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mmp13 antibody  (Proteintech)


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    Proteintech mmp13 antibody
    The upregulation of CK in the cartilage and meniscus during early OA. (a) The contents of CK in the synovial fluid, articular cartilage and meniscus tissue of OA patients are measured with an ELISA kit. n = 20, one-way ANOVA, ∗∗∗∗ represents p < 0.0001. (b, c) Immunofluorescence staining (b) and semi-quantitative analysis (c) of CK levels within synovial fluid, articular cartilage and meniscus tissues of OA patients. CK represents creatine kinase. n = 6, one-way ANOVA, ∗∗ represents p < 0.01 and ∗∗∗∗ represents p < 0.0001. White scale bar: 50 μm; yellow scale bar: 10 μm. (d, e) Immunofluorescence staining (d) and semi-quantitative analysis (e) of CK levels in the knee joints of normal, early OA and late OA mice. F represents the femur, T represents the tibia, M represents the meniscus, AC represents the articular cartilage and SB represents the subchondral bone. n = 6, one-way ANOVA, ∗∗∗∗ represents p < 0.0001. White scale bar: 50 μm; yellow scale bar: 10 μm. (f – h) SDS-PAGE (f) results and semi-quantitative analysis of CK (g) and <t>MMP13</t> (h) levels in rat chondrocytes after in vitro LPS stimulation for different durations. n = 3, one-way ANOVA, ∗ represents p < 0.05, ∗∗ represents p < 0.01, ∗∗∗ represents p < 0.0005, ∗∗∗∗ represents p < 0.0001 and ns represents no significant difference. MMP13 represents matrix metalloproteinase-13, and CK represents creatine kinase. (i – k) SDS‒PAGE results (i) and semi-quantitative analysis of CK (j) and MMP13 (k) levels in rat meniscus fibrochondrocytes after in vitro LPS stimulation for different durations. n = 3, one-way ANOVA, ∗∗ represents p < 0.01, ∗∗∗ represents p < 0.0005, ∗∗∗∗ represents p < 0.0001 and ns represents no significant difference.
    Mmp13 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmp13/MMP13+Antibody/pmc12972744-490-3-8
    Average 96 stars, based on 806 article reviews
    mmp13 antibody - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Artificial mitochondria ameliorates osteoarthritis through restoring cellular energy metabolism homeostasis"

    Article Title: Artificial mitochondria ameliorates osteoarthritis through restoring cellular energy metabolism homeostasis

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.028

    The upregulation of CK in the cartilage and meniscus during early OA. (a) The contents of CK in the synovial fluid, articular cartilage and meniscus tissue of OA patients are measured with an ELISA kit. n = 20, one-way ANOVA, ∗∗∗∗ represents p < 0.0001. (b, c) Immunofluorescence staining (b) and semi-quantitative analysis (c) of CK levels within synovial fluid, articular cartilage and meniscus tissues of OA patients. CK represents creatine kinase. n = 6, one-way ANOVA, ∗∗ represents p < 0.01 and ∗∗∗∗ represents p < 0.0001. White scale bar: 50 μm; yellow scale bar: 10 μm. (d, e) Immunofluorescence staining (d) and semi-quantitative analysis (e) of CK levels in the knee joints of normal, early OA and late OA mice. F represents the femur, T represents the tibia, M represents the meniscus, AC represents the articular cartilage and SB represents the subchondral bone. n = 6, one-way ANOVA, ∗∗∗∗ represents p < 0.0001. White scale bar: 50 μm; yellow scale bar: 10 μm. (f – h) SDS-PAGE (f) results and semi-quantitative analysis of CK (g) and MMP13 (h) levels in rat chondrocytes after in vitro LPS stimulation for different durations. n = 3, one-way ANOVA, ∗ represents p < 0.05, ∗∗ represents p < 0.01, ∗∗∗ represents p < 0.0005, ∗∗∗∗ represents p < 0.0001 and ns represents no significant difference. MMP13 represents matrix metalloproteinase-13, and CK represents creatine kinase. (i – k) SDS‒PAGE results (i) and semi-quantitative analysis of CK (j) and MMP13 (k) levels in rat meniscus fibrochondrocytes after in vitro LPS stimulation for different durations. n = 3, one-way ANOVA, ∗∗ represents p < 0.01, ∗∗∗ represents p < 0.0005, ∗∗∗∗ represents p < 0.0001 and ns represents no significant difference.
    Figure Legend Snippet: The upregulation of CK in the cartilage and meniscus during early OA. (a) The contents of CK in the synovial fluid, articular cartilage and meniscus tissue of OA patients are measured with an ELISA kit. n = 20, one-way ANOVA, ∗∗∗∗ represents p < 0.0001. (b, c) Immunofluorescence staining (b) and semi-quantitative analysis (c) of CK levels within synovial fluid, articular cartilage and meniscus tissues of OA patients. CK represents creatine kinase. n = 6, one-way ANOVA, ∗∗ represents p < 0.01 and ∗∗∗∗ represents p < 0.0001. White scale bar: 50 μm; yellow scale bar: 10 μm. (d, e) Immunofluorescence staining (d) and semi-quantitative analysis (e) of CK levels in the knee joints of normal, early OA and late OA mice. F represents the femur, T represents the tibia, M represents the meniscus, AC represents the articular cartilage and SB represents the subchondral bone. n = 6, one-way ANOVA, ∗∗∗∗ represents p < 0.0001. White scale bar: 50 μm; yellow scale bar: 10 μm. (f – h) SDS-PAGE (f) results and semi-quantitative analysis of CK (g) and MMP13 (h) levels in rat chondrocytes after in vitro LPS stimulation for different durations. n = 3, one-way ANOVA, ∗ represents p < 0.05, ∗∗ represents p < 0.01, ∗∗∗ represents p < 0.0005, ∗∗∗∗ represents p < 0.0001 and ns represents no significant difference. MMP13 represents matrix metalloproteinase-13, and CK represents creatine kinase. (i – k) SDS‒PAGE results (i) and semi-quantitative analysis of CK (j) and MMP13 (k) levels in rat meniscus fibrochondrocytes after in vitro LPS stimulation for different durations. n = 3, one-way ANOVA, ∗∗ represents p < 0.01, ∗∗∗ represents p < 0.0005, ∗∗∗∗ represents p < 0.0001 and ns represents no significant difference.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, SDS Page, In Vitro

