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microplate based detection kit  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology microplate based detection kit
    Microplate Based Detection Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microplate+based+detection+kit/Pyruvate+Dehydrogenase+(PDH)+Activity+Assay+Kit/pm41920259-128-11-17
    Average 94 stars, based on 10 article reviews
    microplate based detection kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Shaoyao Gancao Decoction alleviates ulcerative colitis-associated secondary liver injury by inhibiting the phosphorylation of pyruvate dehydrogenase in macrophages.
    Article Snippet: Ethnopharmacological relevance: The classic formula Shaoyao Gancao Decoction (SGD) originates from "Treatise on Exogenous Febrile Disease".. Traditionally, it is used to treat ulcerative colitis (UC) and the secondary liver injury.. However, its mechanism is still unclear.

    Article Title: Ginsenoside Rg2 Targeting PDHE1 α to Improve High Glucose-Induced Hippocampal Neuronal Damage Based on CETSA Experiment.
    Article Snippet: Ginseng is recognized as a valuable source of compounds with medicinal properties.. Our previous research found that ginseng is rich in bioactive ABSTRACT Objective: To explore the effect of ginsenoside Rg2 (G-Rg2) targeting pyruvate dehydrogenase E1 component subunit alpha (PDHE1 ) on its downstream energy metabolism to repair damaged hippocampal neurons.. Methods: The binding affi nity between G-Rg2 and PDHE1 was obtained by molecular docking (MD) and surface plasmon resonance (SPR).

    Article Title: Targeting SLC25A33 Suppresses Vascular Smooth Muscle Cell Proliferation and Migration by Reducing Cytosolic mtDNA Levels: Implications for Occlusive Vascular Diseases
    Article Snippet: UTP measurements were performed in cytosolic and mitochondrial samples following the manufacturer’s protocol for the general uridine triphosphate enzyme-linked immunosorbent assay (ELISA) Kit (MyBioSource, San Diego, CA, USA). .. Pyruvate dehydrogenase (PDH) activity was assessed using the PDH Activity Assay Kit (Elabscience, Houston, TX, USA) according to the manufacturer’s instructions. ..

    Article Title: Targeting SLC25A33 Suppresses Vascular Smooth Muscle Cell Proliferation and Migration by Reducing Cytosolic mtDNA Levels: Implications for Occlusive Vascular Diseases.
    Article Snippet: UTP measurements were performed in cytosolic and mitochondrial samples following the manufacturer’s protocol for the general uridine triphosphate enzyme-linked immunosorbent assay (ELISA) Kit (MyBioSource, San Diego, CA, USA). .. Pyruvate dehydrogenase activity assay Pyruvate dehydrogenase (PDH) activity was assessed using the PDH Activity Assay Kit (Elabscience, Houston, TX, USA) according to the manufacturer’s instructions. ..

    Article Title: USP13 exacerbates the malignant progression of cervical cancer by inhibiting ECT2 ubiquitination and degradation.
    Article Snippet: .. Subsequently, in strict accordance with the instructions, the relative glucose uptake, lactate release, ATP level, lactate dehydrogenase (LDH) enzyme activity, and pyruvate dehydrogenase (PDH) enzyme activity of HeLa and Ca Ski cells were detected using a glucose uptake fluorometric assay kit (E-BC-F041, Elabscience, Wuhan, China), an L-lactic acid (LA) colorimetric assay kit (E-BC-K044-M, Elabscience), an ATP chemiluminescence assay kit (E-BC-F002, Elabscience), an LDH activity assay kit (E-BC-K046-M, Elabscience), and a PDH activity assay kit (E-BCK650-M, Elabscience), respectively. .. The Co-IP assay was performed using the PierceTM Classic Magnetic Bead IP/Co-IP kit (Invitrogen).

    Enzyme Activity Assay:

    Article Title: Shaoyao Gancao Decoction alleviates ulcerative colitis-associated secondary liver injury by inhibiting the phosphorylation of pyruvate dehydrogenase in macrophages.
    Article Snippet: Ethnopharmacological relevance: The classic formula Shaoyao Gancao Decoction (SGD) originates from "Treatise on Exogenous Febrile Disease".. Traditionally, it is used to treat ulcerative colitis (UC) and the secondary liver injury.. However, its mechanism is still unclear.

