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anti asph mouse monoclonal antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti asph mouse monoclonal antibody
    Bioinformatics analysis of aspartate-β-hydroxylase <t>(ASPH)</t> overexpression in CRC: ( a ) analysis of ASPH mRNA expression in normal mucosa and colorectal cancer (CRC) samples from TCGA database (UALCAN website); ( b ) ASPH promoter methylation status in normal mucosa and CRC samples from TCGA database (UALCAN website; beta-value scale ranges from 0 = unmethylated to 1 = fully methylated); ( c ) analysis of ASPH protein levels by mass spectrometry in normal mucosa and CRC samples from the CPTAC database (UALCAN website; Z-values represent standard deviations from the median across the samples); ( d ) putative copy number alterations of the ASPH gene computed by GISTIC algorithm in TCGA database (c-BioPortal website; shallow deletion = 1− copy, gain = 1+ copy, amplification ≥ 2+). Box plots represent the quartile distribution of data around the median value. Statistics are reported in each graph as the calculated p value.
    Anti Asph Mouse Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+dna+methylation+microarray/ASPH+Antibody/pmc07226058-67-1-8
    Average 93 stars, based on 13 article reviews
    anti asph mouse monoclonal antibody - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Aspartate-β-Hydroxylase: A Promising Target to Limit the Local Invasiveness of Colorectal Cancer"

    Article Title: Aspartate-β-Hydroxylase: A Promising Target to Limit the Local Invasiveness of Colorectal Cancer

    Journal: Cancers

    doi: 10.3390/cancers12040971

    Bioinformatics analysis of aspartate-β-hydroxylase (ASPH) overexpression in CRC: ( a ) analysis of ASPH mRNA expression in normal mucosa and colorectal cancer (CRC) samples from TCGA database (UALCAN website); ( b ) ASPH promoter methylation status in normal mucosa and CRC samples from TCGA database (UALCAN website; beta-value scale ranges from 0 = unmethylated to 1 = fully methylated); ( c ) analysis of ASPH protein levels by mass spectrometry in normal mucosa and CRC samples from the CPTAC database (UALCAN website; Z-values represent standard deviations from the median across the samples); ( d ) putative copy number alterations of the ASPH gene computed by GISTIC algorithm in TCGA database (c-BioPortal website; shallow deletion = 1− copy, gain = 1+ copy, amplification ≥ 2+). Box plots represent the quartile distribution of data around the median value. Statistics are reported in each graph as the calculated p value.
    Figure Legend Snippet: Bioinformatics analysis of aspartate-β-hydroxylase (ASPH) overexpression in CRC: ( a ) analysis of ASPH mRNA expression in normal mucosa and colorectal cancer (CRC) samples from TCGA database (UALCAN website); ( b ) ASPH promoter methylation status in normal mucosa and CRC samples from TCGA database (UALCAN website; beta-value scale ranges from 0 = unmethylated to 1 = fully methylated); ( c ) analysis of ASPH protein levels by mass spectrometry in normal mucosa and CRC samples from the CPTAC database (UALCAN website; Z-values represent standard deviations from the median across the samples); ( d ) putative copy number alterations of the ASPH gene computed by GISTIC algorithm in TCGA database (c-BioPortal website; shallow deletion = 1− copy, gain = 1+ copy, amplification ≥ 2+). Box plots represent the quartile distribution of data around the median value. Statistics are reported in each graph as the calculated p value.

    Techniques Used: Over Expression, Expressing, Methylation, Mass Spectrometry, Amplification

    Heatmaps of mRNA-clusters significantly correlated with ASPH expression (red = high, green = low), selected in : ( a ) Immune signature ( n = 222); ( b ) Notch signature ( n = 203); ( c ) Invasive signature ( n = 203). Each cluster is ordered according to Pearson’s r coefficients of each marker against ASPH, from higher (left) to lower (right). The maximum r coefficient was 0.330 (SNW1), and the lowest was -0.304 (LFNG); thus, no marker showed a widespread correlation with ASPH among analyzed samples. Microarray Z -scores for the selected markers were extracted from the COAD Firehouse legacy database (TCGA) using the cBioPortal web interface.
    Figure Legend Snippet: Heatmaps of mRNA-clusters significantly correlated with ASPH expression (red = high, green = low), selected in : ( a ) Immune signature ( n = 222); ( b ) Notch signature ( n = 203); ( c ) Invasive signature ( n = 203). Each cluster is ordered according to Pearson’s r coefficients of each marker against ASPH, from higher (left) to lower (right). The maximum r coefficient was 0.330 (SNW1), and the lowest was -0.304 (LFNG); thus, no marker showed a widespread correlation with ASPH among analyzed samples. Microarray Z -scores for the selected markers were extracted from the COAD Firehouse legacy database (TCGA) using the cBioPortal web interface.

