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microplate reader  (Thermo Fisher)


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    Structured Review

    Thermo Fisher microplate reader
    Microplate Reader, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microplate+reader/Bovine+Serum+Albumin/pmc13320252-61-8-12
    Average 99 stars, based on 1 article reviews
    microplate reader - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: BMP-7 mRNA delivered by Fibrin–CaP scaffolds activates osteogenic programs in vivo as evidenced by transcriptomic and proteomic analyses
    Article Snippet: Absorbance was measured at 562 nm using a CLARIOSTAR plate reader (BMG Labtech). .. Fixed cells were then incubated for 1 h at room temperature in a blocking solution containing 1% bovine serum albumin (BSA; Thermo Fisher Scientific), 10% normal goat serum (R&D Systems), 0.3 M glycine, and 0.1% Tween-20 in DPBS to minimize nonspecific binding. .. Following blocking, cells were incubated overnight at 4 °C with a primary rabbit anti-human osteopontin antibody (1:200; Abcam).

    Blocking Assay:

    Article Title: BMP-7 mRNA delivered by Fibrin–CaP scaffolds activates osteogenic programs in vivo as evidenced by transcriptomic and proteomic analyses
    Article Snippet: Absorbance was measured at 562 nm using a CLARIOSTAR plate reader (BMG Labtech). .. Fixed cells were then incubated for 1 h at room temperature in a blocking solution containing 1% bovine serum albumin (BSA; Thermo Fisher Scientific), 10% normal goat serum (R&D Systems), 0.3 M glycine, and 0.1% Tween-20 in DPBS to minimize nonspecific binding. .. Following blocking, cells were incubated overnight at 4 °C with a primary rabbit anti-human osteopontin antibody (1:200; Abcam).

    Binding Assay:

    Article Title: BMP-7 mRNA delivered by Fibrin–CaP scaffolds activates osteogenic programs in vivo as evidenced by transcriptomic and proteomic analyses
    Article Snippet: Absorbance was measured at 562 nm using a CLARIOSTAR plate reader (BMG Labtech). .. Fixed cells were then incubated for 1 h at room temperature in a blocking solution containing 1% bovine serum albumin (BSA; Thermo Fisher Scientific), 10% normal goat serum (R&D Systems), 0.3 M glycine, and 0.1% Tween-20 in DPBS to minimize nonspecific binding. .. Following blocking, cells were incubated overnight at 4 °C with a primary rabbit anti-human osteopontin antibody (1:200; Abcam).

    other:

    Article Title: Neonatal muscle-derived extracellular vesicles containing miR-542-3p rejuvenate aged skeletal muscle via a functional microneedle patch
    Article Snippet: Immunoreactive bands were visualized using an enhanced chemiluminescence (ECL) reagent (CST, USA), and band intensities were quantified with ImageJ software by normalizing the signal of the target protein to that of internal control.

    Article Title: Antioxidant and anti-inflammatory effects of the extract from rhizomes of Belamcanda chinensis (L.) DC. on human keratinocyte HaCaT cells
    Article Snippet: The cell line identity was verified by matching the supplier-provided STR profile with the HaCaT reference profile from Ubigene ( https://www.ubigene.us/ ).

    Article Title: Synergistic antibacterial effect of photocatalytic coatings activated by visible light and photodynamic therapy for rapid peri-implantitis treatment
    Article Snippet: HGF cells were also used to verify cell viability using the LIVE/DEAD Cell Imaging Kit (Invitrogen, Life Technologies), following the manufacturer's protocol.

    Labeling:

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: .. Briefly, gels were formed and left in a solution of 1 mg mL −1 of fluorescein isothiocyanate (FITC) labeled bovine serum albumin (BSA, Thermo Fisher) in PBS overnight at room temperature. .. A Leica STELLARIS 5 confocal microscope with HCX PL APO CS × 63.0/1.40 OIL objective was used to bleach a region of interest with a white light laser (WLL) for 20 s (until the region was fully bleached).

    Centrifugation:

    Article Title: Recombinant bone morphogenetic protein-2 attenuates colorectal cancer progression by orchestrating Hippo pathway activation, Yes-associated protein inhibition, and epithelial-mesenchymal transition suppression.
    Article Snippet: The cells were homogenized in Radioimmunoprecipitation Assay lysis buffer (G-Biosciences) containing protease and phosphatase inhibitors (Sigma) and incubated on ice for 30 min with gentle agitation. .. After centrifugation at 13,200 rpm for 30 min at 4°C, the supernatants were obtained, and protein concentrations were measured using a bovine serum albumin-based quantification kit (Thermo Fisher Scientific). .. Equal amounts of protein (30 μg) were separated using SDS-PAGE and transferred to polyvinylidene fluoride membranes (Amersham).

    Flow Cytometry:

    Article Title: 4D piezoceramic-integrated scaffolds with bioelectric cues for skeletal muscle regeneration
    Article Snippet: Protein content was quantified using a Lowry assay (DC protein assay kit, Bio-Rad), according to the manufacturer's instructions. .. Absorbance was measured at 750 nm using a microplate reader (Multiskan FC, Thermo Scientific), and protein concentrations were determined using a standard curve generated from known concentrations of bovine serum albumin (BSA, PAA). .. The porosity of the scaffolds was evaluated both qualitatively, using Scanning Electron Microscopy (SEM) (FEI Quanta 200, ESEM), and quantitatively, using X-ray micro-computed tomography (micro-CT).

    Standard Curve:

    Article Title: 4D piezoceramic-integrated scaffolds with bioelectric cues for skeletal muscle regeneration
    Article Snippet: Protein content was quantified using a Lowry assay (DC protein assay kit, Bio-Rad), according to the manufacturer's instructions. .. Absorbance was measured at 750 nm using a microplate reader (Multiskan FC, Thermo Scientific), and protein concentrations were determined using a standard curve generated from known concentrations of bovine serum albumin (BSA, PAA). .. The porosity of the scaffolds was evaluated both qualitatively, using Scanning Electron Microscopy (SEM) (FEI Quanta 200, ESEM), and quantitatively, using X-ray micro-computed tomography (micro-CT).

    Generated:

    Article Title: 4D piezoceramic-integrated scaffolds with bioelectric cues for skeletal muscle regeneration
    Article Snippet: Protein content was quantified using a Lowry assay (DC protein assay kit, Bio-Rad), according to the manufacturer's instructions. .. Absorbance was measured at 750 nm using a microplate reader (Multiskan FC, Thermo Scientific), and protein concentrations were determined using a standard curve generated from known concentrations of bovine serum albumin (BSA, PAA). .. The porosity of the scaffolds was evaluated both qualitatively, using Scanning Electron Microscopy (SEM) (FEI Quanta 200, ESEM), and quantitatively, using X-ray micro-computed tomography (micro-CT).



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