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Citius Pharmaceuticals biology, microscopy and spectrophotometry services
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Article Title: Stimulation of PGP Bacteria on the Development of Seeds, Plants and Cuttings of the Obligate Halophyte Arthrocaulon (Arthrocnemum) macrostachyum (Moric.) Piirainen & G. Kadereit.
Article Snippet: .. The authors are grateful to University of Seville Greenhouse General Services and Microscopy Service (CITIUS) and Nazaret Fernández Castillo for their collaboration. ..

Article Title: Nodule Synthetic Bacterial Community as Legume Biofertilizer under Abiotic Stress in Estuarine Soils.
Article Snippet: .. Acknowledgments: We thank the personnel from the greenhouse and the microscopy service of the Central Services of the Universidad de Sevilla (CITIUS) for their help. ..

Article Title: Evaluation of rice bran varieties and heat treatment for the development of protein/starch-based bioplastics via injection molding.
Article Snippet: Improper management of disposable plastics and resource depletion pose significant environmental challenges, prompting interest in alternatives like bioplastics.. These novel materials can be produced from by-products of the agro-food industry, offering solutions and valorizing waste.. Rice bran, a substantial by-product of rice processing, is abundant and cost-effective, rich in proteins and starch.

Article Title: A Thermoresponsive injectable drug delivery system of chitosan/β-glycerophosphate with gellan gum/alginate microparticles.
Article Snippet: The development of new Drug Delivery Systems (DDS) by incorporating microparticles within hydrogels can prolong the release rate of drugs and/or other bioactive agents.. In this study, we combined gellan gum/alginate microparticles within a thermoresponsive chitosan (Ch) hydrogel with β-Glycerophosphate (β-GP), designing the system to be in the sol state at 21 C and in the gel state at 37 C to enable the injectability of the system.. The system was in the sol state between 10 C and 21 C. Higher concentrations of β-GP (0, 2, 3, 4, 5 w/v%) and microparticles (0, 2 and 5 w/v%) allowed a faster sol-gel transition with higher mechanical strength at 37 C. However, the sol-gel transition was not instantaneous.

Article Title: Effects of the green cross-linking agent tannic acid and its oxidation on the properties of porcine plasma protein superabsorbent materials.
Article Snippet: Tannic acid is a natural polyphenol capable of strongly interacting with proteins, with good antioxidant and antibacterial properties.. Thus, tannic acid (TA) or oxidized tannic acid (oxTA) may be used as cross-linking agents in the development of reinforced and fully protein-based superabsorbent materials (SAMs). oxTA was produced so that reactive quinone groups were generated, which are expected to increase its reactivity.. In this study, porcine plasma protein (PPP) and glycerol (gly) were used in a 50/50 PPP/gly ratio to obtain SAMs through twin screw mixing and injection molding.

Article Title: Effects of combined CBGA and cannabis-derived terpene nanoformulations on TRPV1 activation: Implications for enhanced pain management.
Article Snippet: .. The authors especially thank Biology, Microscopy and Spectrophotometry Services from Centro de Investigations, Tecnología e Innovación (CITIUS, Universidad de Seville) for their technical assistance. ..

other:

Article Title: Synergistic Antibacterial Effects of Amoxicillin and Gold Nanoparticles: A Therapeutic Option to Combat Antibiotic Resistance.
Article Snippet: Acknowledgments: The authors are grateful to the Microscopy, Herbarium and Functional Characterization Service of the Research, Technology, and Innovation Center of the University of Seville (CITIUS).

Article Title: Au@16-pH-16/miR-21 mimic nanosystem: An efficient treatment for obesity through browning and thermogenesis induction.
Article Snippet: The authors are grateful to the Microscopy and Functional Characterization Service of the Research, Technology and Innovation Center of the University of Seville (CITIUS).

Raw Material:

Article Title: Evaluation of rice bran varieties and heat treatment for the development of protein/starch-based bioplastics via injection molding.
Article Snippet: Improper management of disposable plastics and resource depletion pose significant environmental challenges, prompting interest in alternatives like bioplastics.. These novel materials can be produced from by-products of the agro-food industry, offering solutions and valorizing waste.. Rice bran, a substantial by-product of rice processing, is abundant and cost-effective, rich in proteins and starch.

