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lip39 k01  (Eagle Biosciences)


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    Structured Review

    Eagle Biosciences lip39 k01
    Lip39 K01, supplied by Eagle Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microplate+based+detection+kit/LPO+Microplate+Based+Assay+Kit/pm41476305-101-11-13
    Average 93 stars, based on 12 article reviews
    lip39 k01 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: Silver nanoparticles loaded with pomegranate peel extract and hyaluronic acid mediate recovery of cutaneous wounds infected with Candida albicans
    Article Snippet: Moreover, ELISA assays were utilized to estimate the concentrations of transforming growth factor-beta 1 (TGF-β1) (MultiSciences, Biotech, Co., Hangzhou, China), vascular endothelial growth factor (VEGF) (Abcam, Cambridge, USA), angiopoietin-1 (Ang-1) (Elabscience, USA) and matrix metalloproteinase-9 (MMP-9) (Elabscience, USA) on days 5 and 15 post-treatment. .. On day 10 post-treatment, the skin tissue homogenates were used to estimate the levels of lipid peroxides reflected by malondialdehyde (MDA) via a specific commercial colorimetric kit (Cat. No. LIP39-K01) purchased from Eagle Biosciences, Inc. (Boston, USA) and total antioxidant capacity (TAC) was calculated via a Cayman TAC assay kit (Cayman Chemical Co., Ann Arbor, USA) following manufacturer’s instructions. .. Furthermore, ELISA kits (MyBioSource; San Diego, USA) were utilized for the measurement of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) activity (Cat. No. MBS028183, MBS006963 and MBS036924 respectively) according to manufacturer’s protocols.

    Article Title: Diosmin mitigates dexamethasone-induced osteoporosis in vivo: Role of Runx2, RANKL/OPG, and oxidative stress.
    Article Snippet: To determine the antioxidative status of bone, glutathione (GSH) and superoxide dismutase (SOD) contents were measured utilizing ELISA kits (GSH: Eagle Biosciences, Cat# GLU39-K01; SOD: Cusabio, Cat# CSBEL022397RA). .. Lipid peroxidation, the oxidative stress biomarker, was assessed by measuring malondialdehyde (MDA) content colorimetrically in bone tissue using a kit (Eagle Biosciences, Cat# LIP39-K01). .. Statistical analysis was carried out using GraphPad Prism version 5.0 (San Diego, CA).

    Article Title: Mangiferin: a novel hepatoprotective for liver cirrhosis via modulation of histological, cellular, biochemical, inflammatory markers and oxidative stress.
    Article Snippet: .. The levels of MDA were measured by using kit (Cat. No. LIP39-K01; from Eagle Biosciences, Boston, MA, USA), and CAT, and SOD were measured using kits from Cayman CAT assay kit (Cayman Ann Arbor, MI, USA) were estimated colorimetrically in liver homogenate according to the methods described by the manufacturer. .. Blood samples were used to estimate the liver function parameters including ALT (Cat. No. GOT111060), AST (Cat. No. GPT113100), ALP, bilirubin, albumin (Cat. No. ALB100250), and total protein (Cat. No. TP116250) were purchased from BioMed Diagnostics (Egy-Chem for lab technology Badr City, Egypt) were quantified spectrophotometrically in serum, using the previously mentioned commercial kits, following the manufacturer’s instructions.

