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CapitalBio Corporation mirna microarray chip

Mirna Microarray Chip, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mirna+microarray+chip+screening/mirna+microarray/pmc05829740-64-12-18
Average 90 stars, based on 1 article reviews

mirna microarray chip - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Nuclear miR-122 directly regulates the biogenesis of cell survival oncomiR miR-21 at the posttranscriptional level"

Article Title: Nuclear miR-122 directly regulates the biogenesis of cell survival oncomiR miR-21 at the posttranscriptional level

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkx1254

Figure Legend Snippet: MiR-122 localizes in the nucleus of liver cells. ( A ) Nuclear miRNA expression profile in mouse liver cells via detecting by miRNA microarray. The most 50 enriched nuclear miRNAs were plotted in the figure. ( B ) MiR-122, miR-29b and miR-29a expression levels in mouse hepatocytes and nuclei (upper panel), human Huh-7 cells and nuclei (lower panel) by using TaqMan probe-based RT-qPCR assay. ( C ) Nuclear localization of synthetic Cy3-labeled miR-122, miR-29b and miR-29a mimics in Huh-7 cells. ( D ) 3-D re-constituted images of Z-stacks of Cy3-labeled miR-122, miR-29b and miR-29a mimics in Huh-7 cells. White arrowheads indicated the merged pink colored spots. ( E ) Distribution of miR-122 in Huh-7 cells by FISH using Dig-labeled miRCURY LNA™ microRNA Detection Probes. All images were obtained with the confocal microscope. The nuclear expression of U6 was visualized using the miRCURY LNA™ U6 probe as a positive control. DAPI was dyed with blue and probes were labeled with red by anti-Dig-rhodamine. Bars, 20 μm.

Techniques Used: Expressing, Microarray, Quantitative RT-PCR, Labeling, Microscopy, Positive Control

Figure Legend Snippet: Nuclear miR-122 inhibits miR-21 biogenesis. ( A ) TaqMan Low Density Array screening for target miRNAs of miR-122. Huh-7 cells were transfected with miR-122 mimic or control oligonucleotide and then harvested 24 h after transfection. The miRNA expression profile was sorted using a hierarchical clustering method (Cluster 3.0 and Java TreeView). Twenty eight most significantly changed miRNAs were shown in the cluster. ( B ) RT-qPCR validation of decreased miRNAs screened by Low Density Array following ectopic expression of miR-122 mimic or control. ( C ) Relative pri-miR-21, pre-miR-21 and miR-21 expression levels in Huh-7 cells after miR-122 overexpression or depletion. ( D ) RT-qPCR analysis of expression levels of miR-122 in 16 paired HCC and ANCT tissue samples. ( E ) RT-qPCR analysis of miR-21 level in 16 paired HCC and ANCT samples. ( F ) Pearson's correlation scatter plot of the levels of miR-122 and miR-21 in 16 paired HCC tissues. The results are presented as the mean ± SD ( N = 3) of three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.

Techniques Used: TLDA Assay, Transfection, Control, Expressing, Quantitative RT-PCR, Biomarker Discovery, Over Expression

Nuclear miR-122 directly binds to a cognate element on pri-miR-21 transcript. ( A ) Putative binding site for miR-122 on human pri-miR-21 as predicted using RNAhybrid. As shown in the schematic diagram, a near perfect complementary site (red rectangle) for miR-122 (black rectangle) was located 13 bp upstream of the pre-miR-21 gene loci. mfe: minimum free energy. ( B ) Schematic representations of modified GFP expression plasmid. MiR-122 binding sequences (Binding-WT) and mutant sequences (Binding-MUT) were inserted into the 3′-UTR of GFP in GFP expression plasmid. ( C ) HEK-293T cells were co-transfected with the modified vector and miR-122 mimic. After 48 h, fluorescence microscopy was used to detect GFP expression. Scale bar: 1.0 mm. ( D ) The GFP mRNA expression level was detected by RT-qPCR. NS: no significance. ( E ) Schematic illustration of pri-miR-21 pull-down strategy. ( F and G ) Levels of pri-miR-21 (F) or miR-122 and other candidate miRNAs (G) in pull-down products which were co-precipitated by anti-pri-miR-21 probe or random probe. Data are presented as mean ± SD ( N = 3) of three independent experiments. ** P < 0.01; *** P < 0.001.

Figure Legend Snippet: Nuclear miR-122 directly binds to a cognate element on pri-miR-21 transcript. ( A ) Putative binding site for miR-122 on human pri-miR-21 as predicted using RNAhybrid. As shown in the schematic diagram, a near perfect complementary site (red rectangle) for miR-122 (black rectangle) was located 13 bp upstream of the pre-miR-21 gene loci. mfe: minimum free energy. ( B ) Schematic representations of modified GFP expression plasmid. MiR-122 binding sequences (Binding-WT) and mutant sequences (Binding-MUT) were inserted into the 3′-UTR of GFP in GFP expression plasmid. ( C ) HEK-293T cells were co-transfected with the modified vector and miR-122 mimic. After 48 h, fluorescence microscopy was used to detect GFP expression. Scale bar: 1.0 mm. ( D ) The GFP mRNA expression level was detected by RT-qPCR. NS: no significance. ( E ) Schematic illustration of pri-miR-21 pull-down strategy. ( F and G ) Levels of pri-miR-21 (F) or miR-122 and other candidate miRNAs (G) in pull-down products which were co-precipitated by anti-pri-miR-21 probe or random probe. Data are presented as mean ± SD ( N = 3) of three independent experiments. ** P < 0.01; *** P < 0.001.

