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AllCells LLC human monocyte-derived dendritic cells (modcs)
Human Monocyte Derived Dendritic Cells (Modcs), supplied by AllCells LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modcs/monocyte+derived+dendritic+cells/us12054507-792-0-10
Average 90 stars, based on 1 article reviews

human monocyte-derived dendritic cells (modcs) - by Bioz Stars, 2026-10

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Article Title: Antiviral compounds
Article Snippet: Human monocyte-derived dendritic cells (moDCs) were derived from CD14+ monocytes (AllCells) cultured in Human Mo-DC Differentiation medium containing GM-CSF and IL-4 (Miltenyi Biotec). .. Human monocyte-derived dendritic cells (moDCs) were derived from CD14+ monocytes (AllCells) cultured in Human Mo-DC Differentiation medium containing GM-CSF and IL-4 (Miltenyi Biotec). .. On day 7, moDCs were harvested by mechanical disruption, washed and suspended in serum-free RPMI. moDCs were infected with Vero-derived Dengue 2, New Guinea strain (NGC) at a MOI=0.1 for two hours in serum-free RPMI with gentle agitation at 37° C. Cells were washed and resuspended in 10% serum-containing RPMI (Gibco, supplemented with sodium pyruvate, NEAA, Penicillin-Streptomycin).

Article Title: Antiviral compounds
Article Snippet: .. Human monocyte-derived dendritic cells (moDCs) were derived from CD14+ monocytes (AllCells) cultured in Human Mo-DC Differentiation medium containing GM-CSF and IL-4 (Miltenyi Biotec). .. On day 7, moDCs were harvested by mechanical disruption, washed and suspended in serum-free RPMI. moDCs were infected with Vero-derived Dengue 2, New Guinea strain (NGC) at a MOI=0.1 for two hours in serum-free RPMI with gentle agitation at 37° C. Cells were washed and resuspended in 10% serum-containing RPMI (Gibco, supplemented with sodium pyruvate, NEAA, Penicillin-Streptomycin).

Article Title: Compositions comprising circular polyribonucleotides and uses thereof
Article Snippet: Transfection of the circular RNA include the following conditions: (1) naked circular RNA in cell culture media (Lingor et al 2004); (2) electroporation (Muller et al 2015); (3) cationic lipids (SNALP, Vaxfectin) (Chesnoy and Huang, 2000); (3) cationic polymers (PEI, polybrene, DEAE-dextran) (Turbofect); (4) virus-like particles (L1 from HPV, VP1 from polyomavirus) (Tonges et al 2006); (5) exosomes (Exo-Fect from SBI); (6) nanostructured calcium phosphate (nanoCaP)(Olton et al 2006); (6) peptide transduction domains (TAT, polyR, SP, pVEC, SynB1, etc) (Zhang et al 2009); (7) vesicles (VSV-G, TAMEL) (Liu et al 2017); (8) cell squeezing; (SQZ Biotechnologies) (9) nanoparticles (Neuhaus et al 2016); and/or (10) magnetofection (Mair et al 2009). .. In addition, (1) primary murine dendritic cells; (2) Human embryonic kidney 293 cells stabile expressing TLR-7, 8 or 9 (InvivoGen); (3) monocyte derived dendritic cells (AllCells) or (4) Raw 264.7 cells are transfected with a DNA plasmid including ZKSCAN1 or td introns that produce a circular RNA encoding GFP as described above. .. For qRT-PCR analysis, total RNA is isolated from cells using a phenol based RNA isolation solution (TRIzol) and an RNA isolation kit (QIAGEN) following the manufacturer's instructions. qRT-PCR analysis is performed in triplicate using a PCR master mix (Brilliant II SYBR Green qRT-PCR Master Mix) and a PCR cycler (LightCycler 480). mRNA levels for well-known innate immunity regulators such as RIG-I, MDA5, OAS, OASL, and PKR are quantified and normalized to actin, GAPDH, or HPRT values.

Cell Culture:

other:

Article Title: Small molecule AZD4635 inhibitor of A 2A R signaling rescues immune cell function including CD103 + dendritic cells enhancing anti-tumor immunity.
Article Snippet: Raw counts were normalized using nSolver V.4.0 software (Nanostring Technologies).