    Article Title: Silver nanoparticles loaded with pomegranate peel extract and hyaluronic acid mediate recovery of cutaneous wounds infected with Candida albicans .
    Article Snippet: Moreover, ELISA assays were utilized to estimate the concentrations of transforming growth factor-beta 1 (TGF-b1) (MultiSciences, Biotech, Co., Hangzhou, China), vascular endothelial growth factor (VEGF) (Abcam, Cambridge, USA), angiopoietin-1 (Ang-1) (Elabscience, USA) and matrix metalloproteinase-9 (MMP9) (Elabscience, USA) on days 5 and 15 post-treatment. .. On day 10 post-treatment, the skin tissue homogenates were used to estimate the levels of lipid peroxides reflected by malondialdehyde Frontiers in Cellular and Infection Microbiology 07 (MDA) via a specific commercial colorimetric kit (Cat. No. LIP39K01) purchased from Eagle Biosciences, Inc. (Boston, USA) and total antioxidant capacity (TAC) was calculated via a Cayman TAC assay kit (Cayman Chemical Co., Ann Arbor, USA) following manufacturer ’s instructions. .. Furthermore, ELISA kits (MyBioSource; San Diego, USA) were utilized for the measurement of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) activity (Cat. No. MBS028183, MBS006963 and MBS036924 respectively) according to manufacturer’s protocols.

    Article Title: Possible mechanisms underlying the neuroprotective effects of gold nanoparticles and alpha-lipoic acid mixture on brain damage induced by radiation: A subacute study in rats
    Article Snippet: Building on previously published work, which revealed that acute treatment with a mixture of gold nanoparticles (AuNPs) and alpha-lipoic acid (ALA) alleviated brain injury induced by radiation in male rats, the current research aimed to explore the possible mechanisms underlying the neuroprotective effects of AuNPs-ALA mixture on radiation-induced brain injury using a subacute treatment protocol.. Male Wistar rats received AuNPs-ALA mixture orally for 14 days.. One hour after administration of the last dosage of AuNPs-ALA mixture, rats were exposed to one dose of 7Gy whole-body gamma radiation.

    Biomarker Discovery:

    Article Title: Diosmin mitigates dexamethasone-induced osteoporosis in vivo: Role of Runx2, RANKL/OPG, and oxidative stress.
    Article Snippet: To determine the antioxidative status of bone, glutathione (GSH) and superoxide dismutase (SOD) contents were measured utilizing ELISA kits (GSH: Eagle Biosciences, Cat# GLU39-K01; SOD: Cusabio, Cat# CSBEL022397RA). .. Lipid peroxidation, the oxidative stress biomarker, was assessed by measuring malondialdehyde (MDA) content colorimetrically in bone tissue using a kit (Eagle Biosciences, Cat# LIP39-K01). .. Statistical analysis was carried out using GraphPad Prism version 5.0 (San Diego, CA).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Mangiferin: a novel hepatoprotective for liver cirrhosis via modulation of histological, cellular, biochemical, inflammatory markers and oxidative stress.
    Article Snippet: .. The levels of MDA were measured by using kit (Cat. No. LIP39-K01; from Eagle Biosciences, Boston, MA, USA), and CAT, and SOD were measured using kits from Cayman CAT assay kit (Cayman Ann Arbor, MI, USA) were estimated colorimetrically in liver homogenate according to the methods described by the manufacturer. .. Blood samples were used to estimate the liver function parameters including ALT (Cat. No. GOT111060), AST (Cat. No. GPT113100), ALP, bilirubin, albumin (Cat. No. ALB100250), and total protein (Cat. No. TP116250) were purchased from BioMed Diagnostics (Egy-Chem for lab technology Badr City, Egypt) were quantified spectrophotometrically in serum, using the previously mentioned commercial kits, following the manufacturer’s instructions.

    Infection:

    Article Title: Silver nanoparticles loaded with pomegranate peel extract and hyaluronic acid mediate recovery of cutaneous wounds infected with Candida albicans .
    Article Snippet: Moreover, ELISA assays were utilized to estimate the concentrations of transforming growth factor-beta 1 (TGF-b1) (MultiSciences, Biotech, Co., Hangzhou, China), vascular endothelial growth factor (VEGF) (Abcam, Cambridge, USA), angiopoietin-1 (Ang-1) (Elabscience, USA) and matrix metalloproteinase-9 (MMP9) (Elabscience, USA) on days 5 and 15 post-treatment. .. On day 10 post-treatment, the skin tissue homogenates were used to estimate the levels of lipid peroxides reflected by malondialdehyde Frontiers in Cellular and Infection Microbiology 07 (MDA) via a specific commercial colorimetric kit (Cat. No. LIP39K01) purchased from Eagle Biosciences, Inc. (Boston, USA) and total antioxidant capacity (TAC) was calculated via a Cayman TAC assay kit (Cayman Chemical Co., Ann Arbor, USA) following manufacturer ’s instructions. .. Furthermore, ELISA kits (MyBioSource; San Diego, USA) were utilized for the measurement of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) activity (Cat. No. MBS028183, MBS006963 and MBS036924 respectively) according to manufacturer’s protocols.

    Activity Assay:

    Article Title: Possible mechanisms underlying the neuroprotective effects of gold nanoparticles and alpha-lipoic acid mixture on brain damage induced by radiation: A subacute study in rats
    Article Snippet: Building on previously published work, which revealed that acute treatment with a mixture of gold nanoparticles (AuNPs) and alpha-lipoic acid (ALA) alleviated brain injury induced by radiation in male rats, the current research aimed to explore the possible mechanisms underlying the neuroprotective effects of AuNPs-ALA mixture on radiation-induced brain injury using a subacute treatment protocol.. Male Wistar rats received AuNPs-ALA mixture orally for 14 days.. One hour after administration of the last dosage of AuNPs-ALA mixture, rats were exposed to one dose of 7Gy whole-body gamma radiation.



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    Figure 5. TXNDC5 promotes colorectal cancer cell proliferation, colony formation and survival in vitro. RKO and HCT‑116 cells were transfected with a specific siRNA (siTXNDC5) to knockdown TXNDC5. Cells transfected with siScr were used as a control. (A) MTT assays (*P<0.0001), (B and C) colony formation assays(*P<0.0001) and (D) <t>apoptosis</t> assays (**P=0.0020; ***P=0.0004) were performed in RKO and HCT‑116 cells transfected with siTXNDC5 or siScr under H and N. H/Hyp, hypoxia; N/Nom, normoxia; OD, optical density; siRNA/si, small interfering RNA; siScr, scrambled control siRNA; TXNDC5, thioredoxin domain‑containing 5.
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    Image Search Results


    Figure 5. TXNDC5 promotes colorectal cancer cell proliferation, colony formation and survival in vitro. RKO and HCT‑116 cells were transfected with a specific siRNA (siTXNDC5) to knockdown TXNDC5. Cells transfected with siScr were used as a control. (A) MTT assays (*P<0.0001), (B and C) colony formation assays(*P<0.0001) and (D) apoptosis assays (**P=0.0020; ***P=0.0004) were performed in RKO and HCT‑116 cells transfected with siTXNDC5 or siScr under H and N. H/Hyp, hypoxia; N/Nom, normoxia; OD, optical density; siRNA/si, small interfering RNA; siScr, scrambled control siRNA; TXNDC5, thioredoxin domain‑containing 5.

    Journal: International journal of molecular medicine

    Article Title: Role of TXNDC5 in tumorigenesis of colorectal cancer cells: In vivo and in vitro evidence.

    doi: 10.3892/ijmm.2018.3664

    Figure Lengend Snippet: Figure 5. TXNDC5 promotes colorectal cancer cell proliferation, colony formation and survival in vitro. RKO and HCT‑116 cells were transfected with a specific siRNA (siTXNDC5) to knockdown TXNDC5. Cells transfected with siScr were used as a control. (A) MTT assays (*P<0.0001), (B and C) colony formation assays(*P<0.0001) and (D) apoptosis assays (**P=0.0020; ***P=0.0004) were performed in RKO and HCT‑116 cells transfected with siTXNDC5 or siScr under H and N. H/Hyp, hypoxia; N/Nom, normoxia; OD, optical density; siRNA/si, small interfering RNA; siScr, scrambled control siRNA; TXNDC5, thioredoxin domain‑containing 5.

