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antipsd95 monoclonal mouse  (Thermo Fisher)


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    Structured Review

    Thermo Fisher antipsd95 monoclonal mouse
    Antipsd95 Monoclonal Mouse, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antipsd95/pm36736611-70-9-13
    Average 86 stars, based on 1 article reviews
    antipsd95 monoclonal mouse - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Membrane remodeling by FAM92A1 during brain development regulates neuronal morphology, synaptic function, and cognition.
    Article Snippet: After drying, the sampleswere visualizedusing transmission electron microscopy (FEI, Tacnai, G2 spirit, Hillsboro, Oregon) operating at 100 kV, and images were captured using a CCD camera (MoradaG3; EMSIS) with RADIUS software at RT. .. Primary antibodies used for immunofluorescence and ImmunoEM are listed as follows: rabbit anti-FAM92A1 (1:100, Cat# HPA034760, Sigma), rabbit anti-FAM92A1 (1:100, Cat# 24803-1-AP, Proteintech), rabbit anti-TOM20 (1:1000, Cat# 11802-1-AP, Proteintech), mouse anti- Nature Communications | (2024) 15:6209 20 VDAC (1:50, Cat# ab14734, Abcam), mouse anti-Clathrin (1:50, Cat# 610449, BD Bioscience), mouse anti-Caveolin (1:50, Cat# 610406, BD Bioscience), mouse anti-PSD95 (1:500, Cat# MA1-046, Invitrogen), mouse anti-SV2 (1:250, Cat# SV2, DSHB), mouse anti-MAP2 (1:400, Cat# 13-1500, Invitrogen), rabbit anti-vGlut1 (1:1000, Cat# 55491-1-AP, Proteintech), rabbit anti-Vimentin (1:500, Cat# 10366-1-AP, Proteintech). .. Actin was visualized with Alexa Fluor 647 conjugated to phalloidin (1:200; Cat# A22287, Invitrogen).

    Article Title: Membrane remodeling by FAM92A1 during brain development regulates neuronal morphology, synaptic function, and cognition
    Article Snippet: The immunolabeled samples were placed on drops of uranyl acetate/methyl cellulose (1:9) for 5 min. After drying, the samples were visualized using transmission electron microscopy (FEI, Tacnai, G2 spirit, Hillsboro, Oregon) operating at 100 kV, and images were captured using a CCD camera (MoradaG3; EMSIS) with RADIUS software at RT. .. Primary antibodies used for immunofluorescence and Immuno-EM are listed as follows: rabbit anti-FAM92A1 (1:100, Cat# HPA034760, Sigma), rabbit anti-FAM92A1 (1:100, Cat# 24803-1-AP, Proteintech), rabbit anti-TOM20 (1:1000, Cat# 11802-1-AP, Proteintech), mouse anti-VDAC (1:50, Cat# ab14734, Abcam), mouse anti-Clathrin (1:50, Cat# 610449, BD Bioscience), mouse anti-Caveolin (1:50, Cat# 610406, BD Bioscience), mouse anti-PSD95 (1:500, Cat# MA1-046, Invitrogen), mouse anti-SV2 (1:250, Cat# SV2, DSHB), mouse anti-MAP2 (1:400, Cat# 13-1500, Invitrogen), rabbit anti-vGlut1 (1:1000, Cat# 55491-1-AP, Proteintech), rabbit anti-Vimentin (1:500, Cat# 10366-1-AP, Proteintech). .. Actin was visualized with Alexa Fluor 647 conjugated to phalloidin (1:200; Cat# A22287, Invitrogen).

    other:

    Article Title: Clec7a Worsens Long-Term Outcomes after Ischemic Stroke by Aggravating Microglia-Mediated Synapse Elimination.
    Article Snippet: After blocking, sections were rinsed and incubated with the following primary antibodies in dilution buffer overnight at 4 °C: anti-Iba-1 (1:500, 019–19741, WAKO); antiCD68 (1:500, 14-0681-82, Invitrogen); anti-PSD95 (1:100, MA1-046, Invitrogen); anti-Synaptophysin (1:100, S5768, Sigma); anti-Clec7a (1:100, mabg-mdct, Invivogen); anti-MD2 (1:100, GTX85517, GTX85121, GeneTex), anti-C1q (1:100, ab11861, abcam), anti-C3 (1:100, ab11862, abcam), anti-Lgals9 (1:100, abs143512, Absin).

    Article Title: FNDC5/Irisin mitigates high glucose-induced neurotoxicity in HT22 cell via ferroptosis.
    Article Snippet: ACSL4 antibody (Santa Cruz Biotechnology, #sc-365230, diluted at 1:100), postsynaptic density protein 95 (PSD95) antibody (Invitrogen, #MA1-046, diluted at 1:500), FNDC5 antibody (Abcam, #ab174833, diluted at 1:1,000), β-actin antibody (Abcam, #ab115777, diluted at 1:1,000), were all shaken overnight and incubated.

