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mng sequences  (Addgene inc)


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    Structured Review

    Addgene inc mng sequences
    Mng Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mng+sequence/mNG-mNG+(Plasmid+%2382729)/pm41339934-80-1-14
    Average 93 stars, based on 12 article reviews
    mng sequences - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Construct:

    Article Title: Phosphatidylinositol 4,5-bisphosphate impacts extracellular vesicle shedding from C. elegans ciliated sensory neurons
    Article Snippet: .. The construct used to express the PI(4,5)P 2 sensor mNG::PLCδ1-PH in the EVNs was created using restriction enzymes for cloning. mNG containing synthetic introns, with no stop codon, and followed by the flexible linker GSSGSSGTS was amplified from Addgene plasmid #177338 pGLOW77 ( ) and inserted into pENM1, a plasmid which contains the 1.58 kb klp-6 promoter ( ). .. The PH domain of phospholipase C δ1 ( ) was amplified from Addgene plasmid #21179 (GFP-C1-PLCdelta-PH) and inserted following the flexible linker at the C-terminal end of mNG.

    Article Title: Phosphatidylinositol (4,5)-bisphosphate Impacts Ectosome Shedding from C. elegans Ciliated Sensory Neurons
    Article Snippet: .. The construct used to express the PI(4,5)P 2 sensor mNG::PLCδ1-PH in the EVNs was created using restriction enzymes for cloning. mNG containing synthetic introns, with no stop codon, and followed by the flexible linker GSSGSSGTS was amplified from Addgene plasmid #177338 pGLOW77 ( ) and inserted into pENM1, a plasmid which contains the 1.58 kb klp-6 promoter ( ). .. The PH domain of phospholipase C delta 1 ( ) was amplified from Addgene plasmid #21179 (GFP-C1-PLCdelta-PH) and inserted following the flexible linker at the C-terminal end of mNG.

    Article Title: AI-Driven Virtual Screening and First-in-Class Conformational Biosensor Enable the Discovery of a GPR183 Inverse Agonist
    Article Snippet: .. HiBiT-GPR183-mNG-Nluc plasmid DNA constructs were generated using Gibson cloning, inserting mNeonGreen (mNG) from mNeonGreen-APEX plasmid DNA (#202591 Addgene, deposited by Reuben Harris) at three distinct positions into the GPR183 sequence (following P231, T233 or K235) in HiBiT-GPR183-Nluc. ..

    Cloning:

    Article Title: Phosphatidylinositol 4,5-bisphosphate impacts extracellular vesicle shedding from C. elegans ciliated sensory neurons
    Article Snippet: .. The construct used to express the PI(4,5)P 2 sensor mNG::PLCδ1-PH in the EVNs was created using restriction enzymes for cloning. mNG containing synthetic introns, with no stop codon, and followed by the flexible linker GSSGSSGTS was amplified from Addgene plasmid #177338 pGLOW77 ( ) and inserted into pENM1, a plasmid which contains the 1.58 kb klp-6 promoter ( ). .. The PH domain of phospholipase C δ1 ( ) was amplified from Addgene plasmid #21179 (GFP-C1-PLCdelta-PH) and inserted following the flexible linker at the C-terminal end of mNG.

    Article Title: Phosphatidylinositol (4,5)-bisphosphate Impacts Ectosome Shedding from C. elegans Ciliated Sensory Neurons
    Article Snippet: .. The construct used to express the PI(4,5)P 2 sensor mNG::PLCδ1-PH in the EVNs was created using restriction enzymes for cloning. mNG containing synthetic introns, with no stop codon, and followed by the flexible linker GSSGSSGTS was amplified from Addgene plasmid #177338 pGLOW77 ( ) and inserted into pENM1, a plasmid which contains the 1.58 kb klp-6 promoter ( ). .. The PH domain of phospholipase C delta 1 ( ) was amplified from Addgene plasmid #21179 (GFP-C1-PLCdelta-PH) and inserted following the flexible linker at the C-terminal end of mNG.

    Article Title: AI-Driven Virtual Screening and First-in-Class Conformational Biosensor Enable the Discovery of a GPR183 Inverse Agonist
    Article Snippet: .. HiBiT-GPR183-mNG-Nluc plasmid DNA constructs were generated using Gibson cloning, inserting mNeonGreen (mNG) from mNeonGreen-APEX plasmid DNA (#202591 Addgene, deposited by Reuben Harris) at three distinct positions into the GPR183 sequence (following P231, T233 or K235) in HiBiT-GPR183-Nluc. ..

