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Arraystar inc mirna expression profiling microarray
Comparison of the expression profiles of miRNAs(A-C) and mRNAs (D-F) between HbH-CS patients and healthy controls. (A) Scatter plot showing the distribution of <t>miRNA</t> expression. (B) Volcano plot showing the differential expression of miRNAs. (C) The clustering heatmap showed differentially expressed miRNAs between patients with HbH-CS patients and healthy controls. (D) Scatter plot showing the distribution of mRNA expression. (E) Volcano plot showing the differential expression of mRNAs. (F) The clustering heatmap showed differentially expressed mRNAs between patients with HbH-CS patients and healthy controls.
Mirna Expression Profiling Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease"

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease

Journal: Annals of Medicine

doi: 10.1080/07853890.2025.2530690

Comparison of the expression profiles of miRNAs(A-C) and mRNAs (D-F) between HbH-CS patients and healthy controls. (A) Scatter plot showing the distribution of miRNA expression. (B) Volcano plot showing the differential expression of miRNAs. (C) The clustering heatmap showed differentially expressed miRNAs between patients with HbH-CS patients and healthy controls. (D) Scatter plot showing the distribution of mRNA expression. (E) Volcano plot showing the differential expression of mRNAs. (F) The clustering heatmap showed differentially expressed mRNAs between patients with HbH-CS patients and healthy controls.
Figure Legend Snippet: Comparison of the expression profiles of miRNAs(A-C) and mRNAs (D-F) between HbH-CS patients and healthy controls. (A) Scatter plot showing the distribution of miRNA expression. (B) Volcano plot showing the differential expression of miRNAs. (C) The clustering heatmap showed differentially expressed miRNAs between patients with HbH-CS patients and healthy controls. (D) Scatter plot showing the distribution of mRNA expression. (E) Volcano plot showing the differential expression of mRNAs. (F) The clustering heatmap showed differentially expressed mRNAs between patients with HbH-CS patients and healthy controls.

Techniques Used: Comparison, Expressing, Quantitative Proteomics

Bioinformatics analysis. (A) Venn diagram showed stratified operations of miRNAs from the original data and online database. (B) Intersection plot of mRNAs from our previous ArrayStar human mRNA array and miR-223-3p target gene predicted by online database. (C) Prediction plot of miR-223-3p target gene. Yellow circled node, miR-223-3p; blue rectangle type node, mRNA. (D, E) The qRT-PCR was performed to detect the relative expression levels of miR-223-3p (D) and TGFBR3 (E) in the samples from healthy normal subjects and HbH-CS patients. Normal group, n = 17; HbH-CS group, n = 17, mean ± SEM, ** p < 0.01, *** p < 0.001.
Figure Legend Snippet: Bioinformatics analysis. (A) Venn diagram showed stratified operations of miRNAs from the original data and online database. (B) Intersection plot of mRNAs from our previous ArrayStar human mRNA array and miR-223-3p target gene predicted by online database. (C) Prediction plot of miR-223-3p target gene. Yellow circled node, miR-223-3p; blue rectangle type node, mRNA. (D, E) The qRT-PCR was performed to detect the relative expression levels of miR-223-3p (D) and TGFBR3 (E) in the samples from healthy normal subjects and HbH-CS patients. Normal group, n = 17; HbH-CS group, n = 17, mean ± SEM, ** p < 0.01, *** p < 0.001.

Techniques Used: Quantitative RT-PCR, Expressing

Related Articles

Synthesized:

Article Title: Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
Article Snippet: Total RNA was collected using TRIzol Reagent and was purified using the RNeasy Mini Kit (Qiagen, USA). .. Then, the synthesized cDNA was hybridized using miRNA Expression Microarray (Arraystar, USA). miRNAs with differential expression were screened using the GeneSpring GX v12.1 software package (Agilent Technologies, USA), with P<0.05 and |Log FC| ≥2 set as the screening parameters. ..

