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DNA Chip Research Inc gene expression microarray
Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of <t>microarray</t> data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.
Gene Expression Microarray, supplied by DNA Chip Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Trisomy 12 compromises the mesendodermal differentiation propensity of human pluripotent stem cells

Journal: In Vitro Cellular & Developmental Biology. Animal

doi: 10.1007/s11626-023-00824-9

Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of microarray data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.
Figure Legend Snippet: Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of microarray data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.

Techniques Used: Comparison, Gene Expression, Microarray

Related Articles

Gene Expression:

Article Title: Trisomy 12 compromises the mesendodermal differentiation propensity of human pluripotent stem cells
Article Snippet: Data analysis was carried out using the Web-based hPSC Scorecard Analysis Software at www.lifetechnologies.com/scorecarddata . .. Gene expression microarray experiments were performed by DNA Chip Research Inc (Tokyo, Japan). .. After obtaining the genomic DNA-free total RNA described above, the RNA quantity and quality were verified with a NanoDrop 1000 (Thermo Fisher Scientific), Qubit 2.0 Fluorometer (Thermo Fisher Scientific), and Bioanalyzer RNA6000 Nano (Agilent, Santa Clara, CA).

Microarray:

Article Title: Trisomy 12 compromises the mesendodermal differentiation propensity of human pluripotent stem cells
Article Snippet: Data analysis was carried out using the Web-based hPSC Scorecard Analysis Software at www.lifetechnologies.com/scorecarddata . .. Gene expression microarray experiments were performed by DNA Chip Research Inc (Tokyo, Japan). .. After obtaining the genomic DNA-free total RNA described above, the RNA quantity and quality were verified with a NanoDrop 1000 (Thermo Fisher Scientific), Qubit 2.0 Fluorometer (Thermo Fisher Scientific), and Bioanalyzer RNA6000 Nano (Agilent, Santa Clara, CA).



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