H (New England Biolabs, USA), and dUTP solution (Thermo Fisher Scientific, USA).

    Reverse Transcription:

    Article Title: Natural antisense transcript Nat9a suppresses Scn9a (Na V 1.7) expression in parvalbumin-positive proprioceptive and inhibitory neurons.
    Article Snippet: .. Total RNA was isolated 72 hours post-transfection, and reverse transcription was performed using oligo d(T) and Superscript III first-strand synthesis system (Invitrogen) according to the manufacturer’s conditions. ..

    Article Title: Re-engineering segment 8 facilitates generation of a versatile live-attenuated influenza A virus vector platform for secretory protein delivery.
    Article Snippet: HP Total RNA Kit (Omega Bio-tek) following the manufacturer’s guidelines. .. Resultant RNAs were reverse transcribed into complemen tary DNA with the Omniscript RT Kit (Qiagen) supplemented with Oligo(dT) primers (Invitrogen) according to the manufacturer’s instructions. .. Then, cDNAs were quantified by SYBR green-based qPCR using the LightCycler 480 SYBR Green I Master mix and specific primers (listed in Table S2) on a LightCycler 480 II qPCR system (Roche).

    Article Title: Uncoupling neocortical neuron fate and migration via a Let-7-RBX2 axis.
    Article Snippet: .. A 0.5 μg/μL pCAG- mCherry and 0.125 μg/μL pCAG-T7 -RBX2 mix was used to express RBX2 alone. pCAG-T7 -RBX2 expresses a T7- tagged mouse RBX2 cDNA, obtained from embryonic brain mRNA by a reverse transcription reaction (SuperScript II Reverse Transcriptase system, Invitrogen) using Oligo-dT (Invitrogen) primer and PCR amplification (Herculase II, Agilent Technologies). ..

    Article Title: Cytoplasmic localization of pseudouridine synthase 7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance.
    Article Snippet: Nucleic acids were collected by ethanol precipitation and treated with DNase I (Roche 04716728001), then precipitated again to yield total RNA. tRNA was prepared by separating total RNA via anion exchange chromatography on a Resource Q column (Cytiva). tRNA-containing fractions were pooled and concentrated by ethanol precipitation. .. Poly-A RNA enrichment of RNA was performed on total RNA using DynabeadsTM Oligo(dT) 25 (Thermo, 61005) according to the manufacturer’s recommended protocol. mRNA quantification by RT-qPCR: 2 μg total RNA was reverse transcribed with RevertAid First Strand cDNA Synthesis Kit (Thermo ScientificTM, K1622) using random hexamer primers to produce cDNA.1 μL of the produced cDNA was used to perform a RT-qPCR with Luminaris Color HiGreen qPCR Master Mix (Catalog number: K0392) according to the manufacturer’s protocol. ..

    Article Title: N-Acetylcysteine Prevents Arsenic-Induced Apoptosis but Not Supernumerary Motor Neuron Development in Zebrafish Embryos: Assessment of Protein Carbonylation and the p53 Pathway.
    Article Snippet: .. The first strand complementary DNA (cDNA) was synthesized from total RNA (4.5 μg; 20 μL final reaction volume) with oligo (dT) priming using SuperScript III reverse transcriptase (ThermoFisher, Waltham, MA, USA) according to the manufacturer’s instructions. .. Quantitative polymerase chain reaction (qPCR) analyses were performed using a CFX96 C1000 PCR system (Bio-Rad, Hercules, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Uncoupling neocortical neuron fate and migration via a Let-7-RBX2 axis.
    Article Snippet: .. A 0.5 μg/μL pCAG- mCherry and 0.125 μg/μL pCAG-T7 -RBX2 mix was used to express RBX2 alone. pCAG-T7 -RBX2 expresses a T7- tagged mouse RBX2 cDNA, obtained from embryonic brain mRNA by a reverse transcription reaction (SuperScript II Reverse Transcriptase system, Invitrogen) using Oligo-dT (Invitrogen) primer and PCR amplification (Herculase II, Agilent Technologies). ..