    Techniques Used: Expressing, Marker, Microarray

    ASPH expression in the tumor invasive margin (IM) correlates with the pattern of local invasion and patients’ overall survival: invasive front type (I = infiltrative, E = expansive), tumor budding (1 = 0–4 buds, 2 = 5–9 buds, 3 ≥ 10 buds) and overall survival (L = low, H = high ASPH levels dichotomized by the median value) show a strong relation only with ASPH levels scored in CRC IM. Center of the tumor (CT)-scored ASPH shows a statistically significant relation only with tumor budding, while ASPH expressed next to tumor lumen (LM) does not show any relation with these parameters. Box plots represent the quartile distribution of ASPH H-score quantifications in CRC samples around the median value. Statistics are reported in each graph as the calculated p value (Invasive front: Student t -test; Budding: one-way ANOVA; Overall survival: Kaplan–Meier analysis).
    Figure Legend Snippet: ASPH expression in the tumor invasive margin (IM) correlates with the pattern of local invasion and patients’ overall survival: invasive front type (I = infiltrative, E = expansive), tumor budding (1 = 0–4 buds, 2 = 5–9 buds, 3 ≥ 10 buds) and overall survival (L = low, H = high ASPH levels dichotomized by the median value) show a strong relation only with ASPH levels scored in CRC IM. Center of the tumor (CT)-scored ASPH shows a statistically significant relation only with tumor budding, while ASPH expressed next to tumor lumen (LM) does not show any relation with these parameters. Box plots represent the quartile distribution of ASPH H-score quantifications in CRC samples around the median value. Statistics are reported in each graph as the calculated p value (Invasive front: Student t -test; Budding: one-way ANOVA; Overall survival: Kaplan–Meier analysis).

    Techniques Used: Expressing

    ASPH silencing or activity inhibition decreases cell invasion and proliferation in a panel of CRC cell lines. Specific ASPH siRNAs visibly downregulate ASPH expression after 18 h ( a ). Concomitantly, cell invasion ( b ) and proliferation ( c ) are significantly inhibited. All these effects are associated with Notch1 inhibition ( d ). One representative experiment out of three is reported. β-Actin was utilized as a loading control. Means ± SEM are reported. *** p < 0.001. DLD1 and SW480 cell lines were exposed to increasing concentration of MO-I-1144. For the invasion test ( e ), filters were recovered and counted after 18 h. Representative images of filters from 20 µM treated cells are shown in ( f ). Cell growth was evaluated after 96 h by the crystal violet assay ( g ). One representative experiment (conducted on 10 replicates) out of three is reported. Mean ± SEM are reported. ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: ASPH silencing or activity inhibition decreases cell invasion and proliferation in a panel of CRC cell lines. Specific ASPH siRNAs visibly downregulate ASPH expression after 18 h ( a ). Concomitantly, cell invasion ( b ) and proliferation ( c ) are significantly inhibited. All these effects are associated with Notch1 inhibition ( d ). One representative experiment out of three is reported. β-Actin was utilized as a loading control. Means ± SEM are reported. *** p < 0.001. DLD1 and SW480 cell lines were exposed to increasing concentration of MO-I-1144. For the invasion test ( e ), filters were recovered and counted after 18 h. Representative images of filters from 20 µM treated cells are shown in ( f ). Cell growth was evaluated after 96 h by the crystal violet assay ( g ). One representative experiment (conducted on 10 replicates) out of three is reported. Mean ± SEM are reported. ** p < 0.01, *** p < 0.001.

    Techniques Used: Activity Assay, Inhibition, Expressing, Control, Concentration Assay, Crystal Violet Assay

    Related Articles

    Concentration Assay:

    Article Title: Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cells
    Article Snippet: .. Samples were blocked with 4% BSA for 3 h, and the primary antibody anti-HSP27 (1:200 concentration, ab2790) and anti-HSP70 mouse monoclonal (1:200, Santa Cruz Biotechnology, sc-32239) were incubated at 37 °C for 3 h. Subsequently, primary antibodies were removed by washing three times in PBS, and the goat anti-mouse secondary antibody, Alexa Fluor 488 (1:500, Invitrogen, A-11029), was incubated at 37 °C for 1 h. Anti-ASPH mouse monoclonal antibody conjugated to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-271391) was incubated at 37 °C for 1 h. Finally, each sample was washed thrice in PBS and mounted in FluorshieldTM with DAPI (4,6-diamidino-2-phenylindole) to stain nuclei. .. The images were performed using the Olympus FluoView FV1000 confocal laser scanning microscope (Olympus, Tokyo, Japan) with oil immersion objective lenses 60X.

