Review



aquapel-treated microfluidic device  (FlowJEM Inc)

 
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    • 90
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    Structured Review

    FlowJEM Inc aquapel-treated microfluidic device
    ( A ) Workflow showing DAPI-stained nuclei pre- and post-FACS/filtration that underwent <t>microfluidic</t> partitioning and library preparation in the 10X genomics platform followed by sequencing using an Illumina HiSeq 4000. ( B ) tSNE-plot showing 14 clusters from ~6000 adipocytes derived from iWAT of mice exposed to cold for 24 hr. Each colored dot is an adipocyte assigned to a cluster based on transcriptomic signature. ( C ) Normalized expression values of the top two adipocyte subtype-specific cluster genes from ( B ) plotted as violin plots with clusters as rows and genes as columns. ( D ) tSNE-plot showing cluster-specific expression of selected marker genes from ( C ). ( E ) Normalized expression values of indicated genes in subtype-specific clusters plotted as violin plots with clusters as rows and genes as columns. Black arrow is pointing toward metabolically active Type nine adipocyte cluster and enriched gene.
    Aquapel Treated Microfluidic Device, supplied by FlowJEM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miscript+microfluidics+preamp+kit/pmc06837845-9-5-8?v=FlowJEM+Inc
    Average 90 stars, based on 1 article reviews
    aquapel-treated microfluidic device - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes"

    Article Title: Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes

    Journal: eLife

    doi: 10.7554/eLife.49501

    ( A ) Workflow showing DAPI-stained nuclei pre- and post-FACS/filtration that underwent microfluidic partitioning and library preparation in the 10X genomics platform followed by sequencing using an Illumina HiSeq 4000. ( B ) tSNE-plot showing 14 clusters from ~6000 adipocytes derived from iWAT of mice exposed to cold for 24 hr. Each colored dot is an adipocyte assigned to a cluster based on transcriptomic signature. ( C ) Normalized expression values of the top two adipocyte subtype-specific cluster genes from ( B ) plotted as violin plots with clusters as rows and genes as columns. ( D ) tSNE-plot showing cluster-specific expression of selected marker genes from ( C ). ( E ) Normalized expression values of indicated genes in subtype-specific clusters plotted as violin plots with clusters as rows and genes as columns. Black arrow is pointing toward metabolically active Type nine adipocyte cluster and enriched gene.
    Figure Legend Snippet: ( A ) Workflow showing DAPI-stained nuclei pre- and post-FACS/filtration that underwent microfluidic partitioning and library preparation in the 10X genomics platform followed by sequencing using an Illumina HiSeq 4000. ( B ) tSNE-plot showing 14 clusters from ~6000 adipocytes derived from iWAT of mice exposed to cold for 24 hr. Each colored dot is an adipocyte assigned to a cluster based on transcriptomic signature. ( C ) Normalized expression values of the top two adipocyte subtype-specific cluster genes from ( B ) plotted as violin plots with clusters as rows and genes as columns. ( D ) tSNE-plot showing cluster-specific expression of selected marker genes from ( C ). ( E ) Normalized expression values of indicated genes in subtype-specific clusters plotted as violin plots with clusters as rows and genes as columns. Black arrow is pointing toward metabolically active Type nine adipocyte cluster and enriched gene.

    Techniques Used: Staining, Filtration, Sequencing, Derivative Assay, Expressing, Marker, Metabolic Labelling


    Figure Legend Snippet:

    Techniques Used: Isolation, Multiplex Assay, Lysis, Software



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