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qiaseq mirna quantification data analysis software  (Qiagen)


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    Qiagen qiaseq mirna quantification data analysis software
    Qiaseq Mirna Quantification Data Analysis Software, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirna+data+analysis+software/qiaseq+mirna+quantification+tool/pmc10046356-61-7-14
    Average 90 stars, based on 1 article reviews
    qiaseq mirna quantification data analysis software - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Gastroesophageal circulating tumor cell crosstalk with peripheral immune system guides CTC survival and proliferation.
    Article Snippet: Library pools were diluted to 1.5 pM and sequenced using NextSeq 500/550 High Output Kit v2.5 75 cycles flow cell (Cat. N° 20024906, Illumina, San Diego, CA, USA) on NextSeq 500 platform (Illumina). .. Sequencing data analysis: raw data (FASTQ) were analyzed according to the QIAseq miRNA Primary Quantification pipeline using the GeneGlobe Data Analysis Center (available at https:// geneglobe.qiagen.com/us/analyze Qiagen, Hilden Germany). ..

    Article Title: Gastroesophageal circulating tumor cell crosstalk with peripheral immune system guides CTC survival and proliferation
    Article Snippet: Library pools were diluted to 1.5 pM and sequenced using NextSeq 500/550 High Output Kit v2.5 75 cycles flow cell (Cat. N° 20024906, Illumina, San Diego, CA, USA) on NextSeq 500 platform (Illumina). .. Sequencing data analysis: raw data (FASTQ) were analyzed according to the QIAseq miRNA Primary Quantification pipeline using the GeneGlobe Data Analysis Center (available at https://geneglobe.qiagen.com/us/analyze Qiagen, Hilden Germany). ..

    Control:

    Article Title: miRNA signatures underlie chemoresistance in the gemcitabine-resistant pancreatic ductal adenocarcinoma cell line MIA PaCa-2 GR
    Article Snippet: All sequencing data were demultiplexed and converted to fastq files using bcl2fastq (Illumina Inc., San Diego, CA). .. After quality control with the FastQC tool, fastq files were processed using the QIAseq miRNA quantification feature provided by QIAGEN’s GeneGlobe Data Analysis Center (Qiagen, Germantown, MD). ..

    RNA Sequencing:

    Article Title: Statistical modeling of extracellular vesicle cargo to predict clinical trial outcomes for hypoplastic left heart syndrome
    Article Snippet: We conducted RNA library preparation and sequencing at the Emory Yerkes Nonhuman Primate Genomics core (Illumina NovaSeq 6000). .. Small RNA sequencing files were aligned using the Qiagen GeneGlobe console with QIAseq miRNA Quantification tool. ..

    other:

    Article Title: Exploration of microRNAs from blood extracellular vesicles as biomarkers of exposure to polycyclic aromatic hydrocarbons.
    Article Snippet: Exposure to polycyclic aromatic hydrocarbons (PAHs), ubiquitously environmental contaminant, leads to the development of major toxic effects on human health, such as carcinogenic and immunosuppressive alterations reported for the most studied PAH, i.e., benzo(a)pyrene (B(a)P).. In order to assess the risk associated with this exposure, it is necessary to have predictive biomarkers.. Thus, extracellular vesicles (EVs) and their microRNA (miRNA) contents, have recently been proposed as potentially interesting biomarkers in Toxicology.



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    Scatter plot of normalized <t>miRNA</t> expression in four canine lymphoma entities compared to PBMC as control group. The center diagonal line indicates unchanged miRNA expression, while the outer diagonal lines indicate the Fold regulation threshold >2.0. MicroRNAs with data points beyond the outer lines in the upper left and lower right corners are up-regulated (red dots) or down-regulated (green dots), by more than the Fold regulation threshold in the y-axis Group relative to the x-axis Group. (A) Group 3 = DLBCL vs. Control Group = PBMC; (B) Group 4 = PTCL (incl. Enteric TCL) vs. Control Group = PBMC; (C) Group 5 = TZL vs. Control Group = PBMC; (D) Group 6 = MZL vs. Control Group = PBMC.
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    Scatterplot of <t>miRNA</t> microarray analysis. CT data from samples (sEVs PBDE+LPS ) and control (sEVs DMSO+LPS ) were analyzed with the <t>GeneGlobe</t> <t>miRCURY</t> <t>LNA</t> miRNA <t>PCR</t> Data Analysis tool ( https://dataanalysis2.qiagen.com/miRCury ). MiRNAs were considered down-regulated if the fold change was <0.5 (green dot) and up-regulated if the fold change was >2 (red dots); in black are reported the unchanged miRNAs with respect to control (sEVs DMSO+LPS ). Normalization for the fold change calculation was performed using UniSp6 exogenous control and the geNorm (Predefined reference miRNA only) function.
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    Image Search Results


    Scatter plot of normalized miRNA expression in four canine lymphoma entities compared to PBMC as control group. The center diagonal line indicates unchanged miRNA expression, while the outer diagonal lines indicate the Fold regulation threshold >2.0. MicroRNAs with data points beyond the outer lines in the upper left and lower right corners are up-regulated (red dots) or down-regulated (green dots), by more than the Fold regulation threshold in the y-axis Group relative to the x-axis Group. (A) Group 3 = DLBCL vs. Control Group = PBMC; (B) Group 4 = PTCL (incl. Enteric TCL) vs. Control Group = PBMC; (C) Group 5 = TZL vs. Control Group = PBMC; (D) Group 6 = MZL vs. Control Group = PBMC.

