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permafluor mounting solution containing 4’,6-diamidino-2-phenylindole (dapi)  (Dojindo Labs)


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    Dojindo Labs permafluor mounting solution containing 4’,6-diamidino-2-phenylindole (dapi)
    Permafluor Mounting Solution Containing 4’,6 Diamidino 2 Phenylindole (Dapi), supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mounting+solution+containing+dapi/dapi+solution+d532/pmc11704040-88-8-11
    Average 90 stars, based on 1 article reviews
    permafluor mounting solution containing 4’,6-diamidino-2-phenylindole (dapi) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Methylcobalamin-containing nanofiber sheets have better neuroprotective effects than small intestinal submucosa sheets
    Article Snippet: Samples were incubated at 4 °C overnight using primary antibodies to neurofilament 200 (NF200) (1:1,000; N4142; Sigma-Aldrich, St Louis, MO, USA) and CD68 (1:200; ab31630; Abcam, Boston, MA, USA) or myelin basic protein (MBP) (1:1,000; NE1018; Calbiochem, San Diego, CA, USA) and then incubated at 20–25 °C for 1 h using the following secondary antibodies: Alexa Fluor 488 goat anti-rabbit immunoglobulin G (IgG) and Alexa Fluor 568 goat anti-mouse IgG (1:1,000; Life Technologies, Carlsbad, CA, USA). .. Nuclei were stained with Permafluor mounting solution containing 4’,6-diamidino-2-phenylindole (DAPI) (D532; DOJINDO, Kumamoto, Japan). .. For each nerve, the number of intraneural DAPI + or CD68 + cells, number of axons, distribution of axon diameters, and myelination ratio (MBP + axon number/total axon number) were calculated using the ImageJ software (version 1.54f).

    Article Title: Electrospun nanofiber sheets incorporating methylcobalamin promote nerve regeneration and functional recovery in a rat sciatic nerve crush injury model.
    Article Snippet: Accepted Manuscript Electrospun nanofiber sheets incorporating methylcobalamin promote nerve regeneration and functional recovery in a rat sciatic nerve crush injury model Koji Suzuki, Hiroyuki Tanaka, Mitsuhiro Ebara, Koichiro Uto, Hozo Matsuoka, Shunsuke Nishimoto, Kiyoshi Okada, Tsuyoshi Murase, Hideki Yoshikawa PII: S1742-7061(17)30099-5 DOI: http://dx.doi.org/10.1016/j.actbio.2017.02.004 Reference: ACTBIO 4725 To appear in: Acta Biomaterialia Received Date: 5 October 2016 Revised Date: 23 January 2017 Accepted Date: 2 February 2017 Please cite this article as: Suzuki, K., Tanaka, H., Ebara, M., Uto, K., Matsuoka, H., Nishimoto, S., Okada, K., Murase, T., Yoshikawa, H., Electrospun nanofiber sheets incorporating methylcobalamin promote nerve regeneration and functional recovery in a rat sciatic nerve crush injury model, Acta Biomaterialia (2017), doi: http:// dx.doi.org/10.1016/j.actbio.2017.02.004 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Article Title: Administration of Oxygen Ultra-Fine Bubbles Improves Nerve Dysfunction in a Rat Sciatic Nerve Crush Injury Model
    Article Snippet: After blocking with PBS containing 0.2% Triton X and 5% BSA, the samples were incubated with primary antibodies against neuronal class III β-tubulin (Tuj1) mouse monoclonal antibody (1:1000; MMS-435P; Covance, Dedham, MA, USA) overnight at 4 °C, followed by incubation with the appropriate secondary antibodies including Alexa Fluor 488 goat anti-mouse IgG (1:1000; A-11029; Molecular Probes). .. The nuclei were stained with the Permafluor mounting solution containing DAPI (D532; DOJINDO, Kumamoto, Japan). ..

    Article Title: Neurotropin® accelerates peripheral nerve regeneration in a rat sciatic nerve crush injury model.
    Article Snippet: .. The nuclei were stained with PermaFluor AqueousMountingMedium containing DAPI (D532; DOJINDO). .. The number of total axons and myelinated axons were calculated from three random microscopic fields of each sample at 200 magnification using the NIS Elements BR software (Nikon, Tokyo, Japan).

    other:

    Article Title: SARS-CoV-2 propagation to the TPH2-positive neurons in the ventral tegmental area induces cell death via GSK3β-dependent accumulation of phosphorylated tau.
    Article Snippet: TUNEL staining was performed using the TUNEL assay kit (#25879, CST) according to the manufacturer’s instruction, followed by staining with DAPI (D532, Dojindo).

    Article Title: SARS-CoV-2 propagation to the TPH2-positive neurons in the ventral tegmental area induces cell death via GSK3β-dependent accumulation of phosphorylated tau.
    Article Snippet: After washing with PBS three times for 5 min each, secondary fluorescence-labeled antibodies (anti-rabbit IgG Alexa fluor 488: 4412S, anti-mouse IgG Alexa fluor 594: 8890S, cell signaling technology, USA) were added at a 1:500 dilution and DAPI (D532, Dojindo) at a 1:1000 dilution in antibody diluent with background reducing components (Dako, CA, USA) and incubated for 1 h at RT.



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