    Related Articles

    Incubation:

    Article Title: Therapeutic effects of Tetramethylpyrazine on Cartilage in Rat Model of Post-traumatic Osteoarthritis
    Article Snippet: .. The sections were then incubated with the following primary antibodies: anti- ADAMTS5 (1:100, BS-3573R, Biosharp Co.,Ltd, Beijing, China), anti-COL II (1:100, ab34712, Abcam, Cambridge, MA, USA), and anti-MMP13 (1:50, 18165-1-AP, Proteintech Group, Rosemount, USA). ..

    Article Title: Therapeutic effects of hypoxia-preconditioned cartilage progenitor cell-exosomes on osteoarthritis through autophagy activation and macrophage polarization modulation
    Article Snippet: .. Membranes were blocked with 5% bovine serum albumin (BSA) for 30 min and incubated overnight at 4 °C with the following primary antibodies: Collagen II (COL II; Santa Cruz, USA), Aggrecan (Proteintech, USA), MMP13 (Proteintech, USA), SOX9 (Abcam, USA), Rab1A (Abcam, USA), p-mTOR (CST, USA), mTOR (CST, USA), p-S6K (CST, USA), S6K (CST, USA), p-4E-BP1(Abcam, USA), 4E-BP1(Abcam, USA), Beclin1 (Abcam, USA), P62 (Abcam, USA), LC3 I/II (CST, USA), p-IκBα (CST, USA), IκBα (CST, USA), p-P65 (CST, USA), P65 (CST, USA), and β-actin (Abcam, USA). ..

    Article Title: Injectable microgels carrying engineered biomimetic nanoparticles for osteoarthritis therapy via dual-targeted senescent chondrocyte clearance and endogenous repair promotion
    Article Snippet: Inter-rater reliability was evaluated using the intraclass correlation coefficient (ICC), calculated based on a two-way random-effects model. For immunohistochemistry, antigen retrieval was conducted using EDTA Antigen Retrieval Solution (Solarbio, China) at 55 °C for 8 h. Endogenous peroxidase activity was blocked with 3 % H 2 O 2 for 10 min, followed by 1-h blocking in PBS containing 20 % donkey serum and 0.3 % Triton X-100. .. Then, sections were incubated overnight at 4 °C with primary antibodies: anti-COL2A1 (28459-1-AP, 1:200, Proteintech), anti-MMP13 (18165-1-AP, 1:200, Proteintech), anti-γ-H2AX (A2082, 1:100, ABclonal), anti-p16 (ab211542, 1:200, Abcam), anti-IL-1β (A27676, 1:200, ABclonal), anti-iNOS (22226-1-AP, 1:200, Proteintech). .. After TBST washes, sections were incubated with universal secondary antibody (Report biotechnology, China) for 1 h at room temperature.

    Article Title: Sequential delivery of sinigrin and dabigatran by an in situ self-stabilizing dynamic hydrogel attenuates intervertebral disc degeneration
    Article Snippet: .. Cells in glass-bottomed dishes were sequentially treated: fixed with 4 % paraformaldehyde for 20 min, permeabilized with 0.5 % Triton X-100 for 15 min, and blocked with 5 % (w/v) bovine serum albumin for 30 min, before incubation with primary antibodies against Col2a1 (1:100, A19308, abclone, China), MMP13 (A1606, abclone, China), COX2 (27308-1-AP, Proteintech, China). .. After overnight incubation at 4 °C, the specimens were stained with secondary antibodies conjugated to Alexa 488 or Cy3 for 1 h at room temperature and examined using an Olympus fluorescence microscope.

    Article Title: Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis
    Article Snippet: .. Cells were fixed with ice-cold methanol for 5 min and then blocked with 5% bovine serum albumin for 1 h. Samples were subsequently incubated with primary antibodies against COL2 (1:200, ProteinTech, 28459-1-AP) or MMP13 (1:200, ProteinTech, 18165-1-AP) overnight at 4 °C, followed by incubation with fluorophore-conjugated secondary antibodies for 1 h at room temperature. ..

    Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy
    Article Snippet: .. For immunofluorescence (IF) staining, antigen retrieval was conducted using citrate buffer (0.1 M, pH 6.0), followed by blocking in 10 % normal goat serum (Solarbio, Cat. No. SL038) at room temperature for 1 h. Sections were incubated overnight at 4 °C with primary antibodies targeting ACAN (Cat. No. DF7561, Affinity), COL2A1 (Cat. No. AF0135, Affinity), MMP3 (Cat. No. AF0217, Affinity), MMP13 (Cat. No. 18165-1-AP, Proteintech), CD31 (Cat. No. AF0077, Affinity), p-LATS1 (Cat. No. AF7170, Affinity), p-YAP (Cat. No. AF3328, Affinity), Tuj1 (Cat. No. 66375, Proteintech), CGRP (Cat. No. DF7386, Affinity), SP (Cat. No. DF7522, Affinity), and PGP9.5 (Cat. No. AF5490, Affinity) followed by incubation with appropriate fluorescent secondary antibodies for 2 h at room temperature and counterstaining with DAPI (Beyotime, Cat. No. C1006). .. Fluorescence images were acquired using a BX53 microscope (Olympus), and signal intensity was quantified with ImageJ software.

    Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression
    Article Snippet: For immunohistochemistry (IHC), tissue sections underwent sequential antigen retrieval and blocking using a high-power microwave treatment, endogenous peroxidase blocker (Biocare Medical, USA), and 5 % sheep serum (Beyotime, China). .. Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and MMP-13 (18165-1-AP; 1:100; Proteintech), followed by the corresponding secondary antibodies. .. HE, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), and IHC stained sections were scanned using a Pannoramic MIDI (3D HISTECH, Hungary), whereas EdU-stained sections were acquired using a BX53 microscope.

    other:

    Article Title: Artificial mitochondria ameliorates osteoarthritis through restoring cellular energy metabolism homeostasis
    Article Snippet: The rabbit anti MMP13 antibody was purchased from Proteintech (18165-1-AP).

    Immunofluorescence:

    Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy
    Article Snippet: .. For immunofluorescence (IF) staining, antigen retrieval was conducted using citrate buffer (0.1 M, pH 6.0), followed by blocking in 10 % normal goat serum (Solarbio, Cat. No. SL038) at room temperature for 1 h. Sections were incubated overnight at 4 °C with primary antibodies targeting ACAN (Cat. No. DF7561, Affinity), COL2A1 (Cat. No. AF0135, Affinity), MMP3 (Cat. No. AF0217, Affinity), MMP13 (Cat. No. 18165-1-AP, Proteintech), CD31 (Cat. No. AF0077, Affinity), p-LATS1 (Cat. No. AF7170, Affinity), p-YAP (Cat. No. AF3328, Affinity), Tuj1 (Cat. No. 66375, Proteintech), CGRP (Cat. No. DF7386, Affinity), SP (Cat. No. DF7522, Affinity), and PGP9.5 (Cat. No. AF5490, Affinity) followed by incubation with appropriate fluorescent secondary antibodies for 2 h at room temperature and counterstaining with DAPI (Beyotime, Cat. No. C1006). .. Fluorescence images were acquired using a BX53 microscope (Olympus), and signal intensity was quantified with ImageJ software.

    Staining:

    Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy
    Article Snippet: .. For immunofluorescence (IF) staining, antigen retrieval was conducted using citrate buffer (0.1 M, pH 6.0), followed by blocking in 10 % normal goat serum (Solarbio, Cat. No. SL038) at room temperature for 1 h. Sections were incubated overnight at 4 °C with primary antibodies targeting ACAN (Cat. No. DF7561, Affinity), COL2A1 (Cat. No. AF0135, Affinity), MMP3 (Cat. No. AF0217, Affinity), MMP13 (Cat. No. 18165-1-AP, Proteintech), CD31 (Cat. No. AF0077, Affinity), p-LATS1 (Cat. No. AF7170, Affinity), p-YAP (Cat. No. AF3328, Affinity), Tuj1 (Cat. No. 66375, Proteintech), CGRP (Cat. No. DF7386, Affinity), SP (Cat. No. DF7522, Affinity), and PGP9.5 (Cat. No. AF5490, Affinity) followed by incubation with appropriate fluorescent secondary antibodies for 2 h at room temperature and counterstaining with DAPI (Beyotime, Cat. No. C1006). .. Fluorescence images were acquired using a BX53 microscope (Olympus), and signal intensity was quantified with ImageJ software.

    Blocking Assay:

    Article Title: An intelligent nanoliposome alleviates disc degeneration and discogenic pain by inhibiting neurovascular ingrowth via a “Soil-conditioning, seed-modulating, and weeds-suppressing” strategy
    Article Snippet: .. For immunofluorescence (IF) staining, antigen retrieval was conducted using citrate buffer (0.1 M, pH 6.0), followed by blocking in 10 % normal goat serum (Solarbio, Cat. No. SL038) at room temperature for 1 h. Sections were incubated overnight at 4 °C with primary antibodies targeting ACAN (Cat. No. DF7561, Affinity), COL2A1 (Cat. No. AF0135, Affinity), MMP3 (Cat. No. AF0217, Affinity), MMP13 (Cat. No. 18165-1-AP, Proteintech), CD31 (Cat. No. AF0077, Affinity), p-LATS1 (Cat. No. AF7170, Affinity), p-YAP (Cat. No. AF3328, Affinity), Tuj1 (Cat. No. 66375, Proteintech), CGRP (Cat. No. DF7386, Affinity), SP (Cat. No. DF7522, Affinity), and PGP9.5 (Cat. No. AF5490, Affinity) followed by incubation with appropriate fluorescent secondary antibodies for 2 h at room temperature and counterstaining with DAPI (Beyotime, Cat. No. C1006). .. Fluorescence images were acquired using a BX53 microscope (Olympus), and signal intensity was quantified with ImageJ software.