    Article Title: Apolipoprotein E ε4 exacerbates microglia-mediated complement-dependent synapse loss caused by neuronal Tpk deficiency
    Article Snippet: The primary antibodies used were as follows: mouse anti-Synaptophysin (1:1000, MAB5258A4, Merck), mouse anti-PSD 95 (1:1000, MA1-046, Invitrogen), rabbit anti-C3aR (1:1000, sc-133172, Santa Cruz), mouse anti-Apolipoprotein E4 (1:1000, M067-3, MBL Life Science), rabbit anti-TPK1 (1:1000, ab230263, Abcam), rabbit anti-APP (1:1000, ab32136, Abcam), rabbit anti-BACE1 (1:1000, 5606, CST), mouse anti-Tau5 (1:1000, AHB0042, Invitrogen), rabbit anti-pTau (Thr181) (1:1000, 12885, CST), rabbit anti-pTau (Ser396) (1:1000, 9632, CST), mouse anti-pTau (Ser202, Thr205) (1:1000, MN1020, Invitrogen), rabbit anti-β-actin (1:2000, 4970, CST), rabbit anti-tubulin (1:2000, 2146, CST).

    Article Title: FcγR- and CD9-dependent synapse engulfing microglia in the thalamus drives cognitive impairment following cortical brain injury
    Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-Iba1 (1:500; WAKO, #019–19741,), Alexa Fluor 488-conjugated rabbit monoclonal anti-Iba1 (1:500; abcam, #ab225260), mouse monoclonal anti-Iba1 (1:500; Sigma-Aldrich, #MABN92), Alexa Fluor 647-conjugated mouse monoclonal anti-NeuN (1:500; abcam, #ab190565), rabbit monoclonal anti-NeuN (1:500; abcam, #ab177487), rabbit monoclonal anti-c-Fos (1:500; Cell Signaling Technology, #2250), rabbit monoclonal anti-TSPO (1:100; abcam, #ab109497), rat monoclonal anti-CD68 (1:2,000; Novus Biologicals, #NBP2-33337), mouse monoclonal anti-TNF-α (1:100; abcam, #ab1793), rat monoclonal anti-RFP (1:500; Chromotek, #5F8), rabbit monoclonal anti-HA-tag (1:500; Cell Signaling Technology, #3724), armenian hamster monoclonal anti-CD31/PECAM1 (1:100; DSHB, #2H8-s), rabbit polyclonal CD9 (1:100; Proteintech, #20597-1-AP), CD9 (1:100; BD Biosciences, #553758), rabbit polyclonal anti-FcγRIII (1:100; abcam, #ab203883), rabbit polyclonal anti-p-Syk (1:200; Cell Signaling, #2710T), mouse monoclonal anti-PSD95 (1:500; Thermo Fisher Scientific, #MA1-046), goat polyclonal anti-LPL (1:100; R&D Systems, #AF7197), chicken monoclonal anti-Tmem119 (1:500; Synaptic Systems, #400009), sheep polyclonal anti-Trem2 (1:100; R & D, #AF1729), rabbit polyclonal anti-Claudin5 (1:100, Thermo Fisher Scientific, #34-1600), and rabbit polyclonal anti-Ifitm3 (1:100; Proteintech, #11714-1-AP).

    Staining:

    Article Title: Tau association with synaptic mitochondria coincides with energetic dysfunction and excitatory synapse loss in the P301S tauopathy mouse model.
    Article Snippet: .. In brief, 50-μm thick coronal brain sections were collected using a vibrating microtome and subsequently stained with the following primary antibodies after Mouse-On-Mouse (M.O.M.) blocking treatment: Total Tau (TauD) (1:600, Cat #46687, Cell Signaling Technology, Danvers, MA, USA), PSD95 (1:1000, Cat #MA1–046 Invitrogen, Waltham, MA, USA), and synaptophysin (1:400, Cat #101006, Synaptic Systems, Göttingen, Germany). .. For secondary antibody conjugation, the following antibodies were used: Alexa Goat anti-Chicken 568 (1:1000, Cat #A11041, Invitrogen), Alexa Donkey anti-Mouse 647 (1:1000, Cat #A-31571, Invitrogen), Alexa Goat anti-Rabbit 488 1:1000, Cat #A11008, Invitrogen), and DAPI for nuclear counterstain.

    Blocking Assay:

    Article Title: Tau association with synaptic mitochondria coincides with energetic dysfunction and excitatory synapse loss in the P301S tauopathy mouse model.
    Article Snippet: .. In brief, 50-μm thick coronal brain sections were collected using a vibrating microtome and subsequently stained with the following primary antibodies after Mouse-On-Mouse (M.O.M.) blocking treatment: Total Tau (TauD) (1:600, Cat #46687, Cell Signaling Technology, Danvers, MA, USA), PSD95 (1:1000, Cat #MA1–046 Invitrogen, Waltham, MA, USA), and synaptophysin (1:400, Cat #101006, Synaptic Systems, Göttingen, Germany). .. For secondary antibody conjugation, the following antibodies were used: Alexa Goat anti-Chicken 568 (1:1000, Cat #A11041, Invitrogen), Alexa Donkey anti-Mouse 647 (1:1000, Cat #A-31571, Invitrogen), Alexa Goat anti-Rabbit 488 1:1000, Cat #A11008, Invitrogen), and DAPI for nuclear counterstain.



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