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mouse α myh7 monoclonal  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse α myh7 monoclonal

    Mouse α Myh7 Monoclonal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+dna+methylation+microarray/MYH7+Antibody/pmc10261899-8-0-4
    Average 94 stars, based on 165 article reviews
    mouse α myh7 monoclonal - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Engineering CpG island DNA methylation in pluripotent cells through synthetic CpG-free ssDNA insertion"

    Article Title: Engineering CpG island DNA methylation in pluripotent cells through synthetic CpG-free ssDNA insertion

    Journal: Cell Reports Methods

    doi: 10.1016/j.crmeth.2023.100465


    Figure Legend Snippet:

    Techniques Used: Recombinant, Membrane, Transfection, SYBR Green Assay, DNA Methylation Assay, Mutagenesis, CRISPR, Software, Functional Assay, Microarray, In Vivo, Comparison, Next-Generation Sequencing

    Related Articles

    Staining:

    Article Title: Pathogenic TNNT1 variants are associated with aberrant thin filament compliance and myofibre hyper‐contractility
    Article Snippet: .. Briefly, flow‐chamber mounted myofibres were stained with an anti‐β‐cardiac/skeletal slow myosin heavy antibody (MYH7, IgG1, A4.951, sc‐53090 from Santa Cruz Biotechnology, Santa Cruz, CA, USA, dilution: 1:50). .. Myofibres were then washed in PBS/0.025% Tween‐20 and incubated with a secondary antibody: goat anti‐mouse IgG1 Alexa 555 (from ThermoScientific, Waltham, MA, USA, dilution 1:1000) in a blocking buffer.

    Incubation:

    Article Title: Pathogenic TNNT1 variants are associated with aberrant thin filament compliance and myofibre hyper‐contractility
    Article Snippet: Immunolabelling was performed on 10 μm cryosections, fixed in 4% paraformaldehyde (10 min), permeabilized in 0.1% Triton X‐100 (20 min) and blocked in 10% normal goat serum (50062Z, Life Technologies, Carlsbad, CA, USA) with 0.1% bovine serum albumin (BSA) (1 h). .. Sections were incubated overnight (4°C) with primary antibody against MYH7 (type I fibres; mouse monoclonal A4.951, Santa Cruz, sc‐53090, diluted 1:25) or MYH2 (type IIA fibres; mouse monoclonal SC71, DSHB, Iowa City, IA, USA 1:25), each combined with primary antibody against TNNT1 (rabbit polyclonal HPA058448, Sigma, St Louis, MO, USA, diluted 1:500), TNNT3 (rabbit polyclonal HPA037810, Sigma, diluted 1:500) or laminin (rabbit polyclonal, Sigma, L9393, diluted 1:20) in 5% goat serum with 0.1% BSA and 0.1% Triton X‐100. .. Alexa Fluor goat anti‐mouse 647 (A21237) was used as the secondary antibody for the MYHs and Alexa Fluor donkey anti‐rabbit 488 (A11034) for the TNNTs and laminin (Life Technologies, 1:500 each in 10% normal goat serum).



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