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zymopure ii plasmid midi prep kit  (Zymo Research)


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    Zymo Research zymopure ii plasmid midi prep kit
    Zymopure Ii Plasmid Midi Prep Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/midi+prep+kit/ZymoPURE+II+Plasmid+Midiprep+Kit/pmc12908608-76-23-29
    Average 99 stars, based on 612 article reviews
    zymopure ii plasmid midi prep kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Purification:

    Article Title: PALINCODE: Recording cell lineage with ternary palindromic CRISPR bits
    Article Snippet: .. We purified the plasmid by Zymo midiprep #D4200 for high-diversity UMI plasmid production. .. We purified the plasmid by Zymo midiprep #D4200 for high-diversity UMI plasmid production.

    Article Title: Systematic mapping of enhancers, silencers, and topological elements with prime editing deletion screens
    Article Snippet: We purified the Gibson assembly with 0.7X Ampure XP SPRI and eluted in 11 uL, then electroporated 10 uL into Endura competent cells (Lucigen #602422) using the 1 mm cuvette with the Bio-Rad micro pulser electroporation system (1.8 kV, 600Ω, 10 μF). .. We performed a serial dilution of 10 uL of the electroporated bacteria and spread it on agar plates containing carbenicillin, and expanded the rest in liquid culture containing carbenicillin for 20 hours at 30°C, then the library plasmid was extracted and purified with the ZymoPURE II Plasmid Midiprep Kit (Zymo Research #D4201). ..

    Article Title: Protocol for live-cell imaging and analysis of NF-κB localization and target gene transcription in Drosophila S2∗ cells.
    Article Snippet: LB media Thermo Fisher Scientific 12780052 Schneider’s Drosophila Medium Thermo Fisher Scientific 21720024 FBS, heat inactivated Thermo Fisher Scientific MT35010CV L-glutamine (200mM) Thermo Fisher Scientific 25030081 Penicillin-Streptomycin (10000 U/mL) Thermo Fisher Scientific 15140122 Fungizone Thermo Fisher Scientific 15290018 Trypan Blue Thermo Fisher Scientific 15250061 Puromycin InvivoGen ant-pr-1 Blasticidin powder Fisher Scientific BP264725 Electroporation Buffer MaxCyte EPB-5 TURBO DNase (2 U/μL) Thermo Fisher Scientific AM2238 Alt-R S.p. .. HiFi Cas9 Nuclease V3 (62 μM stock) IDT 1081060 Alt-R CRISPR-Cas9 tracrRNA (5 nmol) IDT 1072533 20-hydroxyecdysone Sigma-Aldrich H5142 Concanavalin A Sigma-Aldrich C5275 Purified DAP-type PGN Sigma-Aldrich 69554 Hoechst 33258 Thermo Fisher Scientific H1398 JFx650 (HaloTag Ligand) Promega/Tocris HT1070 SpyRHO555 (RNA Aptamer Dye) Cytoskeleton CY-SC018 Anhydrous DMSO Thermo Fisher Scientific D12345 Critical commercial assays ZymoPURE II Plasmid Midiprep Kit Zymo Research D4200 Experimental models: Cell lines S2* Laboratory of Steve Wasserman.14 Alternative source: zeba@bu.edu Species: D. melanogaster; Cell line sex: male. ..

    Article Title: Discovery of MYC/NSD3S Protein–Protein Interaction Negative Modulators with a Live Cell Nanoluciferase Protein-Fragment Complementation uHTS Assay
    Article Snippet: .. Plasmid purification was performed using the ZymoPURE Plasmid Maxiprep Kit (Zymo Research, cat. no. D4200). .. The DNA concentration was quantified using a spectrophotometer (NanoDrop Lite, Thermo Scientific).

    Article Title: MicroRNA Regulation of the Estrogen Receptor Alpha 3′UTR in Canine Mammary Tumor (CMT28) Cells
    Article Snippet: .. Plasmid DNA was purified using the ZymoPURE Midiprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s instructions. ..

    Plasmid Preparation:

    Article Title: PALINCODE: Recording cell lineage with ternary palindromic CRISPR bits
    Article Snippet: .. We purified the plasmid by Zymo midiprep #D4200 for high-diversity UMI plasmid production. .. We purified the plasmid by Zymo midiprep #D4200 for high-diversity UMI plasmid production.