    Amplification:

    Article Title: Uncoupling neocortical neuron fate and migration via a Let-7-RBX2 axis.
    Article Snippet: .. A 0.5 μg/μL pCAG- mCherry and 0.125 μg/μL pCAG-T7 -RBX2 mix was used to express RBX2 alone. pCAG-T7 -RBX2 expresses a T7- tagged mouse RBX2 cDNA, obtained from embryonic brain mRNA by a reverse transcription reaction (SuperScript II Reverse Transcriptase system, Invitrogen) using Oligo-dT (Invitrogen) primer and PCR amplification (Herculase II, Agilent Technologies). ..

    Synthesized:

    Article Title: Wheat CENH3 alleles
    Article Snippet: .. Total RNA of juvenile leaves was extracted using INVITROGEN TRIzol following manufacturer's instructions. cDNA was synthesized from 1 mg of total RNA via Superscript III first-strand synthesis system (Invitrogen) with oligo-dT primer. .. KOD-Plus-Neo (TOYOBO) was used to amplify TaCenH3α-A transcripts with primers F1 (SEQ ID NO:54) and R1 (SEQ ID NO:55).

    Article Title: N-Acetylcysteine Prevents Arsenic-Induced Apoptosis but Not Supernumerary Motor Neuron Development in Zebrafish Embryos: Assessment of Protein Carbonylation and the p53 Pathway.
    Article Snippet: .. The first strand complementary DNA (cDNA) was synthesized from total RNA (4.5 μg; 20 μL final reaction volume) with oligo (dT) priming using SuperScript III reverse transcriptase (ThermoFisher, Waltham, MA, USA) according to the manufacturer’s instructions. .. Quantitative polymerase chain reaction (qPCR) analyses were performed using a CFX96 C1000 PCR system (Bio-Rad, Hercules, CA, USA).

    Purification:

    Article Title: Sfrp5 protects acute myocardial infarction by inhibiting macrophage foam cell formation and apoptosis via modulation of macrophage polarization and p38-MAPK/JNK/NF-κB pathways.
    Article Snippet: RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyzer and RNA 6000 Nano LabChip Kit (Agilent Technologies, USA); samples with RNA integrity number (RIN) > 7.0 were used for subsequent library preparation. .. Polyadenylated mRNA was isolated from 5 μg of total RNA using two rounds of purification with Dynabeads Oligo(dT) (Thermo Fisher Scientific, USA). .. Purified mRNA was fragmented using divalent cations at elevated temperature (Magnesium RNA Jo ur na l P re -p ro of Fragmentation Module, New England Biolabs, USA) by incubating at 94°C for 5-7 min. First-strand cDNA was synthesized using SuperScript II Reverse Transcriptase (Invitrogen, USA), followed by second-strand synthesis incorporating dUTP using E. coli DNA polymerase I, RNase H (New England Biolabs, USA), and dUTP solution (Thermo Fisher Scientific, USA).

    cDNA Synthesis:

    Article Title: Cytoplasmic localization of pseudouridine synthase 7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance.
    Article Snippet: Nucleic acids were collected by ethanol precipitation and treated with DNase I (Roche 04716728001), then precipitated again to yield total RNA. tRNA was prepared by separating total RNA via anion exchange chromatography on a Resource Q column (Cytiva). tRNA-containing fractions were pooled and concentrated by ethanol precipitation. .. Poly-A RNA enrichment of RNA was performed on total RNA using DynabeadsTM Oligo(dT) 25 (Thermo, 61005) according to the manufacturer’s recommended protocol. mRNA quantification by RT-qPCR: 2 μg total RNA was reverse transcribed with RevertAid First Strand cDNA Synthesis Kit (Thermo ScientificTM, K1622) using random hexamer primers to produce cDNA.1 μL of the produced cDNA was used to perform a RT-qPCR with Luminaris Color HiGreen qPCR Master Mix (Catalog number: K0392) according to the manufacturer’s protocol. ..

    Random Hexamer:

    Article Title: Cytoplasmic localization of pseudouridine synthase 7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance.
    Article Snippet: Nucleic acids were collected by ethanol precipitation and treated with DNase I (Roche 04716728001), then precipitated again to yield total RNA. tRNA was prepared by separating total RNA via anion exchange chromatography on a Resource Q column (Cytiva). tRNA-containing fractions were pooled and concentrated by ethanol precipitation. .. Poly-A RNA enrichment of RNA was performed on total RNA using DynabeadsTM Oligo(dT) 25 (Thermo, 61005) according to the manufacturer’s recommended protocol. mRNA quantification by RT-qPCR: 2 μg total RNA was reverse transcribed with RevertAid First Strand cDNA Synthesis Kit (Thermo ScientificTM, K1622) using random hexamer primers to produce cDNA.1 μL of the produced cDNA was used to perform a RT-qPCR with Luminaris Color HiGreen qPCR Master Mix (Catalog number: K0392) according to the manufacturer’s protocol. ..

