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LC Sciences microrna detection microarray service

Quantitative PCR validates miR-21 <t>microarray</t> results. Two first columns compare the averaged fold change between 17 dpa blastema (Bl) and stump (St) samples for LNA based qPCR assays (yellow bar), and for previous microarray data (red bar). Also, the individual fold changes between Bl and St for the three animals (biological replicates) are shown (blue bars). The relative miR-21 expression was calculated based on the efficiency corrected ΔΔCt method and normalized with miR-20a and miR-200b . The y-axis is a log scale. A fold change >1 indicates upregulation in Bl compared to St samples [ p ≤0.03 (qPCR), p ≤0.0001 (array); two-tailed t- test]. Inset , illustrates how non-isotopic Northern blot using digoxigenin-labeled LNAs against hsa-miR21 and hsa-U6 (control) are useful to detect the axolotl versions of these small RNAs. The axolotl miR-21 ( Amex-miR-21 ) is detected as a band of ∼20 nt which is over expressed in blastema when compared with stump and blood samples.

Microrna Detection Microarray Service, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microrna+microarray+services/mirna+microarray/pmc03441534-123-26-17
Average 90 stars, based on 1 article reviews

microrna detection microarray service - by Bioz Stars, 2026-11

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1) Product Images from "Microarray Analysis of microRNA Expression during Axolotl Limb Regeneration"

Article Title: Microarray Analysis of microRNA Expression during Axolotl Limb Regeneration

Journal: PLoS ONE

doi: 10.1371/journal.pone.0041804

Quantitative PCR validates miR-21 microarray results. Two first columns compare the averaged fold change between 17 dpa blastema (Bl) and stump (St) samples for LNA based qPCR assays (yellow bar), and for previous microarray data (red bar). Also, the individual fold changes between Bl and St for the three animals (biological replicates) are shown (blue bars). The relative miR-21 expression was calculated based on the efficiency corrected ΔΔCt method and normalized with miR-20a and miR-200b . The y-axis is a log scale. A fold change >1 indicates upregulation in Bl compared to St samples [ p ≤0.03 (qPCR), p ≤0.0001 (array); two-tailed t- test]. Inset , illustrates how non-isotopic Northern blot using digoxigenin-labeled LNAs against hsa-miR21 and hsa-U6 (control) are useful to detect the axolotl versions of these small RNAs. The axolotl miR-21 ( Amex-miR-21 ) is detected as a band of ∼20 nt which is over expressed in blastema when compared with stump and blood samples.

Figure Legend Snippet: Quantitative PCR validates miR-21 microarray results. Two first columns compare the averaged fold change between 17 dpa blastema (Bl) and stump (St) samples for LNA based qPCR assays (yellow bar), and for previous microarray data (red bar). Also, the individual fold changes between Bl and St for the three animals (biological replicates) are shown (blue bars). The relative miR-21 expression was calculated based on the efficiency corrected ΔΔCt method and normalized with miR-20a and miR-200b . The y-axis is a log scale. A fold change >1 indicates upregulation in Bl compared to St samples [ p ≤0.03 (qPCR), p ≤0.0001 (array); two-tailed t- test]. Inset , illustrates how non-isotopic Northern blot using digoxigenin-labeled LNAs against hsa-miR21 and hsa-U6 (control) are useful to detect the axolotl versions of these small RNAs. The axolotl miR-21 ( Amex-miR-21 ) is detected as a band of ∼20 nt which is over expressed in blastema when compared with stump and blood samples.

Techniques Used: Real-time Polymerase Chain Reaction, Microarray, Expressing, Two Tailed Test, Non-Isotopic Labeling, Northern Blot, Labeling, Control

