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tsaye supplemented with 5% nacl (wt/vol)  (Applichem inc)

 
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    Applichem inc tsaye supplemented with 5% nacl (wt/vol)
    Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. <t>Total</t> <t>culturable</t> (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on <t>TSAYE</t> supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.
    Tsaye Supplemented With 5% Nacl (Wt/Vol), supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/milk+blocking+buffer/blocking+buffer+pbs+with+5++skimmed+milk+powder/pmc08672913-223-12-18
    Average 90 stars, based on 1 article reviews
    tsaye supplemented with 5% nacl (wt/vol) - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Listeria monocytogenes Sublethal Injury and Viable-but-Nonculturable State Induced by Acidic Conditions and Disinfectants"

    Article Title: Listeria monocytogenes Sublethal Injury and Viable-but-Nonculturable State Induced by Acidic Conditions and Disinfectants

    Journal: Microbiology Spectrum

    doi: 10.1128/Spectrum.01377-21

    Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.
    Figure Legend Snippet: Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, 3, 4, or 5 h under the following conditions: (A) AA, pH 3.0, at 20°C, (B) AA, pH 2.7, at 20°C, (C) AA, pH 2.5, at 20°C, (D) AA, pH 3.0, at 4°C, (E) AA, pH 2.7, at 4°C, (F) AA, pH 2.5, at 4°C, (G) HCl, pH 3.0, at 20°C, (H) HCl, pH 2.7, at 20°C, (I) HCl, pH 2.5, at 20°C, (J) HCl, pH 3.0, at 4°C, (K) HCl, pH 2.7, at 4°C, and (L) HCl, pH 2.5, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± standard deviations (SD) from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). **, P < 0.05; ***, P < 0.01; n.s., not significant.

    Techniques Used: Incubation

    Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 30, 60, 300, 900, or 1,800 s under the following conditions: (A) PAA, 0.5 ppm, at 20°C, (B) PAA, 5 ppm, at 20°C, (C) PAA, 10 ppm, at 20°C, (D) PAA, 0.5 ppm, at 4°C, (E) PAA, 5 ppm, at 4°C, (F) PAA, 10 ppm, at 4°C, (G) SH, 0.5 ppm, at 20°C, (H) SH, 5 ppm, at 20°C, (I) SH, 10 ppm, at 20°C, (J) SH, 0.5 ppm, at 4°C, (K) SH, 5 ppm, at 4°C, and (L) SH, 10 ppm, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). *, P < 0.1; **, P < 0.05; ***, P < 0.01; n.s., not significant.
    Figure Legend Snippet: Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 30, 60, 300, 900, or 1,800 s under the following conditions: (A) PAA, 0.5 ppm, at 20°C, (B) PAA, 5 ppm, at 20°C, (C) PAA, 10 ppm, at 20°C, (D) PAA, 0.5 ppm, at 4°C, (E) PAA, 5 ppm, at 4°C, (F) PAA, 10 ppm, at 4°C, (G) SH, 0.5 ppm, at 20°C, (H) SH, 5 ppm, at 20°C, (I) SH, 10 ppm, at 20°C, (J) SH, 0.5 ppm, at 4°C, (K) SH, 5 ppm, at 4°C, and (L) SH, 10 ppm, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). *, P < 0.1; **, P < 0.05; ***, P < 0.01; n.s., not significant.

    Techniques Used: Incubation

    Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 30, 60, 300, 900, or 1,800 s under the following conditions: (A) PAA, 20 ppm, at 20°C, (B) PAA, 30 ppm, at 20°C, (C) PAA, 40 ppm, at 20°C, (D) PAA, 20 ppm, at 4°C, (E) PAA, 30 ppm, at 4°C, and (F) PAA, 40 ppm, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). *, P < 0.1; **, P < 0.05; n.s., not significant.
    Figure Legend Snippet: Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 30, 60, 300, 900, or 1,800 s under the following conditions: (A) PAA, 20 ppm, at 20°C, (B) PAA, 30 ppm, at 20°C, (C) PAA, 40 ppm, at 20°C, (D) PAA, 20 ppm, at 4°C, (E) PAA, 30 ppm, at 4°C, and (F) PAA, 40 ppm, at 4°C. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 days and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from 3 independent biological replicates. Statistically significant differences ( P < 0.05) are indicated with different uppercase (TSAYE) and lowercase (TSAYE+5%NaCl) letters, respectively. The dotted line represents the limit of detection (LOD). *, P < 0.1; **, P < 0.05; n.s., not significant.

