rabbit monoclonal antibody anti fbw7 (Proteintech)

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Proteintech rabbit monoclonal antibody anti fbw7

Rabbit Monoclonal Antibody Anti Fbw7, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+dna+methylation+microarray/FBXW7+Antibody/pmc11148513-11-0-5
Average 94 stars, based on 78 article reviews

rabbit monoclonal antibody anti fbw7 - by Bioz Stars, 2026-11

94/100 stars

Images

1) Product Images from "Arachidonic acid released by PIK3CA mutant tumor cells triggers malignant transformation of colonic epithelium by inducing chromatin remodeling"

Article Title: Arachidonic acid released by PIK3CA mutant tumor cells triggers malignant transformation of colonic epithelium by inducing chromatin remodeling

Journal: Cell Reports Medicine

doi: 10.1016/j.xcrm.2024.101510

Figure Legend Snippet:

Techniques Used: Microarray, Recombinant, Cloning, Mutagenesis, Immunohistochemistry, Protein Purification, In Vitro, Sequencing, Methylation

Molecular Weight:

Article Title: The KLKB1-TFE3-BRAF/MEK/ERK axis regulates neuronal ferroptosis in vascular dementia.
Article Snippet: .. Name Brand and article number Molecular weight (kDa) Dilution ratio ACSL4 Proteintech 22401-1-AP 74 1:2000 KLKB1 Proteintech 27991-1-AP 71 1:1000 GPX4 Proteintech 67763-1-Ig 20 1:5000 FBXO30 Proteintech 28424-1-AP 66 1:3000 TFE3 Proteintech 14480-1-AP 70 1:500 TFAM abclonal A3173 24 1:1000 GAPDH Proteintech 60004-1-Ig 36 1:10000 BRAF Proteintech 20899-1-AP 65 1:2000 MEK1/2 Proteintech 11049-1-AP 47 1:10000 p-MEK1/ 2 Affinity AF8035 45 1:2000 ERK1/2 Proteintech 11257-1-AP 44 1:8000 p-ERK1/2 Proteintech 28733-1-AP 38 1:3000 considered to indicate statistical significance. ..

Western Blot:

Article Title: Interaction of CSN4 to DDB1 regulates its stability and function in DNA damage signaling
Article Snippet: .. The following primary antibodies were used for immunoblotting: anti-DDB1, anti-FBXW7, anti-RFP, and anti-Ambra1 (Proteintech, USA); anti–β-actin, anti-Skp2, anti-Skp1, anti–phospho-Chk1, and anti-FLAG (Cell Signaling Technology, USA); anti-HA (Roche, Switzerland); anti–α-tubulin, anti-Chk1, anti-CSN6, and anti-ubiquitin (Santa Cruz Biotechnology, USA); anti-CSN4, anti-Cul4a, and anti-CSN5 (Bethyl Laboratories, USA); anti-DDB2 (Bosterbio, USA); anti-CSN6 (Santa Cruz Biotechnology, USA); and anti-SOCS2 (Abcam, UK). .. HRP-conjugated secondary antibodies (Bio-Rad, USA) were used, and signals were detected using enhanced chemiluminescence (ECL) reagents.

Proximity Ligation Assay:

Article Title: FBXW7-mediated CHK2 regulation modulates DNA damage response and cellular stability in Huntington’s disease
Article Snippet: B PLA shows FBXW7 (Abcam, Ab192328 , anti-rabbit) interacts with wtHTT (Millipore, Mab2166, anti-mouse) in HeLa cells ( n = 5). .. C PLA with mRFP-FBXW7 (RFP, Proteintech, anti-rabbit) and wtHTT (Millipore, Mab2166, anti-mouse) shows specific interaction with FBXW7 and wtHTT ( n = 5). ..

Cell-Signaling:

Article Title: Sorafenib promotes the E3 ubiquitin ligase FBXW7 to increase tau degradation and ameliorate tauopathies
Article Snippet: SH-SY5Y cells with tau(P301L)-EGFP expression were treated with 10 μmol/L of 54 FDA-approved drugs (Targetmol, Shanghai, China) that can cross the blood-brain barrier or the DMSO vehicle control for 24 h. Equal amounts of cell lysates were analyzed by immunoblotting for total tau and tau phosphorylated at Serine 404 (pS404) levels. .. Antibodies used in the present study including anti-FBXW7 (A5872, Abclonal, Wuhan, China), anti-FBXW7 (55290-1-AP, Proteintech, Wuhan, China), anti-total tau (tau5; AHB0042, Thermo Fisher Scientific), anti-tau pS199 (44734G, Thermo Fisher Scientific), anti-tau pS202/T205 (AT8; MN1020, Thermo Fisher Scientific), anti-tau pS396 (44752G, Thermo Fisher Scientific), anti-tau pT231 (A19710, Abclonal), anti-tau pT231/S235) (20473, Cell Signaling Technology, Danvers, MA, USA), anti-tau pT181 (12885, Cell Signaling Technology), anti-tau pS404 (20194, Cell Signaling Technology), anti-tau pS202 (11834, Cell Signaling Technology), anti-tau pS235 ( R25855 , Zen Bio, Chengdu, China), anti-LC3 (3868S, Cell Signaling Technology), anti-mTOR (2983, Cell Signaling Technology), anti-p-mTOR (Ser2448) (5536, Cell Signaling Technology), anti-S6 (2217, Cell Signaling Technology), anti-pS6 (5364, Cell Signaling Technology), anti-ERK1/2 (4695, Cell Signaling Technology), anti-pERK1/2 (4370, Cell Signaling Technology), anti-GSK3 β (51065-1-AP, Proteintech), anti-pGSK3 β (Y216, Y279) (44604G, Thermo Fisher Scientific), anti-p38 (9212, Cell Signaling Technology), anti-p-p38 (4511, Cell Signaling Technology), anti-CDK5 (sc-173, Santa Cruz Biotechnology, Dallas, TX, USA), anti-GFP (50430-2-AP, Proteintech), anti-flag (20543-1-AP, Proteintech), anti-flag (66008-4-Ig, Proteintech), anti-myc (2276S, Cell Signaling Technology), anti-myc (16286-1-AP, Proteintech), anti-HA (51064-2-AP, Proteintech), anti-Ub (PTM-1106RM, PTM biolabs, Hangzhou, China), anti-GFAP (3670, Cell Signaling Technology), anti-Tuj1 (sc-173, Biolegend, San Diego, CA, USA), anti-NeuN (94403, Cell Signaling Technology), anti-Iba1 (17198, Cell Signaling Technology), anti-GAPDH (ab0038, Abways, Shanghai, China), and anti- β -actin (AC026, Abclonal). .. Horseradish peroxidase-conjugated secondary antibodies were from ThermoFisher Scientific (31430 and 31460).

other:

Article Title: Interaction of CSN4 to DDB1 regulates its stability and function in DNA damage signaling.
Article Snippet: Lysates were incubated with anti-FLAG M2 affinity gel (Sigma-Aldrich, USA) or anti-HA magnetic beads for at least 3 h at 4 °C.

Review