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    Structured Review

    Worthington Biochemical ml
    Ml, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ml/ml/custom%40ml%4042853924
    Average 99 stars, based on 1 article reviews
    ml - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Tissue:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Gentle:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Suspension:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Saline:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Analysis:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    FACS:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Single Cell:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Virus Isolation Assay:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Isolation:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Infection:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Adsorption:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Virus:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Flow Cytometry:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Cytometry:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Incubation:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Oligomer Restriction:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30

    Mouse Assay:

    Article Title: Correlative and Live-Cell Imaging of S100B-Promoter-Associated Interstitial Cells from the Cardiac Sinoatrial Node and Valves.
    Article Snippet: NaCl, 5.4 KCl, 0.5 MgCl2, 0.33 NaH2PO4, 5 HEPES, and 5.5 glucose (pH 7.0) for 10 min at 35 degrees C. They were then digested in 3 mL Ca2+-free Tyrode solution containing lyophilized elastase (2.0 mg; Worthington Biochemical, Lakewood, NJ, USA), protease (0.4 mg; Sigma- Aldrich, Burlington, MA, USA), collagenase type II (3 mg; Worthington Biochemical), and bovine serum albumin (3 mg; Sigma) for 30



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    Image Search Results


    Membrane potential and migration velocity of C5a‐stimulated neutrophils in the absence and presence of different K + channel inhibitors. Neutrophils were always stimulated with 6 nmol/L C5a at t = 0 min. K + channel inhibitors were present throughout the entire experiment as indicated. The thin grey lines in panels A, C, E and G represent the mean values of neutrophils isolated from individual mice. The thick black lines illustrate the mean values derived from all individual mice. The mean values plotted in panels B, D, F and H were obtained from the time periods labeled by the shaded areas in panels A, C, E, and G. Rest., Peak and Repol. correspond to t = −3–0 min, maximal depolarization and t = 8–10 min, respectively. (A,B) Control experiments in the absence of K + channel inhibitors ( N = 12 mice; n = 135 cells; n ≥ 5 cells each). (C, D) Inhibition of K Ca 3.1 channels with 10 µmol/L senicapoc ( N = 4 mice; n = 40 cells; n ≥ 9 cells each). (E, F) Inhibition of K V 1.3 channels with Vm24 ( N = 4 mice; n = 42 cells; n ≥ 10 cells each). (G, H) Inhibition of K ir 2.1 channels with 10 µmol/L ML133 ( N = 3 mice; n = 34 cells; n ≥ 10 cells each). Normally distributed data were analyzed with one‐way ANOVA followed by Tukey's multiple comparison.

    Journal: Archiv Der Pharmazie

    Article Title: K + Channels in Membrane Potential Dynamics and Migration of Neutrophil Granulocytes

    doi: 10.1002/ardp.70365

    Figure Lengend Snippet: Membrane potential and migration velocity of C5a‐stimulated neutrophils in the absence and presence of different K + channel inhibitors. Neutrophils were always stimulated with 6 nmol/L C5a at t = 0 min. K + channel inhibitors were present throughout the entire experiment as indicated. The thin grey lines in panels A, C, E and G represent the mean values of neutrophils isolated from individual mice. The thick black lines illustrate the mean values derived from all individual mice. The mean values plotted in panels B, D, F and H were obtained from the time periods labeled by the shaded areas in panels A, C, E, and G. Rest., Peak and Repol. correspond to t = −3–0 min, maximal depolarization and t = 8–10 min, respectively. (A,B) Control experiments in the absence of K + channel inhibitors ( N = 12 mice; n = 135 cells; n ≥ 5 cells each). (C, D) Inhibition of K Ca 3.1 channels with 10 µmol/L senicapoc ( N = 4 mice; n = 40 cells; n ≥ 9 cells each). (E, F) Inhibition of K V 1.3 channels with Vm24 ( N = 4 mice; n = 42 cells; n ≥ 10 cells each). (G, H) Inhibition of K ir 2.1 channels with 10 µmol/L ML133 ( N = 3 mice; n = 34 cells; n ≥ 10 cells each). Normally distributed data were analyzed with one‐way ANOVA followed by Tukey's multiple comparison.

    Article Snippet: When indicated, we added either senicapoc (K Ca 3.1 inhibitor; 10 μM, kindly provided by Christina Kick, Institut für Pharmazeutische und Medizinische Chemie, University Münster), Vm24 (K V 1.3 inhibitor; 10 nM [ ]) or ML133 (K ir 2.1 inhibitor; 10 μM, Tocris Bioscience, UK) to the Ringer's solution.

    Techniques: Membrane, Migration, Isolation, Derivative Assay, Labeling, Control, Inhibition, Comparison

    Summary of key parameters obtained from the mathematical model analysis. The para meters are calculated for the movement into the direction of the C5a gradient (Y coordinate).

    Journal: Archiv Der Pharmazie

    Article Title: K + Channels in Membrane Potential Dynamics and Migration of Neutrophil Granulocytes

    doi: 10.1002/ardp.70365

    Figure Lengend Snippet: Summary of key parameters obtained from the mathematical model analysis. The para meters are calculated for the movement into the direction of the C5a gradient (Y coordinate).

    Article Snippet: When indicated, we added either senicapoc (K Ca 3.1 inhibitor; 10 μM, kindly provided by Christina Kick, Institut für Pharmazeutische und Medizinische Chemie, University Münster), Vm24 (K V 1.3 inhibitor; 10 nM [ ]) or ML133 (K ir 2.1 inhibitor; 10 μM, Tocris Bioscience, UK) to the Ringer's solution.

    Techniques: Analysis