Article Title: [RETRACTED ARTICLE] Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
Article Snippet: Finally, the samples were treated with 400 mL 1X binding buffer, and the apoptosis of cells was determined using the Cell-Quest software (Becton Dickinson, USA) and a flow cytometer (FACSCalibur, Becton Dickinson). miRNA microarray analysis Total RNA was collected using TRIzol Reagent and was purified using the RNeasy Mini Kit (Qiagen, USA). .. Then, the synthesized cDNA was hybridized using miRNA Expression Microarray (Arraystar, USA). miRNAs with differential expression were screened using the GeneSpring GX v12.1 software package (Agilent Technologies, USA), with Po0.05 and |Log FC| X2 set as the screening parameters. ..

Expressing:

Article Title: Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
Article Snippet: Total RNA was collected using TRIzol Reagent and was purified using the RNeasy Mini Kit (Qiagen, USA). .. Then, the synthesized cDNA was hybridized using miRNA Expression Microarray (Arraystar, USA). miRNAs with differential expression were screened using the GeneSpring GX v12.1 software package (Agilent Technologies, USA), with P<0.05 and |Log FC| ≥2 set as the screening parameters. ..

Article Title: [RETRACTED ARTICLE] Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
Article Snippet: Finally, the samples were treated with 400 mL 1X binding buffer, and the apoptosis of cells was determined using the Cell-Quest software (Becton Dickinson, USA) and a flow cytometer (FACSCalibur, Becton Dickinson). miRNA microarray analysis Total RNA was collected using TRIzol Reagent and was purified using the RNeasy Mini Kit (Qiagen, USA). .. Then, the synthesized cDNA was hybridized using miRNA Expression Microarray (Arraystar, USA). miRNAs with differential expression were screened using the GeneSpring GX v12.1 software package (Agilent Technologies, USA), with Po0.05 and |Log FC| X2 set as the screening parameters. ..

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease.
Article Snippet: .. Analysis of miRNA expression and hematology-related genes MiRNAs associated with hematopoietic cell lineage, apoptosis, and cell cycle were searched in the database, which were stratified by Arraystar miRNA expression profiling microarray data filtered according to p < 0.05 and log FC >1.5. .. Verification of the differentially expressed miRNA and its related mRNA The differentially expressed miRNAs were verified by using quantitative real-time PCR (qRT-PCR). cDNA was synthesized from total RNA by using the PrimeScript RT Enzyme Mix I kit (Takara Bio, Shiga, Japan) and the RT kit for fluorescence quantification of miRNA (Takara Bio, Shiga, Japan), respectively.

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease
Article Snippet: .. MiRNAs associated with hematopoietic cell lineage, apoptosis, and cell cycle were searched in the database, which were stratified by Arraystar miRNA expression profiling microarray data filtered according to p < 0.05 and log FC >1.5. .. The differentially expressed miRNAs were verified by using quantitative real-time PCR (qRT-PCR). cDNA was synthesized from total RNA by using the PrimeScript RT Enzyme Mix I kit (Takara Bio, Shiga, Japan) and the RT kit for fluorescence quantification of miRNA (Takara Bio, Shiga, Japan), respectively.

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease
Article Snippet: .. The Arraystar miRNA expression profile microarray raw data was screened using P -value <0.05 and FC >1.5 as criteria. ..

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease.
Article Snippet: .. Bioinformatics analysis and target gene prediction The Arraystar miRNA expression profile microarray raw data was screened using P-value <0.05 and FC >1.5 as criteria. ..

Article Title: Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells.
Article Snippet: .. First, Arraystar Human LncRNA Expression Array V4.0 and the Whole Human miRNA microarray were used to detect differentially expressed lncRNAs, miRNAs, and mRNAs. ..