    Produced:

    Article Title: Cytoplasmic localization of pseudouridine synthase 7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance.
    Article Snippet: Nucleic acids were collected by ethanol precipitation and treated with DNase I (Roche 04716728001), then precipitated again to yield total RNA. tRNA was prepared by separating total RNA via anion exchange chromatography on a Resource Q column (Cytiva). tRNA-containing fractions were pooled and concentrated by ethanol precipitation. .. Poly-A RNA enrichment of RNA was performed on total RNA using DynabeadsTM Oligo(dT) 25 (Thermo, 61005) according to the manufacturer’s recommended protocol. mRNA quantification by RT-qPCR: 2 μg total RNA was reverse transcribed with RevertAid First Strand cDNA Synthesis Kit (Thermo ScientificTM, K1622) using random hexamer primers to produce cDNA.1 μL of the produced cDNA was used to perform a RT-qPCR with Luminaris Color HiGreen qPCR Master Mix (Catalog number: K0392) according to the manufacturer’s protocol. ..

    Quantitative RT-PCR:

    Article Title: Cytoplasmic localization of pseudouridine synthase 7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance.
    Article Snippet: Nucleic acids were collected by ethanol precipitation and treated with DNase I (Roche 04716728001), then precipitated again to yield total RNA. tRNA was prepared by separating total RNA via anion exchange chromatography on a Resource Q column (Cytiva). tRNA-containing fractions were pooled and concentrated by ethanol precipitation. .. Poly-A RNA enrichment of RNA was performed on total RNA using DynabeadsTM Oligo(dT) 25 (Thermo, 61005) according to the manufacturer’s recommended protocol. mRNA quantification by RT-qPCR: 2 μg total RNA was reverse transcribed with RevertAid First Strand cDNA Synthesis Kit (Thermo ScientificTM, K1622) using random hexamer primers to produce cDNA.1 μL of the produced cDNA was used to perform a RT-qPCR with Luminaris Color HiGreen qPCR Master Mix (Catalog number: K0392) according to the manufacturer’s protocol. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Cytoplasmic localization of pseudouridine synthase 7 facilitates a pseudouridine-dependent enhancement of cellular stress tolerance.
    Article Snippet: Nucleic acids were collected by ethanol precipitation and treated with DNase I (Roche 04716728001), then precipitated again to yield total RNA. tRNA was prepared by separating total RNA via anion exchange chromatography on a Resource Q column (Cytiva). tRNA-containing fractions were pooled and concentrated by ethanol precipitation. .. Poly-A RNA enrichment of RNA was performed on total RNA using DynabeadsTM Oligo(dT) 25 (Thermo, 61005) according to the manufacturer’s recommended protocol. mRNA quantification by RT-qPCR: 2 μg total RNA was reverse transcribed with RevertAid First Strand cDNA Synthesis Kit (Thermo ScientificTM, K1622) using random hexamer primers to produce cDNA.1 μL of the produced cDNA was used to perform a RT-qPCR with Luminaris Color HiGreen qPCR Master Mix (Catalog number: K0392) according to the manufacturer’s protocol. ..

    Membrane:

    Article Title: PHYCUT: Scalable Multiplex CRISPR/Cas9 Editing for Genome Engineering in the Diatom Phaeodactylum tricornutum .
    Article Snippet: .. The membrane was prehybridized with prewarmed ULTRAhyb-oligo (Invitrogen) buffer at 42 °C for 30 min with gentle agitation in a glass tube. ..

    Gentle:

    Article Title: PHYCUT: Scalable Multiplex CRISPR/Cas9 Editing for Genome Engineering in the Diatom Phaeodactylum tricornutum .
    Article Snippet: .. The membrane was prehybridized with prewarmed ULTRAhyb-oligo (Invitrogen) buffer at 42 °C for 30 min with gentle agitation in a glass tube. ..



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