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    94
    Bioss rabbit anti mmp13 antibody
    Non-selective Nav1.7 inhibitors regulate chondrocyte metabolism . (A) mRNA levels of COL2 and ACAN in primary human OA chondrocytes treated with 10 μM LCM, CBZ, or OXC for 24h. (B) mRNA levels of <t>MMP13</t> and ADAMTS5 in primary human OA chondrocytes stimulated with 20 ng/mL IL-1β and 10 μM LCM, CBZ or OXC for 24h. (C, D) COL2 and ACAN (C) , MMP13 and ADAMTS5 (D) mRNA expression in human OA chondrocytes treated with various doses of LCM with/without IL-1β stimulated conditions. (E, F) mRNA levels of anabolic markers Col2 and Acan , as well as catabolic enzymes Mmp13 and Adamts5 , in primary chondrocytes isolated from WT and chondrocyte specific Nav1.7 knockout mice under basal conditions or following stimulation with 20 ng/mL IL-1β, with or without 10 μM LCM co-treatment for 24 h. (G) Representative immunofluorescence staining showing COL2 (green) and MMP13 (green) expression in C28/I2 chondrocytes treated with 20 ng/ml IL-1β and 10 nM LCM for 48h. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (H) Quantification of average optical density (AOD) shown in G. (I) Safranin O/Fast Green and IHC staining of human OA cartilage explants cultured with 20 ng/ml IL-1β and 10 nM LCM for 5 days. Scale bar = 100 μm. (J) Quantification of GAG area and OARSI scores in I. (K) Quantification of IHC staining in I. Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (A-F and H, J, K).
    Rabbit Anti Mmp13 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmp13/MMP-13+Polyclonal+Antibody/pm42049724-354-48-52
    Average 94 stars, based on 1 article reviews
    rabbit anti mmp13 antibody - by Bioz Stars, 2026-10
    94/100 stars
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    gene exp mmp13 hs00942584 m1  (Thermo Fisher)
    86
    Thermo Fisher gene exp mmp13 hs00942584 m1
    Non-selective Nav1.7 inhibitors regulate chondrocyte metabolism . (A) mRNA levels of COL2 and ACAN in primary human OA chondrocytes treated with 10 μM LCM, CBZ, or OXC for 24h. (B) mRNA levels of <t>MMP13</t> and ADAMTS5 in primary human OA chondrocytes stimulated with 20 ng/mL IL-1β and 10 μM LCM, CBZ or OXC for 24h. (C, D) COL2 and ACAN (C) , MMP13 and ADAMTS5 (D) mRNA expression in human OA chondrocytes treated with various doses of LCM with/without IL-1β stimulated conditions. (E, F) mRNA levels of anabolic markers Col2 and Acan , as well as catabolic enzymes Mmp13 and Adamts5 , in primary chondrocytes isolated from WT and chondrocyte specific Nav1.7 knockout mice under basal conditions or following stimulation with 20 ng/mL IL-1β, with or without 10 μM LCM co-treatment for 24 h. (G) Representative immunofluorescence staining showing COL2 (green) and MMP13 (green) expression in C28/I2 chondrocytes treated with 20 ng/ml IL-1β and 10 nM LCM for 48h. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (H) Quantification of average optical density (AOD) shown in G. (I) Safranin O/Fast Green and IHC staining of human OA cartilage explants cultured with 20 ng/ml IL-1β and 10 nM LCM for 5 days. Scale bar = 100 μm. (J) Quantification of GAG area and OARSI scores in I. (K) Quantification of IHC staining in I. Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (A-F and H, J, K).
    Gene Exp Mmp13 Hs00942584 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmp13/Gene+Exp%2E+MMP13%2C+Hs00942584_m1/pm42019646-170-31-12
    Average 86 stars, based on 1 article reviews
    gene exp mmp13 hs00942584 m1 - by Bioz Stars, 2026-10
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    antibodies against mmp13  (Bioss)
    94
    Bioss antibodies against mmp13
    Non-selective Nav1.7 inhibitors regulate chondrocyte metabolism . (A) mRNA levels of COL2 and ACAN in primary human OA chondrocytes treated with 10 μM LCM, CBZ, or OXC for 24h. (B) mRNA levels of <t>MMP13</t> and ADAMTS5 in primary human OA chondrocytes stimulated with 20 ng/mL IL-1β and 10 μM LCM, CBZ or OXC for 24h. (C, D) COL2 and ACAN (C) , MMP13 and ADAMTS5 (D) mRNA expression in human OA chondrocytes treated with various doses of LCM with/without IL-1β stimulated conditions. (E, F) mRNA levels of anabolic markers Col2 and Acan , as well as catabolic enzymes Mmp13 and Adamts5 , in primary chondrocytes isolated from WT and chondrocyte specific Nav1.7 knockout mice under basal conditions or following stimulation with 20 ng/mL IL-1β, with or without 10 μM LCM co-treatment for 24 h. (G) Representative immunofluorescence staining showing COL2 (green) and MMP13 (green) expression in C28/I2 chondrocytes treated with 20 ng/ml IL-1β and 10 nM LCM for 48h. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (H) Quantification of average optical density (AOD) shown in G. (I) Safranin O/Fast Green and IHC staining of human OA cartilage explants cultured with 20 ng/ml IL-1β and 10 nM LCM for 5 days. Scale bar = 100 μm. (J) Quantification of GAG area and OARSI scores in I. (K) Quantification of IHC staining in I. Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (A-F and H, J, K).
    Antibodies Against Mmp13, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmp13/MMP13+Polyclonal+Antibody/10__3390_slash_ijms27083618-180-60-65
    Average 94 stars, based on 1 article reviews
    antibodies against mmp13 - by Bioz Stars, 2026-10
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    Image Search Results