    Amplification:

    Article Title: Phosphatidylinositol 4,5-bisphosphate impacts extracellular vesicle shedding from C. elegans ciliated sensory neurons
    Article Snippet: .. The construct used to express the PI(4,5)P 2 sensor mNG::PLCδ1-PH in the EVNs was created using restriction enzymes for cloning. mNG containing synthetic introns, with no stop codon, and followed by the flexible linker GSSGSSGTS was amplified from Addgene plasmid #177338 pGLOW77 ( ) and inserted into pENM1, a plasmid which contains the 1.58 kb klp-6 promoter ( ). .. The PH domain of phospholipase C δ1 ( ) was amplified from Addgene plasmid #21179 (GFP-C1-PLCdelta-PH) and inserted following the flexible linker at the C-terminal end of mNG.

    Article Title: Phosphatidylinositol (4,5)-bisphosphate Impacts Ectosome Shedding from C. elegans Ciliated Sensory Neurons
    Article Snippet: .. The construct used to express the PI(4,5)P 2 sensor mNG::PLCδ1-PH in the EVNs was created using restriction enzymes for cloning. mNG containing synthetic introns, with no stop codon, and followed by the flexible linker GSSGSSGTS was amplified from Addgene plasmid #177338 pGLOW77 ( ) and inserted into pENM1, a plasmid which contains the 1.58 kb klp-6 promoter ( ). .. The PH domain of phospholipase C delta 1 ( ) was amplified from Addgene plasmid #21179 (GFP-C1-PLCdelta-PH) and inserted following the flexible linker at the C-terminal end of mNG.

    Article Title: The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line.
    Article Snippet: .. Homology arms in the coding sequence of lin-5 and in the 30-UTR region were amplified using genomic DNA as a template. mAID was amplified from the plasmid pMK290 (Natsume et al.91; Addgene: #72828). mNG was amplified from a plasmid containing mNG-mom-5.86 The self-excising cassette (SEC) was amplified from the plasmid pDD287 (Dickinson et al.92, Addgene #70685). ..

    Plasmid Preparation:

    Article Title: Phosphatidylinositol 4,5-bisphosphate impacts extracellular vesicle shedding from C. elegans ciliated sensory neurons
    Article Snippet: .. The construct used to express the PI(4,5)P 2 sensor mNG::PLCδ1-PH in the EVNs was created using restriction enzymes for cloning. mNG containing synthetic introns, with no stop codon, and followed by the flexible linker GSSGSSGTS was amplified from Addgene plasmid #177338 pGLOW77 ( ) and inserted into pENM1, a plasmid which contains the 1.58 kb klp-6 promoter ( ). .. The PH domain of phospholipase C δ1 ( ) was amplified from Addgene plasmid #21179 (GFP-C1-PLCdelta-PH) and inserted following the flexible linker at the C-terminal end of mNG.

    Article Title: Towards enhancing photostability and adeno-associated viral vector delivery for genetically encoded plasma fluorescent labels
    Article Snippet: .. The DNA plasmids for pAAV-P3-Alb-mNG and pAAV-P3-Alb-RM, respectively, encoding mouse albumin tagged with mNeonGreen (mNG) or Rosmarinus (RM), have been published by Wang et al. and are available from Addgene (plasmid ID: 183460 and 183462, respectively). ..

    Article Title: Phosphatidylinositol (4,5)-bisphosphate Impacts Ectosome Shedding from C. elegans Ciliated Sensory Neurons
    Article Snippet: .. The construct used to express the PI(4,5)P 2 sensor mNG::PLCδ1-PH in the EVNs was created using restriction enzymes for cloning. mNG containing synthetic introns, with no stop codon, and followed by the flexible linker GSSGSSGTS was amplified from Addgene plasmid #177338 pGLOW77 ( ) and inserted into pENM1, a plasmid which contains the 1.58 kb klp-6 promoter ( ). .. The PH domain of phospholipase C delta 1 ( ) was amplified from Addgene plasmid #21179 (GFP-C1-PLCdelta-PH) and inserted following the flexible linker at the C-terminal end of mNG.

    Article Title: AI-Driven Virtual Screening and First-in-Class Conformational Biosensor Enable the Discovery of a GPR183 Inverse Agonist
    Article Snippet: .. HiBiT-GPR183-mNG-Nluc plasmid DNA constructs were generated using Gibson cloning, inserting mNeonGreen (mNG) from mNeonGreen-APEX plasmid DNA (#202591 Addgene, deposited by Reuben Harris) at three distinct positions into the GPR183 sequence (following P231, T233 or K235) in HiBiT-GPR183-Nluc. ..

    Article Title: The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line.
    Article Snippet: .. Homology arms in the coding sequence of lin-5 and in the 30-UTR region were amplified using genomic DNA as a template. mAID was amplified from the plasmid pMK290 (Natsume et al.91; Addgene: #72828). mNG was amplified from a plasmid containing mNG-mom-5.86 The self-excising cassette (SEC) was amplified from the plasmid pDD287 (Dickinson et al.92, Addgene #70685). ..