A. Comparison between the human and axolotl miR-21 target sites in their Jagged1 genes. Nucleotide alignment of the miR-21 target site-containing region present in the 3′-UTR of the human Jagged1 ( Hsa-Jag1 , NM_000214) and the axolotl Jagged1 ( Amx-Jag1 , JF907581). The 22 bases comprising the target site for miR-21 are in yellow except for the 7 bases complementary to the miR-21 seed region (green color). Vertical bars (|) denote nucleotide identities between the two sequences. The numbers at both sides of the alignment are the nucleotide position on each 3′-UTR. B. In vitro luciferase assays testing the effect of miR-21 on the 3′-UTR of Amex-Jag1 in axolotl cells . Results are expressed as percent of co-reporter-normalized Renilla activity against reference vectors. Bars denote standard error of mean of the amount of independent assays. Student t- test was done and the obtained p- values determined that the Target of Hsa-miR-21 is a good positive control as biosensor for the activity of this microRNA ( p ≤0.005; two-tailed t- test). These results suggest that Amex-Jag1 may in fact be a target for miR-21 because significant differences were found in the Renilla signal recorded from cells electroporated with only the vector containing the 3′-UTR of Amex-Jag1 , versus the cells that were also electroporated with Pre-miR-21 *** (p≤0.005) but not Pre-miR30a. When the latter experiment was repeated with the mutant, seedless - Amex-Jag1- 3′-UTR, any sensitivity to exogenous miR-21 was lost.

Figure Legend Snippet: A. Comparison between the human and axolotl miR-21 target sites in their Jagged1 genes. Nucleotide alignment of the miR-21 target site-containing region present in the 3′-UTR of the human Jagged1 ( Hsa-Jag1 , NM_000214) and the axolotl Jagged1 ( Amx-Jag1 , JF907581). The 22 bases comprising the target site for miR-21 are in yellow except for the 7 bases complementary to the miR-21 seed region (green color). Vertical bars (|) denote nucleotide identities between the two sequences. The numbers at both sides of the alignment are the nucleotide position on each 3′-UTR. B. In vitro luciferase assays testing the effect of miR-21 on the 3′-UTR of Amex-Jag1 in axolotl cells . Results are expressed as percent of co-reporter-normalized Renilla activity against reference vectors. Bars denote standard error of mean of the amount of independent assays. Student t- test was done and the obtained p- values determined that the Target of Hsa-miR-21 is a good positive control as biosensor for the activity of this microRNA ( p ≤0.005; two-tailed t- test). These results suggest that Amex-Jag1 may in fact be a target for miR-21 because significant differences were found in the Renilla signal recorded from cells electroporated with only the vector containing the 3′-UTR of Amex-Jag1 , versus the cells that were also electroporated with Pre-miR-21 *** (p≤0.005) but not Pre-miR30a. When the latter experiment was repeated with the mutant, seedless - Amex-Jag1- 3′-UTR, any sensitivity to exogenous miR-21 was lost.

Techniques Used: Comparison, In Vitro, Luciferase, Activity Assay, Positive Control, Two Tailed Test, Plasmid Preparation, Mutagenesis

Microarray:

Article Title: Thymosin Beta-4 Modulates Cardiac Remodeling by Regulating ROCK1 Expression in Adult Mammals.
Article Snippet: Following three washing steps in TBS for 5 min, nuclei were counterstained with DAPI (1 uM) (Thermo Fisher Scientific, Waltham, MA, USA), rinsed in TBS and protected by antifade reagent (Slow fade anti-fade Kit, Invitrogen, Carlsbad, CA, USA; S2828), covered via glass coverslips and documented by Zeiss LSM-710 confocal microscopy. .. For microRNA microarrays, statistical tests and clustering analyses were provided by LC Sciences as part of the miRNA microarray service. ..

Article Title: Monolayer culture alters EGFR inhibitor response through abrogation of microRNA-mediated feedback regulation
Article Snippet: .. The microRNA microarray was performed by LC Sciences (Houston, TX; RRID:SCR_000140) using μParafloTM chip technology with probes for all miRNA listed in Sanger miRBase Release 21 (mirBase.org, RRID:SCR_003152)). .. miR-146a-5p was quantified from RNA via reverse transcriptase qPCR, using miRCURY LNA miRNA SYBR Green RT-PCR Kit (Qiagen #339345) with miR-146a-5p-specific primers (Qiagen #YP00204688) as per the manufacturer’s instructions.

Article Title: MicroRNA expression profiling of cutaneous squamous cell carcinomas and precursor lesions
Article Snippet: All RNA samples were quantified using Qubit RNA HS Assay kit (Invitrogen, #Q32852) as per manufacturer's protocol. .. A total of 500 ng Total RNA from normal skin ( n = 4), perilesional ( n = 4), AK ( n = 10), IEC ( n = 10) and SCC ( n = 10) were shipped to LC Sciences (Houston, USA) to perform the miRNA microarray profiling. ..

