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anti cd4 pe  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti cd4 pe
    Anti Cd4 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd4/CD4+Antibody%2C+anti-mouse%2C+REAfinity/pmc13355016-7-0-2
    Average 95 stars, based on 122 article reviews
    anti cd4 pe - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: CCL signaling drives T Cell–Macrophage crosstalk in the mouse colon during chronic Trypanosoma cruzi infection
    Article Snippet: anti-CD4-PE , Miltenyi Biotec , Cat-130-116-509, RRID: AB_2727582.

    Activation Assay:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Expressing:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Isolation:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Staining:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Flow Cytometry:

    Article Title: Monocyte infiltration induces CNS arginine catabolism to fuel neuroinflammation.
    Article Snippet: .. Flow cytometry and cell sorting For analysis of spinal cord cell populations, stainings were performed in PBS using fixable viability dye (Invitrogen, 65-0865-14, 1:2,000, 10 min at room temperature) as well as the following antibodies: CD45.2-BV650 (clone 104, Biolegend, 109836, 1:100), Ly6G-BV605 (clone 1A8, Biolegend, 127639, 1:100), Ly6G-AF700 (clone 1A8, Biolegend, 127622, 1:100), CD3-BV605 (clone 145-2C11, Biolegend, 100351, 1:100), Ly6C-BV605 (clone HK14, Biolegend, 128035, 1:100), Ly6C-BV510 (clone HK14, Biolegend, 128033, 1:100), CD11b-BV510 (clone M1/70, Biolegend, 101245, 1:100), CD11b-PB (clone M1/70.15, Invitrogen, RM2828, 1:100), CD11b-BV605 (clone M1/70, Biolegend, 101237, 1:100), CX3CR1-PeCy7 (clone SA011F11, Biolegend, 149015, 1:100), CCR2-BV421 (clone SA203G11, Biolegend, 150605, 1:100), CCR2-FITC (clone SA203G11, Biolegend, 150607, 1:100), F4/80-BV510 (clone BM8, Biolegend, 123135, 1:100), F4/80-BV605 (clone BM8, Biolegend, 123133, 1:100), F4/80-FITC (clone BM8, Biolegend, 123108, 1:100), CD11c-PE-Cy5.5 (clone N418, Invitrogen, 35-0114-82, 1:100), P2RY12-APC (clone S16007D, Biolegend, 848006, 1:100), P2RY12-AF488 (clone S16007D, Biolegend, 848016, 1:100), MerTK-APC (clone 2B10C42, Biolegend, 151507, 1:100), CD44-FITC (clone IM7, Invitrogen, 11-0441-82, 1:100), CD44-eF506 (clone IM7, Invitrogen, 69-0441-82, 1:100), CD64-BV605 (clone X545/7.1, Biolegend, 139323, 1:100), CD3-AF700 (clone 17A2, Biolegend, 100216, 1:100), CD3-rF710 (clone 17A2, Tonbo, 80-0032-U100, 1:80), CD8-BV510 (clone 53-6.7, Biolegend, 100751, 1:80), CD4-APC (clone REA604, Miltenyi Biotec, 130-116-487, 1:80), CD4-PE (clone REA604, Miltenyi Biotec, 130-116-509, 1:80) and CD25-BV421 (clone PC61, Biolegend, 102033, 1:80). ..

    FACS:

