Marker:Article Title: Mitochondrial Intercellular Transfer via Platelets After Physical Training Exerts Neuro‐Glial Protection Against Cerebral Ischemia
Article Snippet: .. After additional PBS washes, cells were blocked with 2% Blockace in PBS for 1 h. The following primary antibodies were used: goat polyclonal anti‐C3d (1:50; R&D Systems; AF2655), rabbit polyclonal anti‐S100A10 (1:100; Proteintech; 11250‐1‐AP), rabbit polyclonal anti‐PDGFRα (1:100; Santa Cruz Biotechnology; sc‐338), and mouse monoclonal anti‐myelin basic protein (MBP; OLG marker, 1:50; Millipore; MAB382). .. Following incubation with primary antibodies, cells were washed with PBS containing 0.2% Tween‐20 and incubated with appropriate secondary antibodies (1:200; Jackson ImmunoResearch Laboratories) for 1 h at room temperature.
Article Title: Mitochondrial Intercellular Transfer via Platelets After Physical Training Exerts Neuro‐Glial Protection Against Cerebral Ischemia
Article Snippet: Sections were blocked with 2% Blockace (Dainippon Sumitomo Pharma, Osaka, Japan; UK‐B80) for 1 h at room temperature, followed by overnight incubation at 4°C with primary antibodies. .. The following primary antibodies were used: mouse monoclonal anti‐GFAP (astrocyte marker; 1:200, Santa Cruz Biotechnology; sc‐33673), rabbit polyclonal anti‐GST‐pi (OLG marker; 1:200, MBL; code 312), mouse monoclonal anti‐Olig1 (OLG marker; 1:100, Millipore; MAB5540), mouse monoclonal anti‐CNPase (OLG marker; 1:100, Abcam; ab6319), rabbit polyclonal anti‐PDGFRα (OPC marker; 1:100, Santa Cruz Biotechnology; sc‐338), mouse monoclonal anti‐PDGFRα (1:100, Santa Cruz Biotechnology; sc‐398206), mouse monoclonal anti‐CD140a (OPC marker; 1:100, BD Biosciences; 558774), goat polyclonal anti‐C3d (inflammatory astrocyte marker; 1:50, R&D Systems; AF2655), rabbit polyclonal anti‐S100A10 (protective astrocyte marker; 1:100, Proteintech; 11250‐1‐AP), mouse monoclonal anti‐NeuN (neuronal marker; 1:100, Millipore; MAB377), mouse monoclonal anti‐SMI31 (axonal marker; 1:100, BioLegend; 801601), rabbit polyclonal anti‐Iba1 (microglial marker; 1:200, Wako; 019–19741), rat monoclonal anti‐CD31 (endothelial marker; 1:100, BD Pharmingen; 550274), and mouse monoclonal anti‐TOM20 (mitochondrial marker; 1:100, BD Transduction Laboratories; 612278). .. After washing with PBS, sections were incubated with appropriate secondary antibodies (1:250, Jackson ImmunoResearch Laboratories) for 1 h at room temperature, followed by mounting with VECTASHIELD containing DAPI (Vector Laboratories; H‐1200).
Article Title: Mitochondrial Intercellular Transfer via Platelets After Physical Training Exerts Neuro‐Glial Protection Against Cerebral Ischemia
Article Snippet: .. The following primary antibodies were used: mouse monoclonal anti‐GFAP (1:2000; Santa Cruz Biotechnology; sc‐33673), goat polyclonal anti‐C3d (1:1000; R&D Systems; AF2655), rabbit polyclonal anti‐S100A10 (1:2000; Proteintech; 11250‐1‐AP), mouse monoclonal anti‐MBP (1:2000; Thermo Scientific; MAB382), rabbit polyclonal anti‐PDGFRα (1:2000; Santa Cruz Biotechnology; sc‐398206), mouse monoclonal anti‐TOM20 (1:1000; BD Transduction Laboratories; 612278), rabbit polyclonal anti‐SOD2 (1:5000; proteintech; 24127‐1‐AP), mouse monoclonal anti‐NeuN (neuronal marker, 1:2000; Millipore; MAB377), mouse monoclonal anti‐SMI31 (axonal marker, 1:2000; BioLegend; 801601), rabbit polyclonal anti‐Iba‐1 (1:2000; Wako; 019–19741), and mouse monoclonal anti‐β‐actin (1:10000; Abcam; ab8226). .. Following overnight incubation at 4°C with primary antibodies, membranes were washed and incubated with HRP‐conjugated secondary antibodies (1:5000; Santa Cruz Biotechnology).
