gene exp npm1 hs02339479 g1 (Thermo Fisher)
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gene exp npm1 hs02339479 g1
Gene Exp Npm1 Hs02339479 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microrna+microarray+gene+expression+experiments/Gene+Exp%2E+NPM1%2C+Hs02339479_g1/pm35045331-289-57-63
Average 86 stars, based on 1 article reviews
Gene Exp Npm1 Hs02339479 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microrna+microarray+gene+expression+experiments/Gene+Exp%2E+NPM1%2C+Hs02339479_g1/pm35045331-289-57-63
Average 86 stars, based on 1 article reviews
gene exp npm1 hs02339479 g1 - by Bioz Stars,
2026-11
86/100 stars
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other:Article Title: Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus Article Snippet: NPM1 , Nucleophosmin/protein B23) , Hs02339479_g1. Reverse Transcription Polymerase Chain Reaction:Article Title: TGS1 mediates mRNA 5’-cap trimethylation to promote oxidative phosphorylation in acute myeloid leukaemia Article Snippet: .. Spleens were analysed by RT-PCR to evaluate human NPM expression (Taqman Expressing:Article Title: TGS1 mediates mRNA 5’-cap trimethylation to promote oxidative phosphorylation in acute myeloid leukaemia Article Snippet: .. Spleens were analysed by RT-PCR to evaluate human NPM expression (Taqman Transfection:Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 5-Bromo-2-deoxyuridine (BrdU) Sigma Cat# B5002 10058-F4 Sigma Cat# F3680 CU-T12-9 Tocris Bioscience Cat# 5414 Cycloheximide Santa Cruz Cat# sc-3508 Methocult GF M3434 Stem Cell Technologies Cat# 03434 Mouse Flt3-ligand cytokine PeproTech Cat# 250-31 Human IL-6 cytokine PeproTech Cat# 200-06 Mouse SCF cytokine PeproTech Cat# 250-03 Human TPO cytokine PeproTech Cat# 300-18 RPMI Fisher Cat# SH30027.01 DMEM Fisher Cat# SH30022FS Fetal Bovine Serum Atlanta Biologicals Cat# S11550 Penicillin/Steptomycin ThermoFisher Cat# SV30010 TransIT-LT1 transfection reagent Mirus Cat# MIR2306 IMDM Corning Cat# 10-016-CV Benzonase Millipore Cat# 70746 Nitrocellulose membrane Bio-Rad Cat# 162-0112 RIPA Lysis Buffer Santa Cruz Cat# sc-24948 Protein A/G Plus beads-Agarose Santa Cruz Cat# sc-2003 TrueCut Cas9 Protein v2 Thermo Fisher Cat# A36498 SuperScript II Reverse Transcriptase Invitrogen Cat# 18064014 TaqMan Universal PCR Master Mix Life Technologies Cat# 4324018 Recombinant c-Myc (WT) Abcam Cat# ab169901 Recombinant c-Myc (K148R) GeneScript N/A Recombinant TRAF6 Life Sensors Cat# UB312 Polybrene Millipore Cat# TR-1003-G EcoRI-HF New England Biolabs Cat# R3101L XhoI New England Biolabs Cat# R0146S XbaI New England Biolabs Cat# R0145S Quick Ligation kit New England Biolabs Cat# M2200S Puromycin Corning Cat# 61-385-RA Doxycycline Fisher Scientific Cat# BP26535 Poly(I:C) Tocris Bioscience Cat# 4287 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 5-fluorouracil (5-FU) CCHMC pharmacy NDC#63323-117-10 2-mercaptoethanol Fisher Cat#03446I Blasticidin Gibco Cat# R21001 RetroNectin Takara Cat# T100B AMPURE XP 60ML Thermo Fisher Scientific Cat#NC9933872 FORMALDEHYDE 20% 10X10ML Thermo Fisher Scientific Cat# NC9611804 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 559619 CellTiterGlo kit Promega Cat# G7570 Zymo Quick-RNA Miniprep Kit Zymo Research Cat# R1050 Mouse hematopoietic progenitor cell enrichment kit Stem Cell Technologies Cat# 19856 Nuclear Extract Kit Active Motif Cat# 40010 BCA assay Pierce Cat# 32106 (Continued on next page) e2 Cell Stem Cell 29, 298–314.e1–e9, February 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neon Transfection System 10 mL Kit Invitrogen Cat# MPK1025 RNeasy Plus Micro Kit Qiagen Cat# 74034 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio Cat# 