    Colorimetric Assay:

    Article Title: USP13 exacerbates the malignant progression of cervical cancer by inhibiting ECT2 ubiquitination and degradation.
    Article Snippet: .. Subsequently, in strict accordance with the instructions, the relative glucose uptake, lactate release, ATP level, lactate dehydrogenase (LDH) enzyme activity, and pyruvate dehydrogenase (PDH) enzyme activity of HeLa and Ca Ski cells were detected using a glucose uptake fluorometric assay kit (E-BC-F041, Elabscience, Wuhan, China), an L-lactic acid (LA) colorimetric assay kit (E-BC-K044-M, Elabscience), an ATP chemiluminescence assay kit (E-BC-F002, Elabscience), an LDH activity assay kit (E-BC-K046-M, Elabscience), and a PDH activity assay kit (E-BCK650-M, Elabscience), respectively. .. The Co-IP assay was performed using the PierceTM Classic Magnetic Bead IP/Co-IP kit (Invitrogen).

    Chemiluminescence Immunoassay:

    Article Title: USP13 exacerbates the malignant progression of cervical cancer by inhibiting ECT2 ubiquitination and degradation.
    Article Snippet: .. Subsequently, in strict accordance with the instructions, the relative glucose uptake, lactate release, ATP level, lactate dehydrogenase (LDH) enzyme activity, and pyruvate dehydrogenase (PDH) enzyme activity of HeLa and Ca Ski cells were detected using a glucose uptake fluorometric assay kit (E-BC-F041, Elabscience, Wuhan, China), an L-lactic acid (LA) colorimetric assay kit (E-BC-K044-M, Elabscience), an ATP chemiluminescence assay kit (E-BC-F002, Elabscience), an LDH activity assay kit (E-BC-K046-M, Elabscience), and a PDH activity assay kit (E-BCK650-M, Elabscience), respectively. .. The Co-IP assay was performed using the PierceTM Classic Magnetic Bead IP/Co-IP kit (Invitrogen).



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    R&D Systems microplate reader based titertacs in situ apoptosis detection kit
    Figure 5. TXNDC5 promotes colorectal cancer cell proliferation, colony formation and survival in vitro. RKO and HCT‑116 cells were transfected with a specific siRNA (siTXNDC5) to knockdown TXNDC5. Cells transfected with siScr were used as a control. (A) MTT assays (*P<0.0001), (B and C) colony formation assays(*P<0.0001) and (D) <t>apoptosis</t> assays (**P=0.0020; ***P=0.0004) were performed in RKO and HCT‑116 cells transfected with siTXNDC5 or siScr under H and N. H/Hyp, hypoxia; N/Nom, normoxia; OD, optical density; siRNA/si, small interfering RNA; siScr, scrambled control siRNA; TXNDC5, thioredoxin domain‑containing 5.
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    Image Search Results


    Figure 5. TXNDC5 promotes colorectal cancer cell proliferation, colony formation and survival in vitro. RKO and HCT‑116 cells were transfected with a specific siRNA (siTXNDC5) to knockdown TXNDC5. Cells transfected with siScr were used as a control. (A) MTT assays (*P<0.0001), (B and C) colony formation assays(*P<0.0001) and (D) apoptosis assays (**P=0.0020; ***P=0.0004) were performed in RKO and HCT‑116 cells transfected with siTXNDC5 or siScr under H and N. H/Hyp, hypoxia; N/Nom, normoxia; OD, optical density; siRNA/si, small interfering RNA; siScr, scrambled control siRNA; TXNDC5, thioredoxin domain‑containing 5.

    Journal: International journal of molecular medicine

    Article Title: Role of TXNDC5 in tumorigenesis of colorectal cancer cells: In vivo and in vitro evidence.

    doi: 10.3892/ijmm.2018.3664

    Figure Lengend Snippet: Figure 5. TXNDC5 promotes colorectal cancer cell proliferation, colony formation and survival in vitro. RKO and HCT‑116 cells were transfected with a specific siRNA (siTXNDC5) to knockdown TXNDC5. Cells transfected with siScr were used as a control. (A) MTT assays (*P<0.0001), (B and C) colony formation assays(*P<0.0001) and (D) apoptosis assays (**P=0.0020; ***P=0.0004) were performed in RKO and HCT‑116 cells transfected with siTXNDC5 or siScr under H and N. H/Hyp, hypoxia; N/Nom, normoxia; OD, optical density; siRNA/si, small interfering RNA; siScr, scrambled control siRNA; TXNDC5, thioredoxin domain‑containing 5.