Article Title: Preclinical and clinical characterization of the RORγt inhibitor JNJ-61803534
Article Snippet: Frozen purified human CD4 + CD25 + natural Treg cells (nTreg), monocyte-derived dendritic cells (DC) and CD4 + CD25 - T effector cells (Teff) (Allcells, LLC, Alameda, CA) were thawed and co-cultured in the presence or absence of JNJ-61803534.

Article Title: Small molecule AZD4635 inhibitor of A 2A R signaling rescues immune cell function including CD103 + dendritic cells enhancing anti-tumor immunity
Article Snippet: Isolated CD103 + DC were co-cultured at a 1:20 ratio with CFSE-labeled (CellTrace CFSE ThermoFisher Scientific cat# C34534), negatively selected CD8 + T cells (Miltenyi Mouse CD8 + T cell Isolation Kit, cat# 130-104-075) in 200 μL T cell complete medium for 4 days with subsequent proliferation evaluated by dye dilution analysis using FlowJo V.10 software following acquisition on a MACSQuant-10 flow cytometer (Miltenyi Biotec).

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Depletion of DCs and induction of tolerogenic DC by ADCC suppress T-cell activation. (A) Study design of mixed lymphocyte reaction (MLR) assay after using anti-DCIR antibody to deplete monocyte-derived DCs differentiated and matured in vitro. (B) Gating strategy of flow cytometry analysis on the activation of allogenic T cells. (C–F) T-cell proliferation and activation indicated by the increased T-cell count and CD69 expression on CD4 + (C, D) and CD8 + T cells (E, F). (G–K) Cytokine production from the MLR assay as described in (A). Representative data from 1 of 2 donors are shown, with error bars indicating mean ± SEM.

Journal: ImmunoHorizons

Article Title: Direct contact with natural killer cells reprograms monocyte-derived dendritic cells into a tolerogenic phenotype

doi: 10.1093/immhor/vlag007

Figure Lengend Snippet: Depletion of DCs and induction of tolerogenic DC by ADCC suppress T-cell activation. (A) Study design of mixed lymphocyte reaction (MLR) assay after using anti-DCIR antibody to deplete monocyte-derived DCs differentiated and matured in vitro. (B) Gating strategy of flow cytometry analysis on the activation of allogenic T cells. (C–F) T-cell proliferation and activation indicated by the increased T-cell count and CD69 expression on CD4 + (C, D) and CD8 + T cells (E, F). (G–K) Cytokine production from the MLR assay as described in (A). Representative data from 1 of 2 donors are shown, with error bars indicating mean ± SEM.

Article Snippet: For inhibiting the IDO1 activity, indoximod (MCE, Cat#HY-16724) was added together with GM-CSF and IL-4 during MoDC differentiation and MoDC maturation induced by 1 μg/mL CpG ODN2006 (Invivogen, Cat#tlrl-2006) and 500 ng/mL CD40L (PeproTech, Cat#310-02).

Techniques: Activation Assay, Mlr Assay, Derivative Assay, In Vitro, Flow Cytometry, Analysis, Cell Characterization, Expressing, FACS

IL-10 signaling is critical for the tolerogenic effect from the NK-programmed DC. (A) Study design of knocking-out transcription factors and evaluating the resultant DC tolerance effects. (B) IL-10, TNF-α, and IL-6 cytokine production was assayed from LPS-stimulated MoDCs after CRISPR-Cas9 editing. CRISPR-KO MoDC effect on T-cell activation in MLR evaluated by flow cytometry showing (C) CD25 expression on CD4 T cells and CD8 T cells ( n = 3 donors) and (D) evaluated MLR secreted cytokines IL-10, TNF-α, and IFN-γ ( n = 3 donors). Means ± SEM are shown, and statistical analysis is determined by one-way ANOVA Fisher least significant difference test compared to the scramble sgRNA control. * P < .05, ** P < .01, *** P < .001, **** P < .0001. (E) Study design of neutralizing IL-10 during the depletion of MoDCs by anti-DCIR–induced ADCC effect. (F) Cell count and (G, H) MFI of DC co-stimulatory/maturation markers (CD40 and CD44) on surviving CD14 + CD11c + MoDCs in the ADCC assay induced by anti-DCIR antibody in combination with or without anti-IL-10 neutralizing antibody ( n = 3 donors). (I) IL-10R expression and (J) cell viability of the MoDCs differentiated by GM-CSF and IL-4 treatment and matured by CpG and CD40L stimulation, along with indoximod, an IDO1 inhibitor ( n = 4 donors). (K) Schematic summary of the tolerogenesis of DCs from the NK engagement induced by DC-targeting antibody (eg anti-DCIR).