634899 Taqman Assay human CDK4 probe Thermo Fisher Cat# 4453320 Assay ID: Hs00364847_m1 Taqman Assay Human NPM1 probe Thermo Fisher Cat# 4448892 Assay ID: Sequencing:Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 5-Bromo-2-deoxyuridine (BrdU) Sigma Cat# B5002 10058-F4 Sigma Cat# F3680 CU-T12-9 Tocris Bioscience Cat# 5414 Cycloheximide Santa Cruz Cat# sc-3508 Methocult GF M3434 Stem Cell Technologies Cat# 03434 Mouse Flt3-ligand cytokine PeproTech Cat# 250-31 Human IL-6 cytokine PeproTech Cat# 200-06 Mouse SCF cytokine PeproTech Cat# 250-03 Human TPO cytokine PeproTech Cat# 300-18 RPMI Fisher Cat# SH30027.01 DMEM Fisher Cat# SH30022FS Fetal Bovine Serum Atlanta Biologicals Cat# S11550 Penicillin/Steptomycin ThermoFisher Cat# SV30010 TransIT-LT1 transfection reagent Mirus Cat# MIR2306 IMDM Corning Cat# 10-016-CV Benzonase Millipore Cat# 70746 Nitrocellulose membrane Bio-Rad Cat# 162-0112 RIPA Lysis Buffer Santa Cruz Cat# sc-24948 Protein A/G Plus beads-Agarose Santa Cruz Cat# sc-2003 TrueCut Cas9 Protein v2 Thermo Fisher Cat# A36498 SuperScript II Reverse Transcriptase Invitrogen Cat# 18064014 TaqMan Universal PCR Master Mix Life Technologies Cat# 4324018 Recombinant c-Myc (WT) Abcam Cat# ab169901 Recombinant c-Myc (K148R) GeneScript N/A Recombinant TRAF6 Life Sensors Cat# UB312 Polybrene Millipore Cat# TR-1003-G EcoRI-HF New England Biolabs Cat# R3101L XhoI New England Biolabs Cat# R0146S XbaI New England Biolabs Cat# R0145S Quick Ligation kit New England Biolabs Cat# M2200S Puromycin Corning Cat# 61-385-RA Doxycycline Fisher Scientific Cat# BP26535 Poly(I:C) Tocris Bioscience Cat# 4287 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 5-fluorouracil (5-FU) CCHMC pharmacy NDC#63323-117-10 2-mercaptoethanol Fisher Cat#03446I Blasticidin Gibco Cat# R21001 RetroNectin Takara Cat# T100B AMPURE XP 60ML Thermo Fisher Scientific Cat#NC9933872 FORMALDEHYDE 20% 10X10ML Thermo Fisher Scientific Cat# NC9611804 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 559619 CellTiterGlo kit Promega Cat# G7570 Zymo Quick-RNA Miniprep Kit Zymo Research Cat# R1050 Mouse hematopoietic progenitor cell enrichment kit Stem Cell Technologies Cat# 19856 Nuclear Extract Kit Active Motif Cat# 40010 BCA assay Pierce Cat# 32106 (Continued on next page) e2 Cell Stem Cell 29, 298–314.e1–e9, February 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neon Transfection System 10 mL Kit Invitrogen Cat# MPK1025 RNeasy Plus Micro Kit Qiagen Cat# 74034 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio Cat# 634899 Taqman Assay human CDK4 probe Thermo Fisher Cat# 4453320 Assay ID: Hs00364847_m1 Taqman Assay Human NPM1 probe Thermo Fisher Cat# 4448892 Assay ID: TaqMan Assay:Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 5-Bromo-2-deoxyuridine (BrdU) Sigma Cat# B5002 10058-F4 Sigma Cat# F3680 CU-T12-9 Tocris Bioscience Cat# 5414 Cycloheximide Santa Cruz Cat# sc-3508 Methocult GF M3434 Stem Cell Technologies Cat# 03434 Mouse Flt3-ligand cytokine PeproTech Cat# 250-31 Human IL-6 cytokine PeproTech Cat# 200-06 Mouse SCF cytokine PeproTech Cat# 250-03 Human TPO cytokine PeproTech Cat# 300-18 RPMI Fisher Cat# SH30027.01 DMEM Fisher Cat# SH30022FS Fetal Bovine Serum Atlanta Biologicals Cat# S11550 Penicillin/Steptomycin ThermoFisher Cat# SV30010 TransIT-LT1 transfection reagent Mirus Cat# MIR2306 IMDM Corning Cat# 10-016-CV Benzonase Millipore Cat# 70746 Nitrocellulose membrane Bio-Rad Cat# 162-0112 RIPA Lysis Buffer Santa Cruz Cat# sc-24948 Protein A/G Plus beads-Agarose Santa Cruz Cat# sc-2003 TrueCut Cas9 Protein v2 Thermo Fisher Cat# A36498 SuperScript II Reverse Transcriptase Invitrogen Cat# 18064014 TaqMan Universal PCR Master Mix Life Technologies