Functional Assay:

Article Title: Effects of the green cross-linking agent tannic acid and its oxidation on the properties of porcine plasma protein superabsorbent materials.
Article Snippet: Tannic acid is a natural polyphenol capable of strongly interacting with proteins, with good antioxidant and antibacterial properties.. Thus, tannic acid (TA) or oxidized tannic acid (oxTA) may be used as cross-linking agents in the development of reinforced and fully protein-based superabsorbent materials (SAMs). oxTA was produced so that reactive quinone groups were generated, which are expected to increase its reactivity.. In this study, porcine plasma protein (PPP) and glycerol (gly) were used in a 50/50 PPP/gly ratio to obtain SAMs through twin screw mixing and injection molding.

Spectrophotometry:

Article Title: Effects of combined CBGA and cannabis-derived terpene nanoformulations on TRPV1 activation: Implications for enhanced pain management.
Article Snippet: .. The authors especially thank Biology, Microscopy and Spectrophotometry Services from Centro de Investigations, Tecnología e Innovación (CITIUS, Universidad de Seville) for their technical assistance. ..



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Arginine inhibits S. Typhimurium infection by interacting with RPS3 (-B) Enrichment analysis of RAW264.7 cells cell lysate and arginine binding protein , pathway (A) and heatmap of protein abundance associated with Salmonella infection ( B, C ) Knockdown efficiency of RPS3 in RAW264.7 cell line was verified by Western blotting ( n = 5 independent experiments). (D) DCFH-DA fluorescent probe assay for detection of cellular ROS Levels ( n = 5 independent experiments). (E) Representative confocal <t>microscopy</t> image illustrating the mitochondrial abundance within macrophages ( n = 5 independent experiments). (F) Venn diagram of RPS3 binding, arginine metabolism, and protein overlap associated with Salmonella infection. (G) Interactions between RPS3 interaction with SRSF3 was analyzed using the GeneMANIA database. (H) RPS3 and SRSF3 by co-immunoprecipitation assays using anti-SRSF3 antibodies. The data are reported as the mean ± SD. **p < 0.01.
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Arginine inhibits S. Typhimurium infection by interacting with RPS3 (-B) Enrichment analysis of RAW264.7 cells cell lysate and arginine binding protein , pathway (A) and heatmap of protein abundance associated with Salmonella infection ( B, C ) Knockdown efficiency of RPS3 in RAW264.7 cell line was verified by Western blotting ( n = 5 independent experiments). (D) DCFH-DA fluorescent probe assay for detection of cellular ROS Levels ( n = 5 independent experiments). (E) Representative confocal microscopy image illustrating the mitochondrial abundance within macrophages ( n = 5 independent experiments). (F) Venn diagram of RPS3 binding, arginine metabolism, and protein overlap associated with Salmonella infection. (G) Interactions between RPS3 interaction with SRSF3 was analyzed using the GeneMANIA database. (H) RPS3 and SRSF3 by co-immunoprecipitation assays using anti-SRSF3 antibodies. The data are reported as the mean ± SD. **p < 0.01.

Journal: Frontiers in Immunology

Article Title: Arginine-mediated inhibition of macrophage apoptosis by Escherichia coli nissle 1917 in Salmonella typhimurium-induced intestinal inflammation

doi: 10.3389/fimmu.2025.1684234

Figure Lengend Snippet: Arginine inhibits S. Typhimurium infection by interacting with RPS3 (-B) Enrichment analysis of RAW264.7 cells cell lysate and arginine binding protein , pathway (A) and heatmap of protein abundance associated with Salmonella infection ( B, C ) Knockdown efficiency of RPS3 in RAW264.7 cell line was verified by Western blotting ( n = 5 independent experiments). (D) DCFH-DA fluorescent probe assay for detection of cellular ROS Levels ( n = 5 independent experiments). (E) Representative confocal microscopy image illustrating the mitochondrial abundance within macrophages ( n = 5 independent experiments). (F) Venn diagram of RPS3 binding, arginine metabolism, and protein overlap associated with Salmonella infection. (G) Interactions between RPS3 interaction with SRSF3 was analyzed using the GeneMANIA database. (H) RPS3 and SRSF3 by co-immunoprecipitation assays using anti-SRSF3 antibodies. The data are reported as the mean ± SD. **p < 0.01.

Article Snippet: Cellular collectives were fixed with 2.5% glutaraldehyde for 24 h, embedded in 2% agarose, and processed by the Electron Microscopy Research Service of China Pharmaceutical University.

Techniques: Infection, Binding Assay, Quantitative Proteomics, Knockdown, Western Blot, Confocal Microscopy, Immunoprecipitation