Journal: ImmunoHorizons

Article Title: Direct contact with natural killer cells reprograms monocyte-derived dendritic cells into a tolerogenic phenotype

doi: 10.1093/immhor/vlag007

Figure Lengend Snippet: IL-10 signaling is critical for the tolerogenic effect from the NK-programmed DC. (A) Study design of knocking-out transcription factors and evaluating the resultant DC tolerance effects. (B) IL-10, TNF-α, and IL-6 cytokine production was assayed from LPS-stimulated MoDCs after CRISPR-Cas9 editing. CRISPR-KO MoDC effect on T-cell activation in MLR evaluated by flow cytometry showing (C) CD25 expression on CD4 T cells and CD8 T cells ( n = 3 donors) and (D) evaluated MLR secreted cytokines IL-10, TNF-α, and IFN-γ ( n = 3 donors). Means ± SEM are shown, and statistical analysis is determined by one-way ANOVA Fisher least significant difference test compared to the scramble sgRNA control. * P < .05, ** P < .01, *** P < .001, **** P < .0001. (E) Study design of neutralizing IL-10 during the depletion of MoDCs by anti-DCIR–induced ADCC effect. (F) Cell count and (G, H) MFI of DC co-stimulatory/maturation markers (CD40 and CD44) on surviving CD14 + CD11c + MoDCs in the ADCC assay induced by anti-DCIR antibody in combination with or without anti-IL-10 neutralizing antibody ( n = 3 donors). (I) IL-10R expression and (J) cell viability of the MoDCs differentiated by GM-CSF and IL-4 treatment and matured by CpG and CD40L stimulation, along with indoximod, an IDO1 inhibitor ( n = 4 donors). (K) Schematic summary of the tolerogenesis of DCs from the NK engagement induced by DC-targeting antibody (eg anti-DCIR).

Techniques: CRISPR, Activation Assay, Flow Cytometry, Expressing, Analysis, Control, Cell Characterization, ADCC Assay, Immunopeptidomics, Immune Cell, FACS

Surviving monocyte-derived DCs following ADCC exhibit tolerogenic phenotypes. (A) Flow cytometry analysis of house-made anti-DCIR (clone 5E11) antibody binding to the CD14 + monocytes, B cells, T cells, and NK cells from human PBMCs ( n = 4 donors). Means ± SEM are shown, and statistical analysis is determined by one-way ANOVA test with Dunnett correction compared to the isotype staining control. ** P < .01. (B) Flow cytometry analysis of our anti-DCIR (5E11) antibody’s binding to the CD14 + monocytes, monocyte-derived DCs and macrophages ( n = 4 donors). (C) Study design of ADCC assay using anti-DCIR antibody to deplete monocyte-derived DCs differentiated and matured in vitro. (D) Cell count, (E–G) Mean Fluorescence Intensity (MFI) of surface co-stimulatory markers (CD11c, CD40, and CD44), and (H) intracellular IDO1 expression of surviving CD14 + CD11c + monocyte-derived DCs. (I) IL-10 cytokine production, and (J) %Treg (CD25 + CD127 − CD4 + T cells) induction in the ADCC assay as described in (C). Representative data from 1 of 3 donors are shown with error bars indicating mean ± SEM.