Cat# 4324018 Recombinant c-Myc (WT) Abcam Cat# ab169901 Recombinant c-Myc (K148R) GeneScript N/A Recombinant TRAF6 Life Sensors Cat# UB312 Polybrene Millipore Cat# TR-1003-G EcoRI-HF New England Biolabs Cat# R3101L XhoI New England Biolabs Cat# R0146S XbaI New England Biolabs Cat# R0145S Quick Ligation kit New England Biolabs Cat# M2200S Puromycin Corning Cat# 61-385-RA Doxycycline Fisher Scientific Cat# BP26535 Poly(I:C) Tocris Bioscience Cat# 4287 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 5-fluorouracil (5-FU) CCHMC pharmacy NDC#63323-117-10 2-mercaptoethanol Fisher Cat#03446I Blasticidin Gibco Cat# R21001 RetroNectin Takara Cat# T100B AMPURE XP 60ML Thermo Fisher Scientific Cat#NC9933872 FORMALDEHYDE 20% 10X10ML Thermo Fisher Scientific Cat# NC9611804 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 559619 CellTiterGlo kit Promega Cat# G7570 Zymo Quick-RNA Miniprep Kit Zymo Research Cat# R1050 Mouse hematopoietic progenitor cell enrichment kit Stem Cell Technologies Cat# 19856 Nuclear Extract Kit Active Motif Cat# 40010 BCA assay Pierce Cat# 32106 (Continued on next page) e2 Cell Stem Cell 29, 298–314.e1–e9, February 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neon Transfection System 10 mL Kit Invitrogen Cat# MPK1025 RNeasy Plus Micro Kit Qiagen Cat# 74034 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio Cat# 634899 Taqman Assay human CDK4 probe Thermo Fisher Cat# 4453320 Assay ID: Hs00364847_m1 Taqman Assay Human NPM1 probe Thermo Fisher Cat# 4448892 Assay ID: Ubiquitin Proteomics:Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 5-Bromo-2-deoxyuridine (BrdU) Sigma Cat# B5002 10058-F4 Sigma Cat# F3680 CU-T12-9 Tocris Bioscience Cat# 5414 Cycloheximide Santa Cruz Cat# sc-3508 Methocult GF M3434 Stem Cell Technologies Cat# 03434 Mouse Flt3-ligand cytokine PeproTech Cat# 250-31 Human IL-6 cytokine PeproTech Cat# 200-06 Mouse SCF cytokine PeproTech Cat# 250-03 Human TPO cytokine PeproTech Cat# 300-18 RPMI Fisher Cat# SH30027.01 DMEM Fisher Cat# SH30022FS Fetal Bovine Serum Atlanta Biologicals Cat# S11550 Penicillin/Steptomycin ThermoFisher Cat# SV30010 TransIT-LT1 transfection reagent Mirus Cat# MIR2306 IMDM Corning Cat# 10-016-CV Benzonase Millipore Cat# 70746 Nitrocellulose membrane Bio-Rad Cat# 162-0112 RIPA Lysis Buffer Santa Cruz Cat# sc-24948 Protein A/G Plus beads-Agarose Santa Cruz Cat# sc-2003 TrueCut Cas9 Protein v2 Thermo Fisher Cat# A36498 SuperScript II Reverse Transcriptase Invitrogen Cat# 18064014 TaqMan Universal PCR Master Mix Life Technologies Cat# 4324018 Recombinant c-Myc (WT) Abcam Cat# ab169901 Recombinant c-Myc (K148R) GeneScript N/A Recombinant TRAF6 Life Sensors Cat# UB312 Polybrene Millipore Cat# TR-1003-G EcoRI-HF New England Biolabs Cat# R3101L XhoI New England Biolabs Cat# R0146S XbaI New England Biolabs Cat# R0145S Quick Ligation kit New England Biolabs Cat# M2200S Puromycin Corning Cat# 61-385-RA Doxycycline Fisher Scientific Cat# BP26535 Poly(I:C) Tocris Bioscience Cat# 4287 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 5-fluorouracil (5-FU) CCHMC pharmacy NDC#63323-117-10 2-mercaptoethanol Fisher Cat#03446I Blasticidin Gibco Cat# R21001 RetroNectin Takara Cat# T100B AMPURE XP 60ML Thermo Fisher Scientific Cat#NC9933872 FORMALDEHYDE 20% 10X10ML Thermo Fisher Scientific Cat# NC9611804 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 559619 CellTiterGlo kit Promega Cat# G7570 Zymo Quick-RNA Miniprep Kit Zymo Research Cat# R1050 Mouse hematopoietic progenitor cell enrichment kit Stem Cell Technologies Cat# 19856 Nuclear Extract Kit Active Motif Cat# 40010 BCA assay Pierce Cat# 32106 (Continued on next page) e2 Cell Stem Cell 29, 298–314.e1–e9, February 