    Article Title: Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cells.
    Article Snippet: .. Samples were blocked with 4% BSA for 3 h, and the primary antibody anti-HSP27 (1:200 concentration, ab2790) and anti-HSP70 mouse monoclonal (1:200, Santa Cruz Biotechnology, sc-32239) were incubated at 37 °C for 3 h. Subsequently, primary antibodies were removed by washing three times in PBS, and the goat anti-mouse secondary antibody, Alexa Fluor 488 (1:500, Invitrogen, A-11029), was incubated at 37 °C for 1 h. Anti-ASPH mouse monoclonal antibody conjugated to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-271391) was incubated at 37 °C for 1 h. Finally, each sample was washed thrice in PBS and mounted in FluorshieldTM with DAPI (4,6-diamidino-2-phenylindole) to stain nuclei. .. The images were performed using the Olympus FluoView FV1000 confocal laser scanning microscope (Olympus, Tokyo, Japan) with oil immersion objective lenses 60X.

    Incubation:

    Article Title: Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cells
    Article Snippet: .. Samples were blocked with 4% BSA for 3 h, and the primary antibody anti-HSP27 (1:200 concentration, ab2790) and anti-HSP70 mouse monoclonal (1:200, Santa Cruz Biotechnology, sc-32239) were incubated at 37 °C for 3 h. Subsequently, primary antibodies were removed by washing three times in PBS, and the goat anti-mouse secondary antibody, Alexa Fluor 488 (1:500, Invitrogen, A-11029), was incubated at 37 °C for 1 h. Anti-ASPH mouse monoclonal antibody conjugated to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-271391) was incubated at 37 °C for 1 h. Finally, each sample was washed thrice in PBS and mounted in FluorshieldTM with DAPI (4,6-diamidino-2-phenylindole) to stain nuclei. .. The images were performed using the Olympus FluoView FV1000 confocal laser scanning microscope (Olympus, Tokyo, Japan) with oil immersion objective lenses 60X.

    Article Title: Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cells.
    Article Snippet: .. Samples were blocked with 4% BSA for 3 h, and the primary antibody anti-HSP27 (1:200 concentration, ab2790) and anti-HSP70 mouse monoclonal (1:200, Santa Cruz Biotechnology, sc-32239) were incubated at 37 °C for 3 h. Subsequently, primary antibodies were removed by washing three times in PBS, and the goat anti-mouse secondary antibody, Alexa Fluor 488 (1:500, Invitrogen, A-11029), was incubated at 37 °C for 1 h. Anti-ASPH mouse monoclonal antibody conjugated to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-271391) was incubated at 37 °C for 1 h. Finally, each sample was washed thrice in PBS and mounted in FluorshieldTM with DAPI (4,6-diamidino-2-phenylindole) to stain nuclei. .. The images were performed using the Olympus FluoView FV1000 confocal laser scanning microscope (Olympus, Tokyo, Japan) with oil immersion objective lenses 60X.

    Article Title: 10-hydroxy-2-decenoic acid prevents osteoarthritis by targeting aspartyl β hydroxylase and inhibiting chondrocyte senescence in male mice preclinically
    Article Snippet: .. Fixed paraffin section of human cartilage tissue or mice knee joints with 4% paraformaldehyde (Solarbio Life Sciences, Cat. P1110), permeated with 0.5% TritonX-100 (Solarbio Life Sciences, Cat. IT9100), and incubated with the primary antibody: COL2 (Proteintech, Cat. 28459-1-AP, 1:100 dilution), ACAN (Proteintech, Cat. 13880-1-AP, 1:100 dilution), MMP13 (Proteintech, Cat. 18165-1-AP, 1:100 dilution), ASPH (Santa Cruz Biotechnology, Cat. sc-271391, 1:100 dilution), p16 (Proteintech, Cat. 10883-1-AP, 1:100 dilution), p21 (Proteintech, Cat. 10355-1-AP, 1:100 dilution) at 4 °C overnight. .. Incubated the secondary antibody (CoraLite488-conjugated Goat Anti-Mouse IgG(H + L), Proteintech, Cat. SA00013-1, 1:200 dilution; CoraLite488-conjugated Goat Anti-Rabbit IgG (H + L), Proteintech, Cat. SA00013-2, 1:200 dilution; Proteintech, CoraLite594-conjugated Goat Anti-Rabbit IgG (H + L), Cat. SA00013-4, 1:200 dilution) for 4 h and DAPI (Solarbio Life Sciences, Cat. C0065) for 5 min at room temperature.