Journal: ImmunoHorizons

Article Title: Direct contact with natural killer cells reprograms monocyte-derived dendritic cells into a tolerogenic phenotype

doi: 10.1093/immhor/vlag007

Figure Lengend Snippet: Surviving monocyte-derived DCs following ADCC exhibit tolerogenic phenotypes. (A) Flow cytometry analysis of house-made anti-DCIR (clone 5E11) antibody binding to the CD14 + monocytes, B cells, T cells, and NK cells from human PBMCs ( n = 4 donors). Means ± SEM are shown, and statistical analysis is determined by one-way ANOVA test with Dunnett correction compared to the isotype staining control. ** P < .01. (B) Flow cytometry analysis of our anti-DCIR (5E11) antibody’s binding to the CD14 + monocytes, monocyte-derived DCs and macrophages ( n = 4 donors). (C) Study design of ADCC assay using anti-DCIR antibody to deplete monocyte-derived DCs differentiated and matured in vitro. (D) Cell count, (E–G) Mean Fluorescence Intensity (MFI) of surface co-stimulatory markers (CD11c, CD40, and CD44), and (H) intracellular IDO1 expression of surviving CD14 + CD11c + monocyte-derived DCs. (I) IL-10 cytokine production, and (J) %Treg (CD25 + CD127 − CD4 + T cells) induction in the ADCC assay as described in (C). Representative data from 1 of 3 donors are shown with error bars indicating mean ± SEM.

Techniques: Derivative Assay, Flow Cytometry, Analysis, Binding Assay, Staining, Control, ADCC Assay, In Vitro, Cell Characterization, Fluorescence, Expressing, FACS

Epigenetic remodeling of chromatin accessibility induces the transcriptional regulation of tolerogenic phenotypes in NK-programmed DCs. (A) Study design of bulk RNA-seq and ATAC-seq analysis on the surviving MoDCs from the NK–DC co-culture (weak NK engager) and MoDC cultured alone collected before and after LPS stimulation. (B) Principal component analysis (PCA) plot of the RNA-seq results using edgeR showing the 4 groups as defined in (A). (C) Gene expression changes identified by DGE analysis comparing the effects of LPS stimulation in DCs cultured alone or co-cultured with NK cells (weak NK engager). (D) Gene expression changes identified by DGE analysis comparing DC (weak NK-engager) vs DC alone. (E) Correlation of changes in chromatin accessibility determined by ATAC-seq and changes in gene expression determined by bulk RNA-seq in DCs alone compared to DCs co-cultured with NK cells (weak NK-engager). (F) Genome browser plots showing chromatin accessibility indicated by ATAC-seq peaks in proinflammatory cytokines genes (eg IL-1B, IL-6) and DC co-stimulatory marker genes (eg CD40 and CD11c) loci. Highlighted peaks indicate promoters that have lower accessibility in DC (weak NK-engager) as compared to DCs alone (DEseq, FDR <0.05). (G) Predicted top 20 most enriched transcription factors motifs based on the differentially chromatin accessible peaks identified by the ATAC-seq results.

Journal: ImmunoHorizons

Article Title: Direct contact with natural killer cells reprograms monocyte-derived dendritic cells into a tolerogenic phenotype

doi: 10.1093/immhor/vlag007

Figure Lengend Snippet: Epigenetic remodeling of chromatin accessibility induces the transcriptional regulation of tolerogenic phenotypes in NK-programmed DCs. (A) Study design of bulk RNA-seq and ATAC-seq analysis on the surviving MoDCs from the NK–DC co-culture (weak NK engager) and MoDC cultured alone collected before and after LPS stimulation. (B) Principal component analysis (PCA) plot of the RNA-seq results using edgeR showing the 4 groups as defined in (A). (C) Gene expression changes identified by DGE analysis comparing the effects of LPS stimulation in DCs cultured alone or co-cultured with NK cells (weak NK engager). (D) Gene expression changes identified by DGE analysis comparing DC (weak NK-engager) vs DC alone. (E) Correlation of changes in chromatin accessibility determined by ATAC-seq and changes in gene expression determined by bulk RNA-seq in DCs alone compared to DCs co-cultured with NK cells (weak NK-engager). (F) Genome browser plots showing chromatin accessibility indicated by ATAC-seq peaks in proinflammatory cytokines genes (eg IL-1B, IL-6) and DC co-stimulatory marker genes (eg CD40 and CD11c) loci. Highlighted peaks indicate promoters that have lower accessibility in DC (weak NK-engager) as compared to DCs alone (DEseq, FDR <0.05). (G) Predicted top 20 most enriched transcription factors motifs based on the differentially chromatin accessible peaks identified by the ATAC-seq results.