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neon Transfection System 10 mL Kit Invitrogen Cat# MPK1025 RNeasy Plus Micro Kit Qiagen Cat# 74034 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio Cat# 634899 Taqman Assay human CDK4 probe Thermo Fisher Cat# 4453320 Assay ID: Hs00364847_m1 Taqman Assay Human NPM1 probe Thermo Fisher Cat# 4448892 Assay ID: Luciferase:Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 5-Bromo-2-deoxyuridine (BrdU) Sigma Cat# B5002 10058-F4 Sigma Cat# F3680 CU-T12-9 Tocris Bioscience Cat# 5414 Cycloheximide Santa Cruz Cat# sc-3508 Methocult GF M3434 Stem Cell Technologies Cat# 03434 Mouse Flt3-ligand cytokine PeproTech Cat# 250-31 Human IL-6 cytokine PeproTech Cat# 200-06 Mouse SCF cytokine PeproTech Cat# 250-03 Human TPO cytokine PeproTech Cat# 300-18 RPMI Fisher Cat# SH30027.01 DMEM Fisher Cat# SH30022FS Fetal Bovine Serum Atlanta Biologicals Cat# S11550 Penicillin/Steptomycin ThermoFisher Cat# SV30010 TransIT-LT1 transfection reagent Mirus Cat# MIR2306 IMDM Corning Cat# 10-016-CV Benzonase Millipore Cat# 70746 Nitrocellulose membrane Bio-Rad Cat# 162-0112 RIPA Lysis Buffer Santa Cruz Cat# sc-24948 Protein A/G Plus beads-Agarose Santa Cruz Cat# sc-2003 TrueCut Cas9 Protein v2 Thermo Fisher Cat# A36498 SuperScript II Reverse Transcriptase Invitrogen Cat# 18064014 TaqMan Universal PCR Master Mix Life Technologies Cat# 4324018 Recombinant c-Myc (WT) Abcam Cat# ab169901 Recombinant c-Myc (K148R) GeneScript N/A Recombinant TRAF6 Life Sensors Cat# UB312 Polybrene Millipore Cat# TR-1003-G EcoRI-HF New England Biolabs Cat# R3101L XhoI New England Biolabs Cat# R0146S XbaI New England Biolabs Cat# R0145S Quick Ligation kit New England Biolabs Cat# M2200S Puromycin Corning Cat# 61-385-RA Doxycycline Fisher Scientific Cat# BP26535 Poly(I:C) Tocris Bioscience Cat# 4287 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 5-fluorouracil (5-FU) CCHMC pharmacy NDC#63323-117-10 2-mercaptoethanol Fisher Cat#03446I Blasticidin Gibco Cat# R21001 RetroNectin Takara Cat# T100B AMPURE XP 60ML Thermo Fisher Scientific Cat#NC9933872 FORMALDEHYDE 20% 10X10ML Thermo Fisher Scientific Cat# NC9611804 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 559619 CellTiterGlo kit Promega Cat# G7570 Zymo Quick-RNA Miniprep Kit Zymo Research Cat# R1050 Mouse hematopoietic progenitor cell enrichment kit Stem Cell Technologies Cat# 19856 Nuclear Extract Kit Active Motif Cat# 40010 BCA assay Pierce Cat# 32106 (Continued on next page) e2 Cell Stem Cell 29, 298–314.e1–e9, February 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neon Transfection System 10 mL Kit Invitrogen Cat# MPK1025 RNeasy Plus Micro Kit Qiagen Cat# 74034 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio Cat# 634899 Taqman Assay human CDK4 probe Thermo Fisher Cat# 4453320 Assay ID: Hs00364847_m1 Taqman Assay Human NPM1 probe Thermo Fisher Cat# 4448892 Assay ID: Reporter Assay:Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 5-Bromo-2-deoxyuridine (BrdU) Sigma Cat# B5002 10058-F4 Sigma Cat# F3680 CU-T12-9 Tocris Bioscience Cat# 5414 Cycloheximide Santa Cruz Cat# sc-3508 Methocult GF M3434 Stem Cell Technologies Cat# 03434 Mouse Flt3-ligand cytokine PeproTech Cat# 250-31 Human IL-6 cytokine PeproTech Cat# 200-06 Mouse SCF cytokine PeproTech Cat# 250-03 Human TPO cytokine PeproTech Cat# 300-18 RPMI Fisher Cat# SH30027.01 DMEM Fisher Cat# SH30022FS Fetal Bovine Serum Atlanta Biologicals Cat# S11550 Penicillin/Steptomycin ThermoFisher Cat# SV30010 TransIT-LT1 transfection reagent Mirus Cat# MIR2306 IMDM Corning Cat# 10-016-CV Benzonase Millipore Cat# 70746 Nitrocellulose membrane Bio-Rad Cat# 162-0112 RIPA Lysis Buffer Santa Cruz Cat# sc-24948 Protein