    Article Title: Nanomodified Nexavar Enhances Efficacy in Caco-2 Cells via Targeting Aspartate β-Hydroxylase-Driven Mitochondrial Cell Death
    Article Snippet: .. Subsequently, membranes were incubated with a mouse https://doi.org/10.3390/immuno6010005 monoclonal anti-ASPH antibody (F-7 clone, Santa Cruz, CA, USA), which recognizes the active 86 kDa form of ASPH corresponding to amino acids 382–681 [34]. .. Following two washes with WesternBreeze solution (16×, Invitrogen, USA), membranes were incubated for 2 h at room temperature with mouse monoclonal anti-β-actin (A2228, Sigma, Hamburg, Germany) as a loading control.

    Article Title: 10-hydroxy-2-decenoic acid prevents osteoarthritis by targeting aspartyl β hydroxylase and inhibiting chondrocyte senescence in male mice preclinically.
    Article Snippet: .. Immunofluorescence Fixed paraffin section of human cartilage tissue or mice knee joints with 4% paraformaldehyde (Solarbio Life Sciences, Cat. P1110), permeatedwith0.5%TritonX-100 (Solarbio Life Sciences, Cat. IT9100), and incubated with the primary antibody: COL2 (Proteintech, Cat. 28459-1-AP, 1:100 dilution), ACAN (Proteintech, Cat. 13880-1-AP, 1:100 dilution), MMP13 (Proteintech, Cat. 18165-1-AP, 1:100 dilution), ASPH (Santa Cruz Biotechnology, Cat. sc-271391, 1:100 dilution), p16 (Proteintech, Cat. 10883-1-AP, 1:100 dilution), p21 (Proteintech, Cat. 10355- 1-AP, 1:100 dilution) at 4 °C overnight. .. Incubated the secondary antibody (CoraLite488-conjugated Goat Anti-Mouse IgG(H+ L), Proteintech, Cat. SA00013-1, 1:200 dilution; CoraLite488-conjugated Goat Anti-Rabbit IgG (H + L), Proteintech, Cat. SA00013-2, 1:200 dilution; Proteintech, CoraLite594-conjugatedGoat Anti-Rabbit IgG (H + L), Cat. SA00013-4, 1:200 dilution) for 4 h and DAPI (Solarbio Life Sciences, Cat. C0065) for 5min at room temperature.

    Staining:

    Article Title: Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cells
    Article Snippet: .. Samples were blocked with 4% BSA for 3 h, and the primary antibody anti-HSP27 (1:200 concentration, ab2790) and anti-HSP70 mouse monoclonal (1:200, Santa Cruz Biotechnology, sc-32239) were incubated at 37 °C for 3 h. Subsequently, primary antibodies were removed by washing three times in PBS, and the goat anti-mouse secondary antibody, Alexa Fluor 488 (1:500, Invitrogen, A-11029), was incubated at 37 °C for 1 h. Anti-ASPH mouse monoclonal antibody conjugated to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-271391) was incubated at 37 °C for 1 h. Finally, each sample was washed thrice in PBS and mounted in FluorshieldTM with DAPI (4,6-diamidino-2-phenylindole) to stain nuclei. .. The images were performed using the Olympus FluoView FV1000 confocal laser scanning microscope (Olympus, Tokyo, Japan) with oil immersion objective lenses 60X.

    Article Title: Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cells.
    Article Snippet: .. Samples were blocked with 4% BSA for 3 h, and the primary antibody anti-HSP27 (1:200 concentration, ab2790) and anti-HSP70 mouse monoclonal (1:200, Santa Cruz Biotechnology, sc-32239) were incubated at 37 °C for 3 h. Subsequently, primary antibodies were removed by washing three times in PBS, and the goat anti-mouse secondary antibody, Alexa Fluor 488 (1:500, Invitrogen, A-11029), was incubated at 37 °C for 1 h. Anti-ASPH mouse monoclonal antibody conjugated to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-271391) was incubated at 37 °C for 1 h. Finally, each sample was washed thrice in PBS and mounted in FluorshieldTM with DAPI (4,6-diamidino-2-phenylindole) to stain nuclei. .. The images were performed using the Olympus FluoView FV1000 confocal laser scanning microscope (Olympus, Tokyo, Japan) with oil immersion objective lenses 60X.