Microarray:

Article Title: Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
Article Snippet: Total RNA was collected using TRIzol Reagent and was purified using the RNeasy Mini Kit (Qiagen, USA). .. Then, the synthesized cDNA was hybridized using miRNA Expression Microarray (Arraystar, USA). miRNAs with differential expression were screened using the GeneSpring GX v12.1 software package (Agilent Technologies, USA), with P<0.05 and |Log FC| ≥2 set as the screening parameters. ..

Article Title: [RETRACTED ARTICLE] Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
Article Snippet: Finally, the samples were treated with 400 mL 1X binding buffer, and the apoptosis of cells was determined using the Cell-Quest software (Becton Dickinson, USA) and a flow cytometer (FACSCalibur, Becton Dickinson). miRNA microarray analysis Total RNA was collected using TRIzol Reagent and was purified using the RNeasy Mini Kit (Qiagen, USA). .. Then, the synthesized cDNA was hybridized using miRNA Expression Microarray (Arraystar, USA). miRNAs with differential expression were screened using the GeneSpring GX v12.1 software package (Agilent Technologies, USA), with Po0.05 and |Log FC| X2 set as the screening parameters. ..

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease.
Article Snippet: .. Analysis of miRNA expression and hematology-related genes MiRNAs associated with hematopoietic cell lineage, apoptosis, and cell cycle were searched in the database, which were stratified by Arraystar miRNA expression profiling microarray data filtered according to p < 0.05 and log FC >1.5. .. Verification of the differentially expressed miRNA and its related mRNA The differentially expressed miRNAs were verified by using quantitative real-time PCR (qRT-PCR). cDNA was synthesized from total RNA by using the PrimeScript RT Enzyme Mix I kit (Takara Bio, Shiga, Japan) and the RT kit for fluorescence quantification of miRNA (Takara Bio, Shiga, Japan), respectively.

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease
Article Snippet: .. MiRNAs associated with hematopoietic cell lineage, apoptosis, and cell cycle were searched in the database, which were stratified by Arraystar miRNA expression profiling microarray data filtered according to p < 0.05 and log FC >1.5. .. The differentially expressed miRNAs were verified by using quantitative real-time PCR (qRT-PCR). cDNA was synthesized from total RNA by using the PrimeScript RT Enzyme Mix I kit (Takara Bio, Shiga, Japan) and the RT kit for fluorescence quantification of miRNA (Takara Bio, Shiga, Japan), respectively.

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease
Article Snippet: .. The Arraystar miRNA expression profile microarray raw data was screened using P -value <0.05 and FC >1.5 as criteria. ..

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease.
Article Snippet: .. Bioinformatics analysis and target gene prediction The Arraystar miRNA expression profile microarray raw data was screened using P-value <0.05 and FC >1.5 as criteria. ..

Article Title: Hypoxia associated lncRNA HYPAL promotes proliferation of gastric cancer as ceRNA by sponging miR-431-5p to upregulate CDK14.
Article Snippet: Gastric cancer (GC) is a common malignant solid tumor that is characterized by high hypoxia.. The transcription of genes associated with hypoxia affects tumor occurrence and development.. Long non-coding RNAs (lncRNAs) have been reported to play important roles in cancer development.

Article Title: Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells.
Article Snippet: .. First, Arraystar Human LncRNA Expression Array V4.0 and the Whole Human miRNA microarray were used to detect differentially expressed lncRNAs, miRNAs, and mRNAs. ..

Quantitative Proteomics:

Article Title: Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
Article Snippet: Total RNA was collected using TRIzol Reagent and was purified using the RNeasy Mini Kit (Qiagen, USA). .. Then, the synthesized cDNA was hybridized using miRNA Expression Microarray (Arraystar, USA). miRNAs with differential expression were screened using the GeneSpring GX v12.1 software package (Agilent Technologies, USA), with P<0.05 and |Log FC| ≥2 set as the screening parameters. ..

