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nebnext quick ligation module  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext quick ligation module
    Nebnext Quick Ligation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1087 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/module/NEBNext+Quick+Ligation+Module/pm42012196-218-76-76
    Average 99 stars, based on 1087 article reviews
    nebnext quick ligation module - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Impact of extended fertilization on soil microbial diversity: a comparative full-length 16S rRNA amplicon analysis.
    Article Snippet: For end preparation and dA tailing, 200 ng of DNA was used, followed by barcode ligation with the Native Barcoding Kit 96 V14 (SQK-NBD114.96; Oxford Nanopore). .. Sequencing adaptors were ligated using the NEBNext Quick Ligation Module (NEB, E6056), and the resulting DNA libraries were pooled [56]. .. The fully adapterligated and purified library was combined with 37.5 μL of sequencing buffer and 25.5 μL of loading beads, then loaded onto the R10 flow cell (FLO-MIN114; Oxford Nanopore Technologies) for sequencing on the MinION Mk1C.

    Article Title: Occurrence and subtyping of the intestinal protozoan Blastocystis sp. in French oyster areas reveal potential fecal contamination routes and associated risks to consumer health
    Article Snippet: Briefly, sequencing libraries were prepared with the Native Barcoding Kit (Oxford Nanopore Technologies) following the manufacturer's instructions. .. Amplicons were end-repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Ipswich, MA, USA), barcoded with the Blunt/TA Ligase Master Mix (NEB), and ligated to sequencing adapters using the NEBNext Quick Ligation Module (NEB). ..

    Ligation:

    Article Title: Impact of extended fertilization on soil microbial diversity: a comparative full-length 16S rRNA amplicon analysis.
    Article Snippet: For end preparation and dA tailing, 200 ng of DNA was used, followed by barcode ligation with the Native Barcoding Kit 96 V14 (SQK-NBD114.96; Oxford Nanopore). .. Sequencing adaptors were ligated using the NEBNext Quick Ligation Module (NEB, E6056), and the resulting DNA libraries were pooled [56]. .. The fully adapterligated and purified library was combined with 37.5 μL of sequencing buffer and 25.5 μL of loading beads, then loaded onto the R10 flow cell (FLO-MIN114; Oxford Nanopore Technologies) for sequencing on the MinION Mk1C.

    Article Title: Long-read RNA sequencing identifies non-coding isoform switching as a regulator of cell fate
    Article Snippet: The blunt DNA ends were prepared using the NEBNext UltraTM II End Repair/dA-Tailing module (NEB #E7546), adding a 3’ dA tail and phosphorylating the 5’ end. .. Barcodes were ligated to the DNA fragments with a Blunt/TA ligase (NEB #M0367), barcoded samples were pooled and motor protein adapters were finally ligated with the NEBNext Quick Ligation Module (NEB #E6056). ..

    Article Title: Genomic epidemiology reveals multiple introductions and local transmission of SARS-CoV-2 in Sudan from 2020 to 2022
    Article Snippet: Barcode ligation was done using the native barcoding expansion (EXP-NBD196) kit (Oxford Nanopore Technology, UK) and NEBNext Blunt/TA Ligase Master Mix (NEB, US). .. Thereafter, adaptor ligation was done using NEBNext Quick Ligation Module reagents (NEB, US), Adaptor Mix II (ONT, UK) and 100ng of the barcoded sample following the manufacturers instruction. .. The final loading concentration was normalised to 15ng and loaded on a SpotON R9.4.1 flow cell, which was sequenced on a GridION device [33].

    Article Title: Occurrence and subtyping of the intestinal protozoan Blastocystis sp. in French oyster areas reveal potential fecal contamination routes and associated risks to consumer health
    Article Snippet: Briefly, sequencing libraries were prepared with the Native Barcoding Kit (Oxford Nanopore Technologies) following the manufacturer's instructions. .. Amplicons were end-repaired and dA-tailed using the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Ipswich, MA, USA), barcoded with the Blunt/TA Ligase Master Mix (NEB), and ligated to sequencing adapters using the NEBNext Quick Ligation Module (NEB). ..

    Article Title: Loss of RafE impairs raffinose utilization and increases early pneumococcal burden in mice.
    Article Snippet: This was followed by the ligation of Oxford Nanopore-specific native barcodes onto each sample using the Native Barcoding Kit 24 V14 (SQK-NBD114.24 Oxford Nanopore) and the third-party reagent NEB Blunt/TA Ligase Master Mix (NEB). .. Barcoded samples were pooled, and the Native Adaptor (Oxford Nanopore), used to ratchet DNA strains through the nanopores, was ligated to each DNA fragment using NEBNext Quick Ligation Reaction Module (NEB). .. The library was enriched for fragments 3 kb or longer by the long fragment buffer (SQK-NBD114.24 Oxford Nanopore).