Western Blot:Article Title: Pharmacological inhibition of SPAK-NKCC1 complex attenuates astrogliosis and restores cerebral blood flow in a mouse model of VCID
Article Snippet: .. Application tNKCC1 Mouse 1:100 DSHB T4 IF pNKCC1 Rabbit 1:200 EMD Millipore ABS1004 GFAP Rabbit 1:100 Agilent Dako Z0334 Iba-1 Rabbit 1:200 FUJIFILM NC9288364 Iba-1 Mouse 1:100 Millipore Sigma MABN92 NG-2 Rabbit 1:200 EMD Millipore AB5320 APC Mouse 1:100 Millipore Sigma CALBIOCHEM OP80 NeuN Rabbit 1:500 Abcam AB177487 C3d Goat 1:100 R&D system AF2655 Claudin-5 Rabbit 1:100 Thermo Fisher Scientific 34-1600 Albumin Rabbit 1:200 Abcam AB19196 VEGFA Rabbit 1:200 Abcam Ab52917 MBP Rabbit 1:500 Abcam ab40390 SMI32 Mouse 1:1000 BioLegend 801701 GFAP Mouse 1:200 Cell Signaling 3670S LCN2 Rat 1:100 Abcam ab70287 AQP4 Rabbit 1:100 Proteintech 16473-1AP MMP2 Rabbit 1:100 Proteintech 10373-2-AP MMP9 Rabbit 1:100 Proteintech 10375-2-AP ZO-1 Rabbit 1:100 Thermo Fisher Scientific 617300 GLUT1 Mouse 1:100 Abcam AB40084 Tomato lectin 488 1:100 VectorLabs DL-1174 Tomato lectin 594 1:100 VectorLabs DL-1177 SYP Mouse 1:8000 Santa Cruz sc-17750 WB PSD-95 Rabbit 1:2000 Abcam ab18258 WB ..
Blocking Assay:Article Title: Complement component C6 deficiency exacerbates colorectal tumorigenesis by abolishing membrane attack complex formation and potentiating M2-like tumor-associated macrophage responses.
Article Snippet: .. After blocking with 1 % bovine serum albumin (BSA), sections were then incubated with goat anti-mouse C3d antibody (1:50; R&D Systems Cat# AF2655, RRID: AB_2066622, Minneapolis, MN), rabbit anti-SC5b-9 neoantigen (1:200; Complement Technology Cat# A227, RRID: AB_3697003, Tyler, TX), or rabbit anti-Ki-67 antibody (1 μg/ml; Abcam Cat# ab15580, RRID: AB_443209, Cambridge, MA) at 4 ◦C overnight. .. After rinses in PBS, the sections were incubated with HRP-conjugated rabbit anti-goat IgG H&L (1:200; Abcam Cat# ab97100, RRID: AB_10687752) or goat anti-rabbit IgG H&L (1:200; Abcam Cat# ab97051, RRID: AB_10679369) at room temperature for 1 h. A GTVision III immunohistochemical detection kit (GK500705; Gene Tech, Shanghai, China) was used to detect the immunoreactivity.
Incubation:Article Title: Complement component C6 deficiency exacerbates colorectal tumorigenesis by abolishing membrane attack complex formation and potentiating M2-like tumor-associated macrophage responses.