    The upregulation of CK in the cartilage and meniscus during early OA. (a) The contents of CK in the synovial fluid, articular cartilage and meniscus tissue of OA patients are measured with an ELISA kit. n = 20, one-way ANOVA, ∗∗∗∗ represents p < 0.0001. (b, c) Immunofluorescence staining (b) and semi-quantitative analysis (c) of CK levels within synovial fluid, articular cartilage and meniscus tissues of OA patients. CK represents creatine kinase. n = 6, one-way ANOVA, ∗∗ represents p < 0.01 and ∗∗∗∗ represents p < 0.0001. White scale bar: 50 μm; yellow scale bar: 10 μm. (d, e) Immunofluorescence staining (d) and semi-quantitative analysis (e) of CK levels in the knee joints of normal, early OA and late OA mice. F represents the femur, T represents the tibia, M represents the meniscus, AC represents the articular cartilage and SB represents the subchondral bone. n = 6, one-way ANOVA, ∗∗∗∗ represents p < 0.0001. White scale bar: 50 μm; yellow scale bar: 10 μm. (f – h) SDS-PAGE (f) results and semi-quantitative analysis of CK (g) and MMP13 (h) levels in rat chondrocytes after in vitro LPS stimulation for different durations. n = 3, one-way ANOVA, ∗ represents p < 0.05, ∗∗ represents p < 0.01, ∗∗∗ represents p < 0.0005, ∗∗∗∗ represents p < 0.0001 and ns represents no significant difference. MMP13 represents matrix metalloproteinase-13, and CK represents creatine kinase. (i – k) SDS‒PAGE results (i) and semi-quantitative analysis of CK (j) and MMP13 (k) levels in rat meniscus fibrochondrocytes after in vitro LPS stimulation for different durations. n = 3, one-way ANOVA, ∗∗ represents p < 0.01, ∗∗∗ represents p < 0.0005, ∗∗∗∗ represents p < 0.0001 and ns represents no significant difference.

    Journal: Bioactive Materials

    Article Title: Artificial mitochondria ameliorates osteoarthritis through restoring cellular energy metabolism homeostasis

    doi: 10.1016/j.bioactmat.2026.02.028

    Figure Lengend Snippet: The upregulation of CK in the cartilage and meniscus during early OA. (a) The contents of CK in the synovial fluid, articular cartilage and meniscus tissue of OA patients are measured with an ELISA kit. n = 20, one-way ANOVA, ∗∗∗∗ represents p < 0.0001. (b, c) Immunofluorescence staining (b) and semi-quantitative analysis (c) of CK levels within synovial fluid, articular cartilage and meniscus tissues of OA patients. CK represents creatine kinase. n = 6, one-way ANOVA, ∗∗ represents p < 0.01 and ∗∗∗∗ represents p < 0.0001. White scale bar: 50 μm; yellow scale bar: 10 μm. (d, e) Immunofluorescence staining (d) and semi-quantitative analysis (e) of CK levels in the knee joints of normal, early OA and late OA mice. F represents the femur, T represents the tibia, M represents the meniscus, AC represents the articular cartilage and SB represents the subchondral bone. n = 6, one-way ANOVA, ∗∗∗∗ represents p < 0.0001. White scale bar: 50 μm; yellow scale bar: 10 μm. (f – h) SDS-PAGE (f) results and semi-quantitative analysis of CK (g) and MMP13 (h) levels in rat chondrocytes after in vitro LPS stimulation for different durations. n = 3, one-way ANOVA, ∗ represents p < 0.05, ∗∗ represents p < 0.01, ∗∗∗ represents p < 0.0005, ∗∗∗∗ represents p < 0.0001 and ns represents no significant difference. MMP13 represents matrix metalloproteinase-13, and CK represents creatine kinase. (i – k) SDS‒PAGE results (i) and semi-quantitative analysis of CK (j) and MMP13 (k) levels in rat meniscus fibrochondrocytes after in vitro LPS stimulation for different durations. n = 3, one-way ANOVA, ∗∗ represents p < 0.01, ∗∗∗ represents p < 0.0005, ∗∗∗∗ represents p < 0.0001 and ns represents no significant difference.

    Article Snippet: The rabbit anti MMP13 antibody was purchased from Proteintech (18165-1-AP).

    Techniques: Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, SDS Page, In Vitro

    Non-selective Nav1.7 inhibitors regulate chondrocyte metabolism . (A) mRNA levels of COL2 and ACAN in primary human OA chondrocytes treated with 10 μM LCM, CBZ, or OXC for 24h. (B) mRNA levels of MMP13 and ADAMTS5 in primary human OA chondrocytes stimulated with 20 ng/mL IL-1β and 10 μM LCM, CBZ or OXC for 24h. (C, D) COL2 and ACAN (C) , MMP13 and ADAMTS5 (D) mRNA expression in human OA chondrocytes treated with various doses of LCM with/without IL-1β stimulated conditions. (E, F) mRNA levels of anabolic markers Col2 and Acan , as well as catabolic enzymes Mmp13 and Adamts5 , in primary chondrocytes isolated from WT and chondrocyte specific Nav1.7 knockout mice under basal conditions or following stimulation with 20 ng/mL IL-1β, with or without 10 μM LCM co-treatment for 24 h. (G) Representative immunofluorescence staining showing COL2 (green) and MMP13 (green) expression in C28/I2 chondrocytes treated with 20 ng/ml IL-1β and 10 nM LCM for 48h. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (H) Quantification of average optical density (AOD) shown in G. (I) Safranin O/Fast Green and IHC staining of human OA cartilage explants cultured with 20 ng/ml IL-1β and 10 nM LCM for 5 days. Scale bar = 100 μm. (J) Quantification of GAG area and OARSI scores in I. (K) Quantification of IHC staining in I. Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (A-F and H, J, K).