    Journal: Frontiers in Veterinary Science

    Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

    doi: 10.3389/fvets.2024.1379146

    Figure Lengend Snippet: Scatter plot of normalized miRNA expression in four canine lymphoma entities compared to PBMC as control group. The center diagonal line indicates unchanged miRNA expression, while the outer diagonal lines indicate the Fold regulation threshold >2.0. MicroRNAs with data points beyond the outer lines in the upper left and lower right corners are up-regulated (red dots) or down-regulated (green dots), by more than the Fold regulation threshold in the y-axis Group relative to the x-axis Group. (A) Group 3 = DLBCL vs. Control Group = PBMC; (B) Group 4 = PTCL (incl. Enteric TCL) vs. Control Group = PBMC; (C) Group 5 = TZL vs. Control Group = PBMC; (D) Group 6 = MZL vs. Control Group = PBMC.

    Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

    Techniques: Expressing, Control

    Fold regulation of significantly dysregulated miRNAs in four canine lymphoma entities by using PBMC as control group.

    Journal: Frontiers in Veterinary Science

    Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

    doi: 10.3389/fvets.2024.1379146

    Figure Lengend Snippet: Fold regulation of significantly dysregulated miRNAs in four canine lymphoma entities by using PBMC as control group.

    Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

    Techniques: Control

    Normalized miRNA expression in two canine lymphoma entities. (A) Scatter plot of normalized miRNA expression comparing Group 3 (DLBCL) with Group 4 (PTCL, incl. Enteric TCL). The center diagonal line indicates unchanged miRNA expression, while the outer diagonal lines indicate the Fold regulation threshold >2.0. MicroRNAs with data points beyond the outer lines in the upper left and lower right corners are up-regulated (red dots) or down-regulated (green dots), by more than the Fold regulation threshold in the y-axis Group relative to the x-axis Group. (B) Volcano Plot illustrating significant miRNA expression changes when comparing Group 3 (DLBCL) with Group 4 (PTCL, incl. Enteric TCL). The center vertical line indicates unchanged miRNA expression, while the two outer vertical lines indicate the selected Fold regulation threshold. The horizontal line indicates the selected p -value threshold <0.05. MicroRNAs with data points in the far upper left and far upper right sections are down-regulated (green dots) or up-regulated (red dots) and meet the selected Fold regulation and p-value thresholds.

    Journal: Frontiers in Veterinary Science

    Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

    doi: 10.3389/fvets.2024.1379146

    Figure Lengend Snippet: Normalized miRNA expression in two canine lymphoma entities. (A) Scatter plot of normalized miRNA expression comparing Group 3 (DLBCL) with Group 4 (PTCL, incl. Enteric TCL). The center diagonal line indicates unchanged miRNA expression, while the outer diagonal lines indicate the Fold regulation threshold >2.0. MicroRNAs with data points beyond the outer lines in the upper left and lower right corners are up-regulated (red dots) or down-regulated (green dots), by more than the Fold regulation threshold in the y-axis Group relative to the x-axis Group. (B) Volcano Plot illustrating significant miRNA expression changes when comparing Group 3 (DLBCL) with Group 4 (PTCL, incl. Enteric TCL). The center vertical line indicates unchanged miRNA expression, while the two outer vertical lines indicate the selected Fold regulation threshold. The horizontal line indicates the selected p -value threshold <0.05. MicroRNAs with data points in the far upper left and far upper right sections are down-regulated (green dots) or up-regulated (red dots) and meet the selected Fold regulation and p-value thresholds.

    Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

    Techniques: Expressing

    Scatter plot of normalized miRNA expression in four canine lymphoma entities compared to Lymph node (LN) as control group. The center diagonal line indicates unchanged miRNA expression, while the outer diagonal lines indicate the Fold regulation threshold >2.0. MicroRNAs with data points beyond the outer lines in the upper left and lower right corners are up-regulated (red dots) or down-regulated (green dots), by more than the Fold regulation threshold in the y-axis Group relative to the x-axis Group. (A) Group 3 = DLBCL vs. Control Group = LN; (B) Group 4 = PTCL (incl. Enteric TCL) vs. Control Group = LN; (C) Group 5 = TZL vs. Control Group = LN; (D) Group 6 = MZL vs. Control Group = LN.