Article Snippet: .. After blocking with 1 % bovine serum albumin (BSA), sections were then incubated with goat anti-mouse C3d antibody (1:50; R&D Systems Cat# AF2655, RRID: AB_2066622, Minneapolis, MN), rabbit anti-SC5b-9 neoantigen (1:200; Complement Technology Cat# A227, RRID: AB_3697003, Tyler, TX), or rabbit anti-Ki-67 antibody (1 μg/ml; Abcam Cat# ab15580, RRID: AB_443209, Cambridge, MA) at 4 ◦C overnight. .. After rinses in PBS, the sections were incubated with HRP-conjugated rabbit anti-goat IgG H&L (1:200; Abcam Cat# ab97100, RRID: AB_10687752) or goat anti-rabbit IgG H&L (1:200; Abcam Cat# ab97051, RRID: AB_10679369) at room temperature for 1 h. A GTVision III immunohistochemical detection kit (GK500705; Gene Tech, Shanghai, China) was used to detect the immunoreactivity.
Virus:Article Title: Equilibrative nucleoside transporter 2 modulates inosine catabolism to influence astrocyte metabolism and reactivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies NeuN, Mouse monoclonal antibody Millipore Cat# MAB377_1:500; RRID: AB_2298772 S100β, Rabbit polyclonal antibody Dako Cat# Z0311_1:500; RRID: AB_10013383 GFAP, Mouse monoclonal antibody Sigma Aldrich Cat# G3893_1:500; RRID: AB_477010 Iba1, Mouse monoclonal antibody GeneTex Cat# GTX632426_1:500; RRID: AB_2888314 A 2A R, Guinea pig polyclonal antibody Frontier Institute Cat# MSFR100090_1:200; RRID: AB_2571656 Lcn2, Goat polyclonal antibody R&D system Cat# AF1857_1:200; RRID: AB_355022 C3, Goat polyclonal antibody R&D system Cat# AF2655_1:3000; RRID: AB_2066622 Actin, Mouse monoclonal antibody Sigma-Aldrich Cat# A2066_1:2000; RRID: AB_476693 Bacterial and virus strains AAV8 package AAV Core Facility of Institute of Biomedical Sciences, Academia Sinica, Taiwan https://www.ibms.sinica. edu.tw/aav/price.html Chemicals, peptides, and recombinant proteins Mouse TNFα Sigma Cat# T7539-50UG Mouse IL1α Sigma Cat# I5396-25UG Forodesine MERCK Cat# L13961-5MG NBTI (NBMPR) Sigma-Aldrich Cat# N2255 Dipyradimle Sigma-Aldrich Cat# D9766 3 H-glutamate PerkinElmer Cat# NET490250UC 3 H-Adenosine PerkinElmer Cat# NET1161250UC Hoechst-33258 Invitrogen Cat# H3569 Critical commercial assays myelin removal beads II MACS Cat# 130-092-628 anti-ACSA2 microbeads MACS Cat# 130-092-678 anti-CD11b microbeads MACS Cat# 130-093-634 biotin-labeled nonneuronal antibody cocktail MACS Cat# 130-115-390 PureLink RNA Micro kit Invitrogen Cat# 12183-016 Ovation® PicoSL WTA system V2 Nugene Cat# 3312 MitoProbeTM JC-1 Assay Kit Molecular Probes Cat# M34152 Inosine Quantification Assay Kit (Fluorometric) Abcam Cat# ab126286 Fluorometric Purine Nucleoside Phosphorylase Activity Assay Kit Abcam Cat# ab204706 ATP Determination Kit Molecular Probes Cat# A22066 The Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103015-100 The Seahorse XF Cell Glycolysis Stress Test Kit Agilent Cat# 103020-100 PierceTM BCA Assay Kit Thermo Fisher Cat# 23225 Deposited data The metabolomic data have been deposited in the NMDR under project ID PR002624 and study ID ST004164. ..