Article Title: [RETRACTED ARTICLE] Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
Article Snippet: Finally, the samples were treated with 400 mL 1X binding buffer, and the apoptosis of cells was determined using the Cell-Quest software (Becton Dickinson, USA) and a flow cytometer (FACSCalibur, Becton Dickinson). miRNA microarray analysis Total RNA was collected using TRIzol Reagent and was purified using the RNeasy Mini Kit (Qiagen, USA). .. Then, the synthesized cDNA was hybridized using miRNA Expression Microarray (Arraystar, USA). miRNAs with differential expression were screened using the GeneSpring GX v12.1 software package (Agilent Technologies, USA), with Po0.05 and |Log FC| X2 set as the screening parameters. ..

Software:

Article Title: Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
Article Snippet: Total RNA was collected using TRIzol Reagent and was purified using the RNeasy Mini Kit (Qiagen, USA). .. Then, the synthesized cDNA was hybridized using miRNA Expression Microarray (Arraystar, USA). miRNAs with differential expression were screened using the GeneSpring GX v12.1 software package (Agilent Technologies, USA), with P<0.05 and |Log FC| ≥2 set as the screening parameters. ..

Article Title: [RETRACTED ARTICLE] Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation
Article Snippet: Finally, the samples were treated with 400 mL 1X binding buffer, and the apoptosis of cells was determined using the Cell-Quest software (Becton Dickinson, USA) and a flow cytometer (FACSCalibur, Becton Dickinson). miRNA microarray analysis Total RNA was collected using TRIzol Reagent and was purified using the RNeasy Mini Kit (Qiagen, USA). .. Then, the synthesized cDNA was hybridized using miRNA Expression Microarray (Arraystar, USA). miRNAs with differential expression were screened using the GeneSpring GX v12.1 software package (Agilent Technologies, USA), with Po0.05 and |Log FC| X2 set as the screening parameters. ..

Significance Assay:

Article Title: MiR-223-3p regulates erythropoiesis by targeting TGFBR3/Smad signaling pathway in hemoglobin H-Constant Spring disease.
Article Snippet: .. Bioinformatics analysis and target gene prediction The Arraystar miRNA expression profile microarray raw data was screened using P-value <0.05 and FC >1.5 as criteria. ..



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Image Search Results


Study design flowchart. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure. DEcircRNAs, differentially expressed circRNAs; DEmiRNAs, differentially expressed miRNAs; DEmRNAs, differentially expressed mRNAs

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: Study design flowchart. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure. DEcircRNAs, differentially expressed circRNAs; DEmiRNAs, differentially expressed miRNAs; DEmRNAs, differentially expressed mRNAs

Article Snippet: CircRNA, miRNA and mRNA microarray expression profile datasets were selected from the National Center of Biotechnology Information Gene Expression Omnibus (NCBI) GEO ( https://www.ncbi.nlm.nih.gov/geo/ ).

Techniques:

Boxplots, volcano plots and Venn diagram of mRNAs for GSE103465. (A) Boxplot of GSE103465. (B) Volcano plots of DEmRNAs based on GSE103465. (C) Venn diagram of mRNAs for GeneCards and GSE103465, where the intersection section is predicted DEmRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: Boxplots, volcano plots and Venn diagram of mRNAs for GSE103465. (A) Boxplot of GSE103465. (B) Volcano plots of DEmRNAs based on GSE103465. (C) Venn diagram of mRNAs for GeneCards and GSE103465, where the intersection section is predicted DEmRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Article Snippet: CircRNA, miRNA and mRNA microarray expression profile datasets were selected from the National Center of Biotechnology Information Gene Expression Omnibus (NCBI) GEO ( https://www.ncbi.nlm.nih.gov/geo/ ).