Techniques Used: Binding Assay, Modification, Expressing, Plasmid Preparation, Mutagenesis, Transfection, Fluorescence, Microscopy, Quantitative RT-PCR

MiR-122 blocks the primary miR-21 processing by in vitro pri-miRNA processing assay. ( A ) Schematic illustration of pri-miR-21 in vitro processing assay strategy. ( B ) For immunoprecipitation (IP) assays, Huh-7 cell lysates were incubated with anti-Drosha antibody and IgG control antibody, The IP-product was then detected by western blotting (WB) using anti-Drosha and anti-DGCR8 antibody. ( C ) Northern blotting analysis of the miR-122 blocking in vitro processing of the pri-miR-21-WT and pri-miR-21-MUT. The pri-miR-21-WT and pri-miR-21-MUT transcripts were incubation of synthetic mature single strand miR-122, respectively, and then cleaved by Drosha-complex in vitro . The in vitro processing products were analyzed by northern blot. ( D ) RT-qPCR validation of pre-miR-21 level in processing products. ( E ) The pri-miR-21-WT and pri-miR-21-MUT transcripts were incubation of synthetic mature single strand miR-122, respectively, and then cleaved by nuclear extracts. The in vitro processing products were analyzed by northern blot. ( F ) An unrelated pri-miR-150 was incubation of synthetic mature single strand miR-122 and then cleaved by nuclear extracts. The in vitro processing products were analyzed by northern blot. The results are presented as the mean ± SD ( N = 3) of three independent experiments. ** P < 0.01.

Figure Legend Snippet: MiR-122 blocks the primary miR-21 processing by in vitro pri-miRNA processing assay. ( A ) Schematic illustration of pri-miR-21 in vitro processing assay strategy. ( B ) For immunoprecipitation (IP) assays, Huh-7 cell lysates were incubated with anti-Drosha antibody and IgG control antibody, The IP-product was then detected by western blotting (WB) using anti-Drosha and anti-DGCR8 antibody. ( C ) Northern blotting analysis of the miR-122 blocking in vitro processing of the pri-miR-21-WT and pri-miR-21-MUT. The pri-miR-21-WT and pri-miR-21-MUT transcripts were incubation of synthetic mature single strand miR-122, respectively, and then cleaved by Drosha-complex in vitro . The in vitro processing products were analyzed by northern blot. ( D ) RT-qPCR validation of pre-miR-21 level in processing products. ( E ) The pri-miR-21-WT and pri-miR-21-MUT transcripts were incubation of synthetic mature single strand miR-122, respectively, and then cleaved by nuclear extracts. The in vitro processing products were analyzed by northern blot. ( F ) An unrelated pri-miR-150 was incubation of synthetic mature single strand miR-122 and then cleaved by nuclear extracts. The in vitro processing products were analyzed by northern blot. The results are presented as the mean ± SD ( N = 3) of three independent experiments. ** P < 0.01.

Techniques Used: In Vitro, Immunoprecipitation, Incubation, Control, Western Blot, Northern Blot, Blocking Assay, Quantitative RT-PCR, Biomarker Discovery

other:

Article Title: Regulatory effects of miR-28 on osteogenic differentiation of human bone marrow mesenchymal stem cells
Article Snippet: TRIzol solution was from Invitrogen (USA), miRNA Isolation Kits were from Roche (Switzerland), and miRNA microarray V2.0 was purchased from Beijing CapitalBio Corporation (China; 576 miRNAs labeled in human, 238 in rats, and 358 in mice).

Expressing:

Article Title: Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps
Article Snippet: .. MiRNA expression profile microarrays of these specimens were performed by CapitalBio. ..

Control:

Article Title: Oncogenic miR-20b-5p contributes to malignant behaviors of breast cancer stem cells by bidirectionally regulating CCND1 and E2F1.
Article Snippet: CD44+CD24−/low BCSCs were isolated from MCF-7 and T47D cells by staining with CD44-APC, CD24-PE and ESA-FITC (BD Pharmingen, USA) antibodies via FACS as described in our previous research [8, 20]. miRNA profile and miRNA network Both human miRNA microarray fabrication and hybridization were performed as described previously [8]. .. The miRNA profiles of both BCSCs and control MCF-7 breast cancer cells were obtained from CapitalBio Corporation (Beijing, China). ..

Purification:

Article Title: MiR-672-5p-Mediated Upregulation of REEP6 in Spinal Dorsal Horn Participates in Bortezomib-Induced Neuropathic Pain in Rats.
Article Snippet: Evidence shows that miRNAs are deeply involved in nervous system diseases, but whether miRNAs contribute to the bortezomib (BTZ)-induced neuropathic pain remains unclear.. We aimed to investigate whether miRNAs contribute to bortezomib (BTZ)-induced neuropathic pain and explore the related downstream cascades.. The level of miRNAs in the spinal dorsal horn was explored using miRNA microarray and PCR.

Labeling:

Hybridization:

Microarray:

Article Title: MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell.
Article Snippet: The total RNAs were isolated from 6 CC tissues and 6 PC tissues in according to the protocol of TRIzol reagent (15,596,026, Invitrogen, USA) and purified using mirVanaTM miRNA Isolation Kit (AM1561, Life Technologies, USA). .. Then, the differential expression of RNA was identified using miRNA microarray by CapitalBio Corporation (CapitalBio, China). ..

Article Title: Retracted: Transcription activation of microRNA-25 by PEA3 augments progression of gastric cancer through suppressing SIK1.
Article Snippet: .. A miRNA microarray analysis was performed by CapitalBio (Capital BioCorp, Beijing China) using tumor and the paired normal tissue samples from three patients. .. Total RNA from tissues was collected by TRIzol Reagent (Thermo Fisher Scientific Inc., Waltham, MA, USA).

Article Title: miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis
Article Snippet: .. Then, the microarray analysis of miRNAs was performed by CapitalBio Corporation following the protocol as previously described ( ). ..

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