    Article Title: Genetically platform presenting CD24 ScFv enhance antitumor immunity by restoring macrophage phagocytosis and modulating the immune environment
    Article Snippet: The NanoLuc was acquired from the plasmid “pUAS-NanoLuc”, which was purchased from Addgene (Addgene #87696). .. The mNG sequences was acquired from the plasmid “pSFFV_mNG2 [ ]1–10”, which was purchased from Addgene (Addgene #82610). ..

    Article Title: Genetically platform presenting CD24 ScFv enhance antitumor immunity by restoring macrophage phagocytosis and modulating the immune environment.
    Article Snippet: The NanoLuc was acquired from the plasmid “pUAS-NanoLuc”, which was purchased from Addgene (Addgene #87696). .. The mNG sequences was acquired from the plasmid “pSFFV_mNG2 [11]1–10”, which was purchased from Addgene (Addgene #82610). ..

    Article Title: Real-Time In Vivo Detection of Nanocarrier Number and Velocity in the Cerebrovasculature Using Hot Band Absorption.
    Article Snippet: Nanocarriers play a critical role in the transport and targeted delivery of therapeutic agents.. However, real-time in vivo observation of individual nanocarriers in the circulatory system is challenging due to the need for high spatiotemporal resolution and sensitivity.. Here, DNA-stabilized silver nanoclusters (DNA640), which exhibit anti-Stokes fluorescence upon hot-band absorption (HBA), are loaded into cationic mesoporous silica nanoparticles (CMSN) and coated with liposomes.

    Generated:

    Article Title: AI-Driven Virtual Screening and First-in-Class Conformational Biosensor Enable the Discovery of a GPR183 Inverse Agonist
    Article Snippet: .. HiBiT-GPR183-mNG-Nluc plasmid DNA constructs were generated using Gibson cloning, inserting mNeonGreen (mNG) from mNeonGreen-APEX plasmid DNA (#202591 Addgene, deposited by Reuben Harris) at three distinct positions into the GPR183 sequence (following P231, T233 or K235) in HiBiT-GPR183-Nluc. ..

    Sequencing:

    Article Title: AI-Driven Virtual Screening and First-in-Class Conformational Biosensor Enable the Discovery of a GPR183 Inverse Agonist
    Article Snippet: .. HiBiT-GPR183-mNG-Nluc plasmid DNA constructs were generated using Gibson cloning, inserting mNeonGreen (mNG) from mNeonGreen-APEX plasmid DNA (#202591 Addgene, deposited by Reuben Harris) at three distinct positions into the GPR183 sequence (following P231, T233 or K235) in HiBiT-GPR183-Nluc. ..

    Article Title: The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line.
    Article Snippet: .. Homology arms in the coding sequence of lin-5 and in the 30-UTR region were amplified using genomic DNA as a template. mAID was amplified from the plasmid pMK290 (Natsume et al.91; Addgene: #72828). mNG was amplified from a plasmid containing mNG-mom-5.86 The self-excising cassette (SEC) was amplified from the plasmid pDD287 (Dickinson et al.92, Addgene #70685). ..



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    Image Search Results


    mNGS workflow in clinical application. CNS, Central nervous system.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases

    doi: 10.3389/fcimb.2024.1458316

    Figure Lengend Snippet: mNGS workflow in clinical application. CNS, Central nervous system.

    Article Snippet: The most commonly used mNGS sequencing platform is the Illumina platform, which is based on the core principle of sequencing by synthesis (SBS), which consists of four main steps: DNA library construction, BALF fluid Flowcell adsorption, bridge polymerase chain reaction (PCR) amplification, and SBS ( ).

    Techniques:

    The comparison between  mNGS  and tNGS.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases

    doi: 10.3389/fcimb.2024.1458316

    Figure Lengend Snippet: The comparison between mNGS and tNGS.

    Article Snippet: The most commonly used mNGS sequencing platform is the Illumina platform, which is based on the core principle of sequencing by synthesis (SBS), which consists of four main steps: DNA library construction, BALF fluid Flowcell adsorption, bridge polymerase chain reaction (PCR) amplification, and SBS ( ).

    Techniques: Comparison, Extraction, Next-Generation Sequencing, Sequencing, Mutagenesis, Bacteria

    Comparison of testing methods for diagnosing infectious diseases.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases

    doi: 10.3389/fcimb.2024.1458316

    Figure Lengend Snippet: Comparison of testing methods for diagnosing infectious diseases.

    Article Snippet: The most commonly used mNGS sequencing platform is the Illumina platform, which is based on the core principle of sequencing by synthesis (SBS), which consists of four main steps: DNA library construction, BALF fluid Flowcell adsorption, bridge polymerase chain reaction (PCR) amplification, and SBS ( ).

    Techniques: Comparison, Microscopy, Staining, Mass Spectrometry, Real-time Polymerase Chain Reaction, Multiplex Assay, Sequencing, Multiplexing, Quantitation Assay, Amplification, Bacteria, Generated, Functional Assay, Binding Assay