    Journal: Bioactive Materials

    Article Title: Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis

    doi: 10.1016/j.bioactmat.2026.02.045

    Figure Lengend Snippet: Non-selective Nav1.7 inhibitors regulate chondrocyte metabolism . (A) mRNA levels of COL2 and ACAN in primary human OA chondrocytes treated with 10 μM LCM, CBZ, or OXC for 24h. (B) mRNA levels of MMP13 and ADAMTS5 in primary human OA chondrocytes stimulated with 20 ng/mL IL-1β and 10 μM LCM, CBZ or OXC for 24h. (C, D) COL2 and ACAN (C) , MMP13 and ADAMTS5 (D) mRNA expression in human OA chondrocytes treated with various doses of LCM with/without IL-1β stimulated conditions. (E, F) mRNA levels of anabolic markers Col2 and Acan , as well as catabolic enzymes Mmp13 and Adamts5 , in primary chondrocytes isolated from WT and chondrocyte specific Nav1.7 knockout mice under basal conditions or following stimulation with 20 ng/mL IL-1β, with or without 10 μM LCM co-treatment for 24 h. (G) Representative immunofluorescence staining showing COL2 (green) and MMP13 (green) expression in C28/I2 chondrocytes treated with 20 ng/ml IL-1β and 10 nM LCM for 48h. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (H) Quantification of average optical density (AOD) shown in G. (I) Safranin O/Fast Green and IHC staining of human OA cartilage explants cultured with 20 ng/ml IL-1β and 10 nM LCM for 5 days. Scale bar = 100 μm. (J) Quantification of GAG area and OARSI scores in I. (K) Quantification of IHC staining in I. Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (A-F and H, J, K).

    Article Snippet: Cells were fixed with ice-cold methanol for 5 min and then blocked with 5% bovine serum albumin for 1 h. Samples were subsequently incubated with primary antibodies against COL2 (1:200, ProteinTech, 28459-1-AP) or MMP13 (1:200, ProteinTech, 18165-1-AP) overnight at 4 °C, followed by incubation with fluorophore-conjugated secondary antibodies for 1 h at room temperature.

    Techniques: Expressing, Isolation, Knock-Out, Immunofluorescence, Staining, Immunohistochemistry, Cell Culture, Comparison

    LCM regulated chondrocyte metabolism through enhancing HSP70 and midkine secretion . (A) ELISA quantification of midkine and HSP70 levels in conditioned medium (CM) collected from human OA chondrocytes treated with 10 nM LCM for 48 h. (B) mRNA levels of COL2 and ACAN in chondrocytes cultured with LCM- CM for 24 h. (C) mRNA levels of MMP13 and ADAMTS5 in human OA chondrocytes treated with 20 ng/mL IL-1β and LCM-CM for 24 h. (D) mRNA expression of COL2 and ACAN in chondrocytes treated with LCM-CM in the presence or absence of anti-HSP70 (αHSP70) antibody for 24 h. (E) mRNA expression of MMP13 and ADAMTS5 in human OA chondrocytes stimulated with 20 ng/ml IL-1β and LCM-CM in the presence or absence of anti-midkine (αMDK) antibody for 24 h. (F, G) Representative immunofluorescence staining of MMP13 (F) and COL2 (G) in C28/I2 chondrocytes with/without IL-1β, LCM-CM, anti-HSP70 antibody and anti-midkine antibody. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (H) Quantification of average optical density (AOD) of MMP13 and COL2 immunofluorescence shown in F and G. (I) Safranin O/Fast Green and IHC staining for MMP13 in human OA cartilage explants cultured with 20 ng/mL IL-1β in the presence or absence of LCM-CM or anti-midkine antibody for 5 days. Scale bar = 100 μm. (J) Safranin O/Fast Green and IHC staining for COL2 in cartilage explants cultured with LCM-CM with or without anti-HSP70 antibody for 5 days. Scale bar = 100 μm. (K) Quantification of GAG area and OARSI scores in I. (L) Quantification of IHC staining in I. (M) Quantification of GAG area and OARSI scores in J. (N) Quantification of IHC staining in J. Data are presented as mean ± SD. Statistical analysis: unpaired two-tailed Student's t-test (A, B) and one-way ANOVA with post hoc comparison (C-E and H, K-N).