    Article Title: Spectral tuning and signaling of diverse and far red-sensitive rhodopsins in mantis shrimp eyes.
    Article Snippet: .. All final plasmids were sequence verified by Sanger sequencing, and endotoxin- free plasmid stocks were prepared using the Zymo Midi- prep procedure (Zymo Research) before transfection. .. HEK293T cells (Life Technologies) were used for opsin purification; Gq, Gi/o, and Gs live bioluminescent second messenger assays and HEK293 lacking GNAS/GNAL/GNAQ/GNA11/GNA12/GNA13/ GNAZ (HEK293ΔG7) (69) were used for testing more specifically the Gα signaling selectivity in GsX and GqX assays.

    Article Title: Systematic mapping of enhancers, silencers, and topological elements with prime editing deletion screens
    Article Snippet: We purified the Gibson assembly with 0.7X Ampure XP SPRI and eluted in 11 uL, then electroporated 10 uL into Endura competent cells (Lucigen #602422) using the 1 mm cuvette with the Bio-Rad micro pulser electroporation system (1.8 kV, 600Ω, 10 μF). .. We performed a serial dilution of 10 uL of the electroporated bacteria and spread it on agar plates containing carbenicillin, and expanded the rest in liquid culture containing carbenicillin for 20 hours at 30°C, then the library plasmid was extracted and purified with the ZymoPURE II Plasmid Midiprep Kit (Zymo Research #D4201). ..

    Article Title: Protocol for live-cell imaging and analysis of NF-κB localization and target gene transcription in Drosophila S2∗ cells.
    Article Snippet: LB media Thermo Fisher Scientific 12780052 Schneider’s Drosophila Medium Thermo Fisher Scientific 21720024 FBS, heat inactivated Thermo Fisher Scientific MT35010CV L-glutamine (200mM) Thermo Fisher Scientific 25030081 Penicillin-Streptomycin (10000 U/mL) Thermo Fisher Scientific 15140122 Fungizone Thermo Fisher Scientific 15290018 Trypan Blue Thermo Fisher Scientific 15250061 Puromycin InvivoGen ant-pr-1 Blasticidin powder Fisher Scientific BP264725 Electroporation Buffer MaxCyte EPB-5 TURBO DNase (2 U/μL) Thermo Fisher Scientific AM2238 Alt-R S.p. .. HiFi Cas9 Nuclease V3 (62 μM stock) IDT 1081060 Alt-R CRISPR-Cas9 tracrRNA (5 nmol) IDT 1072533 20-hydroxyecdysone Sigma-Aldrich H5142 Concanavalin A Sigma-Aldrich C5275 Purified DAP-type PGN Sigma-Aldrich 69554 Hoechst 33258 Thermo Fisher Scientific H1398 JFx650 (HaloTag Ligand) Promega/Tocris HT1070 SpyRHO555 (RNA Aptamer Dye) Cytoskeleton CY-SC018 Anhydrous DMSO Thermo Fisher Scientific D12345 Critical commercial assays ZymoPURE II Plasmid Midiprep Kit Zymo Research D4200 Experimental models: Cell lines S2* Laboratory of Steve Wasserman.14 Alternative source: zeba@bu.edu Species: D. melanogaster; Cell line sex: male. ..

    Article Title: Inorganic arsenic promotes bacterial tolerance to antibiotics in community-derived Escherichia coli isolates
    Article Snippet: .. Plasmid extraction was carried out using the ZymoPure PlasmidMidiprep Kit (Zymo Research) following themanufacturer’s instructions. .. The extracted plasmid was treatedwith the RecBCD enzyme (New England Biolabs) to remove any genomic DNA from the sample.

    Article Title: Discovery of MYC/NSD3S Protein–Protein Interaction Negative Modulators with a Live Cell Nanoluciferase Protein-Fragment Complementation uHTS Assay
    Article Snippet: .. Plasmid purification was performed using the ZymoPURE Plasmid Maxiprep Kit (Zymo Research, cat. no. D4200). .. The DNA concentration was quantified using a spectrophotometer (NanoDrop Lite, Thermo Scientific).

    Article Title: MicroRNA Regulation of the Estrogen Receptor Alpha 3′UTR in Canine Mammary Tumor (CMT28) Cells
    Article Snippet: .. Plasmid DNA was purified using the ZymoPURE Midiprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s instructions. ..