A/G Plus beads-Agarose Santa Cruz Cat# sc-2003 TrueCut Cas9 Protein v2 Thermo Fisher Cat# A36498 SuperScript II Reverse Transcriptase Invitrogen Cat# 18064014 TaqMan Universal PCR Master Mix Life Technologies Cat# 4324018 Recombinant c-Myc (WT) Abcam Cat# ab169901 Recombinant c-Myc (K148R) GeneScript N/A Recombinant TRAF6 Life Sensors Cat# UB312 Polybrene Millipore Cat# TR-1003-G EcoRI-HF New England Biolabs Cat# R3101L XhoI New England Biolabs Cat# R0146S XbaI New England Biolabs Cat# R0145S Quick Ligation kit New England Biolabs Cat# M2200S Puromycin Corning Cat# 61-385-RA Doxycycline Fisher Scientific Cat# BP26535 Poly(I:C) Tocris Bioscience Cat# 4287 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 5-fluorouracil (5-FU) CCHMC pharmacy NDC#63323-117-10 2-mercaptoethanol Fisher Cat#03446I Blasticidin Gibco Cat# R21001 RetroNectin Takara Cat# T100B AMPURE XP 60ML Thermo Fisher Scientific Cat#NC9933872 FORMALDEHYDE 20% 10X10ML Thermo Fisher Scientific Cat# NC9611804 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 559619 CellTiterGlo kit Promega Cat# G7570 Zymo Quick-RNA Miniprep Kit Zymo Research Cat# R1050 Mouse hematopoietic progenitor cell enrichment kit Stem Cell Technologies Cat# 19856 Nuclear Extract Kit Active Motif Cat# 40010 BCA assay Pierce Cat# 32106 (Continued on next page) e2 Cell Stem Cell 29, 298–314.e1–e9, February 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neon Transfection System 10 mL Kit Invitrogen Cat# MPK1025 RNeasy Plus Micro Kit Qiagen Cat# 74034 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio Cat# 634899 Taqman Assay human CDK4 probe Thermo Fisher Cat# 4453320 Assay ID: Hs00364847_m1 Taqman Assay Human NPM1 probe Thermo Fisher Cat# 4448892 Assay ID: DNA Sequencing:Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 5-Bromo-2-deoxyuridine (BrdU) Sigma Cat# B5002 10058-F4 Sigma Cat# F3680 CU-T12-9 Tocris Bioscience Cat# 5414 Cycloheximide Santa Cruz Cat# sc-3508 Methocult GF M3434 Stem Cell Technologies Cat# 03434 Mouse Flt3-ligand cytokine PeproTech Cat# 250-31 Human IL-6 cytokine PeproTech Cat# 200-06 Mouse SCF cytokine PeproTech Cat# 250-03 Human TPO cytokine PeproTech Cat# 300-18 RPMI Fisher Cat# SH30027.01 DMEM Fisher Cat# SH30022FS Fetal Bovine Serum Atlanta Biologicals Cat# S11550 Penicillin/Steptomycin ThermoFisher Cat# SV30010 TransIT-LT1 transfection reagent Mirus Cat# MIR2306 IMDM Corning Cat# 10-016-CV Benzonase Millipore Cat# 70746 Nitrocellulose membrane Bio-Rad Cat# 162-0112 RIPA Lysis Buffer Santa Cruz Cat# sc-24948 Protein A/G Plus beads-Agarose Santa Cruz Cat# sc-2003 TrueCut Cas9 Protein v2 Thermo Fisher Cat# A36498 SuperScript II Reverse Transcriptase Invitrogen Cat# 18064014 TaqMan Universal PCR Master Mix Life Technologies Cat# 4324018 Recombinant c-Myc (WT) Abcam Cat# ab169901 Recombinant c-Myc (K148R) GeneScript N/A Recombinant TRAF6 Life Sensors Cat# UB312 Polybrene Millipore Cat# TR-1003-G EcoRI-HF New England Biolabs Cat# R3101L XhoI New England Biolabs Cat# R0146S XbaI New England Biolabs Cat# R0145S Quick Ligation kit New England Biolabs Cat# M2200S Puromycin Corning Cat# 61-385-RA Doxycycline Fisher Scientific Cat# BP26535 Poly(I:C) Tocris Bioscience Cat# 4287 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 5-fluorouracil (5-FU) CCHMC pharmacy NDC#63323-117-10 2-mercaptoethanol Fisher Cat#03446I Blasticidin Gibco Cat# R21001 RetroNectin Takara Cat# T100B AMPURE XP 60ML Thermo Fisher Scientific Cat#NC9933872 FORMALDEHYDE 20% 10X10ML Thermo Fisher Scientific Cat# NC9611804 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 559619 CellTiterGlo kit Promega Cat# G7570 Zymo Quick-RNA Miniprep Kit Zymo Research Cat# R1050 Mouse hematopoietic progenitor