High Throughput Screening Assay:

Article Title: Research progress in high-throughput DNA synthesis and its applications.
Article Snippet: In recent years, the development of high-throughput DNA synthesis technology has significantly advanced research in genomics and synthetic biology.. Traditional DNA synthesis methods, such as firstgeneration DNA synthesizer and PCR-based approaches, have demonstrated excellent performance in many aspects.. However, they exhibit notable limitations in de novo synthesis of long-chain DNA and large-scale parallel synthesis.

Expressing:

Hybridization:

Article Title: miRNAs mediate the impact of smoking on dental pulp stem cells via the p53 pathway.
Article Snippet: Cigarette smoke changes the genomic and epigenomic imprint of cells.. In this study, we investigated the biological consequences of extended cigarette smoke exposure on dental pulp stem cells (DPSCs) and the potential roles of miRNAs.. DPSCs were treated with various doses of cigarette smoke condensate (CSC) for up to 6 weeks.

other:

Article Title: Integrated Analysis of lncRNA-miRNA-mRNA Regulatory Network in Rapamycin-Induced Cardioprotection against Ischemia/Reperfusion Injury in Diabetic Rabbits.
Article Snippet: The pooled RNA isolated from 3 different biological replicates from the 4 experimental groups was subjected to miRNA-Array analysis by loading on the microRNA array (miR-array) chip (LC Sciences Company, Houston, TX, USA).

Article Title: Feedback enabled synthetic genes, target seed match cassettes, and their uses
Article Snippet: Raw data was processed by LC Sciences according to their technical bulletin (Sciences, L. microRNA Microarray Data Analysis).

Isolation:

Article Title: Integrated Analysis of lncRNA-miRNA-mRNA Regulatory Network in Rapamycin-Induced Cardioprotection against Ischemia/Reperfusion Injury in Diabetic Rabbits.
Article Snippet: .. Total RNA isolated from LV tissue of DM, DM + I/R, and DM + I/R + RAPA was subjected to miRNA array analysis (LC Sciences Company, Houston, TX, USA). ..

Image Search Results

Heat map of microRNA expression profiles in the whole ischemic area from sham-operated, tMCAO and tMCAO + RLIP animals. (A) The average of signal intensity values for each significantly expressed miRNA is reported (p < 0.05). Red and green indicate up- and down-regulation respectively, according to the colorimetric scale in the right side of the panel. a, b and c indicate the three experimental group: (a) Sham, (b) tMCAO and (c) tMCAO + RLIP animals. Each vertical line refers to three samples for each experimental group. (B) Volcano plot analysis in the lower side of panel shows the comparison of miRNA levels after tMCAO and RLIP induction. The y-axis corresponds to the mean expression value of log 10 (p-value), and the x-axis displays the log 2 fold change value. The blue dots represent the miRNA levels expressed in rat brain 24 h after stroke induction; the orange dots represent the miRNA levels expressed in rat brain 24 h after tMCAO + RLIP.

Journal: Theranostics

Article Title: Remote postconditioning ameliorates stroke damage by preventing let-7a and miR-143 up-regulation

doi: 10.7150/thno.48135

Figure Lengend Snippet: Heat map of microRNA expression profiles in the whole ischemic area from sham-operated, tMCAO and tMCAO + RLIP animals. (A) The average of signal intensity values for each significantly expressed miRNA is reported (p < 0.05). Red and green indicate up- and down-regulation respectively, according to the colorimetric scale in the right side of the panel. a, b and c indicate the three experimental group: (a) Sham, (b) tMCAO and (c) tMCAO + RLIP animals. Each vertical line refers to three samples for each experimental group. (B) Volcano plot analysis in the lower side of panel shows the comparison of miRNA levels after tMCAO and RLIP induction. The y-axis corresponds to the mean expression value of log 10 (p-value), and the x-axis displays the log 2 fold change value. The blue dots represent the miRNA levels expressed in rat brain 24 h after stroke induction; the orange dots represent the miRNA levels expressed in rat brain 24 h after tMCAO + RLIP.

Article Snippet: RNA samples were sent to LC Sciences (Houston, Texas, USA), a global biotechnology company that is provided with a microRNA microarray service.

Techniques: Expressing, Comparison

Validation of microarray results by real-time PCR. MicroRNA levels analysed by Real-Time PCR in ischemic brain regions from rats subjected to tMCAO and tMCAO + RLIP are expressed as fold change over the respective sham-operated controls. Each column represents the mean ± S.E.M. Results of microRNAs expression were normalized with respect to 4.5S RNA as internal control. n = 3 or 4 per group. S is for Sham-operated group.