Techniques:

Boxplots, volcano plots and Venn diagram of miRNAs for GSE121219. (A) Boxplot of GSE121219. (B) Volcano plots of DEmiRNAs based on GSE121219. (C) Venn diagram of miRNAs for miRDIP and GSE121219, where the intersection section is predicted DEmiRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: Boxplots, volcano plots and Venn diagram of miRNAs for GSE121219. (A) Boxplot of GSE121219. (B) Volcano plots of DEmiRNAs based on GSE121219. (C) Venn diagram of miRNAs for miRDIP and GSE121219, where the intersection section is predicted DEmiRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Article Snippet: CircRNA, miRNA and mRNA microarray expression profile datasets were selected from the National Center of Biotechnology Information Gene Expression Omnibus (NCBI) GEO ( https://www.ncbi.nlm.nih.gov/geo/ ).

Techniques:

Boxplots, volcano plots and Venn diagram of circRNAs for GSE147442. (A) Boxplot of GSE147442 after standardization. (B) Volcano plots of DEcircRNAs based on GSE147442. (C) Venn diagram of miRNAs for starBase 2.0 and GSE147442, where the intersection section is predicted DEcircRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: Boxplots, volcano plots and Venn diagram of circRNAs for GSE147442. (A) Boxplot of GSE147442 after standardization. (B) Volcano plots of DEcircRNAs based on GSE147442. (C) Venn diagram of miRNAs for starBase 2.0 and GSE147442, where the intersection section is predicted DEcircRNAs asscociated with angiogenesis in RIF. circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Article Snippet: CircRNA, miRNA and mRNA microarray expression profile datasets were selected from the National Center of Biotechnology Information Gene Expression Omnibus (NCBI) GEO ( https://www.ncbi.nlm.nih.gov/geo/ ).

Techniques:

Figure 5 CircRNA-miRNA-mRNA regulatory network, which consists of 8 DEcircRNAs, 10 DEmiRNAs and 45 DERNAs. DEcircRNA, differentially expressed circular RNA; DEmiRNA, differentially expressed micro RNA; DERNA, differentially expressed RNA. Arrow-shape nodes: circRNAs, triangle nodes: miRNAs, ellipse-shaped nodes: mRNAs

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: Figure 5 CircRNA-miRNA-mRNA regulatory network, which consists of 8 DEcircRNAs, 10 DEmiRNAs and 45 DERNAs. DEcircRNA, differentially expressed circular RNA; DEmiRNA, differentially expressed micro RNA; DERNA, differentially expressed RNA. Arrow-shape nodes: circRNAs, triangle nodes: miRNAs, ellipse-shaped nodes: mRNAs

Article Snippet: CircRNA, miRNA and mRNA microarray expression profile datasets were selected from the National Center of Biotechnology Information Gene Expression Omnibus (NCBI) GEO ( https://www.ncbi.nlm.nih.gov/geo/ ).

Techniques:

A PPI network and circRNA–miRNA–hub gene regulatory subnetwork. (A) A PPI network of the fifty-five target genes associated with angiogenesis in RIF. (B) Six hub genes extracted by cytoHubba plug-in. (C) CircRNA–miRNA–hub gene regulatory subnetwork, consisting of 3 circRNAs, 3 miRNAs, and 3 mRNAs. PPI, protein–protein interaction; circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Journal: BMC Medical Genomics

Article Title: Comprehensive analysis of circRNA-miRNA-mRNA network related to angiogenesis in recurrent implantation failure

doi: 10.1186/s12920-024-01944-1

Figure Lengend Snippet: A PPI network and circRNA–miRNA–hub gene regulatory subnetwork. (A) A PPI network of the fifty-five target genes associated with angiogenesis in RIF. (B) Six hub genes extracted by cytoHubba plug-in. (C) CircRNA–miRNA–hub gene regulatory subnetwork, consisting of 3 circRNAs, 3 miRNAs, and 3 mRNAs. PPI, protein–protein interaction; circRNA, circular RNA; miRNA, microRNA; RIF, recurrent implantation failure

Article Snippet: CircRNA, miRNA and mRNA microarray expression profile datasets were selected from the National Center of Biotechnology Information Gene Expression Omnibus (NCBI) GEO ( https://www.ncbi.nlm.nih.gov/geo/ ).

Techniques: