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Tokai Hit Co Ltd heated microscope stage
Heated Microscope Stage, supplied by Tokai Hit Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+heating+stage/heated+microscope+stage/pm41453368-237-17-20
Average 86 stars, based on 1 article reviews
heated microscope stage - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Imaging:

Article Title: Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair.
Article Snippet: In short, MEFs (1.5 3 105 cells/sample) were suspended in 2 mL of 1 mg/mL rat tail collagen I (Corning) and added to a 24-well plate in quadruplets (500 mL/well). .. Time-lapse imaging of live cells was performed in phenol red-free DMEM media supplemented with 25 mM HEPES and 20% FBS at 37 C and at 85% humidity with a heated stage-top incubator and an objective heater (Tokai Hit). .. Cells were cultured for 24 hrs at 37 C, 5%CO2 and images of the collagen plugs were acquired using a stereo microscope (Leica Microsystems).


Microscopy:

Article Title: Actin and vimentin jointly control cell viscoelasticity and compression stiffening
Article Snippet: The mechanical properties of cells are governed by the cytoskeleton, a dynamic network of actin filaments, intermediate filaments, and microtubules.. Understanding the individual and collective mechanical contributions of these three different cytoskeletal elements is essential to elucidate how cells maintain mechanical integrity during deformation.. Here we use a custom single-cell rheometer to identify the distinct contributions of actin and vimentin to the viscoelastic and nonlinear elastic response of cells to uniaxial compression.

Article Title: Analyzing Spatial Variations in Molecular Mobility in Hydrated Amorphous Drug-Polymer Blends Using Fourier Transform Fluorescence Recovery After Photobleaching and Image Segmentation.
Article Snippet: Amorphous solid dispersions (ASDs) can enhance oral bioavailability of poorly soluble drugs but often undergo hydration-induced phase separation, which complicates drug release and undermines physical stability.. Here we integrate diffractiveoptics−enabled Fourier transform fluorescence recovery after photobleaching (FT-FRAP) with image segmentation in a controlled “glass-sandwich” hydration platform to quantify phase-resolved molecular mobility within hydrating ibuprofen−copovidone compacts.. Release studies distinguished a rapidly releasing 10% w/w drug loading (DL) ibuprofen ASD from a poorly releasing 15% DL ASD.

Article Title: Quorum sensing regulates a polysaccharide biosynthesis gene to control cell aggregation in <i>Paracoccus denitrificans</i>
Article Snippet: We then infused media using a syringe pump (Harvard Apparatus, Harvard MA, USA) at a flow rate of 100 μL h-1. .. The devices were incubated at 30 oC using a heated microscope stage insert (Tokai hit, Japan). .. Sequential images were taken on an inverted microscope (Axio Observer.Z1, Carl Zeiss, Germany) with a 100x oil immersion objective (NA1.4).


Article Title: TRPV1 in arteries enables a rapid myogenic tone
Article Snippet: ASM cells and arteries were respectively loaded with 5 μM and 10 μM Fluo-4-AM (Invitrogen, Thermo Fisher Scientific) in a buffer solution containing 140 mM NaCl, 4 mM KCl, 1 mM MgCl2, 1.2 mM CaCl2, 10 mM HEPES, and 5 mM glucose (pH 7.3). .. Temperature was maintained at 32–33°C using a heated microscope stage (Tokai Hit). .. The bath temperature was verified by a thermistor probe (Warner instruments).

Article Title: Automated oocyte retrieval, denudation, sperm preparation, and ICSI in the IVF laboratory: a proof-of-concept study and report of the first live births.
Article Snippet: STUDY QUESTION: Can multiple automated systems sequentially perform Day 0 IVF procedures: (i) sperm preparation, (ii) cumulus– oocyte complex (COC) retrieval and oocyte denudation, and (iii) ICSI?. SUMMARY ANSWER: Automated sequential Day 0 procedures achieved fertilization in 64.3% of injected oocytes and 42.2% usable blastocyst formation in 11 cases, resulting in five healthy live births from nine patients with positive pregnancy tests.. WHAT IS KNOWN ALREADY: The Day 0 procedures—sperm preparation, oocyte handling (e.g. finding and denudation), and ICSI— rely on embryologist skill.

other:

Article Title: Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair.
Article Snippet: For experiments that require siRNA depletion or protein overexpression, cells were transfected using Nucleofector 2b (Lonza Bioscience) with a house made transfection solution (5 mM KCl, 15 mM MgCl2, 120 mM Na2HPO4 pH 7.2, 50 mM mannitol) and program T-020.

Incubation:

Article Title: Quorum sensing regulates a polysaccharide biosynthesis gene to control cell aggregation in <i>Paracoccus denitrificans</i>
Article Snippet: We then infused media using a syringe pump (Harvard Apparatus, Harvard MA, USA) at a flow rate of 100 μL h-1. .. The devices were incubated at 30 oC using a heated microscope stage insert (Tokai hit, Japan). .. Sequential images were taken on an inverted microscope (Axio Observer.Z1, Carl Zeiss, Germany) with a 100x oil immersion objective (NA1.4).


Control:




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