    Techniques Used: Incubation

    Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, or 3 h at 20°C in Ringer’s solution with 20 ppm (A), 30 ppm (C), or 40 ppm (E) PAA. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from one experiment with six independent samples ( n = 6). Statistically significant differences ( P < 0.05) are indicated with different uppercase and lowercase letters for TSAYE and TSAYE+5%NaCl, respectively. The dotted line represents the limit of detection (LOD). ***, P < 0.01; n.s., not significant. Each time point relates to several images acquired at different fields of view (FOV) of the same sample. Enumeration results (%) for cells exhibiting fluorescence (CFDA + /PI − , green; CFDA − /PI + , red; CFDA + /PI + , orange; or CFDA − /PI − , black), considering several images of different fields of view (FOV) at each time point after treatment with PAA at 20 ppm (0 h, 5 FOV; 1 h, 4 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (B), 30 ppm (0 h, 4 FOV; 1 h, 5 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (D), and 40 ppm (0 h, 4 FOV; 1 h, 5 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (F).
    Figure Legend Snippet: Populations (log CFU/ml) of L. monocytogenes , strain Scott A, after incubation for 0, 1, 2, or 3 h at 20°C in Ringer’s solution with 20 ppm (A), 30 ppm (C), or 40 ppm (E) PAA. Total culturable (dark gray bars) and noninjured (light gray bars) population levels were measured after incubation for 2 and 5 days at 37°C on TSAYE supplemented with 0% and 5% NaCl, respectively. Bars represent mean values ± SD from one experiment with six independent samples ( n = 6). Statistically significant differences ( P < 0.05) are indicated with different uppercase and lowercase letters for TSAYE and TSAYE+5%NaCl, respectively. The dotted line represents the limit of detection (LOD). ***, P < 0.01; n.s., not significant. Each time point relates to several images acquired at different fields of view (FOV) of the same sample. Enumeration results (%) for cells exhibiting fluorescence (CFDA + /PI − , green; CFDA − /PI + , red; CFDA + /PI + , orange; or CFDA − /PI − , black), considering several images of different fields of view (FOV) at each time point after treatment with PAA at 20 ppm (0 h, 5 FOV; 1 h, 4 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (B), 30 ppm (0 h, 4 FOV; 1 h, 5 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (D), and 40 ppm (0 h, 4 FOV; 1 h, 5 FOV; 2 h, 5 FOV; 3 h, 5 FOV) (F).

    Techniques Used: Incubation, Fluorescence

    Related Articles

    other:

    Article Title: Listeria monocytogenes Sublethal Injury and Viable-but-Nonculturable State Induced by Acidic Conditions and Disinfectants
    Article Snippet: To differentiate the injured subpopulation from the total culturable population, growth in TSAYE supplemented with 5% NaCl (wt/vol) (AppliChem) was compared to growth in TSAYE with 0.5% NaCl.

    Article Title: Assessing the survival and sublethal injury kinetics of Listeria monocytogenes under different food processing-related stresses.
    Article Snippet: The foodborne pathogen L. monocytogenes can be present in food processing environments where it is exposed to various stressors.. These antimicrobial factors, which aim to eliminate the pathogen, can induce sub-lethal injury to the bacterial cells.. In the present study, we investigated the efficacy of different treatments (stresses) relevant to food processing and preservation as well as sanitation methods, in generating sub-lethal injury at 4 C and 20 C to two L. monocytogenes strains, ScottA and EGDe.

    Article Title: Deciphering the induction of Listeria monocytogenes into sublethal injury using fluorescence microscopy and RT-qPCR.
    Article Snippet: The adaptive response of bacterial cells to changing environmental conditions depends on the behavior of single cells within the population.. Exposure of Listeria monocytogenes to sublethal acidic conditions in foods or in the gastrointestinal track of the host may induce injuries relevant to difficult physiological states within the dormancy continuum.. In this study, exposure to acidic conditions (acetic-AA and hydrochloric acid-HCl adjusted to pH 3.0, 2.7, 2.5 at 20 C for 5 h) was used to evaluate injury of L. monocytogenes, Scott A strain.