Techniques: RNA Sequencing, Analysis, Co-culture Assay, Cell Culture, Gene Expression, Marker, Co-Culture, Significance Assay

Journal: ImmunoHorizons

Article Title: Direct contact with natural killer cells reprograms monocyte-derived dendritic cells into a tolerogenic phenotype

doi: 10.1093/immhor/vlag007

Article Snippet: For the MLR experiment in , PBMCs were isolated from fresh human whole blood and treated with 40 ng/mL human IL-4 and 100 ng/mL human GM-CSF for 3 days to induce MoDC differentiation, followed by CpG ODN2006 (Invivogen, Cat# tlrl-2006) and CD40L (PeproTech, Cat#310-02) to promote MoDC maturation in addition to anti-DCIR antibody to induce antibody-dependent cellular cytotoxicity (ADCC) for 2 to 3 days.

Techniques: CRISPR, Activation Assay, Flow Cytometry, Expressing, Control, Cell Characterization, ADCC Assay

MNV recovery from whole Medjool dates and RT-qPCR inhibition.

Journal: Viruses

Article Title: Detection of Foodborne Viruses in Dates Using ISO 15216 Methodology

doi: 10.3390/v17020174

Figure Lengend Snippet: MNV recovery from whole Medjool dates and RT-qPCR inhibition.

Article Snippet: The viruses were extracted using the ISO 15216-1:2017 standard protocol version for soft fruit (ISO-modA) combined with the eGene-up RNA extraction process (Biomérieux, Montréal, QC, Canada), or the vegetable version combined with either the eGene-up (ISO-modB) or the RNeasy kit (ISO-modC) (Qiagen, Mississauga, ON, Canada) to extract the viral RNA following the manufacturer’s instructions.

Techniques: Inhibition

FK506 impairs the function of monocyte-derived Dendritic Cells (A) Alveolar macrophage and dendritic cell populations in bronchoalveolar lavage of patients with lung transplant with and without invasive aspergillosis ( n = 3). (B) Phagocytosis of A. fumigatus conidia (MOI = 1) by dendritic cells untreated, treated with IFNy or FK506 or both for 30 min ( n = 3). (C) Fungal killing of A. fumigatus conidia (MOI = 1) by dendritic cells untreated, treated with IFNy or FK506 or both 6 h post infection, measured by CFUs ( n = 3). (D) Representative ImageStream images of CD83 on moDCs treated with or without IFNy. (E) MFI of CD83 on moDCs untreated, treated with IFNy and/or FK506, infected with A. fumigatus (MOI = 1) or uninfected ( n = 3). (F) Representative ImageStream images of moDCs stained for nucleus (DAPI) and NFATc2 (Cy5), and infected with A. fumigatus (MOI = 1) or uninfected ( n = 3). (G) Timecourse of NFATc2 nuclear translocation of A. fumigatus infected (MOI = 1) moDCs treated with and without FK506 ( n = 3). (H) Time course RCAN-1 expression of Aspergillus infected (MOI = 1) moDCs DCs treated with and without FK506 ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 (A) t-test (B, C, E, and H) one-way ANOVA (G) two-way ANOVA. Data represented as mean ± SEM.

Journal: iScience

Article Title: Interferon-gamma rescues dendritic cell calcineurin-dependent responses to Aspergillus fumigatus via Stat3 to Stat1 switching