cell enrichment kit Stem Cell Technologies Cat# 19856 Nuclear Extract Kit Active Motif Cat# 40010 BCA assay Pierce Cat# 32106 (Continued on next page) e2 Cell Stem Cell 29, 298–314.e1–e9, February 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neon Transfection System 10 mL Kit Invitrogen Cat# MPK1025 RNeasy Plus Micro Kit Qiagen Cat# 74034 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio Cat# 634899 Taqman Assay human CDK4 probe Thermo Fisher Cat# 4453320 Assay ID: Hs00364847_m1 Taqman Assay Human NPM1 probe Thermo Fisher Cat# 4448892 Assay ID: Immunoprecipitation:Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 5-Bromo-2-deoxyuridine (BrdU) Sigma Cat# B5002 10058-F4 Sigma Cat# F3680 CU-T12-9 Tocris Bioscience Cat# 5414 Cycloheximide Santa Cruz Cat# sc-3508 Methocult GF M3434 Stem Cell Technologies Cat# 03434 Mouse Flt3-ligand cytokine PeproTech Cat# 250-31 Human IL-6 cytokine PeproTech Cat# 200-06 Mouse SCF cytokine PeproTech Cat# 250-03 Human TPO cytokine PeproTech Cat# 300-18 RPMI Fisher Cat# SH30027.01 DMEM Fisher Cat# SH30022FS Fetal Bovine Serum Atlanta Biologicals Cat# S11550 Penicillin/Steptomycin ThermoFisher Cat# SV30010 TransIT-LT1 transfection reagent Mirus Cat# MIR2306 IMDM Corning Cat# 10-016-CV Benzonase Millipore Cat# 70746 Nitrocellulose membrane Bio-Rad Cat# 162-0112 RIPA Lysis Buffer Santa Cruz Cat# sc-24948 Protein A/G Plus beads-Agarose Santa Cruz Cat# sc-2003 TrueCut Cas9 Protein v2 Thermo Fisher Cat# A36498 SuperScript II Reverse Transcriptase Invitrogen Cat# 18064014 TaqMan Universal PCR Master Mix Life Technologies Cat# 4324018 Recombinant c-Myc (WT) Abcam Cat# ab169901 Recombinant c-Myc (K148R) GeneScript N/A Recombinant TRAF6 Life Sensors Cat# UB312 Polybrene Millipore Cat# TR-1003-G EcoRI-HF New England Biolabs Cat# R3101L XhoI New England Biolabs Cat# R0146S XbaI New England Biolabs Cat# R0145S Quick Ligation kit New England Biolabs Cat# M2200S Puromycin Corning Cat# 61-385-RA Doxycycline Fisher Scientific Cat# BP26535 Poly(I:C) Tocris Bioscience Cat# 4287 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 5-fluorouracil (5-FU) CCHMC pharmacy NDC#63323-117-10 2-mercaptoethanol Fisher Cat#03446I Blasticidin Gibco Cat# R21001 RetroNectin Takara Cat# T100B AMPURE XP 60ML Thermo Fisher Scientific Cat#NC9933872 FORMALDEHYDE 20% 10X10ML Thermo Fisher Scientific Cat# NC9611804 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 559619 CellTiterGlo kit Promega Cat# G7570 Zymo Quick-RNA Miniprep Kit Zymo Research Cat# R1050 Mouse hematopoietic progenitor cell enrichment kit Stem Cell Technologies Cat# 19856 Nuclear Extract Kit Active Motif Cat# 40010 BCA assay Pierce Cat# 32106 (Continued on next page) e2 Cell Stem Cell 29, 298–314.e1–e9, February 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neon Transfection System 10 mL Kit Invitrogen Cat# MPK1025 RNeasy Plus Micro Kit Qiagen Cat# 74034 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio Cat# 634899 Taqman Assay human CDK4 probe Thermo Fisher Cat# 4453320 Assay ID: Hs00364847_m1 Taqman Assay Human NPM1 probe Thermo Fisher Cat# 4448892 Assay ID: RNA sequencing:Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 5-Bromo-2-deoxyuridine (BrdU) Sigma Cat# B5002 10058-F4 Sigma Cat# F3680 CU-T12-9 Tocris Bioscience Cat# 5414 Cycloheximide Santa Cruz Cat# sc-3508 Methocult GF M3434 Stem Cell Technologies Cat# 03434 Mouse Flt3-ligand cytokine PeproTech Cat# 250-31 Human IL-6 cytokine PeproTech Cat# 200-06 Mouse SCF cytokine PeproTech Cat# 250-03 Human TPO cytokine PeproTech Cat# 300-18 RPMI Fisher Cat# SH30027.01 DMEM Fisher Cat# SH30022FS Fetal Bovine Serum Atlanta Biologicals Cat# S11550 Penicillin/Steptomycin ThermoFisher Cat# SV30010 