    Bacteria:

    Article Title: Detection of Actinobacillus pleuropneumoniae in pigs by real-time quantitative PCR for the apxIVA gene.
    Article Snippet: 1090-0233/$ see front matter 2012 Elsevier Ltd. A http://dx.doi.org/10.1016/j.tvjl.2012.02.004 ⇑ Corresponding author.. Tel.. : +31 3

    Electrotransfer:

    Article Title: Transforming Growth Factor Beta (TGF-β1) Induces Pro-Reparative Phenotypic Changes in Epicardial Cells in Mice.
    Article Snippet: We evaluated the content of active form of TGF-β1 in the intact and post-infarction heart and the effect of this factor on the properties of epicardial cells.. During the acute stage after myocardial infarction, the production of TGF-β1 in the heart increased, which closely correlated with activation of epicardial cells (appearance of a pool of Wt1+ epicardial cells entering the epithelial-mesenchymal transition).. The role of TGF-β1 as the factor of epicardial activation was confirmed by the results of in vitro experiments: addition of recombinant TGF-β1 to cultured epicardial cells led to enhanced expression of genes of epithelial-mesenchymal transition and phenotypic transformation of these cells leading to the appearance of cells with markers of smooth muscle cells and fibroblasts.

    Membrane:

    Article Title: Transforming Growth Factor Beta (TGF-β1) Induces Pro-Reparative Phenotypic Changes in Epicardial Cells in Mice.
    Article Snippet: We evaluated the content of active form of TGF-β1 in the intact and post-infarction heart and the effect of this factor on the properties of epicardial cells.. During the acute stage after myocardial infarction, the production of TGF-β1 in the heart increased, which closely correlated with activation of epicardial cells (appearance of a pool of Wt1+ epicardial cells entering the epithelial-mesenchymal transition).. The role of TGF-β1 as the factor of epicardial activation was confirmed by the results of in vitro experiments: addition of recombinant TGF-β1 to cultured epicardial cells led to enhanced expression of genes of epithelial-mesenchymal transition and phenotypic transformation of these cells leading to the appearance of cells with markers of smooth muscle cells and fibroblasts.

    Incubation:

    Article Title: Transforming Growth Factor Beta (TGF-β1) Induces Pro-Reparative Phenotypic Changes in Epicardial Cells in Mice.
    Article Snippet: We evaluated the content of active form of TGF-β1 in the intact and post-infarction heart and the effect of this factor on the properties of epicardial cells.. During the acute stage after myocardial infarction, the production of TGF-β1 in the heart increased, which closely correlated with activation of epicardial cells (appearance of a pool of Wt1+ epicardial cells entering the epithelial-mesenchymal transition).. The role of TGF-β1 as the factor of epicardial activation was confirmed by the results of in vitro experiments: addition of recombinant TGF-β1 to cultured epicardial cells led to enhanced expression of genes of epithelial-mesenchymal transition and phenotypic transformation of these cells leading to the appearance of cells with markers of smooth muscle cells and fibroblasts.

    Blocking Assay:

    Article Title: Transforming Growth Factor Beta (TGF-β1) Induces Pro-Reparative Phenotypic Changes in Epicardial Cells in Mice.
    Article Snippet: We evaluated the content of active form of TGF-β1 in the intact and post-infarction heart and the effect of this factor on the properties of epicardial cells.. During the acute stage after myocardial infarction, the production of TGF-β1 in the heart increased, which closely correlated with activation of epicardial cells (appearance of a pool of Wt1+ epicardial cells entering the epithelial-mesenchymal transition).. The role of TGF-β1 as the factor of epicardial activation was confirmed by the results of in vitro experiments: addition of recombinant TGF-β1 to cultured epicardial cells led to enhanced expression of genes of epithelial-mesenchymal transition and phenotypic transformation of these cells leading to the appearance of cells with markers of smooth muscle cells and fibroblasts.



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