Bioprocessing:Article Title: Equilibrative nucleoside transporter 2 modulates inosine catabolism to influence astrocyte metabolism and reactivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies NeuN, Mouse monoclonal antibody Millipore Cat# MAB377_1:500; RRID: AB_2298772 S100β, Rabbit polyclonal antibody Dako Cat# Z0311_1:500; RRID: AB_10013383 GFAP, Mouse monoclonal antibody Sigma Aldrich Cat# G3893_1:500; RRID: AB_477010 Iba1, Mouse monoclonal antibody GeneTex Cat# GTX632426_1:500; RRID: AB_2888314 A 2A R, Guinea pig polyclonal antibody Frontier Institute Cat# MSFR100090_1:200; RRID: AB_2571656 Lcn2, Goat polyclonal antibody R&D system Cat# AF1857_1:200; RRID: AB_355022 C3, Goat polyclonal antibody R&D system Cat# AF2655_1:3000; RRID: AB_2066622 Actin, Mouse monoclonal antibody Sigma-Aldrich Cat# A2066_1:2000; RRID: AB_476693 Bacterial and virus strains AAV8 package AAV Core Facility of Institute of Biomedical Sciences, Academia Sinica, Taiwan https://www.ibms.sinica. edu.tw/aav/price.html Chemicals, peptides, and recombinant proteins Mouse TNFα Sigma Cat# T7539-50UG Mouse IL1α Sigma Cat# I5396-25UG Forodesine MERCK Cat# L13961-5MG NBTI (NBMPR) Sigma-Aldrich Cat# N2255 Dipyradimle Sigma-Aldrich Cat# D9766 3 H-glutamate PerkinElmer Cat# NET490250UC 3 H-Adenosine PerkinElmer Cat# NET1161250UC Hoechst-33258 Invitrogen Cat# H3569 Critical commercial assays myelin removal beads II MACS Cat# 130-092-628 anti-ACSA2 microbeads MACS Cat# 130-092-678 anti-CD11b microbeads MACS Cat# 130-093-634 biotin-labeled nonneuronal antibody cocktail MACS Cat# 130-115-390 PureLink RNA Micro kit Invitrogen Cat# 12183-016 Ovation® PicoSL WTA system V2 Nugene Cat# 3312 MitoProbeTM JC-1 Assay Kit Molecular Probes Cat# M34152 Inosine Quantification Assay Kit (Fluorometric) Abcam Cat# ab126286 Fluorometric Purine Nucleoside Phosphorylase Activity Assay Kit Abcam Cat# ab204706 ATP Determination Kit Molecular Probes Cat# A22066 The Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103015-100 The Seahorse XF Cell Glycolysis Stress Test Kit Agilent Cat# 103020-100 PierceTM BCA Assay Kit Thermo Fisher Cat# 23225 Deposited data The metabolomic data have been deposited in the NMDR under project ID PR002624 and study ID ST004164. ..
Recombinant:Article Title: Equilibrative nucleoside transporter 2 modulates inosine catabolism to influence astrocyte metabolism and reactivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies NeuN, Mouse monoclonal antibody Millipore Cat# MAB377_1:500; RRID: AB_2298772 S100β, Rabbit polyclonal antibody Dako Cat# Z0311_1:500; RRID: AB_10013383 GFAP, Mouse monoclonal antibody Sigma Aldrich Cat# G3893_1:500; RRID: AB_477010 Iba1, Mouse monoclonal antibody GeneTex Cat# GTX632426_1:500; RRID: AB_2888314 A 2A R, Guinea pig polyclonal antibody Frontier Institute Cat# MSFR100090_1:200; RRID: AB_2571656 Lcn2, Goat polyclonal antibody R&D system Cat# AF1857_1:200; RRID: AB_355022 C3, Goat polyclonal antibody R&D system Cat# AF2655_1:3000; RRID: AB_2066622 Actin, Mouse monoclonal antibody Sigma-Aldrich Cat# A2066_1:2000; RRID: AB_476693 Bacterial and virus strains AAV8 package AAV Core Facility of Institute of Biomedical Sciences, Academia Sinica, Taiwan https://www.ibms.sinica. edu.tw/aav/price.html Chemicals, peptides, and recombinant proteins Mouse TNFα Sigma Cat# T7539-50UG Mouse IL1α Sigma Cat# I5396-25UG Forodesine MERCK Cat# L13961-5MG NBTI (NBMPR) Sigma-Aldrich Cat# N2255 Dipyradimle Sigma-Aldrich Cat# D9766 3 H-glutamate PerkinElmer Cat# NET490250UC 3 H-Adenosine PerkinElmer Cat# NET1161250UC Hoechst-33258 Invitrogen Cat# H3569 Critical commercial assays myelin removal beads II MACS Cat# 130-092-628 anti-ACSA2 microbeads MACS Cat# 130-092-678 anti-CD11b microbeads MACS Cat# 130-093-634 biotin-labeled nonneuronal antibody cocktail MACS Cat# 130-115-390 PureLink RNA Micro kit Invitrogen Cat# 12183-016 Ovation® PicoSL WTA system V2 Nugene Cat# 3312 MitoProbeTM JC-1 Assay Kit Molecular Probes Cat# M34152 Inosine Quantification Assay Kit (Fluorometric) Abcam Cat# ab126286 Fluorometric Purine Nucleoside Phosphorylase Activity Assay Kit Abcam Cat# ab204706 ATP Determination Kit Molecular Probes Cat# A22066 The Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103015-100 The Seahorse XF Cell Glycolysis Stress Test Kit Agilent Cat# 103020-100 PierceTM BCA Assay Kit Thermo Fisher Cat# 23225 Deposited data The metabolomic data have been deposited in the NMDR under project ID PR002624 and study ID ST004164. ..