TransIT-LT1 transfection reagent Mirus Cat# MIR2306 IMDM Corning Cat# 10-016-CV Benzonase Millipore Cat# 70746 Nitrocellulose membrane Bio-Rad Cat# 162-0112 RIPA Lysis Buffer Santa Cruz Cat# sc-24948 Protein A/G Plus beads-Agarose Santa Cruz Cat# sc-2003 TrueCut Cas9 Protein v2 Thermo Fisher Cat# A36498 SuperScript II Reverse Transcriptase Invitrogen Cat# 18064014 TaqMan Universal PCR Master Mix Life Technologies Cat# 4324018 Recombinant c-Myc (WT) Abcam Cat# ab169901 Recombinant c-Myc (K148R) GeneScript N/A Recombinant TRAF6 Life Sensors Cat# UB312 Polybrene Millipore Cat# TR-1003-G EcoRI-HF New England Biolabs Cat# R3101L XhoI New England Biolabs Cat# R0146S XbaI New England Biolabs Cat# R0145S Quick Ligation kit New England Biolabs Cat# M2200S Puromycin Corning Cat# 61-385-RA Doxycycline Fisher Scientific Cat# BP26535 Poly(I:C) Tocris Bioscience Cat# 4287 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 5-fluorouracil (5-FU) CCHMC pharmacy NDC#63323-117-10 2-mercaptoethanol Fisher Cat#03446I Blasticidin Gibco Cat# R21001 RetroNectin Takara Cat# T100B AMPURE XP 60ML Thermo Fisher Scientific Cat#NC9933872 FORMALDEHYDE 20% 10X10ML Thermo Fisher Scientific Cat# NC9611804 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 559619 CellTiterGlo kit Promega Cat# G7570 Zymo Quick-RNA Miniprep Kit Zymo Research Cat# R1050 Mouse hematopoietic progenitor cell enrichment kit Stem Cell Technologies Cat# 19856 Nuclear Extract Kit Active Motif Cat# 40010 BCA assay Pierce Cat# 32106 (Continued on next page) e2 Cell Stem Cell 29, 298–314.e1–e9, February 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neon Transfection System 10 mL Kit Invitrogen Cat# MPK1025 RNeasy Plus Micro Kit Qiagen Cat# 74034 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio Cat# 634899 Taqman Assay human CDK4 probe Thermo Fisher Cat# 4453320 Assay ID: Hs00364847_m1 Taqman Assay Human NPM1 probe Thermo Fisher Cat# 4448892 Assay ID: Microarray:Article Title: TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 5-Bromo-2-deoxyuridine (BrdU) Sigma Cat# B5002 10058-F4 Sigma Cat# F3680 CU-T12-9 Tocris Bioscience Cat# 5414 Cycloheximide Santa Cruz Cat# sc-3508 Methocult GF M3434 Stem Cell Technologies Cat# 03434 Mouse Flt3-ligand cytokine PeproTech Cat# 250-31 Human IL-6 cytokine PeproTech Cat# 200-06 Mouse SCF cytokine PeproTech Cat# 250-03 Human TPO cytokine PeproTech Cat# 300-18 RPMI Fisher Cat# SH30027.01 DMEM Fisher Cat# SH30022FS Fetal Bovine Serum Atlanta Biologicals Cat# S11550 Penicillin/Steptomycin ThermoFisher Cat# SV30010 TransIT-LT1 transfection reagent Mirus Cat# MIR2306 IMDM Corning Cat# 10-016-CV Benzonase Millipore Cat# 70746 Nitrocellulose membrane Bio-Rad Cat# 162-0112 RIPA Lysis Buffer Santa Cruz Cat# sc-24948 Protein A/G Plus beads-Agarose Santa Cruz Cat# sc-2003 TrueCut Cas9 Protein v2 Thermo Fisher Cat# A36498 SuperScript II Reverse Transcriptase Invitrogen Cat# 18064014 TaqMan Universal PCR Master Mix Life Technologies Cat# 4324018 Recombinant c-Myc (WT) Abcam Cat# ab169901 Recombinant c-Myc (K148R) GeneScript N/A Recombinant TRAF6 Life Sensors Cat# UB312 Polybrene Millipore Cat# TR-1003-G EcoRI-HF New England Biolabs Cat# R3101L XhoI New England Biolabs Cat# R0146S XbaI New England Biolabs Cat# R0145S Quick Ligation kit New England Biolabs Cat# M2200S Puromycin Corning Cat# 61-385-RA Doxycycline Fisher Scientific Cat# BP26535 Poly(I:C) Tocris Bioscience Cat# 4287 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 5-fluorouracil (5-FU) CCHMC pharmacy NDC#63323-117-10 2-mercaptoethanol Fisher Cat#03446I Blasticidin Gibco Cat# R21001 RetroNectin Takara Cat# T100B AMPURE XP 60ML Thermo Fisher Scientific Cat#NC9933872 FORMALDEHYDE 20% 10X10ML