midi prep kit (Qiagen)

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Qiagen midi prep kit
Midi Prep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5887 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/midi+prep+kit/QIAGEN+Plasmid+Midi+Kit/pmc12979480-44-8-11
Average 99 stars, based on 5887 article reviews

midi prep kit - by Bioz Stars, 2026-11

99/100 stars

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Purification:

Article Title: Septins regulate cytokinesis and multicellular development in the closest living relatives of animals
Article Snippet: The amplified H2B coding sequence was assembled with the digested pMC111 backbone using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Cat. No. E2621L). .. Verified transformants were re-transformed into dam−/dcm− competent E. coli (New England Biolabs, Cat. No. C2925H) and purified for transfection into S. rosetta by midiprep (Qiagen, Cat. No. 12143) followed by ethanol precipitation of the DNA. .. The resulting plasmid is available at Addgene (Addgene ID NK813; Cat. No. 257627).

Article Title: The Role of Glucose Metabolism in Xenopus Primitive Myeloid Cell Development and Function.
Article Snippet: .. Plasmid DNA was purified from bacterial cultures using the Plasmid Midi Kit (Qiagen, Hilden, Germany), linearized with Sal I as listed in Table 1, and purified by phenol–chloroform extraction and isopropanol precipitation. ..

Transfection:

Article Title: PAX6ΔPD modulates the balance between proliferation and differentiation programs in corneal epithelial cells through protein-protein interactions.
Article Snippet: .. For transfection experiments, plasmids DNA were isolated with the Plasmid Midi kit (Qiagen Inc., Valencia, CA). ..

Ethanol Precipitation:

Affinity Purification:

Article Title: Circadian control of dopaminergic signaling to the mushroom body regulates sleep through rhythmic Pka-C1 transcription in Drosophila.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat affinity purified anti-GFP Nacalai Tesque Cat# 04404-84; RRID: AB_10013361 Rabbit Anti-TH Millipore Cat# AB152; RRID: AB_390204 Mouse Anti-RFP Antibodies.com Cat# A85284; RRID: AB_2752916 Rabbit Anti-PER Gift from Rosbash lab59 N/A Rabbit Anti-Onecut Cusabio Cat# CSB-PA889056XA01DLU Alexa 633 goat Anti-mouse IgG Thermo Fisher Scientific Cat#A-21052; RRID: AB_2535719 Alexa 488 goat Anti-rat IgG Thermo Fisher Scientific Cat# A-21208; RRID: AB_2535794 Alexa 568 goat Anti-rabbit IgG Thermo Fisher Scientific Cat# A-78955; RRID: AB_2925778 Alexa 488 goat Anti-rabbit IgG Thermo Fisher Scientific Cat# A-11008; RRID: AB_143165 Bacterial and virus strains Escherichia coli DH5α New England Biolabs Cat# C2987H Chemicals, peptides, and recombinant proteins D-luciferin potassium salt Biosynth Cat# L-8220 ATP Sigma-Aldrich Cat# A2383-5G KOD polymerase Sigma-Aldrich Cat#71085 Q5 Hot Start High-Fidelity 2x master mix New England Biolabs Cat# M0494S Dynabeads Protein G Thermo Fisher Scientific Cat# 10003D RNase A Roche Cat# 10154105103 Igepal CA-630 Sigma-Aldrich Cat# I8896 Na3VO4 Sigma-Aldrich Cat# 567540 PMSF Sigma-Aldrich CAS: 329-98-6 BSA Sigma-Aldrich Cat# 128910 Glycogen Thermo Fisher Scientific Cat# R0561 Salmon sperm DNA, 25mg Rockland Cat# MB-103-0025 T4 DNA Ligase New England Biolabs Cat# M0202S Protease inhibitor cocktail Roche Cat# 11 873 580 001 Critical commercial assays Gibson Assembly Cloning Kit, 10 rxns New England Biolabs Cat# E5510S Monarch Gel Dissolving Buffer New England Biolabs Cat# T1021L Monarch PCR and DNA Cleanup Kit (5ug) New England Biolabs Cat# T1030L Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 QIAGEN Plasmid Midi Kit QIAGEN Cat# 12143 Custom TaqMan Gene Expression Assay (OnecutBS) Thermo Fisher Scientific Cat# 4332077; Assay ID: ANT2VMX Experimental models: Organisms/strains w[1118]; P{y[+t7.7] w[+mC]=R13F02-p65.AD}attP40/CyO; P{y[+t7.7] w[+mC]=R45H04-GAL4.DBD}attP2 BDSC RRID:BDSC_68292 w[1118]; P{y[+t7.7] w[+mC]=R26E07-p65.AD}attP40/CyO; P{y[+t7.7] w[+mC]=R39A11-GAL4.DBD}attP2 BDSC RRID:BDSC_68323 w[1118]; P{y[+t7.7] w[+mC]=R13F02-p65.AD}attP40/CyO; P{y[+t7.7] w[+mC]=R89B01-GAL4.DBD}attP2 BDSC RRID:BDSC_68265 w[1118]; P{y[+t7.7] w[+mC]=R58E02-p65.AD}attP40; P{y[+t7.7] w[+mC]=R31F06-GAL4.DBD}attP2 BDSC RRID:BDSC_68270 w[1118]; P{y[+t7.7] w[+mC]=R30G08-p65.AD}attP40; P{y[+t7.7] w[+mC]=R48B03-GAL4.DBD}attP2 BDSC RRID:BDSC_68251 (Continued on next page) ll OPEN ACCESS Current Biology 36, 1–18.e1–e7, October 19, 2026 e1 Article ..