Journal: Theranostics

Article Title: Remote postconditioning ameliorates stroke damage by preventing let-7a and miR-143 up-regulation

doi: 10.7150/thno.48135

Figure Lengend Snippet: Validation of microarray results by real-time PCR. MicroRNA levels analysed by Real-Time PCR in ischemic brain regions from rats subjected to tMCAO and tMCAO + RLIP are expressed as fold change over the respective sham-operated controls. Each column represents the mean ± S.E.M. Results of microRNAs expression were normalized with respect to 4.5S RNA as internal control. n = 3 or 4 per group. S is for Sham-operated group.

Article Snippet: RNA samples were sent to LC Sciences (Houston, Texas, USA), a global biotechnology company that is provided with a microRNA microarray service.

Techniques: Biomarker Discovery, Microarray, Real-time Polymerase Chain Reaction, Expressing, Control

Journal: PLoS ONE

Article Title: Microarray Analysis of microRNA Expression during Axolotl Limb Regeneration

doi: 10.1371/journal.pone.0041804

Figure Lengend Snippet: Quantitative PCR validates miR-21 microarray results. Two first columns compare the averaged fold change between 17 dpa blastema (Bl) and stump (St) samples for LNA based qPCR assays (yellow bar), and for previous microarray data (red bar). Also, the individual fold changes between Bl and St for the three animals (biological replicates) are shown (blue bars). The relative miR-21 expression was calculated based on the efficiency corrected ΔΔCt method and normalized with miR-20a and miR-200b . The y-axis is a log scale. A fold change >1 indicates upregulation in Bl compared to St samples [ p ≤0.03 (qPCR), p ≤0.0001 (array); two-tailed t- test]. Inset , illustrates how non-isotopic Northern blot using digoxigenin-labeled LNAs against hsa-miR21 and hsa-U6 (control) are useful to detect the axolotl versions of these small RNAs. The axolotl miR-21 ( Amex-miR-21 ) is detected as a band of ∼20 nt which is over expressed in blastema when compared with stump and blood samples.

Article Snippet: After running the RNA samples on a gel and confirming their integrity, 1.2 μg were sent to LC Sciences (Houston, TX) to be processed using their MicroRNA detection Microarray Service, and to be hybridized to a custom vertebrates chip (MRA-2001).

Techniques: Real-time Polymerase Chain Reaction, Microarray, Expressing, Two Tailed Test, Non-Isotopic Labeling, Northern Blot, Labeling, Control

Journal: PLoS ONE

Article Title: Microarray Analysis of microRNA Expression during Axolotl Limb Regeneration

doi: 10.1371/journal.pone.0041804

Figure Lengend Snippet: A. Comparison between the human and axolotl miR-21 target sites in their Jagged1 genes. Nucleotide alignment of the miR-21 target site-containing region present in the 3′-UTR of the human Jagged1 ( Hsa-Jag1 , NM_000214) and the axolotl Jagged1 ( Amx-Jag1 , JF907581). The 22 bases comprising the target site for miR-21 are in yellow except for the 7 bases complementary to the miR-21 seed region (green color). Vertical bars (|) denote nucleotide identities between the two sequences. The numbers at both sides of the alignment are the nucleotide position on each 3′-UTR. B. In vitro luciferase assays testing the effect of miR-21 on the 3′-UTR of Amex-Jag1 in axolotl cells . Results are expressed as percent of co-reporter-normalized Renilla activity against reference vectors. Bars denote standard error of mean of the amount of independent assays. Student t- test was done and the obtained p- values determined that the Target of Hsa-miR-21 is a good positive control as biosensor for the activity of this microRNA ( p ≤0.005; two-tailed t- test). These results suggest that Amex-Jag1 may in fact be a target for miR-21 because significant differences were found in the Renilla signal recorded from cells electroporated with only the vector containing the 3′-UTR of Amex-Jag1 , versus the cells that were also electroporated with Pre-miR-21 *** (p≤0.005) but not Pre-miR30a. When the latter experiment was repeated with the mutant, seedless - Amex-Jag1- 3′-UTR, any sensitivity to exogenous miR-21 was lost.

Techniques: Comparison, In Vitro, Luciferase, Activity Assay, Positive Control, Two Tailed Test, Plasmid Preparation, Mutagenesis

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