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mptp assay kit  (Beyotime)


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    Beyotime mptp assay kit
    Functional measurements of mitochondrial activity in <t>rescued</t> <t>tenocytes.</t> A ΔΨm was determined by confocal microscopy using JC-1 dye ( n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in <t>mPTP</t> opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI ( n = 3). D Quantitative analysis of ATP using the ATP determination kit ( n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content ( n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 ( n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). * P < 0.05, ** P < 0.01, *** P < 0.001
    Mptp Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mitochondrial+function+analyses/pmc10134653-107-8-11?v=Beyotime
    Average 99 stars, based on 328 article reviews
    mptp assay kit - by Bioz Stars, 2026-08
    99/100 stars

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    1) Product Images from "Mitochondrial transfer from bone mesenchymal stem cells protects against tendinopathy both in vitro and in vivo"

    Article Title: Mitochondrial transfer from bone mesenchymal stem cells protects against tendinopathy both in vitro and in vivo

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-023-03329-0

    Functional measurements of mitochondrial activity in rescued tenocytes. A ΔΨm was determined by confocal microscopy using JC-1 dye ( n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in mPTP opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI ( n = 3). D Quantitative analysis of ATP using the ATP determination kit ( n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content ( n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 ( n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). * P < 0.05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: Functional measurements of mitochondrial activity in rescued tenocytes. A ΔΨm was determined by confocal microscopy using JC-1 dye ( n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in mPTP opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI ( n = 3). D Quantitative analysis of ATP using the ATP determination kit ( n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content ( n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 ( n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). * P < 0.05, ** P < 0.01, *** P < 0.001

    Techniques Used: Functional Assay, Activity Assay, Confocal Microscopy, Fluorescence, Staining, Western Blot, Expressing



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