    Article Title: Monocyte infiltration induces CNS arginine catabolism to fuel neuroinflammation.
    Article Snippet: .. Flow cytometry and cell sorting For analysis of spinal cord cell populations, stainings were performed in PBS using fixable viability dye (Invitrogen, 65-0865-14, 1:2,000, 10 min at room temperature) as well as the following antibodies: CD45.2-BV650 (clone 104, Biolegend, 109836, 1:100), Ly6G-BV605 (clone 1A8, Biolegend, 127639, 1:100), Ly6G-AF700 (clone 1A8, Biolegend, 127622, 1:100), CD3-BV605 (clone 145-2C11, Biolegend, 100351, 1:100), Ly6C-BV605 (clone HK14, Biolegend, 128035, 1:100), Ly6C-BV510 (clone HK14, Biolegend, 128033, 1:100), CD11b-BV510 (clone M1/70, Biolegend, 101245, 1:100), CD11b-PB (clone M1/70.15, Invitrogen, RM2828, 1:100), CD11b-BV605 (clone M1/70, Biolegend, 101237, 1:100), CX3CR1-PeCy7 (clone SA011F11, Biolegend, 149015, 1:100), CCR2-BV421 (clone SA203G11, Biolegend, 150605, 1:100), CCR2-FITC (clone SA203G11, Biolegend, 150607, 1:100), F4/80-BV510 (clone BM8, Biolegend, 123135, 1:100), F4/80-BV605 (clone BM8, Biolegend, 123133, 1:100), F4/80-FITC (clone BM8, Biolegend, 123108, 1:100), CD11c-PE-Cy5.5 (clone N418, Invitrogen, 35-0114-82, 1:100), P2RY12-APC (clone S16007D, Biolegend, 848006, 1:100), P2RY12-AF488 (clone S16007D, Biolegend, 848016, 1:100), MerTK-APC (clone 2B10C42, Biolegend, 151507, 1:100), CD44-FITC (clone IM7, Invitrogen, 11-0441-82, 1:100), CD44-eF506 (clone IM7, Invitrogen, 69-0441-82, 1:100), CD64-BV605 (clone X545/7.1, Biolegend, 139323, 1:100), CD3-AF700 (clone 17A2, Biolegend, 100216, 1:100), CD3-rF710 (clone 17A2, Tonbo, 80-0032-U100, 1:80), CD8-BV510 (clone 53-6.7, Biolegend, 100751, 1:80), CD4-APC (clone REA604, Miltenyi Biotec, 130-116-487, 1:80), CD4-PE (clone REA604, Miltenyi Biotec, 130-116-509, 1:80) and CD25-BV421 (clone PC61, Biolegend, 102033, 1:80). ..



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    Image Search Results


    ORFV-specific antibody responses and CD4 + and CD8 + T cell responses. ( A ) Kinetics of ORFV-specific antibody responses measured by ELISA after intramuscular immunization with F1L mRNA LNPs (5, 10, 15 μg), commercial live vaccine (CV), or PBS control on days 0 and 14. ( B ) Neutralizing antibody titers (NT50) in sera collected on day 28, determined by microneutralization assay (ORFV FZ strain, 100 TCID50; BHK-21 cells). Data are mean ± SD with individual points (n = 10 per group). * p < 0.05, ** p < 0.01 vs. PBS; ns, not significant ( p > 0.05); one-way ANOVA with Dunnett’s posttest. ( C , D ) Frequency of CD4 + ( C ) and CD8 + . ( D ) T cells following the same immunization regimen as in ( A , B ). Statistical comparisons as in ( B ).

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: An ORFV F1L mRNA Vaccine Candidate: Preparation, Immunogenicity, and Comparison with a Commercial Live Vaccine

    doi: 10.3390/ani16142274

    Figure Lengend Snippet: ORFV-specific antibody responses and CD4 + and CD8 + T cell responses. ( A ) Kinetics of ORFV-specific antibody responses measured by ELISA after intramuscular immunization with F1L mRNA LNPs (5, 10, 15 μg), commercial live vaccine (CV), or PBS control on days 0 and 14. ( B ) Neutralizing antibody titers (NT50) in sera collected on day 28, determined by microneutralization assay (ORFV FZ strain, 100 TCID50; BHK-21 cells). Data are mean ± SD with individual points (n = 10 per group). * p < 0.05, ** p < 0.01 vs. PBS; ns, not significant ( p > 0.05); one-way ANOVA with Dunnett’s posttest. ( C , D ) Frequency of CD4 + ( C ) and CD8 + . ( D ) T cells following the same immunization regimen as in ( A , B ). Statistical comparisons as in ( B ).

    Article Snippet: PE-conjugated anti-mouse CD4, FITC-conjugated anti-mouse CD8, and PE-Cy5-conjugated anti-mouse CD3ε monoclonal antibodies were obtained from Lianke Bio (Hangzhou, China).

    Techniques: Enzyme-linked Immunosorbent Assay, Control, Microneutralization Assay