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microrna profile microarray  (Agilent technologies)


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    Structured Review

    Agilent technologies microrna profile microarray
    a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The <t>microRNA</t> profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.
    Microrna Profile Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microrna+microarray/pmc11133402-143-2-3
    Average 90 stars, based on 1 article reviews
    microrna profile microarray - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression"

    Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-024-06744-0

    a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.
    Figure Legend Snippet: a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

    Techniques Used: Over Expression, Western Blot, Expressing, Rescue Assay, Inhibition

    Related Articles

    Microarray:

    Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression
    Article Snippet: .. Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression. ..

    Article Title: Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology
    Article Snippet: .. Five commercially available microRNA microarray platforms; Agilent Technologies (Santa Clara, CA), Ambion (Austin, TX), Exiqon (Vedbaek, Denmark), Invitrogen (Carlsbad, CA), and Toray (Tokyo, Japan) were tested in this study. ..

    Article Title: Pre-processing and differential expression analysis of Agilent microRNA arrays using the AgiMicroRna Bioconductor library
    Article Snippet: .. Pradervand et al. [ ] carried out a similar investigation using the Agilent microRNA microarray platform and the summarized TGS obtained with the AFE image analysis software provided by the vendor. ..

    Article Title: Pre-processing and differential expression analysis of Agilent microRNA arrays using the AgiMicroRna Bioconductor library
    Article Snippet: .. For each microRNA, the Agilent microRNA microarray platform makes measurements with a number of different oligonucleotide probes that are replicated a number of times across the array surface. .. These replicate signals are summarized into a total gene signal (TGS) with the proprietary Agilent Feature Extraction (AFE) image analysis algorithm [ ], which makes use of the background corrected signals.

    Article Title: Clear cell renal cell carcinoma associated microRNA expression signatures identified by an integrated bioinformatics analysis
    Article Snippet: .. GSE12105 , [ ] , Agilent Human microRNA Microarray , 490 , 12 , 12 , t-test. .. GSE16441 , [ ] , Agilent Human microRNA Microarray , 851 , 8 , 8 , SAM.

    Article Title: Clear cell renal cell carcinoma associated microRNA expression signatures identified by an integrated bioinformatics analysis
    Article Snippet: GSE12105 , [ ] , Agilent Human microRNA Microarray , 490 , 12 , 12 , t-test. .. GSE16441 , [ ] , Agilent Human microRNA Microarray , 851 , 8 , 8 , SAM. ..

    Article Title: MicroRNA-34a modulates genes involved in cellular motility and oxidative phosphorylation in neural precursors derived from human umbilical cord mesenchymal stem cells
    Article Snippet: .. We subjected WJ-MSCs into neurogenesis by a published protocol, and the miRNome patterns of WJ-MSCs and their neuronal progenitors (day 9 after differentiation) were analyzed by the Agilent microRNA microarray. ..

    Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression.
    Article Snippet: .. Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression. ..

    Over Expression:

    Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression
    Article Snippet: .. Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression. ..

    Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression.
    Article Snippet: .. Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression. ..

    Software:

    Article Title: Pre-processing and differential expression analysis of Agilent microRNA arrays using the AgiMicroRna Bioconductor library
    Article Snippet: .. Pradervand et al. [ ] carried out a similar investigation using the Agilent microRNA microarray platform and the summarized TGS obtained with the AFE image analysis software provided by the vendor. ..



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    Image Search Results


    a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

    Journal: Cell Death & Disease

    Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression

    doi: 10.1038/s41419-024-06744-0

    Figure Lengend Snippet: a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

    Article Snippet: Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression.

    Techniques: Over Expression, Western Blot, Expressing, Rescue Assay, Inhibition

    (A) Flowchart of candidate miRNA selection. Microarray results were compared between patients with and without subclinical tumors or recurrence under or after adjuvant chemotherapy, and candidate miRNAs were isolated. (B) Volcano plots for candidate miRNAs in patients with recurrence during AC. ( C)(D) GSEA comparing Panc1-GR and Panc1-Pt cells. GSEA-extracted representative gene sets enriched in these cells are shown. ( E) List showing the ranking of candidate miRNAs from the above 5 miRNAs according to the percentage of genes related to the integrin-mediated cell adhesion pathway retrieved by TargetScan.

    Journal: PLOS ONE

    Article Title: MicroRNA-26a-5p is a reliable biomarker in the adjuvant setting for pancreatic ductal adenocarcinoma

    doi: 10.1371/journal.pone.0310328

    Figure Lengend Snippet: (A) Flowchart of candidate miRNA selection. Microarray results were compared between patients with and without subclinical tumors or recurrence under or after adjuvant chemotherapy, and candidate miRNAs were isolated. (B) Volcano plots for candidate miRNAs in patients with recurrence during AC. ( C)(D) GSEA comparing Panc1-GR and Panc1-Pt cells. GSEA-extracted representative gene sets enriched in these cells are shown. ( E) List showing the ranking of candidate miRNAs from the above 5 miRNAs according to the percentage of genes related to the integrin-mediated cell adhesion pathway retrieved by TargetScan.

    Article Snippet: To investigate candidate miRNAs, microarray analyses of miRNA expression in 18 blood samples ( ) was performed using the Toray microRNA microarray system (Toray Industries, Tokyo, Japan).

    Techniques: Selection, Microarray, Adjuvant, Isolation