    Article Snippet: Cell apoptosis assay. cells were seeded at 1x105 cells/well in 96-well tissue culture plates. cell apoptosis was measured at 24 h using a microplate reader-based titertacS in situ apoptosis detection kit (r&d Systems, inc., minneapolis, mn, uSa) according to the manufacturer's protocol. the percentage of apoptotic cells was calculated.

    Techniques: In Vitro, Transfection, Knockdown, Control, Small Interfering RNA

    Figure 7. TXNDC5 increases the expression of ER stress and apoptotic markers. (A) RKO and HCT‑116 cells were transfected with a specific siRNA (siTXNDC5) to knockdown TXNDC5. Cells transfected with siScr were used as a control. Reactive oxygen species production was mea- sured in cells under H and N for 24 h. *P=0.0004; **P=0.0010. (B) In RKO and HCT‑116 cells transfected with siTXNDC5 or siScr under H and N, western blot analyses were used to detect the protein expression levels of ER stress (CHOP, GRP78 and ATF4), apoptosis (Bax and cleaved caspase‑8) and survival (Bcl‑2) markers. β‑actin was used as a loading control. Density of the blots was normalized against that of the β‑actin blot, in order to obtain relative blot density. *P<0.05. ATF4, activating transcription factor 4; Bax, Bcl‑2‑associated X protein; Bcl‑2, B‑cell lymphoma 2; c‑C8, caspase‑8; CHOP, CCAAT‑enhancer‑binding protein homologous protein; GRP78, glucose‑regulated protein 78; DCF, dichlorodihydrofluorescein; ER, endoplasmic reticulum; H, hypoxia; N, normoxia; siRNA/si, small interfering RNA; siScr, scrambled control siRNA; TXNDC5, thioredoxin domain‑containing 5.

    Journal: International journal of molecular medicine

    Article Title: Role of TXNDC5 in tumorigenesis of colorectal cancer cells: In vivo and in vitro evidence.

    doi: 10.3892/ijmm.2018.3664

    Figure Lengend Snippet: Figure 7. TXNDC5 increases the expression of ER stress and apoptotic markers. (A) RKO and HCT‑116 cells were transfected with a specific siRNA (siTXNDC5) to knockdown TXNDC5. Cells transfected with siScr were used as a control. Reactive oxygen species production was mea- sured in cells under H and N for 24 h. *P=0.0004; **P=0.0010. (B) In RKO and HCT‑116 cells transfected with siTXNDC5 or siScr under H and N, western blot analyses were used to detect the protein expression levels of ER stress (CHOP, GRP78 and ATF4), apoptosis (Bax and cleaved caspase‑8) and survival (Bcl‑2) markers. β‑actin was used as a loading control. Density of the blots was normalized against that of the β‑actin blot, in order to obtain relative blot density. *P<0.05. ATF4, activating transcription factor 4; Bax, Bcl‑2‑associated X protein; Bcl‑2, B‑cell lymphoma 2; c‑C8, caspase‑8; CHOP, CCAAT‑enhancer‑binding protein homologous protein; GRP78, glucose‑regulated protein 78; DCF, dichlorodihydrofluorescein; ER, endoplasmic reticulum; H, hypoxia; N, normoxia; siRNA/si, small interfering RNA; siScr, scrambled control siRNA; TXNDC5, thioredoxin domain‑containing 5.

    Article Snippet: Cell apoptosis assay. cells were seeded at 1x105 cells/well in 96-well tissue culture plates. cell apoptosis was measured at 24 h using a microplate reader-based titertacS in situ apoptosis detection kit (r&d Systems, inc., minneapolis, mn, uSa) according to the manufacturer's protocol. the percentage of apoptotic cells was calculated.

    Techniques: Expressing, Transfection, Knockdown, Control, Western Blot, Small Interfering RNA