doi: 10.1016/j.isci.2024.111535

Figure Lengend Snippet: FK506 impairs the function of monocyte-derived Dendritic Cells (A) Alveolar macrophage and dendritic cell populations in bronchoalveolar lavage of patients with lung transplant with and without invasive aspergillosis ( n = 3). (B) Phagocytosis of A. fumigatus conidia (MOI = 1) by dendritic cells untreated, treated with IFNy or FK506 or both for 30 min ( n = 3). (C) Fungal killing of A. fumigatus conidia (MOI = 1) by dendritic cells untreated, treated with IFNy or FK506 or both 6 h post infection, measured by CFUs ( n = 3). (D) Representative ImageStream images of CD83 on moDCs treated with or without IFNy. (E) MFI of CD83 on moDCs untreated, treated with IFNy and/or FK506, infected with A. fumigatus (MOI = 1) or uninfected ( n = 3). (F) Representative ImageStream images of moDCs stained for nucleus (DAPI) and NFATc2 (Cy5), and infected with A. fumigatus (MOI = 1) or uninfected ( n = 3). (G) Timecourse of NFATc2 nuclear translocation of A. fumigatus infected (MOI = 1) moDCs treated with and without FK506 ( n = 3). (H) Time course RCAN-1 expression of Aspergillus infected (MOI = 1) moDCs DCs treated with and without FK506 ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 (A) t-test (B, C, E, and H) one-way ANOVA (G) two-way ANOVA. Data represented as mean ± SEM.

Article Snippet: MoDC’s at 1x10 7 per flask were pre-treated with FK506 and/or IFNγ and infected with swollen A. fumigatus conidia for 1 h. Flasks were pipetted-washed to harvest, with the aid of 10 min of cold 5 mM EDTA, and transferred to 1.5 mL Eppendorf tubes.

Techniques: Derivative Assay, Infection, Staining, Translocation Assay, Expressing

FK506 treatment abrogates T cell activation by Dendritic Cells (A) CD4 cell proliferative capacity measured in a mixed leukocyte reaction of T cells and moDC’s, with A. fumigatus infection (MOI = 1), FK506 and IFN-γ treatment ( n = 3). (B) CXCR3 surface expression on CD4 T cells co-cultured with moDC’s with A. fumigatus infection (MOI = 1), FK506 and IFN-γ treatment. (C and D) Cytokine secretion by moDCs or MDMs with A. fumigatus infection (MOI = 1), FK506 and IFN-γ treatment ( n = 3), (C) TNFa, (D) IL-2, (E) IL-10 and (F) IL-12. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 (A–F) one-way ANOVA. Data represented as mean ± SEM.

Journal: iScience

Article Title: Interferon-gamma rescues dendritic cell calcineurin-dependent responses to Aspergillus fumigatus via Stat3 to Stat1 switching

doi: 10.1016/j.isci.2024.111535

Figure Lengend Snippet: FK506 treatment abrogates T cell activation by Dendritic Cells (A) CD4 cell proliferative capacity measured in a mixed leukocyte reaction of T cells and moDC’s, with A. fumigatus infection (MOI = 1), FK506 and IFN-γ treatment ( n = 3). (B) CXCR3 surface expression on CD4 T cells co-cultured with moDC’s with A. fumigatus infection (MOI = 1), FK506 and IFN-γ treatment. (C and D) Cytokine secretion by moDCs or MDMs with A. fumigatus infection (MOI = 1), FK506 and IFN-γ treatment ( n = 3), (C) TNFa, (D) IL-2, (E) IL-10 and (F) IL-12. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 (A–F) one-way ANOVA. Data represented as mean ± SEM.

Techniques: Activation Assay, Infection, Expressing, Cell Culture

IFNγ and FK506 treatment alters the transcriptomic response to A. fumigatus in both moDCs and MDMs MoDC’s and MDM’s from healthy volunteers ( n = 3) were pre-treated with FK506 and/or IFNγ, then infected with A. fumigatus (MOI = 1) for 1 h prior to sequencing (A) Gene expression heatmap of top 200 genes (ranked by SD) per unclustered condition. (B) Venn diagram showing overlap of differentially expressed genes in infected moDCs and MDMs.

Journal: iScience

Article Title: Interferon-gamma rescues dendritic cell calcineurin-dependent responses to Aspergillus fumigatus via Stat3 to Stat1 switching

doi: 10.1016/j.isci.2024.111535

Figure Lengend Snippet: IFNγ and FK506 treatment alters the transcriptomic response to A. fumigatus in both moDCs and MDMs MoDC’s and MDM’s from healthy volunteers ( n = 3) were pre-treated with FK506 and/or IFNγ, then infected with A. fumigatus (MOI = 1) for 1 h prior to sequencing (A) Gene expression heatmap of top 200 genes (ranked by SD) per unclustered condition. (B) Venn diagram showing overlap of differentially expressed genes in infected moDCs and MDMs.