Magnetic Cell Separation:Article Title: Equilibrative nucleoside transporter 2 modulates inosine catabolism to influence astrocyte metabolism and reactivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies NeuN, Mouse monoclonal antibody Millipore Cat# MAB377_1:500; RRID: AB_2298772 S100β, Rabbit polyclonal antibody Dako Cat# Z0311_1:500; RRID: AB_10013383 GFAP, Mouse monoclonal antibody Sigma Aldrich Cat# G3893_1:500; RRID: AB_477010 Iba1, Mouse monoclonal antibody GeneTex Cat# GTX632426_1:500; RRID: AB_2888314 A 2A R, Guinea pig polyclonal antibody Frontier Institute Cat# MSFR100090_1:200; RRID: AB_2571656 Lcn2, Goat polyclonal antibody R&D system Cat# AF1857_1:200; RRID: AB_355022 C3, Goat polyclonal antibody R&D system Cat# AF2655_1:3000; RRID: AB_2066622 Actin, Mouse monoclonal antibody Sigma-Aldrich Cat# A2066_1:2000; RRID: AB_476693 Bacterial and virus strains AAV8 package AAV Core Facility of Institute of Biomedical Sciences, Academia Sinica, Taiwan https://www.ibms.sinica. edu.tw/aav/price.html Chemicals, peptides, and recombinant proteins Mouse TNFα Sigma Cat# T7539-50UG Mouse IL1α Sigma Cat# I5396-25UG Forodesine MERCK Cat# L13961-5MG NBTI (NBMPR) Sigma-Aldrich Cat# N2255 Dipyradimle Sigma-Aldrich Cat# D9766 3 H-glutamate PerkinElmer Cat# NET490250UC 3 H-Adenosine PerkinElmer Cat# NET1161250UC Hoechst-33258 Invitrogen Cat# H3569 Critical commercial assays myelin removal beads II MACS Cat# 130-092-628 anti-ACSA2 microbeads MACS Cat# 130-092-678 anti-CD11b microbeads MACS Cat# 130-093-634 biotin-labeled nonneuronal antibody cocktail MACS Cat# 130-115-390 PureLink RNA Micro kit Invitrogen Cat# 12183-016 Ovation® PicoSL WTA system V2 Nugene Cat# 3312 MitoProbeTM JC-1 Assay Kit Molecular Probes Cat# M34152 Inosine Quantification Assay Kit (Fluorometric) Abcam Cat# ab126286 Fluorometric Purine Nucleoside Phosphorylase Activity Assay Kit Abcam Cat# ab204706 ATP Determination Kit Molecular Probes Cat# A22066 The Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103015-100 The Seahorse XF Cell Glycolysis Stress Test Kit Agilent Cat# 103020-100 PierceTM BCA Assay Kit Thermo Fisher Cat# 23225 Deposited data The metabolomic data have been deposited in the NMDR under project ID PR002624 and study ID ST004164. ..