Virus:

Recombinant:

Article Title: Small molecules rescue pathogenic POLGA aggregation and restore its functions in a mouse model of PEO.
Article Snippet: com/en/product/cellanalysis/real-time-cellmetabolic-analysis/xfsoftware/seahorsewave-desktop-software740897 FlowJo v10 https://www.flowjo. .. com/ Other HOOK-Biotin-PEG2Amine Labelling Kit G-Biosciences Cat# BS-16 Mitochondrial DNA isolation kit BioVision Inc. Cat# K280-50 QuikChange II XL SiteDirected Mutagenesis Kit Agilent Cat# 200522 QIAamp DNA Mini Kit Qiagen Cat# 51304 QIAamp DNA Midi Kit Qiagen Cat# 12143 Reagents, antibodies, primers and recombinants All antibodies, reagents (including chemicals, recombinant proteins, cell lines, peptides and oligonucleotides), animals, deposited data and other materials used are described in structured methods. ..

Protease Inhibitor:

Cloning:

Polymerase Chain Reaction:

BIA-KA:

Plasmid Preparation:

Article Title: Acidic transcription factors position the genome at nuclear speckles through transcription dependent and independent mechanisms
Article Snippet: Dneasy Blood & Tissue kit , QIAGEN , 69504. .. QIAGEN Plasmid Midi Kit , QIAGEN , 12143. .. QIAprep Spin Miniprep Kit , QIAGEN , 27106.

Article Title: Metabolic resistance to emamectin benzoate mediated by CYP4C175 and its dual regulatory functions in development and reproduction of Megalurothrips usitatus.
Article Snippet: The CYP4C175 gene was cloned and ligated into the pJFRC28-10XUAS-IVS-GFP-p10 (Addgene 36 431) plasmid vector. .. Plasmid DNAwas extracted utilizing QIAGEN PlasmidMidi Kits (12143), diluted to 500 ng μL−1 with ddH2O, and 30 μL aliquots were prepared for microinjection procedures. ..

Article Title: Pharmacological inhibition of MCL-1 disrupts mitochondrial cristae and depletes the human neural progenitor cell pool.
Article Snippet: The following day, bacterial colonies were picked and grown in 10 ml of LB broth overnight at 37°C, followed by a QIAprep Spin Miniprep (Qiagen, 27104) to isolate plasmid DNA. .. Sequencing of the clones was performed by GeneWiz, and the corrected lentiviral clones with the ACSL1–HaloTag insert were selected and expanded using the QIAGEN Plasmid Midi Kit (Qiagen, 12143). .. The pLV-EF1a-ACSL-1-HaloTagPGK-Puro plasmid is available through Addgene (Addgene plasmid 257893).

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midi prep kit (Qiagen)

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Related Articles

Purification:

Transfection:

Ethanol Precipitation:

Affinity Purification:

Virus:

Recombinant:

Protease Inhibitor:

Cloning:

Polymerase Chain Reaction:

BIA-KA:

Plasmid Preparation:

Gene Expression:

Isolation:

Microinjection:

Sequencing:

Clone Assay:

Extraction:

DNA Extraction:

Mutagenesis:

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