Techniques: Infection, Sequencing, Gene Expression

All genes differentially expressed with the FK506 treatment of A. fumigatus infected moDC’s and MDMs

Journal: iScience

Article Title: Interferon-gamma rescues dendritic cell calcineurin-dependent responses to Aspergillus fumigatus via Stat3 to Stat1 switching

doi: 10.1016/j.isci.2024.111535

Figure Lengend Snippet: All genes differentially expressed with the FK506 treatment of A. fumigatus infected moDC’s and MDMs

Techniques: Infection

Effects of IFNγ on moDC and MDM transcriptional responses during A. fumigatus infection (A) Numbers of differentially expressed genes with IFNγ treatment in moDC’s and MDM’s, with and without A. fumigatus infection. (B and C) Gene ontology of upregulated genes with IFNγ treatment in (B) moDCs and (C) MDMs. (D) Number of differentially expressed genes in infected cells with IFNγ treatment, with and without FK506 treatment.

Journal: iScience

Article Title: Interferon-gamma rescues dendritic cell calcineurin-dependent responses to Aspergillus fumigatus via Stat3 to Stat1 switching

doi: 10.1016/j.isci.2024.111535

Figure Lengend Snippet: Effects of IFNγ on moDC and MDM transcriptional responses during A. fumigatus infection (A) Numbers of differentially expressed genes with IFNγ treatment in moDC’s and MDM’s, with and without A. fumigatus infection. (B and C) Gene ontology of upregulated genes with IFNγ treatment in (B) moDCs and (C) MDMs. (D) Number of differentially expressed genes in infected cells with IFNγ treatment, with and without FK506 treatment.

Techniques: Infection

All genes differentially expressed by IFNγ treatment during the FK506 treatment of infected moDC’s and MDMs

Journal: iScience

Article Title: Interferon-gamma rescues dendritic cell calcineurin-dependent responses to Aspergillus fumigatus via Stat3 to Stat1 switching

doi: 10.1016/j.isci.2024.111535

Figure Lengend Snippet: All genes differentially expressed by IFNγ treatment during the FK506 treatment of infected moDC’s and MDMs

Techniques: Infection

FK506 and IFNy treatment alter the epigenetic landscape of A. fumigatus infected DCs (A) Sites of differential H3K27ac binding with the A. fumigatus infection of untreated moDC’s. (B and C) Genome-wide distribution of differential H3K27ac signal around nearest gene transcription start site (TSS, green line) with the A. fumigatus infection of untreated moDCs. (D) Sites of differential H3K27ac binding with the IFN-γ treatment of A. fumigatus infected FK506-treated moDCs. (E and F) Genome-wide distribution of differential H3K27ac signal with respect to nearest gene transcription start site (TSS, green line) with the IFN-γ treatment of A. fumigatus , FK506-treated moDCs.

Journal: iScience

Article Title: Interferon-gamma rescues dendritic cell calcineurin-dependent responses to Aspergillus fumigatus via Stat3 to Stat1 switching

doi: 10.1016/j.isci.2024.111535

Figure Lengend Snippet: FK506 and IFNy treatment alter the epigenetic landscape of A. fumigatus infected DCs (A) Sites of differential H3K27ac binding with the A. fumigatus infection of untreated moDC’s. (B and C) Genome-wide distribution of differential H3K27ac signal around nearest gene transcription start site (TSS, green line) with the A. fumigatus infection of untreated moDCs. (D) Sites of differential H3K27ac binding with the IFN-γ treatment of A. fumigatus infected FK506-treated moDCs. (E and F) Genome-wide distribution of differential H3K27ac signal with respect to nearest gene transcription start site (TSS, green line) with the IFN-γ treatment of A. fumigatus , FK506-treated moDCs.

Techniques: Infection, Binding Assay, Genome Wide

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human monocyte-derived dendritic cells (modcs) (AllCells LLC)

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