Activity Assay:Article Title: Equilibrative nucleoside transporter 2 modulates inosine catabolism to influence astrocyte metabolism and reactivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies NeuN, Mouse monoclonal antibody Millipore Cat# MAB377_1:500; RRID: AB_2298772 S100β, Rabbit polyclonal antibody Dako Cat# Z0311_1:500; RRID: AB_10013383 GFAP, Mouse monoclonal antibody Sigma Aldrich Cat# G3893_1:500; RRID: AB_477010 Iba1, Mouse monoclonal antibody GeneTex Cat# GTX632426_1:500; RRID: AB_2888314 A 2A R, Guinea pig polyclonal antibody Frontier Institute Cat# MSFR100090_1:200; RRID: AB_2571656 Lcn2, Goat polyclonal antibody R&D system Cat# AF1857_1:200; RRID: AB_355022 C3, Goat polyclonal antibody R&D system Cat# AF2655_1:3000; RRID: AB_2066622 Actin, Mouse monoclonal antibody Sigma-Aldrich Cat# A2066_1:2000; RRID: AB_476693 Bacterial and virus strains AAV8 package AAV Core Facility of Institute of Biomedical Sciences, Academia Sinica, Taiwan https://www.ibms.sinica. edu.tw/aav/price.html Chemicals, peptides, and recombinant proteins Mouse TNFα Sigma Cat# T7539-50UG Mouse IL1α Sigma Cat# I5396-25UG Forodesine MERCK Cat# L13961-5MG NBTI (NBMPR) Sigma-Aldrich Cat# N2255 Dipyradimle Sigma-Aldrich Cat# D9766 3 H-glutamate PerkinElmer Cat# NET490250UC 3 H-Adenosine PerkinElmer Cat# NET1161250UC Hoechst-33258 Invitrogen Cat# H3569 Critical commercial assays myelin removal beads II MACS Cat# 130-092-628 anti-ACSA2 microbeads MACS Cat# 130-092-678 anti-CD11b microbeads MACS Cat# 130-093-634 biotin-labeled nonneuronal antibody cocktail MACS Cat# 130-115-390 PureLink RNA Micro kit Invitrogen Cat# 12183-016 Ovation® PicoSL WTA system V2 Nugene Cat# 3312 MitoProbeTM JC-1 Assay Kit Molecular Probes Cat# M34152 Inosine Quantification Assay Kit (Fluorometric) Abcam Cat# ab126286 Fluorometric Purine Nucleoside Phosphorylase Activity Assay Kit Abcam Cat# ab204706 ATP Determination Kit Molecular Probes Cat# A22066 The Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103015-100 The Seahorse XF Cell Glycolysis Stress Test Kit Agilent Cat# 103020-100 PierceTM BCA Assay Kit Thermo Fisher Cat# 23225 Deposited data The metabolomic data have been deposited in the NMDR under project ID PR002624 and study ID ST004164. ..
BIA-KA:Article Title: Equilibrative nucleoside transporter 2 modulates inosine catabolism to influence astrocyte metabolism and reactivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies NeuN, Mouse monoclonal antibody Millipore Cat# MAB377_1:500; RRID: AB_2298772 S100β, Rabbit polyclonal antibody Dako Cat# Z0311_1:500; RRID: AB_10013383 GFAP, Mouse monoclonal antibody Sigma Aldrich Cat# G3893_1:500; RRID: AB_477010 Iba1, Mouse monoclonal antibody GeneTex Cat# GTX632426_1:500; RRID: AB_2888314 A 2A R, Guinea pig polyclonal antibody Frontier Institute Cat# MSFR100090_1:200; RRID: AB_2571656 Lcn2, Goat polyclonal antibody R&D system Cat# AF1857_1:200; RRID: AB_355022 C3, Goat polyclonal antibody R&D system Cat# AF2655_1:3000; RRID: AB_2066622 Actin, Mouse monoclonal antibody Sigma-Aldrich Cat# A2066_1:2000; RRID: AB_476693 Bacterial and virus strains AAV8 package AAV Core Facility of Institute of Biomedical Sciences, Academia Sinica, Taiwan https://www.ibms.sinica. edu.tw/aav/price.html Chemicals, peptides, and recombinant proteins Mouse TNFα Sigma Cat# T7539-50UG Mouse IL1α Sigma Cat# I5396-25UG Forodesine MERCK Cat# L13961-5MG