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mouse dectin 1 antibody  (R&D Systems)


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    Structured Review

    R&D Systems mouse dectin 1 antibody
    Mouse Dectin 1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+dectin/Mouse+Dectin-1%2FCLEC7A+Antibody/pm41135901-54-0-7
    Average 93 stars, based on 15 article reviews
    mouse dectin 1 antibody - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: Identification of novel protein biomarkers of macrophage polarization using comparative proteomic analyses of murine primary macrophages
    Article Snippet: .. Primary antibodies used in this study included antibodies against glyceraldehyde-3-phosphate dehydrogenase (Gapdh; Cell Signaling, Boston, MA, USA, 2118; 1:1,000); C-C motif chemokine ligand 5 (Ccl5; Proteintech, Rosemont, IL, USA, 12000-1-AP; 1:500); mouse C-type lectin domain family 4, member e (Clec4e; Novus Biologicals, Centennial, CO, USA, NBP2-27093SS; 1:250); human CLEC4E (Proteintech, 19376-1-AP; 1:1,000); C-type lectin domain family 7, member a (Clec7a; R&D Systems, Minneapolis, MN, USA, AF1756-SP; 1:2,000); mouse CD72 antigen (Cd72; R&D Systems, AF1279SP; 1:1,000); guanylate binding protein 2 (Gbp2; Proteintech, 11854-1-AP; 1:1,000); mouse aconitate decarboxylase 1 (Acod1; Cell Signaling, 19857T; 1:1,000); human ACOD1 (Cell Signaling, 77510T; 1:1,000); and neurochondrin (Ncdn; Proteintech, 13187-1-AP; 1:1000). ..

    Binding Assay:

    Article Title: Identification of novel protein biomarkers of macrophage polarization using comparative proteomic analyses of murine primary macrophages
    Article Snippet: .. Primary antibodies used in this study included antibodies against glyceraldehyde-3-phosphate dehydrogenase (Gapdh; Cell Signaling, Boston, MA, USA, 2118; 1:1,000); C-C motif chemokine ligand 5 (Ccl5; Proteintech, Rosemont, IL, USA, 12000-1-AP; 1:500); mouse C-type lectin domain family 4, member e (Clec4e; Novus Biologicals, Centennial, CO, USA, NBP2-27093SS; 1:250); human CLEC4E (Proteintech, 19376-1-AP; 1:1,000); C-type lectin domain family 7, member a (Clec7a; R&D Systems, Minneapolis, MN, USA, AF1756-SP; 1:2,000); mouse CD72 antigen (Cd72; R&D Systems, AF1279SP; 1:1,000); guanylate binding protein 2 (Gbp2; Proteintech, 11854-1-AP; 1:1,000); mouse aconitate decarboxylase 1 (Acod1; Cell Signaling, 19857T; 1:1,000); human ACOD1 (Cell Signaling, 77510T; 1:1,000); and neurochondrin (Ncdn; Proteintech, 13187-1-AP; 1:1000). ..

    Article Title: Identification of novel protein biomarkers of macrophage polarization using comparative proteomic analyses of murine primary macrophages.
    Article Snippet: Principal component analysis (PCA) and heatmap diagrams were generated using Metware Cloud (https://cloud.metware.cn) to illustrate the distributions of the proteome profiles. .. Immunoblot and quantitative PCR (qPCR) analyses were carried out as previously described.15 Primary antibodies used in this study included antibodies against glyceraldehyde-3phosphate dehydrogenase (Gapdh; Cell Signaling, Boston, MA, USA, 2118; 1:1,000); C-C motif chemokine ligand 5 (Ccl5; Proteintech, Rosemont, IL, USA, 12000-1-AP; 1:500); mouse C-type lectin domain family 4, member e (Clec4e; Novus Biologicals, Centennial, CO, USA, NBP2-27093SS; 1:250); human CLEC4E (Proteintech, 19376-1-AP; 1:1,000); C-type lectin domain family 7, member a (Clec7a; R&D Systems, Minneapolis, MN, USA, AF1756-SP; 1:2,000); mouse CD72 antigen (Cd72; R&D Systems, AF1279SP; 1:1,000); guanylate binding protein 2 (Gbp2; Proteintech, 11854-1-AP; 1:1,000); mouse aconitate decarboxylase 1 (Acod1; Cell Signaling, 19857T; 1:1,000); human ACOD1 (Cell Signaling, 77510T; 1:1,000); and neurochondrin (Ncdn; Proteintech, 13187-1-AP; 1:1000). .. Immunoblot and quantitative PCR (qPCR) analyses were carried out as previously described.15 Primary antibodies used in this study included antibodies against glyceraldehyde-3phosphate dehydrogenase (Gapdh; Cell Signaling, Boston, MA, USA, 2118; 1:1,000); C-C motif chemokine ligand 5 (Ccl5; Proteintech, Rosemont, IL, USA, 12000-1-AP; 1:500); mouse C-type lectin domain family 4, member e (Clec4e; Novus Biologicals, Centennial, CO, USA, NBP2-27093SS; 1:250); human CLEC4E (Proteintech, 19376-1-AP; 1:1,000); C-type lectin domain family 7, member a (Clec7a; R&D Systems, Minneapolis, MN, USA, AF1756-SP; 1:2,000); mouse CD72 antigen (Cd72; R&D Systems, AF1279SP; 1:1,000); guanylate binding protein 2 (Gbp2; Proteintech, 11854-1-AP; 1:1,000); mouse aconitate decarboxylase 1 (Acod1; Cell Signaling, 19857T; 1:1,000); human ACOD1 (Cell Signaling, 77510T; 1:1,000); and neurochondrin (Ncdn; Proteintech, 13187-1-AP; 1:1000).