    Sequencing:

    Article Title: Spectral tuning and signaling of diverse and far red-sensitive rhodopsins in mantis shrimp eyes.
    Article Snippet: .. All final plasmids were sequence verified by Sanger sequencing, and endotoxin- free plasmid stocks were prepared using the Zymo Midi- prep procedure (Zymo Research) before transfection. .. HEK293T cells (Life Technologies) were used for opsin purification; Gq, Gi/o, and Gs live bioluminescent second messenger assays and HEK293 lacking GNAS/GNAL/GNAQ/GNA11/GNA12/GNA13/ GNAZ (HEK293ΔG7) (69) were used for testing more specifically the Gα signaling selectivity in GsX and GqX assays.

    DNA Sequencing:

    Article Title: Spectral tuning and signaling of diverse and far red-sensitive rhodopsins in mantis shrimp eyes.
    Article Snippet: .. All final plasmids were sequence verified by Sanger sequencing, and endotoxin- free plasmid stocks were prepared using the Zymo Midi- prep procedure (Zymo Research) before transfection. .. HEK293T cells (Life Technologies) were used for opsin purification; Gq, Gi/o, and Gs live bioluminescent second messenger assays and HEK293 lacking GNAS/GNAL/GNAQ/GNA11/GNA12/GNA13/ GNAZ (HEK293ΔG7) (69) were used for testing more specifically the Gα signaling selectivity in GsX and GqX assays.

    Transfection:

    Article Title: Spectral tuning and signaling of diverse and far red-sensitive rhodopsins in mantis shrimp eyes.
    Article Snippet: .. All final plasmids were sequence verified by Sanger sequencing, and endotoxin- free plasmid stocks were prepared using the Zymo Midi- prep procedure (Zymo Research) before transfection. .. HEK293T cells (Life Technologies) were used for opsin purification; Gq, Gi/o, and Gs live bioluminescent second messenger assays and HEK293 lacking GNAS/GNAL/GNAQ/GNA11/GNA12/GNA13/ GNAZ (HEK293ΔG7) (69) were used for testing more specifically the Gα signaling selectivity in GsX and GqX assays.

    Serial Dilution:

    Article Title: Systematic mapping of enhancers, silencers, and topological elements with prime editing deletion screens
    Article Snippet: We purified the Gibson assembly with 0.7X Ampure XP SPRI and eluted in 11 uL, then electroporated 10 uL into Endura competent cells (Lucigen #602422) using the 1 mm cuvette with the Bio-Rad micro pulser electroporation system (1.8 kV, 600Ω, 10 μF). .. We performed a serial dilution of 10 uL of the electroporated bacteria and spread it on agar plates containing carbenicillin, and expanded the rest in liquid culture containing carbenicillin for 20 hours at 30°C, then the library plasmid was extracted and purified with the ZymoPURE II Plasmid Midiprep Kit (Zymo Research #D4201). ..

    Bacteria:

    Article Title: Systematic mapping of enhancers, silencers, and topological elements with prime editing deletion screens
    Article Snippet: We purified the Gibson assembly with 0.7X Ampure XP SPRI and eluted in 11 uL, then electroporated 10 uL into Endura competent cells (Lucigen #602422) using the 1 mm cuvette with the Bio-Rad micro pulser electroporation system (1.8 kV, 600Ω, 10 μF). .. We performed a serial dilution of 10 uL of the electroporated bacteria and spread it on agar plates containing carbenicillin, and expanded the rest in liquid culture containing carbenicillin for 20 hours at 30°C, then the library plasmid was extracted and purified with the ZymoPURE II Plasmid Midiprep Kit (Zymo Research #D4201). ..

    CRISPR:

    Article Title: Protocol for live-cell imaging and analysis of NF-κB localization and target gene transcription in Drosophila S2∗ cells.
    Article Snippet: LB media Thermo Fisher Scientific 12780052 Schneider’s Drosophila Medium Thermo Fisher Scientific 21720024 FBS, heat inactivated Thermo Fisher Scientific MT35010CV L-glutamine (200mM) Thermo Fisher Scientific 25030081 Penicillin-Streptomycin (10000 U/mL) Thermo Fisher Scientific 15140122 Fungizone Thermo Fisher Scientific 15290018 Trypan Blue Thermo Fisher Scientific 15250061 Puromycin InvivoGen ant-pr-1 Blasticidin powder Fisher Scientific BP264725 Electroporation Buffer MaxCyte EPB-5 TURBO DNase (2 U/μL) Thermo Fisher Scientific AM2238 Alt-R S.p. .. HiFi Cas9 Nuclease V3 (62 μM stock) IDT 1081060 Alt-R CRISPR-Cas9 tracrRNA (5 nmol) IDT 1072533 20-hydroxyecdysone Sigma-Aldrich H5142 Concanavalin A Sigma-Aldrich C5275 Purified DAP-type PGN Sigma-Aldrich 69554 Hoechst 33258 Thermo Fisher Scientific H1398 JFx650 (HaloTag Ligand) Promega/Tocris HT1070 SpyRHO555 (RNA Aptamer Dye) Cytoskeleton CY-SC018 Anhydrous DMSO Thermo Fisher Scientific D12345 Critical commercial assays ZymoPURE II Plasmid Midiprep Kit Zymo Research D4200 Experimental models: Cell lines S2* Laboratory of Steve Wasserman.14 Alternative source: zeba@bu.edu Species: D. melanogaster; Cell line sex: male. ..