    Paraffin Section:

    Article Title: 10-hydroxy-2-decenoic acid prevents osteoarthritis by targeting aspartyl β hydroxylase and inhibiting chondrocyte senescence in male mice preclinically
    Article Snippet: .. Fixed paraffin section of human cartilage tissue or mice knee joints with 4% paraformaldehyde (Solarbio Life Sciences, Cat. P1110), permeated with 0.5% TritonX-100 (Solarbio Life Sciences, Cat. IT9100), and incubated with the primary antibody: COL2 (Proteintech, Cat. 28459-1-AP, 1:100 dilution), ACAN (Proteintech, Cat. 13880-1-AP, 1:100 dilution), MMP13 (Proteintech, Cat. 18165-1-AP, 1:100 dilution), ASPH (Santa Cruz Biotechnology, Cat. sc-271391, 1:100 dilution), p16 (Proteintech, Cat. 10883-1-AP, 1:100 dilution), p21 (Proteintech, Cat. 10355-1-AP, 1:100 dilution) at 4 °C overnight. .. Incubated the secondary antibody (CoraLite488-conjugated Goat Anti-Mouse IgG(H + L), Proteintech, Cat. SA00013-1, 1:200 dilution; CoraLite488-conjugated Goat Anti-Rabbit IgG (H + L), Proteintech, Cat. SA00013-2, 1:200 dilution; Proteintech, CoraLite594-conjugated Goat Anti-Rabbit IgG (H + L), Cat. SA00013-4, 1:200 dilution) for 4 h and DAPI (Solarbio Life Sciences, Cat. C0065) for 5 min at room temperature.

    Article Title: 10-hydroxy-2-decenoic acid prevents osteoarthritis by targeting aspartyl β hydroxylase and inhibiting chondrocyte senescence in male mice preclinically.
    Article Snippet: .. Immunofluorescence Fixed paraffin section of human cartilage tissue or mice knee joints with 4% paraformaldehyde (Solarbio Life Sciences, Cat. P1110), permeatedwith0.5%TritonX-100 (Solarbio Life Sciences, Cat. IT9100), and incubated with the primary antibody: COL2 (Proteintech, Cat. 28459-1-AP, 1:100 dilution), ACAN (Proteintech, Cat. 13880-1-AP, 1:100 dilution), MMP13 (Proteintech, Cat. 18165-1-AP, 1:100 dilution), ASPH (Santa Cruz Biotechnology, Cat. sc-271391, 1:100 dilution), p16 (Proteintech, Cat. 10883-1-AP, 1:100 dilution), p21 (Proteintech, Cat. 10355- 1-AP, 1:100 dilution) at 4 °C overnight. .. Incubated the secondary antibody (CoraLite488-conjugated Goat Anti-Mouse IgG(H+ L), Proteintech, Cat. SA00013-1, 1:200 dilution; CoraLite488-conjugated Goat Anti-Rabbit IgG (H + L), Proteintech, Cat. SA00013-2, 1:200 dilution; Proteintech, CoraLite594-conjugatedGoat Anti-Rabbit IgG (H + L), Cat. SA00013-4, 1:200 dilution) for 4 h and DAPI (Solarbio Life Sciences, Cat. C0065) for 5min at room temperature.

    Immunofluorescence:

    Article Title: 10-hydroxy-2-decenoic acid prevents osteoarthritis by targeting aspartyl β hydroxylase and inhibiting chondrocyte senescence in male mice preclinically.
    Article Snippet: .. Immunofluorescence Fixed paraffin section of human cartilage tissue or mice knee joints with 4% paraformaldehyde (Solarbio Life Sciences, Cat. P1110), permeatedwith0.5%TritonX-100 (Solarbio Life Sciences, Cat. IT9100), and incubated with the primary antibody: COL2 (Proteintech, Cat. 28459-1-AP, 1:100 dilution), ACAN (Proteintech, Cat. 13880-1-AP, 1:100 dilution), MMP13 (Proteintech, Cat. 18165-1-AP, 1:100 dilution), ASPH (Santa Cruz Biotechnology, Cat. sc-271391, 1:100 dilution), p16 (Proteintech, Cat. 10883-1-AP, 1:100 dilution), p21 (Proteintech, Cat. 10355- 1-AP, 1:100 dilution) at 4 °C overnight. .. Incubated the secondary antibody (CoraLite488-conjugated Goat Anti-Mouse IgG(H+ L), Proteintech, Cat. SA00013-1, 1:200 dilution; CoraLite488-conjugated Goat Anti-Rabbit IgG (H + L), Proteintech, Cat. SA00013-2, 1:200 dilution; Proteintech, CoraLite594-conjugatedGoat Anti-Rabbit IgG (H + L), Cat. SA00013-4, 1:200 dilution) for 4 h and DAPI (Solarbio Life Sciences, Cat. C0065) for 5min at room temperature.



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