    Concentration Assay:

    Article Title: The lipid raft-associated protein stomatin is required for accumulation of dectin-1 in the phagosomal membrane and for full activity of macrophages against Aspergillus fumigatus
    Article Snippet: Macrophages and isolated phagosomes were blocked with 5 % (v/v) normal goat serum (Thermo Fisher Scientific), 2 % (w/v) BSA, 0.3 M glycine/PBS for 30 min before overnight incubation at 4 °C with the primary antibody in 2 % (w/v) BSA/PBS. .. As primary antibodies, rabbit anti-Stomatin (ab166623, Abcam), rabbit anti-ATP6V1B2 (ab73404, Abcam) or goat anti-dectin-1/CLEC7 (AF1756, R&D Systems) were used with a concentration of 1:500 (Stomatin, vATPase) or 1:100 (dectin-1). .. The incubation with secondary antibodies, goat anti-rabbit IgG DyLight 633 (35562, Thermo Fisher Scientific) or donkey anti-goat Alexa Fluor 488 (A11055, Life Technologies) was carried out at room temperature for 1 h with concentrations of 1:500 or 1:200.

    Western Blot:

    Article Title: Identification of novel protein biomarkers of macrophage polarization using comparative proteomic analyses of murine primary macrophages.
    Article Snippet: Principal component analysis (PCA) and heatmap diagrams were generated using Metware Cloud (https://cloud.metware.cn) to illustrate the distributions of the proteome profiles. .. Immunoblot and quantitative PCR (qPCR) analyses were carried out as previously described.15 Primary antibodies used in this study included antibodies against glyceraldehyde-3phosphate dehydrogenase (Gapdh; Cell Signaling, Boston, MA, USA, 2118; 1:1,000); C-C motif chemokine ligand 5 (Ccl5; Proteintech, Rosemont, IL, USA, 12000-1-AP; 1:500); mouse C-type lectin domain family 4, member e (Clec4e; Novus Biologicals, Centennial, CO, USA, NBP2-27093SS; 1:250); human CLEC4E (Proteintech, 19376-1-AP; 1:1,000); C-type lectin domain family 7, member a (Clec7a; R&D Systems, Minneapolis, MN, USA, AF1756-SP; 1:2,000); mouse CD72 antigen (Cd72; R&D Systems, AF1279SP; 1:1,000); guanylate binding protein 2 (Gbp2; Proteintech, 11854-1-AP; 1:1,000); mouse aconitate decarboxylase 1 (Acod1; Cell Signaling, 19857T; 1:1,000); human ACOD1 (Cell Signaling, 77510T; 1:1,000); and neurochondrin (Ncdn; Proteintech, 13187-1-AP; 1:1000). .. Immunoblot and quantitative PCR (qPCR) analyses were carried out as previously described.15 Primary antibodies used in this study included antibodies against glyceraldehyde-3phosphate dehydrogenase (Gapdh; Cell Signaling, Boston, MA, USA, 2118; 1:1,000); C-C motif chemokine ligand 5 (Ccl5; Proteintech, Rosemont, IL, USA, 12000-1-AP; 1:500); mouse C-type lectin domain family 4, member e (Clec4e; Novus Biologicals, Centennial, CO, USA, NBP2-27093SS; 1:250); human CLEC4E (Proteintech, 19376-1-AP; 1:1,000); C-type lectin domain family 7, member a (Clec7a; R&D Systems, Minneapolis, MN, USA, AF1756-SP; 1:2,000); mouse CD72 antigen (Cd72; R&D Systems, AF1279SP; 1:1,000); guanylate binding protein 2 (Gbp2; Proteintech, 11854-1-AP; 1:1,000); mouse aconitate decarboxylase 1 (Acod1; Cell Signaling, 19857T; 1:1,000); human ACOD1 (Cell Signaling, 77510T; 1:1,000); and neurochondrin (Ncdn; Proteintech, 13187-1-AP; 1:1000).