    Journal: Bioactive Materials

    Article Title: Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis

    doi: 10.1016/j.bioactmat.2026.02.045

    Figure Lengend Snippet: LCM regulated chondrocyte metabolism through enhancing HSP70 and midkine secretion . (A) ELISA quantification of midkine and HSP70 levels in conditioned medium (CM) collected from human OA chondrocytes treated with 10 nM LCM for 48 h. (B) mRNA levels of COL2 and ACAN in chondrocytes cultured with LCM- CM for 24 h. (C) mRNA levels of MMP13 and ADAMTS5 in human OA chondrocytes treated with 20 ng/mL IL-1β and LCM-CM for 24 h. (D) mRNA expression of COL2 and ACAN in chondrocytes treated with LCM-CM in the presence or absence of anti-HSP70 (αHSP70) antibody for 24 h. (E) mRNA expression of MMP13 and ADAMTS5 in human OA chondrocytes stimulated with 20 ng/ml IL-1β and LCM-CM in the presence or absence of anti-midkine (αMDK) antibody for 24 h. (F, G) Representative immunofluorescence staining of MMP13 (F) and COL2 (G) in C28/I2 chondrocytes with/without IL-1β, LCM-CM, anti-HSP70 antibody and anti-midkine antibody. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (H) Quantification of average optical density (AOD) of MMP13 and COL2 immunofluorescence shown in F and G. (I) Safranin O/Fast Green and IHC staining for MMP13 in human OA cartilage explants cultured with 20 ng/mL IL-1β in the presence or absence of LCM-CM or anti-midkine antibody for 5 days. Scale bar = 100 μm. (J) Safranin O/Fast Green and IHC staining for COL2 in cartilage explants cultured with LCM-CM with or without anti-HSP70 antibody for 5 days. Scale bar = 100 μm. (K) Quantification of GAG area and OARSI scores in I. (L) Quantification of IHC staining in I. (M) Quantification of GAG area and OARSI scores in J. (N) Quantification of IHC staining in J. Data are presented as mean ± SD. Statistical analysis: unpaired two-tailed Student's t-test (A, B) and one-way ANOVA with post hoc comparison (C-E and H, K-N).

    Article Snippet: Cells were fixed with ice-cold methanol for 5 min and then blocked with 5% bovine serum albumin for 1 h. Samples were subsequently incubated with primary antibodies against COL2 (1:200, ProteinTech, 28459-1-AP) or MMP13 (1:200, ProteinTech, 18165-1-AP) overnight at 4 °C, followed by incubation with fluorophore-conjugated secondary antibodies for 1 h at room temperature.

    Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Expressing, Immunofluorescence, Staining, Immunohistochemistry, Two Tailed Test, Comparison

    Systemic administration of LCM attenuates OA progression and pain . (A) Schematic of the experimental outline. Twelve-week-old male WT mice underwent DMM surgery. Beginning 4 weeks post-surgery, mice were treated daily with either CBZ (10 mg/kg) or LCM (1, 10, or 50 mg/kg) by oral gavage for a total duration of 8 weeks. (n = 6). (B) Representative Safranin O/Fast Green stained images of knee joints at 12 weeks post-DMM surgery, systemically treated with CBZ (10 mg/kg/day) or LCM (1, 10, or 50 mg/kg/day) (n = 6). Scale bar = 100 μm. (C) Quantification of OARSI scores, subchondral bone plate (SBP) thickness, osteophyte formation, and synovitis. (D, E) Behavioral outcomes assessed by open-field travel distance (D) and von Frey test (E). (F) Representative IHC staining of Col2, Aggrecan neoepitope, Mmp13, and Adamts5 in joint sections (n = 6). Scale bar = 50 μm ∗Vehicle versus LCM (50 mg/kg/day), # Vehicle versus LCM (10 mg/kg/day),(∗ ,# P < 0.05; ∗∗ ,## P < 0.01). Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (C) and unpaired two-tailed Student's t-test (D, E).

    Journal: Bioactive Materials

    Article Title: Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis

    doi: 10.1016/j.bioactmat.2026.02.045

    Figure Lengend Snippet: Systemic administration of LCM attenuates OA progression and pain . (A) Schematic of the experimental outline. Twelve-week-old male WT mice underwent DMM surgery. Beginning 4 weeks post-surgery, mice were treated daily with either CBZ (10 mg/kg) or LCM (1, 10, or 50 mg/kg) by oral gavage for a total duration of 8 weeks. (n = 6). (B) Representative Safranin O/Fast Green stained images of knee joints at 12 weeks post-DMM surgery, systemically treated with CBZ (10 mg/kg/day) or LCM (1, 10, or 50 mg/kg/day) (n = 6). Scale bar = 100 μm. (C) Quantification of OARSI scores, subchondral bone plate (SBP) thickness, osteophyte formation, and synovitis. (D, E) Behavioral outcomes assessed by open-field travel distance (D) and von Frey test (E). (F) Representative IHC staining of Col2, Aggrecan neoepitope, Mmp13, and Adamts5 in joint sections (n = 6). Scale bar = 50 μm ∗Vehicle versus LCM (50 mg/kg/day), # Vehicle versus LCM (10 mg/kg/day),(∗ ,# P < 0.05; ∗∗ ,## P < 0.01). Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (C) and unpaired two-tailed Student's t-test (D, E).

    Article Snippet: Cells were fixed with ice-cold methanol for 5 min and then blocked with 5% bovine serum albumin for 1 h. Samples were subsequently incubated with primary antibodies against COL2 (1:200, ProteinTech, 28459-1-AP) or MMP13 (1:200, ProteinTech, 18165-1-AP) overnight at 4 °C, followed by incubation with fluorophore-conjugated secondary antibodies for 1 h at room temperature.

    Techniques: Staining, Immunohistochemistry, Comparison, Two Tailed Test

    Intra-articular delivery of LCM protects against OA . (A) Schematic of the experimental outline. Twelve-week-old male WT mice underwent DMM surgery. Beginning 4 weeks post-surgery, mice received intra-articular injections of CBZ (1 mg/kg, three times per week) or LCM (0.1 or 1 mg/kg, three times per week) for a total duration of 8 weeks (n = 6). (B) Representative Safranin O/Fast Green stained sections of knee joints at 12 weeks post-surgery, treated with or without CBZ (1 mg/kg, 3 × /week) or LCM (0.1 or 1 mg/kg, 3 × /week) intra-articularly (n = 6). Scale bar = 100 μm. (C) Quantification of OARSI scores, subchondral bone plate (SBP) thickness, osteophyte formation, and synovitis. (D, E) Behavioral outcomes assessed by open-field travel distance (D) and von Frey test (E). (F) Representative IHC staining of Col2, Aggrecan neoepitope, Mmp13, and Adamts5 in joint sections (n = 6). Scale bar = 50 μm ∗Vehicle versus LCM (1 mg/kg, 3 × /week), # Vehicle versus LCM (0.1 mg/kg, 3 × /week),(∗ ,# P < 0.05). Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (C) and unpaired two-tailed Student's t-test (D, E).