    Article Title: Nested mobile genetic elements mediating antimicrobial resistance genes mobility within and between Acinetobacter isolates
    Article Snippet: NEBNext® Ultra II End repair/dA-tailing Module , New England BioLabs , E7546S. .. NEBNext® Quick Ligation Module , New England BioLabs , E6056S. .. Oxford Nanopore Technologies Ligation Sequencing Kit , Oxford Nanopore Technologies , SQK-LSK109.

    other:

    Article Title: Protocol for rolling circle amplification-based nanopore sequencing of eccDNA from cell and plasma samples.
    Article Snippet: Biological samples Pooled human plasma sample used for cfDNA extraction This paper N/A Chemicals, peptides, and recombinant proteins FastDigest MssI enzyme Thermo Cat# FD1344 Exonuclease V NEB Cat# M0345 Adenosine 5′-Triphosphate (ATP) (10 mM) NEB Cat# P0756 Nuclease-free water (NFW) Solarbio Cat# R1600 DNA extraction solution (phenol-chloroformisoamyl alcohol, 25:24:1) Solabio Cat# P1012 Glycogen Roche Cat# 10901393001 3 M sodium acetate Solabio Cat# A1070 TE Buffer Solarbio Cat# T1120 Phi29 MAX DNA Polymerase Vazyme Cat# EP0091 dNTPs (10 mM) NEB Cat# N0447 Recombinant Albumin (20mg/mL) NEB Cat# B9200 Pyrophosphatase, Inorganic Thermo Cat# EF0221 T7 Endonuclease I NEB Cat# M0302 T4 Ligase NEB Cat# E6056 Critical commercial assays 23 ChamQ Universal SYBR qPCR Master Mix Vazyme Cat# Q711 E.Z.N.A. Plasmid DNA Mini Kit I Omega Cat# D6943 Plasma Cell-Free DNA Extraction Kit APOSTLE Cat# A17622CN Qubit 13 dsDNA HS Assay Kits Thermo Fisher Scientific Cat# Q33231 SPRI beads Beckman Cat# B23317 NEBNext FFPE DNA Repair Mix NEB Cat# E7360 Ultra II End-prep enzyme mix NEB Cat# E7546 NEB Blunt/TA Ligase Master Mix NEB Cat# M0367 Native Barcoding Kit 24 V14 ONT Cat# SQK-NBD114.24 Flow Cell Wash Kit ONT Cat# EXP-WSH004 Deposited data Raw nanopore sequencing data for eccDNA profiling of Li et al.1 This paper PRJCA052047 Raw nanopore sequencing data for eccDNA profiling of four Huh7 cell samples and one pooled human plasma sample This paper PRJCA069021 Experimental models: Cell lines Human: HepG2 Wuhan Procell Biotechnology Co., Ltd. Cat# CL-0103 Human: Huh7 Shanghai Xunyi Biotechnology Co., Ltd. Cat# MXC58 Oligonucleotides Exo-random primer (500 μM) Thermo Cat# SO181 Primer ND1 Forward: CCCTAAAACCCGCCACATCT Reverse: GAGCGATGGTGAGAGCTAAGGT This paper N/A Primer H-GAPDH Forward: CTGGGCTACACTGAGCACC Reverse: AAGTGGTCGTTGAGGGCAATG This paper N/A Primer MIR621-A Forward: GGATCATATTCCACGCTGACTAT Reverse: AGTCCCATTTCTATTTTACTTCG This paper N/A Primer MIR621-B Forward: AATTCACTGACCAACCAACCAA Reverse: TTTGGTCAGTGAATACATACATATGGC This paper N/A (Continued on next page) STAR Protocols 7, 104865, December 18, 2026 3 STEP-BY-STEP METHOD DETAILS Isolate crude circular DNA Timing: 3 h In this step, we describe the crude extraction of circular DNA from tumor cell lines and circular cfDNA from plasma samples.



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    Image Search Results


    Journal: iScience

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

    doi: 10.1016/j.isci.2026.117639

    Figure Lengend Snippet:

    Article Snippet: Libraries were prepared by blunting and dA-tailing the amplicons using the End Prep library kit (NEB; Ref: E7546) followed by NEBNext Ultra II Ligation Module kit (NEB, Ref: E7595) with the adapters obtained from NEBNext Multiplex Oligos for Illumina (NEB, Ref:E7600) following standard protocols.

    Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software

    Journal: iScience

    Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

    doi: 10.1016/j.isci.2026.117639

    Figure Lengend Snippet:

    Article Snippet: End Prep library kit , NEB , E7546.

    Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software

    Journal: iScience

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12

    doi: 10.1016/j.isci.2026.117719

    Figure Lengend Snippet:

    Article Snippet: Clampfit software , Molecular Devices , RRID: SCR_011323.

    Techniques: Cell-Signaling, Virus, Recombinant, Clinical Proteomics, Membrane, Staining, Cloning, Software