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of novel protein biomarkers of macrophage polarization using comparative proteomic analyses of murine primary macrophages.
    Article Snippet: Principal component analysis (PCA) and heatmap diagrams were generated using Metware Cloud (https://cloud.metware.cn) to illustrate the distributions of the proteome profiles. .. Immunoblot and quantitative PCR (qPCR) analyses were carried out as previously described.15 Primary antibodies used in this study included antibodies against glyceraldehyde-3phosphate dehydrogenase (Gapdh; Cell Signaling, Boston, MA, USA, 2118; 1:1,000); C-C motif chemokine ligand 5 (Ccl5; Proteintech, Rosemont, IL, USA, 12000-1-AP; 1:500); mouse C-type lectin domain family 4, member e (Clec4e; Novus Biologicals, Centennial, CO, USA, NBP2-27093SS; 1:250); human CLEC4E (Proteintech, 19376-1-AP; 1:1,000); C-type lectin domain family 7, member a (Clec7a; R&D Systems, Minneapolis, MN, USA, AF1756-SP; 1:2,000); mouse CD72 antigen (Cd72; R&D Systems, AF1279SP; 1:1,000); guanylate binding protein 2 (Gbp2; Proteintech, 11854-1-AP; 1:1,000); mouse aconitate decarboxylase 1 (Acod1; Cell Signaling, 19857T; 1:1,000); human ACOD1 (Cell Signaling, 77510T; 1:1,000); and neurochondrin (Ncdn; Proteintech, 13187-1-AP; 1:1000). .. Immunoblot and quantitative PCR (qPCR) analyses were carried out as previously described.15 Primary antibodies used in this study included antibodies against glyceraldehyde-3phosphate dehydrogenase (Gapdh; Cell Signaling, Boston, MA, USA, 2118; 1:1,000); C-C motif chemokine ligand 5 (Ccl5; Proteintech, Rosemont, IL, USA, 12000-1-AP; 1:500); mouse C-type lectin domain family 4, member e (Clec4e; Novus Biologicals, Centennial, CO, USA, NBP2-27093SS; 1:250); human CLEC4E (Proteintech, 19376-1-AP; 1:1,000); C-type lectin domain family 7, member a (Clec7a; R&D Systems, Minneapolis, MN, USA, AF1756-SP; 1:2,000); mouse CD72 antigen (Cd72; R&D Systems, AF1279SP; 1:1,000); guanylate binding protein 2 (Gbp2; Proteintech, 11854-1-AP; 1:1,000); mouse aconitate decarboxylase 1 (Acod1; Cell Signaling, 19857T; 1:1,000); human ACOD1 (Cell Signaling, 77510T; 1:1,000); and neurochondrin (Ncdn; Proteintech, 13187-1-AP; 1:1000).

    other:

    Article Title: Role of Dectin-1 in peripheral nerve injury
    Article Snippet: Dectin-1 , AF1756 , R and D Systems, Minneapolis, MN, United states , 1:500.



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    Image Search Results


    LPS from H. alvei strain BA2000346 activates both TLR4 and Dectin-2. ( A ) HEK-Blue TM human TLR (hTLR4) and ( B ) mouse Dectin-2 (mDectin-2) reporter cells were stimulated with LPS from E. coli O111:B4 or H. alvei BA2000346 for 24 h. Reporter activity was measured by absorbance at 655 nm. Data are shown as mean ± SD ( n = 6). Statistical significance was assessed using the Mann–Whitney test; ** p < 0.01. Comparisons were made against the negative control for hTLR4 and against E. coli O111:B4 for mDectin-2.

    Journal: Vaccines

    Article Title: Immunogenicity of an Oil-in-Water Emulsion Containing Hafnia Alvei -Derived Lipopolysaccharide, with TLR4 and Dectin-2 Agonist Activity In Vitro

    doi: 10.3390/vaccines14070557

    Figure Lengend Snippet: LPS from H. alvei strain BA2000346 activates both TLR4 and Dectin-2. ( A ) HEK-Blue TM human TLR (hTLR4) and ( B ) mouse Dectin-2 (mDectin-2) reporter cells were stimulated with LPS from E. coli O111:B4 or H. alvei BA2000346 for 24 h. Reporter activity was measured by absorbance at 655 nm. Data are shown as mean ± SD ( n = 6). Statistical significance was assessed using the Mann–Whitney test; ** p < 0.01. Comparisons were made against the negative control for hTLR4 and against E. coli O111:B4 for mDectin-2.

    Article Snippet: HEK-Blue TM human TLR4 (hTLR4) and mouse Dectin-2 (mDectin-2) reporter cells (InvivoGen) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, high glucose) supplemented with 10% FBS and 1% P/S.

    Techniques: Activity Assay, MANN-WHITNEY, Negative Control