    Extraction:

    Article Title: Inorganic arsenic promotes bacterial tolerance to antibiotics in community-derived Escherichia coli isolates
    Article Snippet: .. Plasmid extraction was carried out using the ZymoPure PlasmidMidiprep Kit (Zymo Research) following themanufacturer’s instructions. .. The extracted plasmid was treatedwith the RecBCD enzyme (New England Biolabs) to remove any genomic DNA from the sample.

    other:

    Article Title: Oligo(uridine) tailing and exonucleolytic decay drive 40S ribosome degradation in response to stress.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-DIS3L2 Abcam Cat# ab181743; RRID N/A Rabbit polyclonal anti-TUT7 Cell Signaling Technology Cat#46970; RRID N/A Rabbit monoclonal anti-TUT4 Cell Signaling Technology Cat#15627; RRID N/A Rabbit polyclonal anti-uS3 Abcam Cat# ab140688; RRID: AB_2942098 Rabbit polyclonal anti-eS26 Proteintech Cat#14909-1-AP; RRID: AB_2180361 Mouse monoclonal anti-Vinculin Santa Cruz Biotechnology Cat#sc-73614; RRID: AB_1131294 Rabbit polyclonal anti-RIOK3 Proteintech Cat#13593-1-AP; RRID: AB_2178105 Rabbit polyclonal anti-Tubulin Cell Signaling Technology Cat#2144; RRID: AB_2210548 Mouse monoclonal anti-FLAG Sigma-Aldrich Cat#A8592; RRID: AB_439702 Anti-mouse IgG Cell Signaling Technology Cat#7076; RRID: AB_330924 Anti-rabbit IgG Cell Signaling Technology Cat#7074; RRID:AB_2099233 Bacterial and virus strains Escherichia coli DH5⍺ Invitrogen Cat#18265017 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (4.5 g/L D-glucose, 584 mg/L L-glutamine, 110 mg/ L sodium pyruvate) ThermoFisher Scientific Cat#11995065 Trypsin-EDTA (0.25%), phenol red ThermoFisher Scientific Cat#25200114 Fetal bovine serum ThermoFisher Scientific Cat#26140079 Fetal bovine serum, dialyzed ThermoFisher Scientific Cat#26400044 DMEM without amino acids USBiological Cat#D9800-27 D-glucose Sigma-Aldrich Cat#G7021 Glycine Sigma-Aldrich Cat#50046 L-Arginine monohydrochloride Sigma-Aldrich Cat#A6969 L-Cystine dihydrochloride Sigma-Aldrich Cat#C6727 L-Glutamine Sigma-Aldrich Cat#G8540 L-Histidine monohydrochloride monohydrate Sigma-Aldrich Cat#H5659 L-Isoleucine Sigma-Aldrich Cat#I7403 L-Leucine Sigma-Aldrich Cat#L8912 L-Lysine monohydrochloride Sigma-Aldrich Cat#L8662 L-Methionine Sigma-Aldrich Cat#M5308 L-Phenylalanine Sigma-Aldrich Cat#P5482 L-Serine Sigma-Aldrich Cat#S4311 L-Threonine Sigma-Aldrich Cat#T8441 L-Tryptophan Sigma-Aldrich Cat#T8941 L-Tyrosine disodium salt hydrate Sigma-Aldrich Cat#T1145 L-Valine Sigma-Aldrich Cat#V0513 Opti-MEM Reduced Serum Media ThermoFisher Scientific Cat#51985034 Lipofectamine 3000 Transfection Reagent Invitrogen Cat#L3000015 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat#13778150 ZymoPURE II Plasmid Midiprep Kit Zymo Research Cat#D4201 (Continued on next page) ll OPEN ACCESS Short article e1 Molecular Cell 86, 1–11.e1–e7, November 19, 2026



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