    Article Snippet: Cell apoptosis assay. cells were seeded at 1x105 cells/well in 96-well tissue culture plates. cell apoptosis was measured at 24 h using a microplate reader-based titertacS in situ apoptosis detection kit (r&d Systems, inc., minneapolis, mn, uSa) according to the manufacturer's protocol. the percentage of apoptotic cells was calculated.

    Techniques: In Vitro, Transfection, Knockdown, Control, Small Interfering RNA

    Figure 7. TXNDC5 increases the expression of ER stress and apoptotic markers. (A) RKO and HCT‑116 cells were transfected with a specific siRNA (siTXNDC5) to knockdown TXNDC5. Cells transfected with siScr were used as a control. Reactive oxygen species production was mea- sured in cells under H and N for 24 h. *P=0.0004; **P=0.0010. (B) In RKO and HCT‑116 cells transfected with siTXNDC5 or siScr under H and N, western blot analyses were used to detect the protein expression levels of ER stress (CHOP, GRP78 and ATF4), apoptosis (Bax and cleaved caspase‑8) and survival (Bcl‑2) markers. β‑actin was used as a loading control. Density of the blots was normalized against that of the β‑actin blot, in order to obtain relative blot density. *P<0.05. ATF4, activating transcription factor 4; Bax, Bcl‑2‑associated X protein; Bcl‑2, B‑cell lymphoma 2; c‑C8, caspase‑8; CHOP, CCAAT‑enhancer‑binding protein homologous protein; GRP78, glucose‑regulated protein 78; DCF, dichlorodihydrofluorescein; ER, endoplasmic reticulum; H, hypoxia; N, normoxia; siRNA/si, small interfering RNA; siScr, scrambled control siRNA; TXNDC5, thioredoxin domain‑containing 5.

    Journal: International journal of molecular medicine

    Article Title: Role of TXNDC5 in tumorigenesis of colorectal cancer cells: In vivo and in vitro evidence.

    doi: 10.3892/ijmm.2018.3664

    Figure Lengend Snippet: Figure 7. TXNDC5 increases the expression of ER stress and apoptotic markers. (A) RKO and HCT‑116 cells were transfected with a specific siRNA (siTXNDC5) to knockdown TXNDC5. Cells transfected with siScr were used as a control. Reactive oxygen species production was mea- sured in cells under H and N for 24 h. *P=0.0004; **P=0.0010. (B) In RKO and HCT‑116 cells transfected with siTXNDC5 or siScr under H and N, western blot analyses were used to detect the protein expression levels of ER stress (CHOP, GRP78 and ATF4), apoptosis (Bax and cleaved caspase‑8) and survival (Bcl‑2) markers. β‑actin was used as a loading control. Density of the blots was normalized against that of the β‑actin blot, in order to obtain relative blot density. *P<0.05. ATF4, activating transcription factor 4; Bax, Bcl‑2‑associated X protein; Bcl‑2, B‑cell lymphoma 2; c‑C8, caspase‑8; CHOP, CCAAT‑enhancer‑binding protein homologous protein; GRP78, glucose‑regulated protein 78; DCF, dichlorodihydrofluorescein; ER, endoplasmic reticulum; H, hypoxia; N, normoxia; siRNA/si, small interfering RNA; siScr, scrambled control siRNA; TXNDC5, thioredoxin domain‑containing 5.

    Article Snippet: Cell apoptosis assay. cells were seeded at 1x105 cells/well in 96-well tissue culture plates. cell apoptosis was measured at 24 h using a microplate reader-based titertacS in situ apoptosis detection kit (r&d Systems, inc., minneapolis, mn, uSa) according to the manufacturer's protocol. the percentage of apoptotic cells was calculated.

    Techniques: Expressing, Transfection, Knockdown, Control, Western Blot, Small Interfering RNA