    Journal: Bioactive Materials

    Article Title: Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis

    doi: 10.1016/j.bioactmat.2026.02.045

    Figure Lengend Snippet: Intra-articular delivery of LCM protects against OA . (A) Schematic of the experimental outline. Twelve-week-old male WT mice underwent DMM surgery. Beginning 4 weeks post-surgery, mice received intra-articular injections of CBZ (1 mg/kg, three times per week) or LCM (0.1 or 1 mg/kg, three times per week) for a total duration of 8 weeks (n = 6). (B) Representative Safranin O/Fast Green stained sections of knee joints at 12 weeks post-surgery, treated with or without CBZ (1 mg/kg, 3 × /week) or LCM (0.1 or 1 mg/kg, 3 × /week) intra-articularly (n = 6). Scale bar = 100 μm. (C) Quantification of OARSI scores, subchondral bone plate (SBP) thickness, osteophyte formation, and synovitis. (D, E) Behavioral outcomes assessed by open-field travel distance (D) and von Frey test (E). (F) Representative IHC staining of Col2, Aggrecan neoepitope, Mmp13, and Adamts5 in joint sections (n = 6). Scale bar = 50 μm ∗Vehicle versus LCM (1 mg/kg, 3 × /week), # Vehicle versus LCM (0.1 mg/kg, 3 × /week),(∗ ,# P < 0.05). Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (C) and unpaired two-tailed Student's t-test (D, E).

    Article Snippet: Cells were fixed with ice-cold methanol for 5 min and then blocked with 5% bovine serum albumin for 1 h. Samples were subsequently incubated with primary antibodies against COL2 (1:200, ProteinTech, 28459-1-AP) or MMP13 (1:200, ProteinTech, 18165-1-AP) overnight at 4 °C, followed by incubation with fluorophore-conjugated secondary antibodies for 1 h at room temperature.

    Techniques: Staining, Immunohistochemistry, Comparison, Two Tailed Test

    Hydrogel-mediated delivery of LCM provides prolonged joint retention and therapeutic efficacy . (A) Schematic of the experimental outline. Twelve-week-old male WT mice underwent DMM surgery. Beginning at 4 weeks post-surgery, mice received intra-articular injections of either blank hydrogel or LCM-loaded hydrogel once monthly for 2 months (n = 6). (B) Safranin O/Fast Green–stained sections of knee joints 12 weeks post-DMM surgery, following intra-articular injection of LCM-loaded hydrogel (n = 6). Scale bar = 100 μm. (C) Quantification of OARSI scores, SBP thickness, osteophyte size, and synovitis in each group. (D, E) Behavior test outcomes assessed by open-field movement (D) and von Frey test (E). (F) Representative IHC staining of Col2, Aggrecan neoepitope, Mmp13, and Adamts5 in joint sections (n = 6). Scale bar = 50 μm. (G) Quantification of IHC-positive staining in (F). ∗Vehicle versus LCM-loaded hydrogel, (∗ P < 0.05; ∗∗ P < 0.01, ∗∗∗ P < 0.001). Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (C, G) and unpaired two-tailed Student's t-test (D, E).

    Journal: Bioactive Materials

    Article Title: Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis

    doi: 10.1016/j.bioactmat.2026.02.045

    Figure Lengend Snippet: Hydrogel-mediated delivery of LCM provides prolonged joint retention and therapeutic efficacy . (A) Schematic of the experimental outline. Twelve-week-old male WT mice underwent DMM surgery. Beginning at 4 weeks post-surgery, mice received intra-articular injections of either blank hydrogel or LCM-loaded hydrogel once monthly for 2 months (n = 6). (B) Safranin O/Fast Green–stained sections of knee joints 12 weeks post-DMM surgery, following intra-articular injection of LCM-loaded hydrogel (n = 6). Scale bar = 100 μm. (C) Quantification of OARSI scores, SBP thickness, osteophyte size, and synovitis in each group. (D, E) Behavior test outcomes assessed by open-field movement (D) and von Frey test (E). (F) Representative IHC staining of Col2, Aggrecan neoepitope, Mmp13, and Adamts5 in joint sections (n = 6). Scale bar = 50 μm. (G) Quantification of IHC-positive staining in (F). ∗Vehicle versus LCM-loaded hydrogel, (∗ P < 0.05; ∗∗ P < 0.01, ∗∗∗ P < 0.001). Data are presented as mean ± SD. Statistical analysis: one-way ANOVA with post hoc comparison (C, G) and unpaired two-tailed Student's t-test (D, E).

    Article Snippet: Cells were fixed with ice-cold methanol for 5 min and then blocked with 5% bovine serum albumin for 1 h. Samples were subsequently incubated with primary antibodies against COL2 (1:200, ProteinTech, 28459-1-AP) or MMP13 (1:200, ProteinTech, 18165-1-AP) overnight at 4 °C, followed by incubation with fluorophore-conjugated secondary antibodies for 1 h at room temperature.

    Techniques: Drug discovery, Staining, Injection, Immunohistochemistry, Comparison, Two Tailed Test