    Journal: Frontiers in Veterinary Science

    Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

    doi: 10.3389/fvets.2024.1379146

    Figure Lengend Snippet: Scatter plot of normalized miRNA expression in four canine lymphoma entities compared to Lymph node (LN) as control group. The center diagonal line indicates unchanged miRNA expression, while the outer diagonal lines indicate the Fold regulation threshold >2.0. MicroRNAs with data points beyond the outer lines in the upper left and lower right corners are up-regulated (red dots) or down-regulated (green dots), by more than the Fold regulation threshold in the y-axis Group relative to the x-axis Group. (A) Group 3 = DLBCL vs. Control Group = LN; (B) Group 4 = PTCL (incl. Enteric TCL) vs. Control Group = LN; (C) Group 5 = TZL vs. Control Group = LN; (D) Group 6 = MZL vs. Control Group = LN.

    Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

    Techniques: Expressing, Control

    Fold regulation of significantly dysregulated miRNAs in four canine lymphoma entities by using Lymph node as control group.

    Journal: Frontiers in Veterinary Science

    Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

    doi: 10.3389/fvets.2024.1379146

    Figure Lengend Snippet: Fold regulation of significantly dysregulated miRNAs in four canine lymphoma entities by using Lymph node as control group.

    Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

    Techniques: Control

    DLBCL and PTCL (incl. Enteric TCL) miRNA fold regulations compared between the two control groups.

    Journal: Frontiers in Veterinary Science

    Article Title: Exploratory screening for micro-RNA biomarkers in canine multicentric lymphoma

    doi: 10.3389/fvets.2024.1379146

    Figure Lengend Snippet: DLBCL and PTCL (incl. Enteric TCL) miRNA fold regulations compared between the two control groups.

    Article Snippet: The following miRNAs were suggested by the miRNA PCR Data Analysis Software (Qiagen) for further validation as potential biomarkers for canine DLBCL: miR-15b, miR-18a, miR-26b, miR-34a, miR-150, miR-181c, miR-223, miR-363, miR-423a, and miR-450b.

    Techniques: Control

    Scatterplot of miRNA microarray analysis. CT data from samples (sEVs PBDE+LPS ) and control (sEVs DMSO+LPS ) were analyzed with the GeneGlobe miRCURY LNA miRNA PCR Data Analysis tool ( https://dataanalysis2.qiagen.com/miRCury ). MiRNAs were considered down-regulated if the fold change was <0.5 (green dot) and up-regulated if the fold change was >2 (red dots); in black are reported the unchanged miRNAs with respect to control (sEVs DMSO+LPS ). Normalization for the fold change calculation was performed using UniSp6 exogenous control and the geNorm (Predefined reference miRNA only) function.

    Journal: Frontiers in Immunology

    Article Title: Impact of the flame retardant 2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) in THP-1 macrophage-like cell function via small extracellular vesicles

    doi: 10.3389/fimmu.2022.1069207

    Figure Lengend Snippet: Scatterplot of miRNA microarray analysis. CT data from samples (sEVs PBDE+LPS ) and control (sEVs DMSO+LPS ) were analyzed with the GeneGlobe miRCURY LNA miRNA PCR Data Analysis tool ( https://dataanalysis2.qiagen.com/miRCury ). MiRNAs were considered down-regulated if the fold change was <0.5 (green dot) and up-regulated if the fold change was >2 (red dots); in black are reported the unchanged miRNAs with respect to control (sEVs DMSO+LPS ). Normalization for the fold change calculation was performed using UniSp6 exogenous control and the geNorm (Predefined reference miRNA only) function.

    Article Snippet: The CT data obtained from control and treated cells were analyzed using the Qiagen GeneGlobe miRCURY LNA miRNA PCR Data Analysis software tool, and the expression values were reported as fold change.

    Techniques: Microarray, Control

    Scatterplot of miRNA expression in M(0) THP-1 macrophage-like cells treated with sEVs PBDE+LPS . CT values obtained from three independent experiments (M(0) THP-1 macrophage-like treated with sEVs PBDE+LPS vs controls) were analyzed using the GeneGlobe miRCURY LNA miRNA PCR Data Analysis tool. Blue dots show down-regulated miRNAs (fold change <0.5), red is for up-regulated miRNA (fold change >2), and black indicates unchanged miRNAs with respect to control cells.

    Journal: Frontiers in Immunology

    Article Title: Impact of the flame retardant 2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) in THP-1 macrophage-like cell function via small extracellular vesicles

    doi: 10.3389/fimmu.2022.1069207

    Figure Lengend Snippet: Scatterplot of miRNA expression in M(0) THP-1 macrophage-like cells treated with sEVs PBDE+LPS . CT values obtained from three independent experiments (M(0) THP-1 macrophage-like treated with sEVs PBDE+LPS vs controls) were analyzed using the GeneGlobe miRCURY LNA miRNA PCR Data Analysis tool. Blue dots show down-regulated miRNAs (fold change <0.5), red is for up-regulated miRNA (fold change >2), and black indicates unchanged miRNAs with respect to control cells.

    Article Snippet: The CT data obtained from control and treated cells were analyzed using the Qiagen GeneGlobe miRCURY LNA miRNA PCR Data Analysis software tool, and the expression values were reported as fold change.

    Techniques: Expressing, Control