Thermo Fisher Scientific Cat# NC9611804 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 559619 CellTiterGlo kit Promega Cat# G7570 Zymo Quick-RNA Miniprep Kit Zymo Research Cat# R1050 Mouse hematopoietic progenitor cell enrichment kit Stem Cell Technologies Cat# 19856 Nuclear Extract Kit Active Motif Cat# 40010 BCA assay Pierce Cat# 32106 (Continued on next page) e2 Cell Stem Cell 29, 298–314.e1–e9, February 3, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neon Transfection System 10 mL Kit Invitrogen Cat# MPK1025 RNeasy Plus Micro Kit Qiagen Cat# 74034 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing Takara Bio Cat# 634899 Taqman Assay human CDK4 probe Thermo Fisher Cat# 4453320 Assay ID: Hs00364847_m1 Taqman Assay Human NPM1 probe Thermo Fisher Cat# 4448892 Assay ID: Gene Expression:Article Title: Ribosomal RNA methylation induced by MYC regulates translation Article Snippet: Quantitative real-time 566 PCRs were performed using TaqManTM Fast Universal PCR Master Mix (2X), no AmpEraseTM 567 UNG (ThermoFisher) or Fast SYBRTM Green Master Mix (ThermoFisher), on a StepOneTM 568 Real-Time PCR System (ThermoFisher) using automatic threshold detection. .. The following 569 TaqManTM Gene Expression Assays were used: ACTB, Hs01060665_g1; CDKN1A, 570 Hs00355782_m1; CDKN2A, Hs02902543_mH; FBL, Hs01070449_m1; MYC, 571 Hs00153408_m1; NPM1, Article Title: Regulation of translation by site-specific ribosomal RNA methylation. Article Snippet: Quantitative real-time PCRs were performed using TaqMan Fast Universal PCR Master Mix (2×), no AmpErase UNG (ThermoFisher) or Fast SYBR Green Master Mix (ThermoFisher) on a StepOne Real-Time PCR System (ThermoFisher) using automatic threshold detection. .. The following TaqMan Gene Expression Assays were used: ACTB, Hs01060665_g1; CDKN1A, Hs00355782_m1; CDKN2A, Hs02902543_mH; FBL, Hs01070449_m1; MYC, Hs00153408_m1; NPM1, Article Title: Mutated NPM1 in combination with overexpression of Meis1 or Hoxa9 is not sufficient to induce acute myeloid leukemia. Article Snippet: BM cells were stored in RNAprotect Cell Reagent (76526, Qiagen AB, Sollentuna, Sweden) until total RNA was extracted using the RNeasy Plus Mini kit (74136, Qiagen AB, Sollentuna, Sweden) and cDNA synthesized by SuperScript III First-Strand Synthesis SuperMix for qRTPCR (11752, Invitrogen, Life Technologies Europe BV, Stockholm, Sweden) all according to the manufacturer’s instructions. .. Gene expression was analyzed by TaqMan Gene Expression Assays; hNPM1, Article Title: Mutated NPM1 in combination with overexpression of Meis1 or Hoxa9 is not sufficient to induce acute myeloid leukemia Article Snippet: BM cells were stored in RNAprotect Cell Reagent (76526, Qiagen AB, Sollentuna, Sweden) until total RNA was extracted using the RNeasy Plus Mini kit (74136, Qiagen AB, Sollentuna, Sweden) and cDNA synthesized by SuperScript III First-Strand Synthesis SuperMix for qRT-PCR (11752, Invitrogen, Life Technologies Europe BV, Stockholm, Sweden) all according to the manufacturer’s instructions. .. Gene expression was analyzed by TaqMan Gene Expression Assays; hNPM1, Quantitative RT-PCR:Article Title: iTRAQ-Based Quantitative Proteomic Analysis of Nasopharyngeal Carcinoma. Article Snippet: 1 State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan, China 2 Department of Infectious Diseases, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China 3 Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China 4 Department of Hepatology and Infectious Diseases, Xiangya Hospital, Central South University, Changsha, Hunan, China 5 Department of Health Management Center, Xiangya Hospital, Central South University, Changsha, Hunan, China |