NBTI (NBMPR) Sigma-Aldrich Cat# N2255 Dipyradimle Sigma-Aldrich Cat# D9766 3 H-glutamate PerkinElmer Cat# NET490250UC 3 H-Adenosine PerkinElmer Cat# NET1161250UC Hoechst-33258 Invitrogen Cat# H3569 Critical commercial assays myelin removal beads II MACS Cat# 130-092-628 anti-ACSA2 microbeads MACS Cat# 130-092-678 anti-CD11b microbeads MACS Cat# 130-093-634 biotin-labeled nonneuronal antibody cocktail MACS Cat# 130-115-390 PureLink RNA Micro kit Invitrogen Cat# 12183-016 Ovation® PicoSL WTA system V2 Nugene Cat# 3312 MitoProbeTM JC-1 Assay Kit Molecular Probes Cat# M34152 Inosine Quantification Assay Kit (Fluorometric) Abcam Cat# ab126286 Fluorometric Purine Nucleoside Phosphorylase Activity Assay Kit Abcam Cat# ab204706 ATP Determination Kit Molecular Probes Cat# A22066 The Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103015-100 The Seahorse XF Cell Glycolysis Stress Test Kit Agilent Cat# 103020-100 PierceTM BCA Assay Kit Thermo Fisher Cat# 23225 Deposited data The metabolomic data have been deposited in the NMDR under project ID PR002624 and study ID ST004164. ..
Metabolomic:Article Title: Equilibrative nucleoside transporter 2 modulates inosine catabolism to influence astrocyte metabolism and reactivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies NeuN, Mouse monoclonal antibody Millipore Cat# MAB377_1:500; RRID: AB_2298772 S100β, Rabbit polyclonal antibody Dako Cat# Z0311_1:500; RRID: AB_10013383 GFAP, Mouse monoclonal antibody Sigma Aldrich Cat# G3893_1:500; RRID: AB_477010 Iba1, Mouse monoclonal antibody GeneTex Cat# GTX632426_1:500; RRID: AB_2888314 A 2A R, Guinea pig polyclonal antibody Frontier Institute Cat# MSFR100090_1:200; RRID: AB_2571656 Lcn2, Goat polyclonal antibody R&D system Cat# AF1857_1:200; RRID: AB_355022 C3, Goat polyclonal antibody R&D system Cat# AF2655_1:3000; RRID: AB_2066622 Actin, Mouse monoclonal antibody Sigma-Aldrich Cat# A2066_1:2000; RRID: AB_476693 Bacterial and virus strains AAV8 package AAV Core Facility of Institute of Biomedical Sciences, Academia Sinica, Taiwan https://www.ibms.sinica. edu.tw/aav/price.html Chemicals, peptides, and recombinant proteins Mouse TNFα Sigma Cat# T7539-50UG Mouse IL1α Sigma Cat# I5396-25UG Forodesine MERCK Cat# L13961-5MG NBTI (NBMPR) Sigma-Aldrich Cat# N2255 Dipyradimle Sigma-Aldrich Cat# D9766 3 H-glutamate PerkinElmer Cat# NET490250UC 3 H-Adenosine PerkinElmer Cat# NET1161250UC Hoechst-33258 Invitrogen Cat# H3569 Critical commercial assays myelin removal beads II MACS Cat# 130-092-628 anti-ACSA2 microbeads MACS Cat# 130-092-678 anti-CD11b microbeads MACS Cat# 130-093-634 biotin-labeled nonneuronal antibody cocktail MACS Cat# 130-115-390 PureLink RNA Micro kit Invitrogen Cat# 12183-016 Ovation® PicoSL WTA system V2 Nugene Cat# 3312 MitoProbeTM JC-1 Assay Kit Molecular Probes Cat# M34152 Inosine Quantification Assay Kit (Fluorometric) Abcam Cat# ab126286 Fluorometric Purine Nucleoside Phosphorylase Activity Assay Kit Abcam Cat# ab204706 ATP Determination Kit Molecular Probes Cat# A22066 The Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103015-100 The Seahorse XF Cell Glycolysis Stress Test Kit Agilent Cat# 103020-100 PierceTM BCA Assay Kit Thermo Fisher Cat# 23225 Deposited data The metabolomic data have been deposited in the NMDR under project ID PR002624 and study ID ST004164. ..
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