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hotstart polymerase modification kit  (Biotium)


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    Structured Review

    Biotium hotstart polymerase modification kit
    Hotstart Polymerase Modification Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modifications/HotStart+Enzyme+Modification+Kit/us09682970-470-73-77
    Average 90 stars, based on 1 article reviews
    hotstart polymerase modification kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Fluorescent compounds and uses thereof
    Article Snippet: The data in FIG. 9 were collected under the following conditions: mRNA was purified from 4×106 Jurkat cells using Qiagen's RNeasy Kit and cDNA was then generated using oligo-dT primers and the ProtoScript First Strand cDNA Synthesis Kit (New England BioLabs). .. Serial 1:2 dilutions of the cDNA in water were prepared and added to PCR reactions consisting of a final concentration of 25 mM Tris-HCl (pH 8.6), 5 mM KCl, 10 mM (NH4)2SO4, 2 mM MgCl2, 2.5% glycerol, 5% DMSO, 200 uM dNTPs (New England BioLabs), 0.01% Tween-20, non-acetylated BSA (Sigma), 500 nM GAPDH Forward primer (5′-GAAGGTGAAGGTCGGAGTC-3′), 500 nM GAPDH Reverse primer (5′-GAAGATGGTGATGGGATTTC-3′) (Integrated DNA Technologies), 1 U Taq polymerase (Syzygy) modified using a HotStart Polymerase Modification Kit (Biotium, Inc.) and 0.34 uM compound 7. ..

    Concentration Assay:

    Article Title: Fluorescent compounds and uses thereof
    Article Snippet: The data in FIG. 9 were collected under the following conditions: mRNA was purified from 4×106 Jurkat cells using Qiagen's RNeasy Kit and cDNA was then generated using oligo-dT primers and the ProtoScript First Strand cDNA Synthesis Kit (New England BioLabs). .. Serial 1:2 dilutions of the cDNA in water were prepared and added to PCR reactions consisting of a final concentration of 25 mM Tris-HCl (pH 8.6), 5 mM KCl, 10 mM (NH4)2SO4, 2 mM MgCl2, 2.5% glycerol, 5% DMSO, 200 uM dNTPs (New England BioLabs), 0.01% Tween-20, non-acetylated BSA (Sigma), 500 nM GAPDH Forward primer (5′-GAAGGTGAAGGTCGGAGTC-3′), 500 nM GAPDH Reverse primer (5′-GAAGATGGTGATGGGATTTC-3′) (Integrated DNA Technologies), 1 U Taq polymerase (Syzygy) modified using a HotStart Polymerase Modification Kit (Biotium, Inc.) and 0.34 uM compound 7. ..

    Modification:

    Article Title: Fluorescent compounds and uses thereof
    Article Snippet: The data in FIG. 9 were collected under the following conditions: mRNA was purified from 4×106 Jurkat cells using Qiagen's RNeasy Kit and cDNA was then generated using oligo-dT primers and the ProtoScript First Strand cDNA Synthesis Kit (New England BioLabs). .. Serial 1:2 dilutions of the cDNA in water were prepared and added to PCR reactions consisting of a final concentration of 25 mM Tris-HCl (pH 8.6), 5 mM KCl, 10 mM (NH4)2SO4, 2 mM MgCl2, 2.5% glycerol, 5% DMSO, 200 uM dNTPs (New England BioLabs), 0.01% Tween-20, non-acetylated BSA (Sigma), 500 nM GAPDH Forward primer (5′-GAAGGTGAAGGTCGGAGTC-3′), 500 nM GAPDH Reverse primer (5′-GAAGATGGTGATGGGATTTC-3′) (Integrated DNA Technologies), 1 U Taq polymerase (Syzygy) modified using a HotStart Polymerase Modification Kit (Biotium, Inc.) and 0.34 uM compound 7. ..



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